Journal: Current Biology
Article Title: A light-sensing system in the common ancestor of the fungi
doi: 10.1016/j.cub.2022.05.034
Figure Lengend Snippet: Confocal microscopic images of fluorescently stained fungal zoospores Rows are organized according to the fungal species to which the zoospores correspond: (A–H) Blastocladiella emersonii ATCC 22665 ( Be ), (I–L) Allomyces macrogynus Australia_3 ( Am ), (M–P) Chytriomyces confervae CBS 675.73 ( Cc ), and (Q–T) Rhizoclosmatium globosum JEL800 ( Rg ). Columns are ordered according to the stains used: (A–Q) Nile red staining for lipid droplets, (B–R) α-tubulin DM1A + Alexa Fluor 647 fluorescence staining for microtubules, (C–S) Alexa Fluor 488 Phalloidin for actin, and (D–T) composite image of all fluorescence channels. Scale bars, 10 μm (A–L) and 5 μm (M–T). Two rows are shown for Be ; the first row is shown to identify the details of the cell body, and the second row shows the extent of the flagellum and the presence of Phalloidin stain (actin) within the flagellum. Supplemental information in FigShare shows a diversity of replicate zoospores imaged using this same approach.
Article Snippet: To stain the lipid components of zoospores, we used Nile red, and to stain the main components of the cytoskeleton, we used α-tubulin DM1A + Alexa Fluor 647 to stain tubulin and Alexa Fluor 488 Phalloidin to stain actin , both of which have previously been used to effectively study zoospore cytoskeletal systems in B. dendrobatidis and R. globosum ., Confocal microscopic images of fluorescently stained fungal zoospores Rows are organized according to the fungal species to which the zoospores correspond: (A–H) Blastocladiella emersonii ATCC 22665 ( Be ), (I–L) Allomyces macrogynus Australia_3 ( Am ), (M–P) Chytriomyces confervae CBS 675.73 ( Cc ), and (Q–T) Rhizoclosmatium globosum JEL800 ( Rg ).
Techniques: Staining, Fluorescence
