alkaline phosphatase conjugated streptavidin  (Promega)

 
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    Name:
    Streptavidin
    Description:
    Has very high affinity Kd 10 15M for the vitamin biotin Each subunit binds one biotin molecule
    Catalog Number:
    z7041
    Price:
    None
    Category:
    Instrumentation Labware Biochemicals Labware Biochemical Buffers and Reagents
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    Structured Review

    Promega alkaline phosphatase conjugated streptavidin
    Has very high affinity Kd 10 15M for the vitamin biotin Each subunit binds one biotin molecule
    https://www.bioz.com/result/alkaline phosphatase conjugated streptavidin/product/Promega
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase conjugated streptavidin - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2
    Article Snippet: .. We obtained Edinburgh minimal medium (EMM) and Complete Supplement Mixture (CSM) from MP Biomedicals; yeast extract, peptone, agar from Becton Dickson; oligonucleotides from Integrated DNA Technologies; complete EDTA-free protease inhibitor from Roche; amino acids, D-sorbitol, D-Galactose, yeast nitrogen base without amino acids, sodium ascorbate, (S)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) from Sigma-Aldrich; Prestained Protein Standards from Bio-Rad; Biotin-phenol from Iris Biotech; Streptavidin magnetic beads from Promega; Gibson Assembly Master Mix from NEB BioLabs; Zymolyase from Seikagaku distributed by MP Biomedicals. .. Wild-type haploid S. pombe ( h- leu1-32 ura4-D18 ade6-M210 his3-D1 ) and derived strains were grown to exponential phase at 30°C in EMM medium (32.2 g/L EMM plus 20 g/L glucose supplemented with 225 mg/L each of adenine, uracil, leucine, histidine, and lysine) ( ).

    Incubation:

    Article Title: The Escherichia coli Cryptic Prophage Protein YfdR Binds to DnaA and Initiation of Chromosomal Replication Is Inhibited by Overexpression of the Gene Cluster yfdQ-yfdR-yfdS-yfdT
    Article Snippet: .. The mixtures were further incubated for 1 h at 4°C, with gentle rotation, in the presence of streptavidin-conjugated magnetic beads (Promega). ..

    other:

    Article Title: Direct Evidence for Spontaneous Branch Migration in Antiparallel DNA Holliday Junctions
    Article Snippet: The extra strand is added to the restriction digest, and after restriction the molecules are allowed to equilibrate for two days at 4 °C ( ) or at room temperature (not shown but identical to ), before treatment with streptavidin beads. illustrates both the efficacy of restriction and the absence of dissociation.

    Article Title: Direct Evidence for Spontaneous Branch Migration in Antiparallel DNA Holliday Junctions
    Article Snippet: Streptavidin beads (50 μL) (Promega), stored at 4 °C in a solution containing phosphate-buffered saline (PBS), 1 mg/ml BSA, and 0.02% sodium azide, are put into a siliconized tube on a magnetic stand and allowed to settle for 5–10 min.

    Article Title: Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement
    Article Snippet: The TNT transcription–translation system, Transcend Non-Radioactive Translation Detection Systems, trypsin, and peroxidase-labeled streptavidin were from Promega.

    Purification:

    Article Title: Direct Evidence for Spontaneous Branch Migration in Antiparallel DNA Holliday Junctions
    Article Snippet: .. The system can be purified of these contaminants by treatment with streptavidin beads, as performed previously ( ). ..

    Magnetic Beads:

    Article Title: The Escherichia coli Cryptic Prophage Protein YfdR Binds to DnaA and Initiation of Chromosomal Replication Is Inhibited by Overexpression of the Gene Cluster yfdQ-yfdR-yfdS-yfdT
    Article Snippet: .. The mixtures were further incubated for 1 h at 4°C, with gentle rotation, in the presence of streptavidin-conjugated magnetic beads (Promega). ..

    Article Title: Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2
    Article Snippet: .. We obtained Edinburgh minimal medium (EMM) and Complete Supplement Mixture (CSM) from MP Biomedicals; yeast extract, peptone, agar from Becton Dickson; oligonucleotides from Integrated DNA Technologies; complete EDTA-free protease inhibitor from Roche; amino acids, D-sorbitol, D-Galactose, yeast nitrogen base without amino acids, sodium ascorbate, (S)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) from Sigma-Aldrich; Prestained Protein Standards from Bio-Rad; Biotin-phenol from Iris Biotech; Streptavidin magnetic beads from Promega; Gibson Assembly Master Mix from NEB BioLabs; Zymolyase from Seikagaku distributed by MP Biomedicals. .. Wild-type haploid S. pombe ( h- leu1-32 ura4-D18 ade6-M210 his3-D1 ) and derived strains were grown to exponential phase at 30°C in EMM medium (32.2 g/L EMM plus 20 g/L glucose supplemented with 225 mg/L each of adenine, uracil, leucine, histidine, and lysine) ( ).

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  • 99
    Promega alkaline phosphatase conjugated streptavidin
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline phosphatase conjugated streptavidin/product/Promega
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase conjugated streptavidin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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