alwi digestion  (New England Biolabs)


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    Structured Review

    New England Biolabs alwi digestion
    Alwi Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alwi digestion/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alwi digestion - by Bioz Stars, 2021-07
    86/100 stars

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    Purification:

    Article Title: Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114
    Article Snippet: After cells were spheroplasted with Zymolyase-20T (MP Biomedicals, Inc.) in spheroplast buffer (1% 2-mercaptoethanol, 1 M sorbitol, 0.1 M EDTA [pH 8.0]), they were lysed and digested in lysis buffer (50 mM EDTA [pH 8.0], 50 mM Tris [pH 8.0], 0.5% SDS, 200 µg of proteinase K). .. At the GAL2 locus, the purified genomic DNA was treated with AccI, BspHI, HhaI and Nt.AlwI (New England BioLabs) for several hours. .. Nt.AlwI was used as a positive control for SSB detection.

    Article Title: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
    Article Snippet: Cells were harvested after 72 h and the genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen). .. After DpnI digestion, adaptor ligation, DpnII digestion, PCR amplification and purification, the amplified DNA was sonicated and digested with AlwI (NEB) to remove the adaptors. .. The DNA libraries were constructed using a NEBNext® Ultra™ DNA Library Prep Kit and were then pooled and subjected to 150 bp paired-ends sequencing on Illumina NovaSeq sequencers.

    Amplification:

    Article Title: FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type specific profiling of protein-DNA interactions in Drosophila melanogaster
    Article Snippet: Following amplification, 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). .. DamID adaptors were removed by AlwI digestion, and 500 ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo-(NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).

    Article Title: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
    Article Snippet: Cells were harvested after 72 h and the genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen). .. After DpnI digestion, adaptor ligation, DpnII digestion, PCR amplification and purification, the amplified DNA was sonicated and digested with AlwI (NEB) to remove the adaptors. .. The DNA libraries were constructed using a NEBNext® Ultra™ DNA Library Prep Kit and were then pooled and subjected to 150 bp paired-ends sequencing on Illumina NovaSeq sequencers.

    Polymerase Chain Reaction:

    Article Title: FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type specific profiling of protein-DNA interactions in Drosophila melanogaster
    Article Snippet: Following amplification, 2 µg DNA was sonicated in a Bioruptor Plus (Diagenode). .. DamID adaptors were removed by AlwI digestion, and 500 ng of the resulting fragments end-repaired with a mix of enzymes (T4 DNA ligase (NEB) + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)), A-tailed with Klenow 3’ to 5’ exo-(NEB), ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). .. The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).

    Article Title: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
    Article Snippet: Cells were harvested after 72 h and the genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen). .. After DpnI digestion, adaptor ligation, DpnII digestion, PCR amplification and purification, the amplified DNA was sonicated and digested with AlwI (NEB) to remove the adaptors. .. The DNA libraries were constructed using a NEBNext® Ultra™ DNA Library Prep Kit and were then pooled and subjected to 150 bp paired-ends sequencing on Illumina NovaSeq sequencers.

    Isolation:

    Article Title: Coordinated repressive chromatin-remodeling of Oct4 and Nanog genes in RA-induced differentiation of embryonic stem cells involves RIP140
    Article Snippet: .. Isolated nuclei from ESCs were digested with 100 U of SapI, AlwI, FokI, XbaI, PstI, EcoRI or StuI (New England Biolabs, Ipswich, MA, USA, www.neb.com ) for 30 min. .. The purified genomic DNA was re-digested with 100 U of AvaI (for Nanog gene) or HindIII (for Oct4 gene).

    Ligation:

    Article Title: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
    Article Snippet: Cells were harvested after 72 h and the genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen). .. After DpnI digestion, adaptor ligation, DpnII digestion, PCR amplification and purification, the amplified DNA was sonicated and digested with AlwI (NEB) to remove the adaptors. .. The DNA libraries were constructed using a NEBNext® Ultra™ DNA Library Prep Kit and were then pooled and subjected to 150 bp paired-ends sequencing on Illumina NovaSeq sequencers.

    Sonication:

    Article Title: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
    Article Snippet: Cells were harvested after 72 h and the genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen). .. After DpnI digestion, adaptor ligation, DpnII digestion, PCR amplification and purification, the amplified DNA was sonicated and digested with AlwI (NEB) to remove the adaptors. .. The DNA libraries were constructed using a NEBNext® Ultra™ DNA Library Prep Kit and were then pooled and subjected to 150 bp paired-ends sequencing on Illumina NovaSeq sequencers.

    Construct:

    Article Title: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
    Article Snippet: After DpnI digestion, adaptor ligation, DpnII digestion, PCR amplification and purification, the amplified DNA was sonicated and digested with AlwI (NEB) to remove the adaptors. .. The DNA libraries were constructed using a NEBNext® Ultra™ DNA Library Prep Kit and were then pooled and subjected to 150 bp paired-ends sequencing on Illumina NovaSeq sequencers. .. DamID-seq data processing For the processing of DamID-seq data, raw reads were trimmed by the Trim Galore software (version 0.4.5) ( https://github.com/FelixKrueger/TrimGalore ).

    Sequencing:

    Article Title: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
    Article Snippet: After DpnI digestion, adaptor ligation, DpnII digestion, PCR amplification and purification, the amplified DNA was sonicated and digested with AlwI (NEB) to remove the adaptors. .. The DNA libraries were constructed using a NEBNext® Ultra™ DNA Library Prep Kit and were then pooled and subjected to 150 bp paired-ends sequencing on Illumina NovaSeq sequencers. .. DamID-seq data processing For the processing of DamID-seq data, raw reads were trimmed by the Trim Galore software (version 0.4.5) ( https://github.com/FelixKrueger/TrimGalore ).

    other:

    Article Title: Synthesizing topological structures containing RNA
    Article Snippet: Digestion with various nucleases Various nucleases were used in this work, including Nt.AlwI (NEB), Nt.BspQI (NEB), RNase H (NEB), DNase I (NEB) and RNase R (Epicentre).

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  • 86
    New England Biolabs alwi digestion
    Alwi Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alwi digestion/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alwi digestion - by Bioz Stars, 2021-07
    86/100 stars
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