alkaline phosphatase conjugated streptavidin  (Pharmingen)

 
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    Pharmingen alkaline phosphatase conjugated streptavidin
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline phosphatase conjugated streptavidin/product/Pharmingen
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase conjugated streptavidin - by Bioz Stars, 2020-09
    92/100 stars

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    Incubation:

    Article Title: Natural IgE promotes epithelial hyperplasia and inflammation-driven tumour growth
    Article Snippet: .. After washing in PBS 0.05% Tween, plates were incubated with alkaline phosphatase-conjugated Streptavidin (PharMingen) for 1 hr at 37°C. .. After washing, detection of antibody levels was carried out by addition of alkaline phosphatase substrate pNPP (Sigma) and absorbance measured at 405 nm.

    Article Title: Superior Immunogenicity of Inactivated Whole Virus H5N1 Influenza Vaccine is Primarily Controlled by Toll-like Receptor Signalling
    Article Snippet: .. Subsequently, the plates were incubated with 100 µl of alkaline phosphatase conjugated streptavidin (Pharmingen) in 2% BSA/PBS for 1 h at 37°C, spots were visualized with 5-bromo-4-chloro-3-indolylphosphate (Sigma-Aldrich) substrate immobilized in solidified agarose. .. Plasmacytoid dendritic cells Plasmacytoid DCs were generated from bone marrow cells of C57BL/6 or TLR7−/− mice by seeding 1–2×106 bone marrow cells per well of a 24-well plate and culturing the cells for one week in Iscove's Modified Dulbecoo's Medium (IMDM) with 10% FCS and 100 ng/ml FLT3L (R & D Systems, Abingdon, UK) .

    Article Title: Epithelial damage and tissue γδ T cells promote a unique tumor-protective IgE response
    Article Snippet: .. After washing in PBS 0.05% Tween, plates were incubated with alkaline phosphatase-conjugated Streptavidin (PharMingen) for 1 hr at 37°C. .. After washing, detection of antibody levels was carried out by addition of alkaline phosphatase substrate pNPP (Sigma) and absorbance measured at 405 nm.

    other:

    Article Title: Effect of Attenuation of Treg during BCG Immunization on Anti-Mycobacterial Th1 Responses and Protection against Mycobacterium tuberculosis
    Article Snippet: Biotinylated anti-IFN-γ mAb (XMG1.2), alkaline phosphatase-conjugated streptavidin (PharMingen, Le Pont de Claix, France) and 5-Bromo-5-Chloro-3-Indolyl Phosphate/Nitro“Blue” Tetrazolium (Sigma), as phosphatase substrate, were used to reveal the spots, automatically counted by use of a Bioreader-3000 Pro (BIO-SYS, Karben, Germany).

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  • 92
    Pharmingen alkaline phosphatase conjugated streptavidin
    Alkaline Phosphatase Conjugated Streptavidin, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline phosphatase conjugated streptavidin/product/Pharmingen
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase conjugated streptavidin - by Bioz Stars, 2020-09
    92/100 stars
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