Structured Review

PlasmidFactory ampicillin resistance gene
Ampicillin Resistance Gene, supplied by PlasmidFactory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ampicillin resistance gene/product/PlasmidFactory
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ampicillin resistance gene - by Bioz Stars, 2020-09
90/100 stars

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Related Articles

Cotransfection:

Article Title: Manufacturing and Characterization of a Recombinant Adeno-Associated Virus Type 8 Reference Standard Material
Article Snippet: .. Production was initiated by co-transfection of a certified master cell bank of HEK293 cells (ABG-EFS) in five-chamber Corning Cellstacks (at Nantes) or Corning Roller Bottles with plasmid pTR-UF-11 (ATCC # MBA-331), containing the vector genome and hGFP expression cassette (Burger et al. , ) and the pDP8 helper plasmid harboring the rep gene from AAV2, the cap gene from AAV8, the adenovirus helper genes E2A , E4 , and VA-RNA , and the ampicillin resistance gene (No. PF478; Plasmidfactory) using a calcium phosphate precipitation method. .. Same lots of FBS (Hyclone-Thermo scientific), trypsin (PAA Laboratories GmbH), DMEM (PAA), Pen/Strep (PAA), PBS (PAA), and dPBS (PAA) were split between the two manufacturing sites.

Plasmid Preparation:

Article Title: Manufacturing and Characterization of a Recombinant Adeno-Associated Virus Type 8 Reference Standard Material
Article Snippet: .. Production was initiated by co-transfection of a certified master cell bank of HEK293 cells (ABG-EFS) in five-chamber Corning Cellstacks (at Nantes) or Corning Roller Bottles with plasmid pTR-UF-11 (ATCC # MBA-331), containing the vector genome and hGFP expression cassette (Burger et al. , ) and the pDP8 helper plasmid harboring the rep gene from AAV2, the cap gene from AAV8, the adenovirus helper genes E2A , E4 , and VA-RNA , and the ampicillin resistance gene (No. PF478; Plasmidfactory) using a calcium phosphate precipitation method. .. Same lots of FBS (Hyclone-Thermo scientific), trypsin (PAA Laboratories GmbH), DMEM (PAA), Pen/Strep (PAA), PBS (PAA), and dPBS (PAA) were split between the two manufacturing sites.

Expressing:

Article Title: Manufacturing and Characterization of a Recombinant Adeno-Associated Virus Type 8 Reference Standard Material
Article Snippet: .. Production was initiated by co-transfection of a certified master cell bank of HEK293 cells (ABG-EFS) in five-chamber Corning Cellstacks (at Nantes) or Corning Roller Bottles with plasmid pTR-UF-11 (ATCC # MBA-331), containing the vector genome and hGFP expression cassette (Burger et al. , ) and the pDP8 helper plasmid harboring the rep gene from AAV2, the cap gene from AAV8, the adenovirus helper genes E2A , E4 , and VA-RNA , and the ampicillin resistance gene (No. PF478; Plasmidfactory) using a calcium phosphate precipitation method. .. Same lots of FBS (Hyclone-Thermo scientific), trypsin (PAA Laboratories GmbH), DMEM (PAA), Pen/Strep (PAA), PBS (PAA), and dPBS (PAA) were split between the two manufacturing sites.

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    PlasmidFactory ampicillin resistance gene
    Ampicillin Resistance Gene, supplied by PlasmidFactory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin resistance gene/product/PlasmidFactory
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampicillin resistance gene - by Bioz Stars, 2020-09
    90/100 stars
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