Structured Review

ATUM ampicillin resistance gene
Ampicillin Resistance Gene, supplied by ATUM, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ampicillin resistance gene/product/ATUM
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
ampicillin resistance gene - by Bioz Stars, 2020-09
93/100 stars

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Related Articles

Clone Assay:

Article Title: A novel thymidylate synthase from the Vibrionales, Alteromonadales, Aeromonadales, and Pasteurellales (VAAP) clade with altered nucleotide and folate binding sites
Article Snippet: .. VpTS expression and purification VpTS was expressed in Escherichia coli using a codon-optimized synthetic gene cloned into the T7-promoter expression vector pJexpress414 which contains the ampicillin resistance gene (DNA2.0). .. We used the amino acid sequence of Vibrio parahaemolyticus thymidylate synthase (GenBank WP_100088861.1 ) including in the N-terminus a 10 His-tag and the cutting site for the PreScission Protease (GE Healthcare, Little Chalfont, UK).

Article Title: A novel thymidylate synthase from the Vibrionales, Alteromonadales, Aeromonadales, and Pasteurellales (VAAP) clade with altered nucleotide and folate binding sites
Article Snippet: .. VpTS was expressed in Escherichia coli using a codon-optimized synthetic gene cloned into the T7-promoter expression vector pJexpress414 which contains the ampicillin resistance gene (DNA2.0). .. We used the amino acid sequence of Vibrio parahaemolyticus thymidylate synthase (GenBank ) including in the N-terminus a 10 His-tag and the cutting site for the PreScission Protease (GE Healthcare, Little Chalfont, UK).

Purification:

Article Title: A novel thymidylate synthase from the Vibrionales, Alteromonadales, Aeromonadales, and Pasteurellales (VAAP) clade with altered nucleotide and folate binding sites
Article Snippet: .. VpTS expression and purification VpTS was expressed in Escherichia coli using a codon-optimized synthetic gene cloned into the T7-promoter expression vector pJexpress414 which contains the ampicillin resistance gene (DNA2.0). .. We used the amino acid sequence of Vibrio parahaemolyticus thymidylate synthase (GenBank WP_100088861.1 ) including in the N-terminus a 10 His-tag and the cutting site for the PreScission Protease (GE Healthcare, Little Chalfont, UK).

Expressing:

Article Title: A novel thymidylate synthase from the Vibrionales, Alteromonadales, Aeromonadales, and Pasteurellales (VAAP) clade with altered nucleotide and folate binding sites
Article Snippet: .. VpTS expression and purification VpTS was expressed in Escherichia coli using a codon-optimized synthetic gene cloned into the T7-promoter expression vector pJexpress414 which contains the ampicillin resistance gene (DNA2.0). .. We used the amino acid sequence of Vibrio parahaemolyticus thymidylate synthase (GenBank WP_100088861.1 ) including in the N-terminus a 10 His-tag and the cutting site for the PreScission Protease (GE Healthcare, Little Chalfont, UK).

Article Title: A novel thymidylate synthase from the Vibrionales, Alteromonadales, Aeromonadales, and Pasteurellales (VAAP) clade with altered nucleotide and folate binding sites
Article Snippet: .. VpTS was expressed in Escherichia coli using a codon-optimized synthetic gene cloned into the T7-promoter expression vector pJexpress414 which contains the ampicillin resistance gene (DNA2.0). .. We used the amino acid sequence of Vibrio parahaemolyticus thymidylate synthase (GenBank ) including in the N-terminus a 10 His-tag and the cutting site for the PreScission Protease (GE Healthcare, Little Chalfont, UK).

Plasmid Preparation:

Article Title: A novel thymidylate synthase from the Vibrionales, Alteromonadales, Aeromonadales, and Pasteurellales (VAAP) clade with altered nucleotide and folate binding sites
Article Snippet: .. VpTS expression and purification VpTS was expressed in Escherichia coli using a codon-optimized synthetic gene cloned into the T7-promoter expression vector pJexpress414 which contains the ampicillin resistance gene (DNA2.0). .. We used the amino acid sequence of Vibrio parahaemolyticus thymidylate synthase (GenBank WP_100088861.1 ) including in the N-terminus a 10 His-tag and the cutting site for the PreScission Protease (GE Healthcare, Little Chalfont, UK).

Article Title: A novel thymidylate synthase from the Vibrionales, Alteromonadales, Aeromonadales, and Pasteurellales (VAAP) clade with altered nucleotide and folate binding sites
Article Snippet: .. VpTS was expressed in Escherichia coli using a codon-optimized synthetic gene cloned into the T7-promoter expression vector pJexpress414 which contains the ampicillin resistance gene (DNA2.0). .. We used the amino acid sequence of Vibrio parahaemolyticus thymidylate synthase (GenBank ) including in the N-terminus a 10 His-tag and the cutting site for the PreScission Protease (GE Healthcare, Little Chalfont, UK).

Synthesized:

Article Title: Conservation of Dynamics Associated with Biological Function in an Enzyme Superfamily
Article Snippet: .. Escherichia coli codon-optimized sequences of Pongo pygmaeus ribonuclease 3 (PpR3) and Macaca fascicularis ribonuclease 3 (MfR3) (UniProt entries and ) were synthesized in IPTG-inducible pJExpress414 vectors containing the ampicillin resistance gene (DNA2.0, Newark, CA). .. These vectors were transformed into E. coli BL21 (DE3) for all protein expression experiments.