Structured Review

TaKaRa vector pmd18 t
Construction and analysis of resistance gene mutants. A. Schematic representation of homologous single recombination between a target resistance gene fragment cloned in <t>pMD18-T</t> and the target resistance gene in YNA11-2. Location of primers used for PCR is shown in the figure. The shaded box areas represent the target resistance gene while the lightly shaded area represents a cloned resistance gene fragment where homologous recombination occurred between pMD18-T and the genome of YNA11-2. When the mutant MaacA3 was analyzed, the primer accyan1-S, accyan1-A, accyan2-S and accyan2-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. When the mutant Marr3 was analyzed, the primer arryan2-S, arryan2-A, arryan4-S and arryan4-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. When the mutant MaadA1 was analyzed, the primer aadayan4-S, aadayan4-A, aadayan5-S and aadayan5-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. B. Electrophoresis of three resistance genes fragments and PCR identification of three mutants. M1, DNA markers; Lane 1–3, PCR products of three resistance genes fragments FaacA3, Farr3 and FaadA1; Lane 4–9, PCR products of MaacA3 with primer pairs p1 and p2, PCR products of Marr3 with primer pairs p1 and p2, PCR products of MaadA1 with primer pairs p1 and p2; M2, DNA markers.
Vector Pmd18 T, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 6015 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Identification and Characterization of Integron-Mediated Antibiotic Resistance in the Phytopathogen Xanthomonas oryzae pv. oryzae"

Article Title: Identification and Characterization of Integron-Mediated Antibiotic Resistance in the Phytopathogen Xanthomonas oryzae pv. oryzae

Journal: PLoS ONE

doi: 10.1371/journal.pone.0055962

Construction and analysis of resistance gene mutants. A. Schematic representation of homologous single recombination between a target resistance gene fragment cloned in pMD18-T and the target resistance gene in YNA11-2. Location of primers used for PCR is shown in the figure. The shaded box areas represent the target resistance gene while the lightly shaded area represents a cloned resistance gene fragment where homologous recombination occurred between pMD18-T and the genome of YNA11-2. When the mutant MaacA3 was analyzed, the primer accyan1-S, accyan1-A, accyan2-S and accyan2-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. When the mutant Marr3 was analyzed, the primer arryan2-S, arryan2-A, arryan4-S and arryan4-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. When the mutant MaadA1 was analyzed, the primer aadayan4-S, aadayan4-A, aadayan5-S and aadayan5-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. B. Electrophoresis of three resistance genes fragments and PCR identification of three mutants. M1, DNA markers; Lane 1–3, PCR products of three resistance genes fragments FaacA3, Farr3 and FaadA1; Lane 4–9, PCR products of MaacA3 with primer pairs p1 and p2, PCR products of Marr3 with primer pairs p1 and p2, PCR products of MaadA1 with primer pairs p1 and p2; M2, DNA markers.
Figure Legend Snippet: Construction and analysis of resistance gene mutants. A. Schematic representation of homologous single recombination between a target resistance gene fragment cloned in pMD18-T and the target resistance gene in YNA11-2. Location of primers used for PCR is shown in the figure. The shaded box areas represent the target resistance gene while the lightly shaded area represents a cloned resistance gene fragment where homologous recombination occurred between pMD18-T and the genome of YNA11-2. When the mutant MaacA3 was analyzed, the primer accyan1-S, accyan1-A, accyan2-S and accyan2-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. When the mutant Marr3 was analyzed, the primer arryan2-S, arryan2-A, arryan4-S and arryan4-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. When the mutant MaadA1 was analyzed, the primer aadayan4-S, aadayan4-A, aadayan5-S and aadayan5-A were used as the p1-S, p1-A, p2-S and p2-A, respectively. B. Electrophoresis of three resistance genes fragments and PCR identification of three mutants. M1, DNA markers; Lane 1–3, PCR products of three resistance genes fragments FaacA3, Farr3 and FaadA1; Lane 4–9, PCR products of MaacA3 with primer pairs p1 and p2, PCR products of Marr3 with primer pairs p1 and p2, PCR products of MaadA1 with primer pairs p1 and p2; M2, DNA markers.

Techniques Used: Clone Assay, Polymerase Chain Reaction, Homologous Recombination, Mutagenesis, Electrophoresis

2) Product Images from "Generation of Micronuclei during Interphase by Coupling between Cytoplasmic Membrane Blebbing and Nuclear Budding"

Article Title: Generation of Micronuclei during Interphase by Coupling between Cytoplasmic Membrane Blebbing and Nuclear Budding

Journal: PLoS ONE

doi: 10.1371/journal.pone.0027233

Time-lapse microscopy indicated that nuclear budding is coupled to cytoplasmic blebbing, which generates lamin-negative micronuclei. (A and B) The DNA in living COLO 320DM-GFP/lamin B1-mCherry cells was stained with Hoechst 33342 for time-lapse observation before and after fresh serum stimulation. The images were obtained at 3 min (A) or 5 min (B) intervals. The images corresponding to DM-GFP were omitted in A because the nuclear budding did not contain the DM-GFP signal. Elapsed time (in minutes:seconds) after the start of the experiment is shown in each image. White arrowheads indicate the cytoplasmic blebbing and nuclear budding.
Figure Legend Snippet: Time-lapse microscopy indicated that nuclear budding is coupled to cytoplasmic blebbing, which generates lamin-negative micronuclei. (A and B) The DNA in living COLO 320DM-GFP/lamin B1-mCherry cells was stained with Hoechst 33342 for time-lapse observation before and after fresh serum stimulation. The images were obtained at 3 min (A) or 5 min (B) intervals. The images corresponding to DM-GFP were omitted in A because the nuclear budding did not contain the DM-GFP signal. Elapsed time (in minutes:seconds) after the start of the experiment is shown in each image. White arrowheads indicate the cytoplasmic blebbing and nuclear budding.

Techniques Used: Time-lapse Microscopy, Staining

3) Product Images from "Signal Transducers and Activators of Transcription-3 Up-Regulates Tissue Inhibitor of Metalloproteinase-1 Expression and Decreases Invasiveness of Breast Cancer"

Article Title: Signal Transducers and Activators of Transcription-3 Up-Regulates Tissue Inhibitor of Metalloproteinase-1 Expression and Decreases Invasiveness of Breast Cancer

Journal:

doi: 10.2353/ajpath.2006.051109

TIMP1 expression correlated with invasiveness of MCF-7 cells. A: An increase in Matrigel invasion was observed in MCF-7 cells transfected with tet-off STAT3C and treated with increasing levels of tetracycline for a total of 48 hours. Cells treated with
Figure Legend Snippet: TIMP1 expression correlated with invasiveness of MCF-7 cells. A: An increase in Matrigel invasion was observed in MCF-7 cells transfected with tet-off STAT3C and treated with increasing levels of tetracycline for a total of 48 hours. Cells treated with

Techniques Used: Expressing, Transfection

A: Western blot analysis of STAT3, FLAG, TIMP1, cyclin D1, Bcl-2, and Bcl-xL expression in MCF-7 cells transfected with tTA-TRE-STAT3C and treated with increasing concentrations of tetracycline for 24 hours. There was a gradual decrease in STAT3 and FLAG
Figure Legend Snippet: A: Western blot analysis of STAT3, FLAG, TIMP1, cyclin D1, Bcl-2, and Bcl-xL expression in MCF-7 cells transfected with tTA-TRE-STAT3C and treated with increasing concentrations of tetracycline for 24 hours. There was a gradual decrease in STAT3 and FLAG

Techniques Used: Western Blot, Expressing, Transfection

TIMP1 levels in supernatants evaluated by ELISA. TIMP1 levels were measured using an ELISA assay in MCF-7 cells transfected with tTA-TRE-STAT3C and treated with 60 ng/ml of tetracycline. Supernatants from the tissue culture were collected on days 1, 2,
Figure Legend Snippet: TIMP1 levels in supernatants evaluated by ELISA. TIMP1 levels were measured using an ELISA assay in MCF-7 cells transfected with tTA-TRE-STAT3C and treated with 60 ng/ml of tetracycline. Supernatants from the tissue culture were collected on days 1, 2,

Techniques Used: Enzyme-linked Immunosorbent Assay, Transfection

4) Product Images from "A robust TALENs system for highly efficient mammalian genome editing"

Article Title: A robust TALENs system for highly efficient mammalian genome editing

Journal: Scientific Reports

doi: 10.1038/srep03632

Enrichment of SKOV3 cells transfected with both TALEN constructs. (A) Schematic view of TALENs that target human miR-210. The highlighted region indicates 22 nucleotides of mature miR-210 sequence. (B) Top panel, transfected SKOV3 cells before FACS. Approximately 50% and 20% of cells were transfected with a TALEN-L construct encoding EGFP and a TALEN-R construct encoding DsRed, respectively. Bottom panel, SKOV3 cells plated right after FACS. All cells are labelled by both EGFP and DsRed. Size of the bar, 100 μm. (C) Surveyor assay of DNA extracted from SKOV3 cells before and after FACS. The numbers at the bottom of the gel indicate mutation percentages measured by band intensities. Genomic editing is enhanced 3.8-fold following cell sorting.
Figure Legend Snippet: Enrichment of SKOV3 cells transfected with both TALEN constructs. (A) Schematic view of TALENs that target human miR-210. The highlighted region indicates 22 nucleotides of mature miR-210 sequence. (B) Top panel, transfected SKOV3 cells before FACS. Approximately 50% and 20% of cells were transfected with a TALEN-L construct encoding EGFP and a TALEN-R construct encoding DsRed, respectively. Bottom panel, SKOV3 cells plated right after FACS. All cells are labelled by both EGFP and DsRed. Size of the bar, 100 μm. (C) Surveyor assay of DNA extracted from SKOV3 cells before and after FACS. The numbers at the bottom of the gel indicate mutation percentages measured by band intensities. Genomic editing is enhanced 3.8-fold following cell sorting.

Techniques Used: Transfection, Construct, TALENs, Sequencing, FACS, Mutagenesis

Our improved TALENs system. (A) Schematic view of the TALEN structure with the DNA-binding modules highlighted. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = T, HD = C, NI = A, and NN = G or A. (B) The TALENs system we have improved. A widely-used plasmid backbone, pcDNA3.1(−), was chosen for the TALEN vector. The red asterisks indicate where the BsaI sites were in the original pcDNA3.1(−) plasmid. They have been destroyed by mutagenesis. The original neomycin-resistant gene was replaced by a gene encoding EGFP or DsRed. The TALEN coding sequence and an adjacent upstream T7 promoter can be isolated by digesting the construct with SacI and PmeI (in red) as a template for in vitro transcription of TALEN mRNAs.
Figure Legend Snippet: Our improved TALENs system. (A) Schematic view of the TALEN structure with the DNA-binding modules highlighted. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = T, HD = C, NI = A, and NN = G or A. (B) The TALENs system we have improved. A widely-used plasmid backbone, pcDNA3.1(−), was chosen for the TALEN vector. The red asterisks indicate where the BsaI sites were in the original pcDNA3.1(−) plasmid. They have been destroyed by mutagenesis. The original neomycin-resistant gene was replaced by a gene encoding EGFP or DsRed. The TALEN coding sequence and an adjacent upstream T7 promoter can be isolated by digesting the construct with SacI and PmeI (in red) as a template for in vitro transcription of TALEN mRNAs.

Techniques Used: TALENs, Binding Assay, Plasmid Preparation, Mutagenesis, Sequencing, Isolation, Construct, In Vitro

5) Product Images from "A robust TALENs system for highly efficient mammalian genome editing"

Article Title: A robust TALENs system for highly efficient mammalian genome editing

Journal: Scientific Reports

doi: 10.1038/srep03632

Enrichment of SKOV3 cells transfected with both TALEN constructs. (A) Schematic view of TALENs that target human miR-210. The highlighted region indicates 22 nucleotides of mature miR-210 sequence. (B) Top panel, transfected SKOV3 cells before FACS. Approximately 50% and 20% of cells were transfected with a TALEN-L construct encoding EGFP and a TALEN-R construct encoding DsRed, respectively. Bottom panel, SKOV3 cells plated right after FACS. All cells are labelled by both EGFP and DsRed. Size of the bar, 100 μm. (C) Surveyor assay of DNA extracted from SKOV3 cells before and after FACS. The numbers at the bottom of the gel indicate mutation percentages measured by band intensities. Genomic editing is enhanced 3.8-fold following cell sorting.
Figure Legend Snippet: Enrichment of SKOV3 cells transfected with both TALEN constructs. (A) Schematic view of TALENs that target human miR-210. The highlighted region indicates 22 nucleotides of mature miR-210 sequence. (B) Top panel, transfected SKOV3 cells before FACS. Approximately 50% and 20% of cells were transfected with a TALEN-L construct encoding EGFP and a TALEN-R construct encoding DsRed, respectively. Bottom panel, SKOV3 cells plated right after FACS. All cells are labelled by both EGFP and DsRed. Size of the bar, 100 μm. (C) Surveyor assay of DNA extracted from SKOV3 cells before and after FACS. The numbers at the bottom of the gel indicate mutation percentages measured by band intensities. Genomic editing is enhanced 3.8-fold following cell sorting.

Techniques Used: Transfection, Construct, TALENs, Sequencing, FACS, Mutagenesis

Our improved TALENs system. (A) Schematic view of the TALEN structure with the DNA-binding modules highlighted. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = T, HD = C, NI = A, and NN = G or A. (B) The TALENs system we have improved. A widely-used plasmid backbone, pcDNA3.1(−), was chosen for the TALEN vector. The red asterisks indicate where the BsaI sites were in the original pcDNA3.1(−) plasmid. They have been destroyed by mutagenesis. The original neomycin-resistant gene was replaced by a gene encoding EGFP or DsRed. The TALEN coding sequence and an adjacent upstream T7 promoter can be isolated by digesting the construct with SacI and PmeI (in red) as a template for in vitro transcription of TALEN mRNAs.
Figure Legend Snippet: Our improved TALENs system. (A) Schematic view of the TALEN structure with the DNA-binding modules highlighted. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = T, HD = C, NI = A, and NN = G or A. (B) The TALENs system we have improved. A widely-used plasmid backbone, pcDNA3.1(−), was chosen for the TALEN vector. The red asterisks indicate where the BsaI sites were in the original pcDNA3.1(−) plasmid. They have been destroyed by mutagenesis. The original neomycin-resistant gene was replaced by a gene encoding EGFP or DsRed. The TALEN coding sequence and an adjacent upstream T7 promoter can be isolated by digesting the construct with SacI and PmeI (in red) as a template for in vitro transcription of TALEN mRNAs.

Techniques Used: TALENs, Binding Assay, Plasmid Preparation, Mutagenesis, Sequencing, Isolation, Construct, In Vitro

6) Product Images from "Generation of Micronuclei during Interphase by Coupling between Cytoplasmic Membrane Blebbing and Nuclear Budding"

Article Title: Generation of Micronuclei during Interphase by Coupling between Cytoplasmic Membrane Blebbing and Nuclear Budding

Journal: PLoS ONE

doi: 10.1371/journal.pone.0027233

Time-lapse microscopy indicated that nuclear budding is coupled to cytoplasmic blebbing, which generates lamin-negative micronuclei. (A and B) The DNA in living COLO 320DM-GFP/lamin B1-mCherry cells was stained with Hoechst 33342 for time-lapse observation before and after fresh serum stimulation. The images were obtained at 3 min (A) or 5 min (B) intervals. The images corresponding to DM-GFP were omitted in A because the nuclear budding did not contain the DM-GFP signal. Elapsed time (in minutes:seconds) after the start of the experiment is shown in each image. White arrowheads indicate the cytoplasmic blebbing and nuclear budding.
Figure Legend Snippet: Time-lapse microscopy indicated that nuclear budding is coupled to cytoplasmic blebbing, which generates lamin-negative micronuclei. (A and B) The DNA in living COLO 320DM-GFP/lamin B1-mCherry cells was stained with Hoechst 33342 for time-lapse observation before and after fresh serum stimulation. The images were obtained at 3 min (A) or 5 min (B) intervals. The images corresponding to DM-GFP were omitted in A because the nuclear budding did not contain the DM-GFP signal. Elapsed time (in minutes:seconds) after the start of the experiment is shown in each image. White arrowheads indicate the cytoplasmic blebbing and nuclear budding.

Techniques Used: Time-lapse Microscopy, Staining

Related Articles

Amplification:

Article Title: Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer
Article Snippet: .. The results showed that the use of 0.1–0.15 U of high-fidelity DNA polymerase combined with 1 U of Taq DNA polymerase in per 20 μL reaction resulted in the best discrimination and amplification effects ( ). .. Thus, a mix of 0.1–0.15 U of high-fidelity DNA polymerase with 1 U of Taq DNA polymerase in per 20 μL reaction was selected for the subsequent experiments.

Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
Article Snippet: .. For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. We have used Pfu DNA polymerase in PCR amplification of RV sequence and found that this enzyme produced smaller amounts of DNA than TaKaRa Taq polymerase and also amplified DNA fragments over 3 kb poorly.

Isolation:

Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

Polymerase Chain Reaction:

Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
Article Snippet: .. PCR amplifications were performed in separate reactions containing cDNA, 1 pmol of primers, 0.4 mM deoxynucleoside triphosphates, 10% dimethyl sulfoxide, and proofreading TaKaRa Taq polymerase (Takara Shuzo Co., Ltd.) in a buffer provided by the manufacturer. .. The PCR program consisted of 1 min at 98°C followed by 25 cycles of 20 s at 98°C, 1 s at 56°C, and varied extension times (depending on the length of each sequence) at 70°C.

Article Title: Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence
Article Snippet: .. PCR-RFLP Both PCRs were performed in 25 μ L volume containing 2 μ L of the DNA sample, 0.2 μ L of Taq polymerase (TaKaRa, Dalian, China), 2.5 μ L of 10×Taq buffer (TaKaRa), 2 μ L of diethylnitrophenyl thiophosphate (dNTP, TaKaRa) mixture, 0.5 μ L of each primer (AF/AR or CAF/CAR, 50 mM), and 17.3 μ L of distilled water. .. PCR cycling parameters were as follows: 1 cycle at 96°C for 5 minutes, then 35 cycles of 96°C for 30 seconds, at 60°C for 30 seconds, and at 72°C for 90 seconds, followed by 1 cycle at 72°C for 7 minutes.

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: .. The polymerase chain reaction (PCR) reaction was 25 μL containing 100 ng templates, 0.5 μM of each primer, 0.5 mM dNTPs, 2.0 U Taq DNA polymerase and 2.5 μL buffer (TaKaRa, Tokyo, Japan). ..

other:

Article Title: Thermostable DNA Ligase-Mediated PCR Production of Circular Plasmid (PPCP) and Its Application in Directed Evolution via In situ Error-Prone PCR
Article Snippet: Error-prone PPCP using Taq DNA polymerase in the presence of Mn2+ , Tma DNA ligase and the PPCP primer pair from pHsh-kan was performed over the xynA 1 region of pHsh-xynA 1 template (Fig. ).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
Article Snippet: .. For the construction of a full-length RV M33 cDNA clone, six overlapping DNA fragments covering the entire viral genome were individually amplified by RT-PCR with TaKaRa Taq polymerase, which has been used successfully for construction of the infectious cDNA clone of wild-type RV (f-Therien strain) ( ). .. We have used Pfu DNA polymerase in PCR amplification of RV sequence and found that this enzyme produced smaller amounts of DNA than TaKaRa Taq polymerase and also amplified DNA fragments over 3 kb poorly.

Chloramphenicol Acetyltransferase Assay:

Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

Activated Clotting Time Assay:

Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain
Article Snippet: .. PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′). .. Size of the amplification product was estimated by gel electrophoresis for genotyping (210 bp for wild-type and 350 bp for GluR2 delta7; 440 bp for wild-type and 300 bp for GluR3 delta7).

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    TaKaRa ampicillin resistant gene
    Ampicillin Resistant Gene, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin resistant gene/product/TaKaRa
    Average 88 stars, based on 1 article reviews
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