Structured Review

ATUM ampicillin resistant pjexpress414
Ampicillin Resistant Pjexpress414, supplied by ATUM, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ampicillin resistant pjexpress414/product/ATUM
Average 86 stars, based on 2 article reviews
Price from $9.99 to $1999.99
ampicillin resistant pjexpress414 - by Bioz Stars, 2020-09
86/100 stars

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Construct:

Article Title: A multi-column plate adapter provides an economical and versatile high-throughput protein purification system
Article Snippet: .. SH3 domain and hybrid constructs were expressed from an ampicillin resistant pJexpress414 (DNA 2.0) plasmid to include an N-terminal Histidine-tag (his-tag) or from a pET21d (+) (Novagen) plasmid to include a C-terminal his-tag. contains additional information regarding individual samples. ..

Article Title: A semi-automated method for purification of milligram quantities of proteins on the QIAcube
Article Snippet: .. For the AbpSH3 construct, residues 535–592 of the yeast Abp1p protein (uniprot # ) were expressed from an ampicillin resistant pJexpress414 (DNA 2.0) plasmid to include a N-terminal histag (total size of protein 7800 Da). .. For the AbpSH3-ArkA construct, residues 535–592 of AbpSH3 were linked to KKTKPPVPPKPSHLKGT based on the yeast protein Ark1p (uniprot # C7GWN7 ) via a 17 amino acid linker GSGSENLYFQGGSYAMG and were expressed from a pJexpress414 plasmid (DNA 2.0) to include a N-terminal histag (total size 12, 400 Da).

Plasmid Preparation:

Article Title: A multi-column plate adapter provides an economical and versatile high-throughput protein purification system
Article Snippet: .. SH3 domain and hybrid constructs were expressed from an ampicillin resistant pJexpress414 (DNA 2.0) plasmid to include an N-terminal Histidine-tag (his-tag) or from a pET21d (+) (Novagen) plasmid to include a C-terminal his-tag. contains additional information regarding individual samples. ..

Article Title: A semi-automated method for purification of milligram quantities of proteins on the QIAcube
Article Snippet: .. For the AbpSH3 construct, residues 535–592 of the yeast Abp1p protein (uniprot # ) were expressed from an ampicillin resistant pJexpress414 (DNA 2.0) plasmid to include a N-terminal histag (total size of protein 7800 Da). .. For the AbpSH3-ArkA construct, residues 535–592 of AbpSH3 were linked to KKTKPPVPPKPSHLKGT based on the yeast protein Ark1p (uniprot # C7GWN7 ) via a 17 amino acid linker GSGSENLYFQGGSYAMG and were expressed from a pJexpress414 plasmid (DNA 2.0) to include a N-terminal histag (total size 12, 400 Da).