postinfection  (Millipore)


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    Structured Review

    Millipore postinfection
    Recovery of viable F. tularensis bacteria from A. castellanii cysts 21 days <t>postinfection.</t>
    Postinfection, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/postinfection/product/Millipore
    Average 93 stars, based on 710 article reviews
    Price from $9.99 to $1999.99
    postinfection - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Francisella tularensis Type A Strains Cause the Rapid Encystment of Acanthamoeba castellanii and Survive in Amoebal Cysts for Three Weeks Postinfection ▿"

    Article Title: Francisella tularensis Type A Strains Cause the Rapid Encystment of Acanthamoeba castellanii and Survive in Amoebal Cysts for Three Weeks Postinfection ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01829-09

    Recovery of viable F. tularensis bacteria from A. castellanii cysts 21 days postinfection.
    Figure Legend Snippet: Recovery of viable F. tularensis bacteria from A. castellanii cysts 21 days postinfection.

    Techniques Used:

    Quantification of the encystment of A. castellanii at 2 h postinfection with F. tularensis strains. Data points and error bars represent the means and standard deviations, respectively, of three random field counts of assays done in triplicate from representative
    Figure Legend Snippet: Quantification of the encystment of A. castellanii at 2 h postinfection with F. tularensis strains. Data points and error bars represent the means and standard deviations, respectively, of three random field counts of assays done in triplicate from representative

    Techniques Used:

    Transmission electron micrographs of A. castellanii amoebae infected with F. tularensis strains. Ft-1 (A) is present in spacious vacuoles, while LVS (B) is present in tight vacuoles at 2 h postinfection; long black arrows point to spacious vacuoles, and
    Figure Legend Snippet: Transmission electron micrographs of A. castellanii amoebae infected with F. tularensis strains. Ft-1 (A) is present in spacious vacuoles, while LVS (B) is present in tight vacuoles at 2 h postinfection; long black arrows point to spacious vacuoles, and

    Techniques Used: Transmission Assay, Infection

    Immunofluorescence z-stack projections of A. castellanii trophozoites infected with LVS expressing GFP for 2 h (a, b, and c) and NOV stained with an anti- F. tularensis antibody (green) (f, g, and h) after 2 h and 30 min, respectively. At 2 h postinfection,
    Figure Legend Snippet: Immunofluorescence z-stack projections of A. castellanii trophozoites infected with LVS expressing GFP for 2 h (a, b, and c) and NOV stained with an anti- F. tularensis antibody (green) (f, g, and h) after 2 h and 30 min, respectively. At 2 h postinfection,

    Techniques Used: Immunofluorescence, Infection, Expressing, Staining

    Pathogenic F. tularensis strains are present in spacious vacuoles at 30 min postinfection.
    Figure Legend Snippet: Pathogenic F. tularensis strains are present in spacious vacuoles at 30 min postinfection.

    Techniques Used:

    Related Articles

    Sonication:

    Article Title: Stormwater runoff drives viral community composition changes in inland freshwaters
    Article Snippet: .. Subsamples (50 ml) of the sonicated slurry were centrifuged at 5000 ×g for 20 min to pellet suspended solids and the supernatant was filtered through 0.22 μm Sterivex syringe filters (Millipore, Billerica, MA) to remove bacteria. ..

    Injection:

    Article Title: Role of Green Macroalgae Enteromorpha Prolifera Polyphenols in the Modulation of Gene Expression and Intestinal Microflora Profiles in Type 2 Diabetic Mice
    Article Snippet: .. Ultra-Performance Liquid Chromatography-Mass Spectrometric (UPLC-MS) Analysis After centrifuging at 12,000 rpm for 10 min at 4 °C, the extract was filtered through Syringe filters (PTFE, 0.22 μm pore and 13 mm diameter, Millipore Millex, Billerica, MA, USA) and then injected to UPLC system (Waters Co., Milford, MA, USA). .. Chromatographic separations were carried out on a Waters Acquity UPLC I-Class with C18 column (1.8 μm, 2.1*100 mm, Waters Co.).

    Article Title: Comparative Study of Chemical, Biochemical Characteristic and ATR-FTIR Analysis of Seeds, Oil and Flour of the Edible Fedora Cultivar Hemp (Cannabis sativa L.)
    Article Snippet: .. The organic phase was filtered using Millex 0.45 μm PVDF disposable syringe filters (EMD Millipore Corp., Billerica, MA, USA) and 1 μL was directly injected into the gas chromatograph for analysis. .. GC-FID Analysis of Fatty Acids FAME, prepared as reported above, were analysed with a Trace GC gas chromatographer (Thermo Scientific, Inc., San Jose, CA, USA) equipped with an FID (Flame Ionization Detector), using an SP-2560 (100 m × 0.25 mm × 0.20 μm) capillary column (Supelco, Sigma-Aldrich).

    other:

    Article Title: Self-assembly of polyphosphazene immunoadjuvant with poly(ethylene oxide) enables advanced nanoscale delivery modalities and regulated pH-dependent cellular membrane activity
    Article Snippet: Formulation components were filtered using 0.22 μm Millex syringe filters (EMD Millipore, Billerica, MA) before mixing.

    Chromatography:

    Article Title: Role of Green Macroalgae Enteromorpha Prolifera Polyphenols in the Modulation of Gene Expression and Intestinal Microflora Profiles in Type 2 Diabetic Mice
    Article Snippet: .. Ultra-Performance Liquid Chromatography-Mass Spectrometric (UPLC-MS) Analysis After centrifuging at 12,000 rpm for 10 min at 4 °C, the extract was filtered through Syringe filters (PTFE, 0.22 μm pore and 13 mm diameter, Millipore Millex, Billerica, MA, USA) and then injected to UPLC system (Waters Co., Milford, MA, USA). .. Chromatographic separations were carried out on a Waters Acquity UPLC I-Class with C18 column (1.8 μm, 2.1*100 mm, Waters Co.).

    Lysis:

    Article Title: Architecture and RNA binding of the human negative elongation factor
    Article Snippet: .. Clarified lysates were filtered through 0.8 µm syringe filters (Millipore) and applied to a 5 mlL HisTrap columns (GE Healthcare) equilibrated in lysis buffer. .. HisTrap columns were washed with 10CV of lysis buffer followed by 5CV of high salt wash buffer (800 mM NaCl, 20 mM Na•HEPES pH 7.4, 10% glycerol (v/v), 1 mM DTT, 30 mM imidazole pH 8.0, 0.284 µg/ml leupeptin, 1.37 µg/ml pepstatin A, 0.17 mg/ml PMSF, 0.33 mg/ml benzamidine) and 5CV of lysis buffer.

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