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Promega ampicillin resistance genes
Ampicillin Resistance Genes, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ampicillin resistance genes/product/Promega
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ampicillin resistance genes - by Bioz Stars, 2020-09
88/100 stars

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Clone Assay:

Article Title: A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination
Article Snippet: .. Then LoxP-site coupled to ampicillin resistance gene was isolated from pT-FLAG plasmid by BspHI digestion and cloned into phRL-TK (Promega, Madison, WI, USA) or pBlueScriptII (Stratagene) digested by BspHI. ..

Article Title: Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells
Article Snippet: .. The ampicillin resistance gene was isolated by PCR from the plasmid pGEM-T (Promega, UK) by using an In-Fusion cloning kit (CloneTech, UK) and the primers PAR_AmpR For and Rev ( ). .. The resulting sticky-end PCR product contained an overhanging EagI site and was thereby ligated in a linearized PAR plasmid.

Luciferase:

Article Title: Percolation and Survival of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium in Soil Amended with Contaminated Dairy Manure or Slurry ▿
Article Snippet: .. The plasmid vector pBESTluc, containing an ampicillin resistance gene and the eukaryotic firefly luciferase ( luc ) gene positioned downstream from the tac promoter and a ribosome binding site, was obtained from Promega Corporation, Madison, WI (4,486 bp; E. coli S30 extract system for circular DNA). .. The pGFP cDNA vector (3,344 bp; Clontech Laboratories, Inc., Palo Alto, CA) contains the complete GFP coding sequence inserted in frame with the lacZ initiation codon from pUC19, a high-copy-number origin of replication, and an ampicillin resistance gene.

Ligation:

Article Title: Constitutive translation of human α-synuclein is mediated by the 5′-untranslated region
Article Snippet: .. The promoter-less empty and SNCA 5′-UTR psiCHECK2 reporters were constructed by partial double digestion of the psiCHECK2 plasmid with HindIII and BamHI restriction enzymes followed by ligation of the ampicillin resistance gene obtained from pGL3-basic (Promega) plasmid. ..

Isolation:

Article Title: A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination
Article Snippet: .. Then LoxP-site coupled to ampicillin resistance gene was isolated from pT-FLAG plasmid by BspHI digestion and cloned into phRL-TK (Promega, Madison, WI, USA) or pBlueScriptII (Stratagene) digested by BspHI. ..

Article Title: Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells
Article Snippet: .. The ampicillin resistance gene was isolated by PCR from the plasmid pGEM-T (Promega, UK) by using an In-Fusion cloning kit (CloneTech, UK) and the primers PAR_AmpR For and Rev ( ). .. The resulting sticky-end PCR product contained an overhanging EagI site and was thereby ligated in a linearized PAR plasmid.

Construct:

Article Title: Constitutive translation of human α-synuclein is mediated by the 5′-untranslated region
Article Snippet: .. The promoter-less empty and SNCA 5′-UTR psiCHECK2 reporters were constructed by partial double digestion of the psiCHECK2 plasmid with HindIII and BamHI restriction enzymes followed by ligation of the ampicillin resistance gene obtained from pGL3-basic (Promega) plasmid. ..

Article Title:
Article Snippet: .. The centromeric expression vector, pJHXSB (supplemental Fig. ), was constructed with a fragment containing the replication origin (ori) and an ampicillin resistance gene ( AmpR ) from pGEM13Zf (Promega Corp.), the URA3 gene fragment from YCp50 , and a fragment including CEN6 (CEN-ARS) from pRS317 (ATCC77157). .. The TPI1 promoter (TPI1p) and terminator (TPI1t) fragments were obtained from the chromosomal DNA of strain S288C by PCR using sets of oligonucleotides 9 + 10 and 11 + 12 as primers, respectively.

Expressing:

Article Title:
Article Snippet: .. The centromeric expression vector, pJHXSB (supplemental Fig. ), was constructed with a fragment containing the replication origin (ori) and an ampicillin resistance gene ( AmpR ) from pGEM13Zf (Promega Corp.), the URA3 gene fragment from YCp50 , and a fragment including CEN6 (CEN-ARS) from pRS317 (ATCC77157). .. The TPI1 promoter (TPI1p) and terminator (TPI1t) fragments were obtained from the chromosomal DNA of strain S288C by PCR using sets of oligonucleotides 9 + 10 and 11 + 12 as primers, respectively.

Polymerase Chain Reaction:

Article Title: Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells
Article Snippet: .. The ampicillin resistance gene was isolated by PCR from the plasmid pGEM-T (Promega, UK) by using an In-Fusion cloning kit (CloneTech, UK) and the primers PAR_AmpR For and Rev ( ). .. The resulting sticky-end PCR product contained an overhanging EagI site and was thereby ligated in a linearized PAR plasmid.

Transformation Assay:

Article Title: Structural Correlates of Antibacterial and Membrane-Permeabilizing Activities in Acylpolyamines
Article Snippet: .. Calcium chloride transformation of E. coli ML-35 was performed using the plasmid vector pBR322 , encoding tetracycline and ampicillin resistance genes (Promega, Madison, WI). .. The transformed strain, E. coli ML-35p, selected by ampicillin resistance, was utilized for the OM permeabilization assay.

Binding Assay:

Article Title: Percolation and Survival of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium in Soil Amended with Contaminated Dairy Manure or Slurry ▿
Article Snippet: .. The plasmid vector pBESTluc, containing an ampicillin resistance gene and the eukaryotic firefly luciferase ( luc ) gene positioned downstream from the tac promoter and a ribosome binding site, was obtained from Promega Corporation, Madison, WI (4,486 bp; E. coli S30 extract system for circular DNA). .. The pGFP cDNA vector (3,344 bp; Clontech Laboratories, Inc., Palo Alto, CA) contains the complete GFP coding sequence inserted in frame with the lacZ initiation codon from pUC19, a high-copy-number origin of replication, and an ampicillin resistance gene.

Plasmid Preparation:

Article Title: Constitutive translation of human α-synuclein is mediated by the 5′-untranslated region
Article Snippet: .. The promoter-less empty and SNCA 5′-UTR psiCHECK2 reporters were constructed by partial double digestion of the psiCHECK2 plasmid with HindIII and BamHI restriction enzymes followed by ligation of the ampicillin resistance gene obtained from pGL3-basic (Promega) plasmid. ..

Article Title: A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination
Article Snippet: .. Then LoxP-site coupled to ampicillin resistance gene was isolated from pT-FLAG plasmid by BspHI digestion and cloned into phRL-TK (Promega, Madison, WI, USA) or pBlueScriptII (Stratagene) digested by BspHI. ..

Article Title: Structural Correlates of Antibacterial and Membrane-Permeabilizing Activities in Acylpolyamines
Article Snippet: .. Calcium chloride transformation of E. coli ML-35 was performed using the plasmid vector pBR322 , encoding tetracycline and ampicillin resistance genes (Promega, Madison, WI). .. The transformed strain, E. coli ML-35p, selected by ampicillin resistance, was utilized for the OM permeabilization assay.

Article Title:
Article Snippet: .. The centromeric expression vector, pJHXSB (supplemental Fig. ), was constructed with a fragment containing the replication origin (ori) and an ampicillin resistance gene ( AmpR ) from pGEM13Zf (Promega Corp.), the URA3 gene fragment from YCp50 , and a fragment including CEN6 (CEN-ARS) from pRS317 (ATCC77157). .. The TPI1 promoter (TPI1p) and terminator (TPI1t) fragments were obtained from the chromosomal DNA of strain S288C by PCR using sets of oligonucleotides 9 + 10 and 11 + 12 as primers, respectively.

Article Title: Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells
Article Snippet: .. The ampicillin resistance gene was isolated by PCR from the plasmid pGEM-T (Promega, UK) by using an In-Fusion cloning kit (CloneTech, UK) and the primers PAR_AmpR For and Rev ( ). .. The resulting sticky-end PCR product contained an overhanging EagI site and was thereby ligated in a linearized PAR plasmid.

Article Title: Percolation and Survival of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium in Soil Amended with Contaminated Dairy Manure or Slurry ▿
Article Snippet: .. The plasmid vector pBESTluc, containing an ampicillin resistance gene and the eukaryotic firefly luciferase ( luc ) gene positioned downstream from the tac promoter and a ribosome binding site, was obtained from Promega Corporation, Madison, WI (4,486 bp; E. coli S30 extract system for circular DNA). .. The pGFP cDNA vector (3,344 bp; Clontech Laboratories, Inc., Palo Alto, CA) contains the complete GFP coding sequence inserted in frame with the lacZ initiation codon from pUC19, a high-copy-number origin of replication, and an ampicillin resistance gene.

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  • 90
    Promega ampicillin resistance gene
    Ampicillin Resistance Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin resistance gene/product/Promega
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ampicillin resistance gene - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    93
    Promega ampicillin resistance cassette
    Ampicillin Resistance Cassette, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin resistance cassette/product/Promega
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampicillin resistance cassette - by Bioz Stars, 2020-09
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      Buy from Supplier

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    Promega ampicillin resistance gene dsarg
    Ampicillin Resistance Gene Dsarg, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin resistance gene dsarg/product/Promega
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ampicillin resistance gene dsarg - by Bioz Stars, 2020-09
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      Buy from Supplier

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