Structured Review

Spectrum Labs amicon
Amicon, supplied by Spectrum Labs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 87 stars, based on 1 article reviews
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amicon - by Bioz Stars, 2020-04
87/100 stars

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Related Articles

Clone Assay:

Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

Positron Emission Tomography:

Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

Chromatography:

Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

Expressing:

Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

Crystallization Assay:

Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

SDS Page:

Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

Plasmid Preparation:

Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

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