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Bio-Rad amplicon detection
Amplicon Detection, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplicon detection/product/Bio-Rad
Average 89 stars, based on 9 article reviews
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amplicon detection - by Bioz Stars, 2020-09
89/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: Diagnostic Performance of Schistosoma Real-Time PCR in Urine Samples from Kenyan Children Infected with Schistosoma haematobium: Day-to-day Variation and Follow-up after Praziquantel Treatment
Article Snippet: .. A CFX96 real-time PCR detection system (Bio-Rad) was used for amplification and amplicon detection, and CFX Manager version 1.6.514 (Bio-Rad) for related data analysis. .. The PCR output is expressed as Cyclic-threshold (Ct-) values.

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: No role of IFITM3 in brain tumor formation in vivo
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the house-keeping genes elongation factor 1α (Ef1α), β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase activation protein zeta (YHWAZ).

Article Title: Neurovascular EGFL7 regulates adult neurogenesis in the subventricular zone and thereby affects olfactory perception
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad, Hercules, CA, USA). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the housekeeping genes (HKG) glyceraldehyde-3-phosphate dehydrogenase , RNA polymerase II (RNAPII) and ribosomal protein S13 (Rps13).

Article Title: Increased MMPs expression and decreased contraction in the rat myometrium during pregnancy and in response to prolonged stretch and sex hormones
Article Snippet: .. Quantification of gene expression was performed using a real-time RT-PCR machine (model Mx4000; Multiplex Quantitative PCR System, Stratagene, La Jolla, CA), published oligonucleotide primers for MMP-2, MMP-7, and MMP-9 (Integrated DNA Technologies, Coralville, Iowa), and iQSYBR-Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results ( ). ..

Multiplex Assay:

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Increased vascular angiotensin type 2 receptor expression and NOS-mediated mechanisms of vascular relaxation in pregnant rats
Article Snippet: .. Quantification of gene expression was performed using a real-time quantitative RT-PCR machine (Mx4000, Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and using previously published oligonucleotide primers specific for eNOS, AT1 R, and AT2 R (Integrated DNA Technologies, Coralville, IA) and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR Green for amplicon detection (Bio-Rad, Hercules, CA). .. The α-actin primer with an expected product length of 285 bp was included in the RT-PCR as an internal standard to normalize the results.

Article Title: Increased MMPs expression and decreased contraction in the rat myometrium during pregnancy and in response to prolonged stretch and sex hormones
Article Snippet: .. Quantification of gene expression was performed using a real-time RT-PCR machine (model Mx4000; Multiplex Quantitative PCR System, Stratagene, La Jolla, CA), published oligonucleotide primers for MMP-2, MMP-7, and MMP-9 (Integrated DNA Technologies, Coralville, Iowa), and iQSYBR-Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results ( ). ..

Amplification:

Article Title: Diagnostic Performance of Schistosoma Real-Time PCR in Urine Samples from Kenyan Children Infected with Schistosoma haematobium: Day-to-day Variation and Follow-up after Praziquantel Treatment
Article Snippet: .. A CFX96 real-time PCR detection system (Bio-Rad) was used for amplification and amplicon detection, and CFX Manager version 1.6.514 (Bio-Rad) for related data analysis. .. The PCR output is expressed as Cyclic-threshold (Ct-) values.

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: No role of IFITM3 in brain tumor formation in vivo
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the house-keeping genes elongation factor 1α (Ef1α), β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase activation protein zeta (YHWAZ).

Article Title: Neurovascular EGFL7 regulates adult neurogenesis in the subventricular zone and thereby affects olfactory perception
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad, Hercules, CA, USA). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the housekeeping genes (HKG) glyceraldehyde-3-phosphate dehydrogenase , RNA polymerase II (RNAPII) and ribosomal protein S13 (Rps13).

Article Title: Increased MMPs expression and decreased contraction in the rat myometrium during pregnancy and in response to prolonged stretch and sex hormones
Article Snippet: .. Quantification of gene expression was performed using a real-time RT-PCR machine (model Mx4000; Multiplex Quantitative PCR System, Stratagene, La Jolla, CA), published oligonucleotide primers for MMP-2, MMP-7, and MMP-9 (Integrated DNA Technologies, Coralville, Iowa), and iQSYBR-Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results ( ). ..

IA:

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Increased vascular angiotensin type 2 receptor expression and NOS-mediated mechanisms of vascular relaxation in pregnant rats
Article Snippet: .. Quantification of gene expression was performed using a real-time quantitative RT-PCR machine (Mx4000, Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and using previously published oligonucleotide primers specific for eNOS, AT1 R, and AT2 R (Integrated DNA Technologies, Coralville, IA) and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR Green for amplicon detection (Bio-Rad, Hercules, CA). .. The α-actin primer with an expected product length of 285 bp was included in the RT-PCR as an internal standard to normalize the results.

Quantitative RT-PCR:

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Increased vascular angiotensin type 2 receptor expression and NOS-mediated mechanisms of vascular relaxation in pregnant rats
Article Snippet: .. Quantification of gene expression was performed using a real-time quantitative RT-PCR machine (Mx4000, Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and using previously published oligonucleotide primers specific for eNOS, AT1 R, and AT2 R (Integrated DNA Technologies, Coralville, IA) and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR Green for amplicon detection (Bio-Rad, Hercules, CA). .. The α-actin primer with an expected product length of 285 bp was included in the RT-PCR as an internal standard to normalize the results.

Article Title: Increased MMPs expression and decreased contraction in the rat myometrium during pregnancy and in response to prolonged stretch and sex hormones
Article Snippet: .. Quantification of gene expression was performed using a real-time RT-PCR machine (model Mx4000; Multiplex Quantitative PCR System, Stratagene, La Jolla, CA), published oligonucleotide primers for MMP-2, MMP-7, and MMP-9 (Integrated DNA Technologies, Coralville, Iowa), and iQSYBR-Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results ( ). ..

SYBR Green Assay:

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Increased vascular angiotensin type 2 receptor expression and NOS-mediated mechanisms of vascular relaxation in pregnant rats
Article Snippet: .. Quantification of gene expression was performed using a real-time quantitative RT-PCR machine (Mx4000, Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and using previously published oligonucleotide primers specific for eNOS, AT1 R, and AT2 R (Integrated DNA Technologies, Coralville, IA) and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR Green for amplicon detection (Bio-Rad, Hercules, CA). .. The α-actin primer with an expected product length of 285 bp was included in the RT-PCR as an internal standard to normalize the results.

Article Title: Increased MMPs expression and decreased contraction in the rat myometrium during pregnancy and in response to prolonged stretch and sex hormones
Article Snippet: .. Quantification of gene expression was performed using a real-time RT-PCR machine (model Mx4000; Multiplex Quantitative PCR System, Stratagene, La Jolla, CA), published oligonucleotide primers for MMP-2, MMP-7, and MMP-9 (Integrated DNA Technologies, Coralville, Iowa), and iQSYBR-Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results ( ). ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: No role of IFITM3 in brain tumor formation in vivo
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the house-keeping genes elongation factor 1α (Ef1α), β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase activation protein zeta (YHWAZ).

Article Title: Neurovascular EGFL7 regulates adult neurogenesis in the subventricular zone and thereby affects olfactory perception
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad, Hercules, CA, USA). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the housekeeping genes (HKG) glyceraldehyde-3-phosphate dehydrogenase , RNA polymerase II (RNAPII) and ribosomal protein S13 (Rps13).

Article Title: Increased MMPs expression and decreased contraction in the rat myometrium during pregnancy and in response to prolonged stretch and sex hormones
Article Snippet: .. Quantification of gene expression was performed using a real-time RT-PCR machine (model Mx4000; Multiplex Quantitative PCR System, Stratagene, La Jolla, CA), published oligonucleotide primers for MMP-2, MMP-7, and MMP-9 (Integrated DNA Technologies, Coralville, Iowa), and iQSYBR-Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results ( ). ..

Expressing:

Article Title: Gender-Specific Reduction in Contraction is Associated with Increased Estrogen Receptor Expression in Single Vascular Smooth Muscle Cells of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα and ERβ (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer with expected product length of 285 base pairs was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Adaptive Regulation of Endothelin Receptor Type A and Type B in Vascular Smooth Muscle Cells during Pregnancy in Rats
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ETA R and ETB R (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40–45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Subtype-Specific Estrogen Receptor-Mediated Vasodilator Activity in the Cephalic, Thoracic and Abdominal Vasculature of Female Rat
Article Snippet: .. Quantification of gene expression was performed using real-time quantitative RT-PCR machine (Mx4000 Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and employing published oligonucleotide primers specific for ERα, ERβ and GPR30 (Integrated DNA Technologies (IDT), Coralville, IA), and the Bio-Rad iQ SYBR Green Supermix for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results. .. PCR was carried out with 1 cycle for 10 min at 95°C then 40-45 cycles of 30 sec denaturation at 95°C, 45 sec of annealing at 56°C, and 30 sec of extension at 72°C, followed by 1 min of final extension step at 95°C.

Article Title: Increased vascular angiotensin type 2 receptor expression and NOS-mediated mechanisms of vascular relaxation in pregnant rats
Article Snippet: .. Quantification of gene expression was performed using a real-time quantitative RT-PCR machine (Mx4000, Multiplex Quantitative PCR System, Stratagene, La Jolla, CA) and using previously published oligonucleotide primers specific for eNOS, AT1 R, and AT2 R (Integrated DNA Technologies, Coralville, IA) and the Bio-Rad iQ SYBR Green Supermix, which employs the fluorescein compound SYBR Green for amplicon detection (Bio-Rad, Hercules, CA). .. The α-actin primer with an expected product length of 285 bp was included in the RT-PCR as an internal standard to normalize the results.

Article Title: Increased MMPs expression and decreased contraction in the rat myometrium during pregnancy and in response to prolonged stretch and sex hormones
Article Snippet: .. Quantification of gene expression was performed using a real-time RT-PCR machine (model Mx4000; Multiplex Quantitative PCR System, Stratagene, La Jolla, CA), published oligonucleotide primers for MMP-2, MMP-7, and MMP-9 (Integrated DNA Technologies, Coralville, Iowa), and iQSYBR-Green Supermix, which employs the fluorescein compound SYBR-Green for amplicon detection (Bio-Rad, Hercules, CA). α-Actin primer was included in the RT-PCR reaction as internal standard to normalize the results ( ). ..

Polymerase Chain Reaction:

Article Title: No role of IFITM3 in brain tumor formation in vivo
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the house-keeping genes elongation factor 1α (Ef1α), β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase activation protein zeta (YHWAZ).

Article Title: Neurovascular EGFL7 regulates adult neurogenesis in the subventricular zone and thereby affects olfactory perception
Article Snippet: .. PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad, Hercules, CA, USA). .. Quantification of expression was normalized according to the relative levels of cDNA in the samples based on quantitative analysis of the housekeeping genes (HKG) glyceraldehyde-3-phosphate dehydrogenase , RNA polymerase II (RNAPII) and ribosomal protein S13 (Rps13).

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    Bio-Rad amplicon detection
    Amplicon Detection, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 9 article reviews
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