amicon protein concentrator  (Millipore)


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    Structured Review

    Millipore amicon protein concentrator
    Amicon Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon protein concentrator/product/Millipore
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amicon protein concentrator - by Bioz Stars, 2020-04
    92/100 stars

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    proteolysis mix
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    Related Articles

    Centrifugation:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: Sonication with a Bioruptor (Diagenode) was then used to lyse the cells, followed by centrifugation at 15,000 g for 1.5 h at 4°C. .. Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: After inducing for 16 h at room temperature, 250 ml of culture was harvested by centrifugation at 8,000 rpm for 8 min. Pellets were resuspended in a lysis buffer containing 50 mM NaH2 PO4 , 300 mM NaCl (pH 8.0), and 20 mM phenylmethylsulfonyl fluoride and sonicated to lyse the cells. .. The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma).

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The lysate supernatants were collected after centrifugation and the expressed GST-(Pro-Pro-Gly)5 was purified using glutathione affinity resin as described in “protein expression and purification” section of . .. After 2 h, the cleaved peptide was separated from GST by applying the proteolysis mix to a 10 kDa cut off Amicon protein concentrator (Millipore) and collecting the flow through.

    Electrophoresis:

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma). .. Proteins were then separated by SDS–10% PAGE electrophoresis, followed by Coomassie blue staining and destaining to estimate the concentration using a bovine serum albumin standard as reference.

    Incubation:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: After washing with 100 mL of the lysis buffer, incubation for 18 h at 4°C in a cleavage buffer (20 mM Tris-HCl, pH 8.5, 500 mM NaCl, 50 mM DTT, and 5 µM ZnSO4 ) was used to trigger on-column cleavage of the intein-CBD tag according to the instructions in the IMPACT kit (New England Biolabs). .. Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: The supernatant was collected and incubated for 1 h with 4 ml of 50% slurry of Ni FF resin (New England BioLabs) before being loading onto an empty EconoPac gravity-flow column (Bio-Rad Laboratories, Hercules, CA). .. The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma).

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: GST-(Pro-Pro-Gly)5 samples were then incubated with 50 U per mg-protein of thrombin to cleave GST tags from the (Pro-Pro-Gly)5 peptides at RT. .. After 2 h, the cleaved peptide was separated from GST by applying the proteolysis mix to a 10 kDa cut off Amicon protein concentrator (Millipore) and collecting the flow through.

    Activity Assay:

    Article Title: Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species ▿ Species ▿ †
    Article Snippet: Fractions containing olefin-forming activity, typically eluting between 600 and 750 mM NaCl, were pooled and dialyzed against buffer A. .. Fractions (1.5 ml) were collected, and active fractions, usually eluting between 150 and 200 mM NaCl, were pooled and concentrated using an Amicon protein concentrator (Millipore, Bedford, MA) with a 10-kDa exclusion size to about 50 μl.

    Expressing:

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: Once the cell density (i.e., the OD600 ) reached 0.5 to 0.7, 0.4 mM IPTG (isopropyl-β- d -thiogalactopyranoside; final concentration) was added to the culture to induce protein expression. .. The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma).

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The lysate supernatants were collected after centrifugation and the expressed GST-(Pro-Pro-Gly)5 was purified using glutathione affinity resin as described in “protein expression and purification” section of . .. After 2 h, the cleaved peptide was separated from GST by applying the proteolysis mix to a 10 kDa cut off Amicon protein concentrator (Millipore) and collecting the flow through.

    High Performance Liquid Chromatography:

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The peptides were then concentrated using a 3 kDa cut off Amicon protein concentrator (Millipore), heated at 95 °C for 5 min, and applied to a 0.2 μm spin filter microcon (Millipore) to remove possible residual protein impurities. .. The purity of the products was checked by SDS–PAGE and analytical HPLC (Waters), and the final peptide concentration was determined by measuring UV280 nm in Nanodrop spectrophotometer and analytical HPLC.

    Flow Cytometry:

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The proteins containing foldon were cleaved by Thrombin CleanCleave™ Kit (Sigma), and the cleaved products were separated from GST tag and uncleaved products by applying the mixture to glutathione affinity resin and collecting the flow through. .. The peptides were then concentrated using a 3 kDa cut off Amicon protein concentrator (Millipore), heated at 95 °C for 5 min, and applied to a 0.2 μm spin filter microcon (Millipore) to remove possible residual protein impurities.

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
    Article Snippet: Protein purification For enzyme purification from 2 L of IPTG-induced cells, Eco94GmrSD-6xHis was purified from fast flow nickel-NTA agarose columns (Qiagen). .. Active enzyme fractions were pooled and processed for buffer exchange by running through an Amicon protein concentrator (Millipore).

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: .. After 2 h, the cleaved peptide was separated from GST by applying the proteolysis mix to a 10 kDa cut off Amicon protein concentrator (Millipore) and collecting the flow through. .. The concentrations of the purified proteins were determined by UV280 nm with a Nanodrop spectrophotometer (Thermo Scientific).

    Chromatography:

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
    Article Snippet: The eluted fractions (5 ml × 6) were analyzed by SDS-PAGE and fractions containing GmrSD were further purified by chromatography through a 5 ml HiTrap heparin HP column (GE Life Sciences). .. Active enzyme fractions were pooled and processed for buffer exchange by running through an Amicon protein concentrator (Millipore).

    Article Title: Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species ▿ Species ▿ †
    Article Snippet: The dialyzed sample was then fractionated by anion exchange chromatography. .. Fractions (1.5 ml) were collected, and active fractions, usually eluting between 150 and 200 mM NaCl, were pooled and concentrated using an Amicon protein concentrator (Millipore, Bedford, MA) with a 10-kDa exclusion size to about 50 μl.

    Buffer Exchange:

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
    Article Snippet: .. Active enzyme fractions were pooled and processed for buffer exchange by running through an Amicon protein concentrator (Millipore). .. Protein was carefully recovered from the membrane by washing it a few times with a storage buffer (100 mM NaCl, 20 mM Tris-HCl, pH 7.5, 1 mM DTT, 50% glycerol) and the purified enzyme was stored at −20°C.

    Footprinting:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C. .. Proteins used for RNA footprinting assays were dialyzed against 10 mM Tris-HCl (pH 7.5), 20 mM KCl, 200 mM NaCl, 5 µM ZnSO4 , and 1 mM DTT and then concentrated to ∼50 µM as described above.

    Sonication:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: Sonication with a Bioruptor (Diagenode) was then used to lyse the cells, followed by centrifugation at 15,000 g for 1.5 h at 4°C. .. Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: After inducing for 16 h at room temperature, 250 ml of culture was harvested by centrifugation at 8,000 rpm for 8 min. Pellets were resuspended in a lysis buffer containing 50 mM NaH2 PO4 , 300 mM NaCl (pH 8.0), and 20 mM phenylmethylsulfonyl fluoride and sonicated to lyse the cells. .. The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma).

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: Cell pellets were collected, washed three times with DPBS, resuspended in DPBS, and then lysed by sonication. .. After 2 h, the cleaved peptide was separated from GST by applying the proteolysis mix to a 10 kDa cut off Amicon protein concentrator (Millipore) and collecting the flow through.

    Recombinant:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: Paragraph title: Recombinant Protein Purification ... Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species ▿ Species ▿ †
    Article Snippet: Fractions (1.5 ml) were collected, and active fractions, usually eluting between 150 and 200 mM NaCl, were pooled and concentrated using an Amicon protein concentrator (Millipore, Bedford, MA) with a 10-kDa exclusion size to about 50 μl. .. For purification of recombinant His-tagged proteins, E. coli was grown in LB medium supplemented with 100 μg/ml carbenicillin and 0.5 mM δ-aminolevulinic acid.

    Purification:

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: Paragraph title: Ribosomal protein L5 purification. ... The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma).

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
    Article Snippet: The eluted fractions (5 ml × 6) were analyzed by SDS-PAGE and fractions containing GmrSD were further purified by chromatography through a 5 ml HiTrap heparin HP column (GE Life Sciences). .. Active enzyme fractions were pooled and processed for buffer exchange by running through an Amicon protein concentrator (Millipore).

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The lysate supernatants were collected after centrifugation and the expressed GST-(Pro-Pro-Gly)5 was purified using glutathione affinity resin as described in “protein expression and purification” section of . .. After 2 h, the cleaved peptide was separated from GST by applying the proteolysis mix to a 10 kDa cut off Amicon protein concentrator (Millipore) and collecting the flow through.

    Article Title: Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species ▿ Species ▿ †
    Article Snippet: Fractions (1.5 ml) were collected, and active fractions, usually eluting between 150 and 200 mM NaCl, were pooled and concentrated using an Amicon protein concentrator (Millipore, Bedford, MA) with a 10-kDa exclusion size to about 50 μl. .. For purification of recombinant His-tagged proteins, E. coli was grown in LB medium supplemented with 100 μg/ml carbenicillin and 0.5 mM δ-aminolevulinic acid.

    Protein Purification:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: Paragraph title: Recombinant Protein Purification ... Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
    Article Snippet: Paragraph title: Protein purification ... Active enzyme fractions were pooled and processed for buffer exchange by running through an Amicon protein concentrator (Millipore).

    Article Title: Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species ▿ Species ▿ †
    Article Snippet: Paragraph title: Protein purification methods. ... Fractions (1.5 ml) were collected, and active fractions, usually eluting between 150 and 200 mM NaCl, were pooled and concentrated using an Amicon protein concentrator (Millipore, Bedford, MA) with a 10-kDa exclusion size to about 50 μl.

    Fast Protein Liquid Chromatography:

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
    Article Snippet: Pooled protein fractions were diluted in a low salt buffer (20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM DTT, 1 mM EDTA, 20 mM NaCl, 5% glycerol) and loaded onto a heparin HP column using an AKTA FPLC system (GE Life Sciences). .. Active enzyme fractions were pooled and processed for buffer exchange by running through an Amicon protein concentrator (Millipore).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma). .. Proteins were then separated by SDS–10% PAGE electrophoresis, followed by Coomassie blue staining and destaining to estimate the concentration using a bovine serum albumin standard as reference.

    Lysis:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: After washing with 100 mL of the lysis buffer, incubation for 18 h at 4°C in a cleavage buffer (20 mM Tris-HCl, pH 8.5, 500 mM NaCl, 50 mM DTT, and 5 µM ZnSO4 ) was used to trigger on-column cleavage of the intein-CBD tag according to the instructions in the IMPACT kit (New England Biolabs). .. Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: After inducing for 16 h at room temperature, 250 ml of culture was harvested by centrifugation at 8,000 rpm for 8 min. Pellets were resuspended in a lysis buffer containing 50 mM NaH2 PO4 , 300 mM NaCl (pH 8.0), and 20 mM phenylmethylsulfonyl fluoride and sonicated to lyse the cells. .. The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma).

    SDS Page:

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The peptides were then concentrated using a 3 kDa cut off Amicon protein concentrator (Millipore), heated at 95 °C for 5 min, and applied to a 0.2 μm spin filter microcon (Millipore) to remove possible residual protein impurities. .. The purity of the products was checked by SDS–PAGE and analytical HPLC (Waters), and the final peptide concentration was determined by measuring UV280 nm in Nanodrop spectrophotometer and analytical HPLC.

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: Protein concentrations were estimated by Coomassie Brilliant Blue staining of an SDS-PAGE gel using BSA of known concentrations as reference standards. .. Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
    Article Snippet: The eluted fractions corresponding to UV absorption peaks were analyzed by SDS-PAGE. .. Active enzyme fractions were pooled and processed for buffer exchange by running through an Amicon protein concentrator (Millipore).

    Spectrophotometry:

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The peptides were then concentrated using a 3 kDa cut off Amicon protein concentrator (Millipore), heated at 95 °C for 5 min, and applied to a 0.2 μm spin filter microcon (Millipore) to remove possible residual protein impurities. .. The purity of the products was checked by SDS–PAGE and analytical HPLC (Waters), and the final peptide concentration was determined by measuring UV280 nm in Nanodrop spectrophotometer and analytical HPLC.

    Concentration Assay:

    Article Title: Tunable, post-translational hydroxylation of collagen domains in Escherichia coli
    Article Snippet: The peptides were then concentrated using a 3 kDa cut off Amicon protein concentrator (Millipore), heated at 95 °C for 5 min, and applied to a 0.2 μm spin filter microcon (Millipore) to remove possible residual protein impurities. .. The purity of the products was checked by SDS–PAGE and analytical HPLC (Waters), and the final peptide concentration was determined by measuring UV280 nm in Nanodrop spectrophotometer and analytical HPLC.

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: Once the cell density (i.e., the OD600 ) reached 0.5 to 0.7, 0.4 mM IPTG (isopropyl-β- d -thiogalactopyranoside; final concentration) was added to the culture to induce protein expression. .. The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma).

    Staining:

    Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
    Article Snippet: Protein concentrations were estimated by Coomassie Brilliant Blue staining of an SDS-PAGE gel using BSA of known concentrations as reference standards. .. Proteins were concentrated with an Amicon protein concentrator (EMD Millipore) to ∼20 µM before being stored in 50-μL aliquots at −80°C.

    Article Title: Potato Spindle Tuber Viroid Modulates Its Replication through a Direct Interaction with a Splicing Regulator
    Article Snippet: The protein was then released with elution buffer (50 mM NaH2 PO4 , 300 mM NaCl, 400 mM imidazole) and concentrated using an Amicon protein concentrator (MilliporeSigma). .. Proteins were then separated by SDS–10% PAGE electrophoresis, followed by Coomassie blue staining and destaining to estimate the concentration using a bovine serum albumin standard as reference.

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  • 86
    Millipore dalton mw cutoff
    Dalton Mw Cutoff, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore amicon protein concentrator
    Amicon Protein Concentrator, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon protein concentrator/product/Millipore
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    92/100 stars
      Buy from Supplier

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