allophycocyanin conjugated anti mouse cd151 flow cytometry ab  (R&D Systems)

 
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    Name:
    Mouse CD151 Antibody
    Description:
    The Mouse CD151 Antibody from R D Systems is a rat monoclonal antibody to CD151 This antibody reacts with mouse The Mouse CD151 Antibody has been validated for the following applications Flow Cytometry Immunocytochemistry CyTOF ready
    Catalog Number:
    mab4609
    Price:
    269
    Applications:
    Flow Cytometry, Immunocytochemistry, CyTOF-ready
    Host:
    Rat
    Purity:
    Protein A or G purified from hybridoma culture supernatant
    Conjugate:
    Unconjugated
    Immunogen:
    HEK293 human embryonic kidney cell line transfected with mouse CD151, Met1-Tyr253, Accession # BAA22447
    Size:
    100 ug
    Category:
    Primary Antibodies
    Isotype:
    IgG2a
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    Structured Review

    R&D Systems allophycocyanin conjugated anti mouse cd151 flow cytometry ab
    Mouse CD151 Antibody
    The Mouse CD151 Antibody from R D Systems is a rat monoclonal antibody to CD151 This antibody reacts with mouse The Mouse CD151 Antibody has been validated for the following applications Flow Cytometry Immunocytochemistry CyTOF ready
    https://www.bioz.com/result/allophycocyanin conjugated anti mouse cd151 flow cytometry ab/product/R&D Systems
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    allophycocyanin conjugated anti mouse cd151 flow cytometry ab - by Bioz Stars, 2020-09
    88/100 stars

    Images

    1) Product Images from "Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation"

    Article Title: Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1302874

    CD151 deficiency does not alter basal phenotype of cultured BMMCs. ( A ) CD151 deficiency does not affect growth dynamics of mast cell culture. WT and CD151 −/− BMMCs were cultured in IL-3–conditioned media for 8 wk and the total numbers of live cells in culture were counted at each time point indicated ( left ). Flow cytometry for CD151 expression on peritoneal mast cells (flow cytometry chart) ( middle ), qPCR detection of CD151 mRNA in WT and CD151 −/− BMMCs (bar graph, right ), and Western blot analysis of total-protein lysates for CD151 expression (Western blot, right ) all confirm constitutive CD151 expression in WT mast cells. WT peritoneal mast cells are shown as gray-filled histogram with dotted line and negative control as transparent histogram with dotted line. In immunoblotting, actin was used as a loading control. ( B ) Five-week-old BMMCs from WT and CD151 −/− mice were stained with toluidine blue and images were obtained with an original magnification of ×100. Flow cytometry analysis of FcεRI and c-Kit surface expression and purity of WT and CD151 −/− BMMC cultures. All data are representative of three independent experiments. Data are represented as mean ± SEM. * p
    Figure Legend Snippet: CD151 deficiency does not alter basal phenotype of cultured BMMCs. ( A ) CD151 deficiency does not affect growth dynamics of mast cell culture. WT and CD151 −/− BMMCs were cultured in IL-3–conditioned media for 8 wk and the total numbers of live cells in culture were counted at each time point indicated ( left ). Flow cytometry for CD151 expression on peritoneal mast cells (flow cytometry chart) ( middle ), qPCR detection of CD151 mRNA in WT and CD151 −/− BMMCs (bar graph, right ), and Western blot analysis of total-protein lysates for CD151 expression (Western blot, right ) all confirm constitutive CD151 expression in WT mast cells. WT peritoneal mast cells are shown as gray-filled histogram with dotted line and negative control as transparent histogram with dotted line. In immunoblotting, actin was used as a loading control. ( B ) Five-week-old BMMCs from WT and CD151 −/− mice were stained with toluidine blue and images were obtained with an original magnification of ×100. Flow cytometry analysis of FcεRI and c-Kit surface expression and purity of WT and CD151 −/− BMMC cultures. All data are representative of three independent experiments. Data are represented as mean ± SEM. * p

    Techniques Used: Cell Culture, Flow Cytometry, Cytometry, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Negative Control, Mouse Assay, Staining

    Related Articles

    Flow Cytometry:

    Article Title: Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation
    Article Snippet: .. Allophycocyanin-conjugated anti-mouse CD151 flow cytometry Ab was from R & D Systems (Minneapolis, MN). .. ECL Prime Western blotting detection reagent was from GE Healthcare Biosciences (Pittsburgh, PA).

    other:

    Article Title: Blood pressure influences end-stage renal disease of Cd151 knockout mice
    Article Snippet: Rat mAbs used were MAB1556 against mouse podocalyxin (clone 192703), MAB4609 against mouse Cd151 (R & D Systems Inc.), GoH3 against α6 , MB1.2 against β1 , 346-11A against β4 (BD), and 390 against Cd31 (gift from C. Buck, Wistar Institute, Philadelphia, Pennsylvania, USA; ref. ).

    Cytometry:

    Article Title: Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation
    Article Snippet: .. Allophycocyanin-conjugated anti-mouse CD151 flow cytometry Ab was from R & D Systems (Minneapolis, MN). .. ECL Prime Western blotting detection reagent was from GE Healthcare Biosciences (Pittsburgh, PA).

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    R&D Systems allophycocyanin conjugated anti mouse cd151 flow cytometry ab
    <t>CD151</t> deficiency does not alter basal phenotype of cultured BMMCs. ( A ) CD151 deficiency does not affect growth dynamics of mast cell culture. WT and CD151 −/− BMMCs were cultured in IL-3–conditioned media for 8 wk and the total numbers of live cells in culture were counted at each time point indicated ( left ). Flow <t>cytometry</t> for CD151 expression on peritoneal mast cells (flow cytometry chart) ( middle ), qPCR detection of CD151 mRNA in WT and CD151 −/− BMMCs (bar graph, right ), and Western blot analysis of total-protein lysates for CD151 expression (Western blot, right ) all confirm constitutive CD151 expression in WT mast cells. WT peritoneal mast cells are shown as gray-filled histogram with dotted line and negative control as transparent histogram with dotted line. In immunoblotting, actin was used as a loading control. ( B ) Five-week-old BMMCs from WT and CD151 −/− mice were stained with toluidine blue and images were obtained with an original magnification of ×100. Flow cytometry analysis of FcεRI and c-Kit surface expression and purity of WT and CD151 −/− BMMC cultures. All data are representative of three independent experiments. Data are represented as mean ± SEM. * p
    Allophycocyanin Conjugated Anti Mouse Cd151 Flow Cytometry Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allophycocyanin conjugated anti mouse cd151 flow cytometry ab/product/R&D Systems
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    allophycocyanin conjugated anti mouse cd151 flow cytometry ab - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    CD151 deficiency does not alter basal phenotype of cultured BMMCs. ( A ) CD151 deficiency does not affect growth dynamics of mast cell culture. WT and CD151 −/− BMMCs were cultured in IL-3–conditioned media for 8 wk and the total numbers of live cells in culture were counted at each time point indicated ( left ). Flow cytometry for CD151 expression on peritoneal mast cells (flow cytometry chart) ( middle ), qPCR detection of CD151 mRNA in WT and CD151 −/− BMMCs (bar graph, right ), and Western blot analysis of total-protein lysates for CD151 expression (Western blot, right ) all confirm constitutive CD151 expression in WT mast cells. WT peritoneal mast cells are shown as gray-filled histogram with dotted line and negative control as transparent histogram with dotted line. In immunoblotting, actin was used as a loading control. ( B ) Five-week-old BMMCs from WT and CD151 −/− mice were stained with toluidine blue and images were obtained with an original magnification of ×100. Flow cytometry analysis of FcεRI and c-Kit surface expression and purity of WT and CD151 −/− BMMC cultures. All data are representative of three independent experiments. Data are represented as mean ± SEM. * p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation

    doi: 10.4049/jimmunol.1302874

    Figure Lengend Snippet: CD151 deficiency does not alter basal phenotype of cultured BMMCs. ( A ) CD151 deficiency does not affect growth dynamics of mast cell culture. WT and CD151 −/− BMMCs were cultured in IL-3–conditioned media for 8 wk and the total numbers of live cells in culture were counted at each time point indicated ( left ). Flow cytometry for CD151 expression on peritoneal mast cells (flow cytometry chart) ( middle ), qPCR detection of CD151 mRNA in WT and CD151 −/− BMMCs (bar graph, right ), and Western blot analysis of total-protein lysates for CD151 expression (Western blot, right ) all confirm constitutive CD151 expression in WT mast cells. WT peritoneal mast cells are shown as gray-filled histogram with dotted line and negative control as transparent histogram with dotted line. In immunoblotting, actin was used as a loading control. ( B ) Five-week-old BMMCs from WT and CD151 −/− mice were stained with toluidine blue and images were obtained with an original magnification of ×100. Flow cytometry analysis of FcεRI and c-Kit surface expression and purity of WT and CD151 −/− BMMC cultures. All data are representative of three independent experiments. Data are represented as mean ± SEM. * p

    Article Snippet: Allophycocyanin-conjugated anti-mouse CD151 flow cytometry Ab was from R & D Systems (Minneapolis, MN).

    Techniques: Cell Culture, Flow Cytometry, Cytometry, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Negative Control, Mouse Assay, Staining