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R&D Systems alkaline phosphatase conjugated streptavidin
Alkaline Phosphatase Conjugated Streptavidin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alkaline phosphatase conjugated streptavidin/product/R&D Systems
Average 97 stars, based on 10 article reviews
Price from $9.99 to $1999.99
alkaline phosphatase conjugated streptavidin - by Bioz Stars, 2020-09
97/100 stars

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Recombinant:

Article Title: Cardiac myocyte–secreted cAMP exerts paracrine action via adenosine receptor activation
Article Snippet: .. Human recombinant solubleENTPDase3 and human recombinant soluble alkaline phosphatase (TNAP), expressed in NS0cells from murine myeloma, were obtained from R & D Systems GmbH. .. Human recombinantsoluble ENTPDase1 and -2, and human recombinant soluble NT5E, expressed in CHO cells, werealso obtained from R & D Systems GmbH.

Article Title: Innate Biomineralization
Article Snippet: .. Recombinant human ALPL was purchased from R & D Systems (Minneapolis, MN, USA). .. Rabbit anti-human ALPL antibody, calf intestinal ALP (CIP, ≥10 DEA U/mg), disodium β-glycerophosphate (βGP), phospho(enol)pyruvate monosodium (PEP), pamidronate, dexamethasone, and Alizarin Red S solution were purchased from Sigma-Aldrich (St. Louis, MO, USA).

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    R&D Systems streptavidin conjugated alkaline phosphatase
    Functional inhibition of MIF-1 and MIF-2 by MIF098 and 4-CPPC. A , MIF098 selectively inhibits MIF-1 tautomerase activity compared with MIF-2 measured by HPP tautomerization. B and C , MIF-2–CD74 ( B ) and MIF-1–CD74 ( C ) binding assay using biotinylated human MIF-2 or biotinylated human MIF-1 and immobilized recombinant sCD74. Anti-MIF-1 antibody, anti-MIF-2 antibody, human MIF-1 ( hMIF-1 ), human MIF-2 ( hMIF-2 ), 4-CPPC, and MIF098 were added at increasing concentrations, and biotinylated MIF-2 binding/MIF-1 binding to sCD74 was revealed by detection of <t>streptavidin-conjugated</t> alkaline phosphatase. Nonreactive IgG served as a negative control. D and E , selective inhibition by 4-CPPC or MIF098 of MIF-2 ( D ) and MIF-1 ( E ) stimulated phosphorylation of ERK1/2 in cultured human fibroblasts.
    Streptavidin Conjugated Alkaline Phosphatase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin conjugated alkaline phosphatase/product/R&D Systems
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    streptavidin conjugated alkaline phosphatase - by Bioz Stars, 2020-09
    92/100 stars
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    Functional inhibition of MIF-1 and MIF-2 by MIF098 and 4-CPPC. A , MIF098 selectively inhibits MIF-1 tautomerase activity compared with MIF-2 measured by HPP tautomerization. B and C , MIF-2–CD74 ( B ) and MIF-1–CD74 ( C ) binding assay using biotinylated human MIF-2 or biotinylated human MIF-1 and immobilized recombinant sCD74. Anti-MIF-1 antibody, anti-MIF-2 antibody, human MIF-1 ( hMIF-1 ), human MIF-2 ( hMIF-2 ), 4-CPPC, and MIF098 were added at increasing concentrations, and biotinylated MIF-2 binding/MIF-1 binding to sCD74 was revealed by detection of streptavidin-conjugated alkaline phosphatase. Nonreactive IgG served as a negative control. D and E , selective inhibition by 4-CPPC or MIF098 of MIF-2 ( D ) and MIF-1 ( E ) stimulated phosphorylation of ERK1/2 in cultured human fibroblasts.

    Journal: The Journal of Biological Chemistry

    Article Title: A selective small-molecule inhibitor of macrophage migration inhibitory factor-2 (MIF-2), a MIF cytokine superfamily member, inhibits MIF-2 biological activity

    doi: 10.1074/jbc.RA119.009860

    Figure Lengend Snippet: Functional inhibition of MIF-1 and MIF-2 by MIF098 and 4-CPPC. A , MIF098 selectively inhibits MIF-1 tautomerase activity compared with MIF-2 measured by HPP tautomerization. B and C , MIF-2–CD74 ( B ) and MIF-1–CD74 ( C ) binding assay using biotinylated human MIF-2 or biotinylated human MIF-1 and immobilized recombinant sCD74. Anti-MIF-1 antibody, anti-MIF-2 antibody, human MIF-1 ( hMIF-1 ), human MIF-2 ( hMIF-2 ), 4-CPPC, and MIF098 were added at increasing concentrations, and biotinylated MIF-2 binding/MIF-1 binding to sCD74 was revealed by detection of streptavidin-conjugated alkaline phosphatase. Nonreactive IgG served as a negative control. D and E , selective inhibition by 4-CPPC or MIF098 of MIF-2 ( D ) and MIF-1 ( E ) stimulated phosphorylation of ERK1/2 in cultured human fibroblasts.

    Article Snippet: The wells were washed four times, and streptavidin-conjugated alkaline phosphatase (R & D Systems) was added to each well for 1 h of incubation at room temperature in the dark.

    Techniques: Functional Assay, Inhibition, Activity Assay, Binding Assay, Recombinant, Negative Control, Cell Culture