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Agilent technologies alkaline phosphatase conjugated streptavidin
Characterisation of ppIgG. (A) Pig plasma IgG product (ppIgG, 1 mg/ml) was run under reducing conditions on 12% SDS PAGE as described in Materials and Methods. In parallel, Western blotting (WB) was performed on the same sample. The blot was developed with biotinylated rabbit anti-pig IgG F(ab) 2 antibody, followed by alkaline phosphatase-coupled <t>streptavidin</t> (see Materials and Methods ). (B) Wells were coated with formalin-fixed bacteria (Y. ruckeri, S. diarizonae, E. coli O138, O149:F4 and F18+) in separate ELISA plates, and incubated with ppIgG added in serial 2-fold dilutions, and the bound IgG was estimated by using a HRP-conjugated rabbit anti-pig IgG antibody. Data from one typical experiment are presented. (C) ppIgG was tested for reactivity against Escherichia coli and Salmonella diarizonae by competitive ELISA as described ( Materials and Methods ). ppIgG heated at 60°C or 70°C for 1 hour and PBS were used as control samples. The results are presented as ‘% inhibition’ of the signal obtained in the absence of ppIgG (median values (± median ranges)). Two-way ANOVA (subjected to Bonferonni post-test) was used to observe statistical significance between the treated and untreated ppIgG (***: p
Alkaline Phosphatase Conjugated Streptavidin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Natural Pig Plasma Immunoglobulins Have Anti-Bacterial Effects: Potential for Use as Feed Supplement for Treatment of Intestinal Infections in Pigs"

Article Title: Natural Pig Plasma Immunoglobulins Have Anti-Bacterial Effects: Potential for Use as Feed Supplement for Treatment of Intestinal Infections in Pigs

Journal: PLoS ONE

doi: 10.1371/journal.pone.0147373

Characterisation of ppIgG. (A) Pig plasma IgG product (ppIgG, 1 mg/ml) was run under reducing conditions on 12% SDS PAGE as described in Materials and Methods. In parallel, Western blotting (WB) was performed on the same sample. The blot was developed with biotinylated rabbit anti-pig IgG F(ab) 2 antibody, followed by alkaline phosphatase-coupled streptavidin (see Materials and Methods ). (B) Wells were coated with formalin-fixed bacteria (Y. ruckeri, S. diarizonae, E. coli O138, O149:F4 and F18+) in separate ELISA plates, and incubated with ppIgG added in serial 2-fold dilutions, and the bound IgG was estimated by using a HRP-conjugated rabbit anti-pig IgG antibody. Data from one typical experiment are presented. (C) ppIgG was tested for reactivity against Escherichia coli and Salmonella diarizonae by competitive ELISA as described ( Materials and Methods ). ppIgG heated at 60°C or 70°C for 1 hour and PBS were used as control samples. The results are presented as ‘% inhibition’ of the signal obtained in the absence of ppIgG (median values (± median ranges)). Two-way ANOVA (subjected to Bonferonni post-test) was used to observe statistical significance between the treated and untreated ppIgG (***: p
Figure Legend Snippet: Characterisation of ppIgG. (A) Pig plasma IgG product (ppIgG, 1 mg/ml) was run under reducing conditions on 12% SDS PAGE as described in Materials and Methods. In parallel, Western blotting (WB) was performed on the same sample. The blot was developed with biotinylated rabbit anti-pig IgG F(ab) 2 antibody, followed by alkaline phosphatase-coupled streptavidin (see Materials and Methods ). (B) Wells were coated with formalin-fixed bacteria (Y. ruckeri, S. diarizonae, E. coli O138, O149:F4 and F18+) in separate ELISA plates, and incubated with ppIgG added in serial 2-fold dilutions, and the bound IgG was estimated by using a HRP-conjugated rabbit anti-pig IgG antibody. Data from one typical experiment are presented. (C) ppIgG was tested for reactivity against Escherichia coli and Salmonella diarizonae by competitive ELISA as described ( Materials and Methods ). ppIgG heated at 60°C or 70°C for 1 hour and PBS were used as control samples. The results are presented as ‘% inhibition’ of the signal obtained in the absence of ppIgG (median values (± median ranges)). Two-way ANOVA (subjected to Bonferonni post-test) was used to observe statistical significance between the treated and untreated ppIgG (***: p

Techniques Used: SDS Page, Western Blot, Enzyme-linked Immunosorbent Assay, Incubation, Competitive ELISA, Inhibition

2) Product Images from "Deficiency in CD22, a B Cell-specific Inhibitory Receptor, Is Sufficient to Predispose to Development of High Affinity Autoantibodies "

Article Title: Deficiency in CD22, a B Cell-specific Inhibitory Receptor, Is Sufficient to Predispose to Development of High Affinity Autoantibodies

Journal: The Journal of Experimental Medicine

doi:

Binding kinetics of the monoclonal anti-DNA antibodies as measured by surface plasmon resonance. Antibody binding to a biotinylated ds oligodeoxyribonucleotide immobilized on streptavidin bound to the chip is depicted in resonance units and was monitored as a function of time. The dissociation half-life (min) calculated for each antibody is indicated in parentheses. The S19 (anticardiolipin) antibody (center) was included as a negative control.
Figure Legend Snippet: Binding kinetics of the monoclonal anti-DNA antibodies as measured by surface plasmon resonance. Antibody binding to a biotinylated ds oligodeoxyribonucleotide immobilized on streptavidin bound to the chip is depicted in resonance units and was monitored as a function of time. The dissociation half-life (min) calculated for each antibody is indicated in parentheses. The S19 (anticardiolipin) antibody (center) was included as a negative control.

Techniques Used: Binding Assay, SPR Assay, Chromatin Immunoprecipitation, Negative Control

3) Product Images from "Antibodies to human fetal erythroid cells from a nonimmune phage antibody library"

Article Title: Antibodies to human fetal erythroid cells from a nonimmune phage antibody library

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.051631798

Staining of total fetal liver by a fetal NRBC specific phage antibody. Fetal liver cells were incubated with biotinylated phage antibody FSG9, applied to microscope slides, and then stained with FITC anti-fetal hemoglobin antibody ( A ), streptavidin Alexa fluor 546 to detect phage binding ( B ), or 4′,6-diamidino-2-phenylindole ( D ). Fetal hemoglobin and phage binding were visualized together with a dual band filter ( C ). Only fetal NRBCs stained with both. r, enucleated RBC; w, WBC.
Figure Legend Snippet: Staining of total fetal liver by a fetal NRBC specific phage antibody. Fetal liver cells were incubated with biotinylated phage antibody FSG9, applied to microscope slides, and then stained with FITC anti-fetal hemoglobin antibody ( A ), streptavidin Alexa fluor 546 to detect phage binding ( B ), or 4′,6-diamidino-2-phenylindole ( D ). Fetal hemoglobin and phage binding were visualized together with a dual band filter ( C ). Only fetal NRBCs stained with both. r, enucleated RBC; w, WBC.

Techniques Used: Staining, Incubation, Microscopy, Binding Assay

Staining of adult buffy coat WBCs and total fetal liver by phage antibodies. Adult buffy coat WBCs or fetal liver cells were stained with biotinylated phage antibodies and the cells applied to microscope slides, fixed, and stained with streptavidin-alkaline phosphatase and Fast Red. The phage antibody FSA7 stains fetal erythroid cells ( A ) but not WBCs ( C ), whereas the phage antibody FSH3 stains both fetal liver ( B ) and a subset of WBCs ( D ).
Figure Legend Snippet: Staining of adult buffy coat WBCs and total fetal liver by phage antibodies. Adult buffy coat WBCs or fetal liver cells were stained with biotinylated phage antibodies and the cells applied to microscope slides, fixed, and stained with streptavidin-alkaline phosphatase and Fast Red. The phage antibody FSA7 stains fetal erythroid cells ( A ) but not WBCs ( C ), whereas the phage antibody FSH3 stains both fetal liver ( B ) and a subset of WBCs ( D ).

Techniques Used: Staining, Microscopy

Related Articles

High Performance Liquid Chromatography:

Article Title: Circular Permutation Directs Orthogonal Assembly in Complex Collagen Peptide Mixtures *
Article Snippet: .. For the mixtures before the streptavidin-biotin binding assay, each peptide of 250 n m , e.g. 3.75 μl of the mixtures with 0.4 m m total concentration, were prepared in 0.2% trifluoroacetic acid (TFA) and loaded on to a pre-packed Poroshell 120, EC-C18, 2.1 × 100 mm, 2.7-μm column (Agilent Technology) in 0.2% TFA/water as the mobile phase A and eluted with a linear gradient of 2–25% of 0.2% TFA in acetonitrile (phase B) in 40 min. HPLC profiles were monitored as UV absorbance at 214 nm. .. Due to identical molecular weights, original and permuted sequences had similar HPLC retention.

Immunohistochemistry:

Article Title: Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study
Article Snippet: .. The immunohistochemical procedure (streptavidin biotin- peroxidase method, (LSAB Kit; Dako, Glostrup, Denmark) was the same as that used by the authors in a previous study [ ]. .. Antibodies were diluted in an antibody diluent (Dako, Glostrup, Denmark) and applied overnight at 4 °C.

Amplification:

Article Title: IFN-? Is Constitutively Expressed in the Human Thymus, but Not in Peripheral Lymphoid Organs
Article Snippet: .. 4 um sections were mounted on slides and stained with a monoclonal antibody to LL-37 (1∶50) or CD19 (1∶50) and control IgG2a antibody followed by biotinylated rabbit anti-mouse antibody and streptavidin-biotin-peroxidase complex using the DAKO Catalyzed Signal Amplification System. .. Isolation and stimulation of thymic pDC pDC were isolated from human thymus tissue by MACS-based negative selection (Miltenyi).

Concentration Assay:

Article Title: Circular Permutation Directs Orthogonal Assembly in Complex Collagen Peptide Mixtures *
Article Snippet: .. For the mixtures before the streptavidin-biotin binding assay, each peptide of 250 n m , e.g. 3.75 μl of the mixtures with 0.4 m m total concentration, were prepared in 0.2% trifluoroacetic acid (TFA) and loaded on to a pre-packed Poroshell 120, EC-C18, 2.1 × 100 mm, 2.7-μm column (Agilent Technology) in 0.2% TFA/water as the mobile phase A and eluted with a linear gradient of 2–25% of 0.2% TFA in acetonitrile (phase B) in 40 min. HPLC profiles were monitored as UV absorbance at 214 nm. .. Due to identical molecular weights, original and permuted sequences had similar HPLC retention.

Incubation:

Article Title: Immunocapture and Identification of Cell Membrane Protein Antigenic Targets of Serum Autoantibodies *
Article Snippet: .. To immunostain bands of biotinylated proteins, filters were incubated in HRP-conjugated streptavidin (Dako catalog number P0397) diluted 1:10,000 in PBS Tween for 45 min at room temperature, washed in PBS Tween, and stained with 3,3-diaminobenzidine tetrahydrochloride (DAB; Sigma) color reagent (0.5 mg/ml DAB in PBS with 0.03% hydrogen peroxide) for between 5 and 15 min. To immunostain specifically for MuSK, the filter was incubated in goat polyclonal anti-MuSK extracellular domain antibody (R & D Systems catalog number AF562) diluted 1:200 in PBS Tween for 45 min at room temperature, washed in PBS Tween, and incubated in HRP-conjugated anti-goat immunoglobulins secondary antibody (Dako catalog number P0449) diluted 1:1000 in PBS Tween for 30 min at room temperature before DAB color development. .. Digestion and Preparation of Immunoprecipitation Products Prior to Liquid Chromatography and Mass Spectrometry Immunoprecipitation products, eluted in glycine-HCl and neutralized as described above, were lyophilized in a vacuum centrifuge.

Article Title: Natural Pig Plasma Immunoglobulins Have Anti-Bacterial Effects: Potential for Use as Feed Supplement for Treatment of Intestinal Infections in Pigs
Article Snippet: .. Then, membranes were incubated with polyclonal biotinylated rabbit anti-Pig F(ab)2 IgG (LSBio, Copenhagen, Denmark) diluted 1:10.000 in TBS-T for 1 hour followed by 3x10 min washes in TBS-T before the last incubation with alkaline phosphatase-conjugated streptavidin (DAKO, Glostrup, Denmark) 1:3000 in TBS-T (1 hour). .. After 3x50 ml washes in TBS-T, blots were developed with 4-nitro-blue-tetrazolium/5-bromo-4-chlor o-3-indolyl-phosphate (NBT/BCIP) tablets (Roche, Hvidovre, Denmark) following the manufacturer’s instructions, terminating colour development by washing the blot with several changes of MilliQ water.

Article Title: Identification of Vimentin as a Potential Therapeutic Target against HIV Infection
Article Snippet: .. Cells were incubated for 1 h at 37 °C with streptavidin-FITC (Dako, Glostrup, Denmark). .. A final incubation step with 10 ug/mL propidium iodine (Sigma-Aldrich, USA) over 30 s was implemented for nuclear staining.

Article Title: Both ?2,3- and ?2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus
Article Snippet: .. Sections were then rinsed 3 times with PBS, and incubated with biotinylated secondary antibody (Dako, USA) and peroxidase-conjugated streptavidin (Dako, USA). .. Reactions were developed with 3-amino-9-ethylcarbazol (AEC; Vector laboratories, USA), and followed by Mayer's hemalum solution (Merck, Germany) for counterstaining.

Staining:

Article Title: Immunocapture and Identification of Cell Membrane Protein Antigenic Targets of Serum Autoantibodies *
Article Snippet: .. To immunostain bands of biotinylated proteins, filters were incubated in HRP-conjugated streptavidin (Dako catalog number P0397) diluted 1:10,000 in PBS Tween for 45 min at room temperature, washed in PBS Tween, and stained with 3,3-diaminobenzidine tetrahydrochloride (DAB; Sigma) color reagent (0.5 mg/ml DAB in PBS with 0.03% hydrogen peroxide) for between 5 and 15 min. To immunostain specifically for MuSK, the filter was incubated in goat polyclonal anti-MuSK extracellular domain antibody (R & D Systems catalog number AF562) diluted 1:200 in PBS Tween for 45 min at room temperature, washed in PBS Tween, and incubated in HRP-conjugated anti-goat immunoglobulins secondary antibody (Dako catalog number P0449) diluted 1:1000 in PBS Tween for 30 min at room temperature before DAB color development. .. Digestion and Preparation of Immunoprecipitation Products Prior to Liquid Chromatography and Mass Spectrometry Immunoprecipitation products, eluted in glycine-HCl and neutralized as described above, were lyophilized in a vacuum centrifuge.

Article Title: IFN-? Is Constitutively Expressed in the Human Thymus, but Not in Peripheral Lymphoid Organs
Article Snippet: .. 4 um sections were mounted on slides and stained with a monoclonal antibody to LL-37 (1∶50) or CD19 (1∶50) and control IgG2a antibody followed by biotinylated rabbit anti-mouse antibody and streptavidin-biotin-peroxidase complex using the DAKO Catalyzed Signal Amplification System. .. Isolation and stimulation of thymic pDC pDC were isolated from human thymus tissue by MACS-based negative selection (Miltenyi).

Binding Assay:

Article Title: Circular Permutation Directs Orthogonal Assembly in Complex Collagen Peptide Mixtures *
Article Snippet: .. For the mixtures before the streptavidin-biotin binding assay, each peptide of 250 n m , e.g. 3.75 μl of the mixtures with 0.4 m m total concentration, were prepared in 0.2% trifluoroacetic acid (TFA) and loaded on to a pre-packed Poroshell 120, EC-C18, 2.1 × 100 mm, 2.7-μm column (Agilent Technology) in 0.2% TFA/water as the mobile phase A and eluted with a linear gradient of 2–25% of 0.2% TFA in acetonitrile (phase B) in 40 min. HPLC profiles were monitored as UV absorbance at 214 nm. .. Due to identical molecular weights, original and permuted sequences had similar HPLC retention.

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    Agilent technologies alkaline phosphatase red streptavidin alkaline phosphatase ap
    Alkaline Phosphatase Red Streptavidin Alkaline Phosphatase Ap, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline phosphatase red streptavidin alkaline phosphatase ap/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase red streptavidin alkaline phosphatase ap - by Bioz Stars, 2020-09
    99/100 stars
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