ambion dnase i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ambion dnase i
    Ambion Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ambion dnase i/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ambion dnase i - by Bioz Stars, 2020-04
    97/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Calcium-sensing receptor gene (CASR) polymorphisms and CASR transcript level concerning dyslipidemia in hemodialysis patients: a cross-sectional study
    Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (qPCR) analysis ... RNA samples were treated with Ambion DNase I (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1.5 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using an M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.

    Article Title: Atypical Microglial Response to Biodiesel Exhaust in Healthy and Hypertensive Rats
    Article Snippet: The RNA was treated with Ambion DNase I (Invitrogen Life Technologies, Grand Island, NY), which was subsequently removed with Qiagen RNeasy RNA cleanup kit (Qiagen, Germantown, MD). .. SsoFast Evagreen Supermix (BioRad, Hercules, CA) and 500 nM forward and reverse primers were used to carry out quantitative PCR on a CFX96 (BioRad, Hercules, CA) real-time PCR detection system, per manufacturer’s instructions.

    Centrifugation:

    Article Title: Calcium-sensing receptor gene (CASR) polymorphisms and CASR transcript level concerning dyslipidemia in hemodialysis patients: a cross-sectional study
    Article Snippet: They were isolated by density-gradient centrifugation using the Histopaque (Sigma-Aldrich, Missouri, United States). .. RNA samples were treated with Ambion DNase I (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1.5 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using an M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.

    Agarose Gel Electrophoresis:

    Article Title: Calcium-sensing receptor gene (CASR) polymorphisms and CASR transcript level concerning dyslipidemia in hemodialysis patients: a cross-sectional study
    Article Snippet: The concentration and integrity of the isolated RNA were assessed by spectrophotometric quantification and nondenaturing electrophoresis on a 2% agarose gel. .. RNA samples were treated with Ambion DNase I (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1.5 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using an M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.

    Isolation:

    Article Title: Calcium-sensing receptor gene (CASR) polymorphisms and CASR transcript level concerning dyslipidemia in hemodialysis patients: a cross-sectional study
    Article Snippet: The concentration and integrity of the isolated RNA were assessed by spectrophotometric quantification and nondenaturing electrophoresis on a 2% agarose gel. .. RNA samples were treated with Ambion DNase I (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1.5 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using an M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.

    Size-exclusion Chromatography:

    Article Title: Atypical Microglial Response to Biodiesel Exhaust in Healthy and Hypertensive Rats
    Article Snippet: The RNA was treated with Ambion DNase I (Invitrogen Life Technologies, Grand Island, NY), which was subsequently removed with Qiagen RNeasy RNA cleanup kit (Qiagen, Germantown, MD). .. Cycling parameters were 1 cycle at 95o C for 5 min, 40 cycles of 95o C (5 sec.) and 56o C (5 sec.) followed by a melt curve measurement consisting of 5 second 0.5o C incremental increases from 65o C to 95o C. The primer sequences are listed in .

    Electrophoresis:

    Article Title: Calcium-sensing receptor gene (CASR) polymorphisms and CASR transcript level concerning dyslipidemia in hemodialysis patients: a cross-sectional study
    Article Snippet: The concentration and integrity of the isolated RNA were assessed by spectrophotometric quantification and nondenaturing electrophoresis on a 2% agarose gel. .. RNA samples were treated with Ambion DNase I (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1.5 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using an M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.

    Concentration Assay:

    Article Title: Calcium-sensing receptor gene (CASR) polymorphisms and CASR transcript level concerning dyslipidemia in hemodialysis patients: a cross-sectional study
    Article Snippet: The concentration and integrity of the isolated RNA were assessed by spectrophotometric quantification and nondenaturing electrophoresis on a 2% agarose gel. .. RNA samples were treated with Ambion DNase I (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1.5 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using an M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Calcium-sensing receptor gene (CASR) polymorphisms and CASR transcript level concerning dyslipidemia in hemodialysis patients: a cross-sectional study
    Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (qPCR) analysis ... RNA samples were treated with Ambion DNase I (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1.5 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using an M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol.

    Article Title: Atypical Microglial Response to Biodiesel Exhaust in Healthy and Hypertensive Rats
    Article Snippet: Paragraph title: 2.4. Quantitative Reverse Transcriptase Polymerase Chain Reaction ... The RNA was treated with Ambion DNase I (Invitrogen Life Technologies, Grand Island, NY), which was subsequently removed with Qiagen RNeasy RNA cleanup kit (Qiagen, Germantown, MD).

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  • 99
    Thermo Fisher ambion dnase i
    Ambion Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ambion dnase i/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ambion dnase i - by Bioz Stars, 2020-04
    99/100 stars
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