ampicillin embedded luria bertani lb broth media  (Merck KGaA)

 
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    Structured Review

    Merck KGaA ampicillin embedded luria bertani lb broth media
    Ampicillin Embedded Luria Bertani Lb Broth Media, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin embedded luria bertani lb broth media/product/Merck KGaA
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampicillin embedded luria bertani lb broth media - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    mbp-ns3 protein
    ns3 insert

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    Related Articles

    Mutagenesis:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5. .. Then, protein expression was induced by adding isopropyl-β-D-thio-galactoside (IPTG) (Cinnagen, Tehran, Iran) at a final concentration of 1 mM.

    Cell Culture:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5. .. Then, protein expression was induced by adding isopropyl-β-D-thio-galactoside (IPTG) (Cinnagen, Tehran, Iran) at a final concentration of 1 mM.

    Purification:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: Expression and purification of MBP-NS3 fusion protein. .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5.

    Produced:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: Production of recombinant MBP-NS3 protein in pMalc2x expression vector, under the control of the lac promoter in E. coli BL-21 strain had been previously produced in our laboratory. .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5.

    Concentration Assay:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5. .. Then, protein expression was induced by adding isopropyl-β-D-thio-galactoside (IPTG) (Cinnagen, Tehran, Iran) at a final concentration of 1 mM.

    Incubation:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5. .. After 4 hr incubation at 37 ˚C, expression of the recombinant MBP-NS3 protein was examined by SDS-polyacrylamide gel electro-phoresis (SDS-PAGE).

    HAT Assay:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: Hypoxanthine aminopterin thymidine (HAT), hypoxanthine thymidine (HT), RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco Laboratories (Grand Island, USA). .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5.

    Expressing:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5. .. Then, protein expression was induced by adding isopropyl-β-D-thio-galactoside (IPTG) (Cinnagen, Tehran, Iran) at a final concentration of 1 mM.

    Western Blot:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5. .. To further analyze, expressed protein(s) were analyzed by Western blotting, using a BVDV antibody positive bovine serum (data not shown).

    Recombinant:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: Production of recombinant MBP-NS3 protein in pMalc2x expression vector, under the control of the lac promoter in E. coli BL-21 strain had been previously produced in our laboratory. .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5.

    SDS Page:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5. .. After 4 hr incubation at 37 ˚C, expression of the recombinant MBP-NS3 protein was examined by SDS-polyacrylamide gel electro-phoresis (SDS-PAGE).

    Plasmid Preparation:

    Article Title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
    Article Snippet: Production of recombinant MBP-NS3 protein in pMalc2x expression vector, under the control of the lac promoter in E. coli BL-21 strain had been previously produced in our laboratory. .. For expression of MBP-NS3 protein, a bacterial colony which had no mutation in the NS3 insert was selected and cultured in high volume of ampicillin embedded Luria-Bertani (LB) broth media (Merck, Darmstadt, Germany) containing 20 mM glucose, until the OD 600 reached to 0.5.

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  • 92
    Merck KGaA ampicillin embedded luria bertani lb broth media
    Ampicillin Embedded Luria Bertani Lb Broth Media, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin embedded luria bertani lb broth media/product/Merck KGaA
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampicillin embedded luria bertani lb broth media - by Bioz Stars, 2020-04
    92/100 stars
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