mtra coding sequence  (Roche)


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    Structured Review

    Roche mtra coding sequence
    Interactions of <t>MtrA</t> with the upstream regions of bldD and whiI. (A) For EMSAs, a fixed amount of labeled probe containing the intergenic region upstream of the indicated gene was incubated in reactions containing no MtrA (lane 1), or 1.6 or 3.2 μg MtrA (lanes 2 and 3, respectively). The <t>DNA</t> fragments used as probes were amplified by PCR. The length of the probe for each upstream region is: bldD (282 bp), bldKA (265 bp), whiE -ORF1 (267 bp), and whiI (293 bp). The arrow indicates the position of free probes, and arrowheads indicate the position of the shifted probes. DNase I footprinting assays of the coding strand of bldD (B) and whiI (C) . The upper electropherograms in red are the control reactions without addition of MtrA protein. The reactions including MtrA are shown below in blue. The MtrA-protected regions are indicated by the dotted rectangles. The superimposed electropherograms shows the sequencing reactions. The nucleotide sequence of the MtrA-protected region for each gene is marked by bold italics and a double arrow, and the numbers indicate position relative to the TSP of each gene. The potential MtrA sequence is underlined.
    Mtra Coding Sequence, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 12724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtra coding sequence/product/Roche
    Average 91 stars, based on 12724 article reviews
    Price from $9.99 to $1999.99
    mtra coding sequence - by Bioz Stars, 2020-09
    91/100 stars

    Images

    1) Product Images from "Deletion of MtrA Inhibits Cellular Development of Streptomyces coelicolor and Alters Expression of Developmental Regulatory Genes"

    Article Title: Deletion of MtrA Inhibits Cellular Development of Streptomyces coelicolor and Alters Expression of Developmental Regulatory Genes

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.02013

    Interactions of MtrA with the upstream regions of bldD and whiI. (A) For EMSAs, a fixed amount of labeled probe containing the intergenic region upstream of the indicated gene was incubated in reactions containing no MtrA (lane 1), or 1.6 or 3.2 μg MtrA (lanes 2 and 3, respectively). The DNA fragments used as probes were amplified by PCR. The length of the probe for each upstream region is: bldD (282 bp), bldKA (265 bp), whiE -ORF1 (267 bp), and whiI (293 bp). The arrow indicates the position of free probes, and arrowheads indicate the position of the shifted probes. DNase I footprinting assays of the coding strand of bldD (B) and whiI (C) . The upper electropherograms in red are the control reactions without addition of MtrA protein. The reactions including MtrA are shown below in blue. The MtrA-protected regions are indicated by the dotted rectangles. The superimposed electropherograms shows the sequencing reactions. The nucleotide sequence of the MtrA-protected region for each gene is marked by bold italics and a double arrow, and the numbers indicate position relative to the TSP of each gene. The potential MtrA sequence is underlined.
    Figure Legend Snippet: Interactions of MtrA with the upstream regions of bldD and whiI. (A) For EMSAs, a fixed amount of labeled probe containing the intergenic region upstream of the indicated gene was incubated in reactions containing no MtrA (lane 1), or 1.6 or 3.2 μg MtrA (lanes 2 and 3, respectively). The DNA fragments used as probes were amplified by PCR. The length of the probe for each upstream region is: bldD (282 bp), bldKA (265 bp), whiE -ORF1 (267 bp), and whiI (293 bp). The arrow indicates the position of free probes, and arrowheads indicate the position of the shifted probes. DNase I footprinting assays of the coding strand of bldD (B) and whiI (C) . The upper electropherograms in red are the control reactions without addition of MtrA protein. The reactions including MtrA are shown below in blue. The MtrA-protected regions are indicated by the dotted rectangles. The superimposed electropherograms shows the sequencing reactions. The nucleotide sequence of the MtrA-protected region for each gene is marked by bold italics and a double arrow, and the numbers indicate position relative to the TSP of each gene. The potential MtrA sequence is underlined.

    Techniques Used: Labeling, Incubation, Amplification, Polymerase Chain Reaction, Footprinting, Sequencing

    Related Articles

    Amplification:

    Article Title: WHIRLY1 is a major organizer of chloroplast nucleoids
    Article Snippet: .. The amplified fragments specific for either nuclear 18S rDNA or plastid petD were used as templates for DIG-DNA labeling (digoxigenin) using a kit (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche Applied Science, Mannheim, Germany) according to the manufacturer's protocol. .. For q-PCR analyses a QuantiFast SYBR Green PCR Kit (Qiagen, Hilden, Germany) was used according to the manufacturer's protocol using gene specific primers (Supplementary Table ).

    Immunodetection:

    Article Title: Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator
    Article Snippet: .. Labeling of the PCR product with DIG as well as hybridization of the probe to the blot and immunodetection was performed with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche) according to the manufacturer's recommendations. .. First strand cDNA-synthesis Total RNA from G. stearothermophilus was isolated with the High Pure RNA Isolation Kit (Roche).

    Labeling:

    Article Title: Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator
    Article Snippet: .. Labeling of the PCR product with DIG as well as hybridization of the probe to the blot and immunodetection was performed with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche) according to the manufacturer's recommendations. .. First strand cDNA-synthesis Total RNA from G. stearothermophilus was isolated with the High Pure RNA Isolation Kit (Roche).

    Article Title: Identification and molecular characterization of a ?-1,4-endoglucanase gene (Rr-eng-1) from Rotylenchulus reniformis
    Article Snippet: .. DIG (digoxigenin-11-dUTP) probe labeling, prehybridization, hybridization (48°C), blot washing, and immunological detection was performed in accordance with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Applied Science, Mannheim, Germany). .. Quantitative real-time RT-PCR (qRT-PCR): First-strand cDNA was reverse-transcribed from approximately 500 ng of DNase-treated total RNA from each R . reniformis life-stage using the SuperScript™ First-Strand Synthesis System for RT-PCR (Invitrogen, CA) per the manufacturer's instructions with the following modification: In addition to 0.5 μg of an oligo(dT)12-18 primer, each 20 μL reaction contained 0.1 μM of a R . reniformis 18S-specific primer (5′-AACCAGGGCGCTCATTGAGTCTTA-3′) to facilitate normalization of Rr-eng-1 expression levels.

    Article Title: Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae
    Article Snippet: .. Probe labeling, hybridization (42 °C), and signal detection were performed using DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Germany) according to the protocols of the manufacturer. .. RNA isolation and reverse transcription (RT) PCR Total RNA was isolated from exponentially growing cultures of C. vulgaris using TotalRNAExtractor Reagent (Sangon Biotech, China) according to the protocols of the manufacturer.

    Article Title: WHIRLY1 is a major organizer of chloroplast nucleoids
    Article Snippet: .. The amplified fragments specific for either nuclear 18S rDNA or plastid petD were used as templates for DIG-DNA labeling (digoxigenin) using a kit (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche Applied Science, Mannheim, Germany) according to the manufacturer's protocol. .. For q-PCR analyses a QuantiFast SYBR Green PCR Kit (Qiagen, Hilden, Germany) was used according to the manufacturer's protocol using gene specific primers (Supplementary Table ).

    Article Title: Contribution of the Caspase Gene Sequence Diversification to the Specifically Antiviral Defense in Invertebrate
    Article Snippet: .. The DIG labeling and detection were performed following the protocol of the DIG High Prime DNA Labeling and Detection Starter Kit (Roche, Grenzacherstrasse, Basel, Switzerland). .. Synthesis of siRNA The siRNAs used in this study consisted of 21-nucleotide double-stranded RNAs, each strand of which contained a 17∼22-nucleotide target sequence and a two-uracil (U) overhang at the 3′ end .

    DNA Labeling:

    Article Title: Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission
    Article Snippet: .. The membrane was analyzed with DIG-labeled 3D8 scFv probes (25 ng/ml) using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche). .. 3D8 scFv gene expression in transgenic chickens To analyze the 3D8 scFv mRNA expression patterns in transgenic chickens, tissue samples were harvested and homogenized using TRIzol and a PureLink® RNA Mini Kit (Invitrogen) according to the manufacturer’s protocol.

    Article Title: Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator
    Article Snippet: .. Labeling of the PCR product with DIG as well as hybridization of the probe to the blot and immunodetection was performed with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche) according to the manufacturer's recommendations. .. First strand cDNA-synthesis Total RNA from G. stearothermophilus was isolated with the High Pure RNA Isolation Kit (Roche).

    Article Title: Identification and molecular characterization of a ?-1,4-endoglucanase gene (Rr-eng-1) from Rotylenchulus reniformis
    Article Snippet: .. DIG (digoxigenin-11-dUTP) probe labeling, prehybridization, hybridization (48°C), blot washing, and immunological detection was performed in accordance with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Applied Science, Mannheim, Germany). .. Quantitative real-time RT-PCR (qRT-PCR): First-strand cDNA was reverse-transcribed from approximately 500 ng of DNase-treated total RNA from each R . reniformis life-stage using the SuperScript™ First-Strand Synthesis System for RT-PCR (Invitrogen, CA) per the manufacturer's instructions with the following modification: In addition to 0.5 μg of an oligo(dT)12-18 primer, each 20 μL reaction contained 0.1 μM of a R . reniformis 18S-specific primer (5′-AACCAGGGCGCTCATTGAGTCTTA-3′) to facilitate normalization of Rr-eng-1 expression levels.

    Article Title: Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae
    Article Snippet: .. Probe labeling, hybridization (42 °C), and signal detection were performed using DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Germany) according to the protocols of the manufacturer. .. RNA isolation and reverse transcription (RT) PCR Total RNA was isolated from exponentially growing cultures of C. vulgaris using TotalRNAExtractor Reagent (Sangon Biotech, China) according to the protocols of the manufacturer.

    Article Title: Genetic Removal of the CH1 Exon Enables the Production of Heavy Chain-Only IgG in Mice
    Article Snippet: .. Hybridization and detection were performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche, Basel, Switzerland), according to the manufacturer's instructions. .. Positive ES cell clones were subsequently microinjected into the blastula of C57BL/6J mouse using standard protocols.

    Article Title: WHIRLY1 is a major organizer of chloroplast nucleoids
    Article Snippet: .. The amplified fragments specific for either nuclear 18S rDNA or plastid petD were used as templates for DIG-DNA labeling (digoxigenin) using a kit (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche Applied Science, Mannheim, Germany) according to the manufacturer's protocol. .. For q-PCR analyses a QuantiFast SYBR Green PCR Kit (Qiagen, Hilden, Germany) was used according to the manufacturer's protocol using gene specific primers (Supplementary Table ).

    Article Title: Contribution of the Caspase Gene Sequence Diversification to the Specifically Antiviral Defense in Invertebrate
    Article Snippet: .. The DIG labeling and detection were performed following the protocol of the DIG High Prime DNA Labeling and Detection Starter Kit (Roche, Grenzacherstrasse, Basel, Switzerland). .. Synthesis of siRNA The siRNAs used in this study consisted of 21-nucleotide double-stranded RNAs, each strand of which contained a 17∼22-nucleotide target sequence and a two-uracil (U) overhang at the 3′ end .

    Article Title: Native cell-death genes as candidates for developing wilt resistance in transgenic banana plants
    Article Snippet: .. Chemiluminescent detection of hybridization signals was subsequently carried out in a chemiluminescence detector enabled gel documentation system using DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Science, Germany) according to the manufacturer's instructions. .. Real-time quantitative RT–PCR In order to estimate the exact quantum of overexpression of the three cell-death-related genes in the respective transgenic banana lines, quantitative RT–PCRs were performed.

    Polymerase Chain Reaction:

    Article Title: Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator
    Article Snippet: .. Labeling of the PCR product with DIG as well as hybridization of the probe to the blot and immunodetection was performed with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche) according to the manufacturer's recommendations. .. First strand cDNA-synthesis Total RNA from G. stearothermophilus was isolated with the High Pure RNA Isolation Kit (Roche).

    Hybridization:

    Article Title: Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator
    Article Snippet: .. Labeling of the PCR product with DIG as well as hybridization of the probe to the blot and immunodetection was performed with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche) according to the manufacturer's recommendations. .. First strand cDNA-synthesis Total RNA from G. stearothermophilus was isolated with the High Pure RNA Isolation Kit (Roche).

    Article Title: Identification and molecular characterization of a ?-1,4-endoglucanase gene (Rr-eng-1) from Rotylenchulus reniformis
    Article Snippet: .. DIG (digoxigenin-11-dUTP) probe labeling, prehybridization, hybridization (48°C), blot washing, and immunological detection was performed in accordance with the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Applied Science, Mannheim, Germany). .. Quantitative real-time RT-PCR (qRT-PCR): First-strand cDNA was reverse-transcribed from approximately 500 ng of DNase-treated total RNA from each R . reniformis life-stage using the SuperScript™ First-Strand Synthesis System for RT-PCR (Invitrogen, CA) per the manufacturer's instructions with the following modification: In addition to 0.5 μg of an oligo(dT)12-18 primer, each 20 μL reaction contained 0.1 μM of a R . reniformis 18S-specific primer (5′-AACCAGGGCGCTCATTGAGTCTTA-3′) to facilitate normalization of Rr-eng-1 expression levels.

    Article Title: Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae
    Article Snippet: .. Probe labeling, hybridization (42 °C), and signal detection were performed using DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Germany) according to the protocols of the manufacturer. .. RNA isolation and reverse transcription (RT) PCR Total RNA was isolated from exponentially growing cultures of C. vulgaris using TotalRNAExtractor Reagent (Sangon Biotech, China) according to the protocols of the manufacturer.

    Article Title: Genetic Removal of the CH1 Exon Enables the Production of Heavy Chain-Only IgG in Mice
    Article Snippet: .. Hybridization and detection were performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche, Basel, Switzerland), according to the manufacturer's instructions. .. Positive ES cell clones were subsequently microinjected into the blastula of C57BL/6J mouse using standard protocols.

    Article Title: Native cell-death genes as candidates for developing wilt resistance in transgenic banana plants
    Article Snippet: .. Chemiluminescent detection of hybridization signals was subsequently carried out in a chemiluminescence detector enabled gel documentation system using DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Science, Germany) according to the manufacturer's instructions. .. Real-time quantitative RT–PCR In order to estimate the exact quantum of overexpression of the three cell-death-related genes in the respective transgenic banana lines, quantitative RT–PCRs were performed.

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    Roche ampicillin resistant dna sequence
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