alexa fluor 647 conjugated goat ig anti mouse igg antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher alexa fluor 647 conjugated goat ig anti mouse igg antibody
    Alexa Fluor 647 Conjugated Goat Ig Anti Mouse Igg Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated goat ig anti mouse igg antibody/product/Thermo Fisher
    Average 81 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 conjugated goat ig anti mouse igg antibody - by Bioz Stars, 2020-01
    81/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: The PCR products were cloned as downstream fusions to Aga2 and Xpress epitope tag genes in the yeast surface display vector pYD1 (Invitrogen), under the control of an upstream GAL1 promoter. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    Immunocytochemistry:

    Article Title: Receptor-stimulated Transamidation Induces Activation of Rac1 and Cdc42 and the Regulation of Dendritic Spines
    Article Snippet: Paragraph title: 2.4 Immunocytochemistry ... Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) antibody and Alexa Fluor® 647 conjugated goat anti-mouse IgG (H+L) secondary antibody (Life Technologies, Grand Island, NY) were used to target primary antibodies.

    Synthesized:

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. A set of 11 overlapping peptides, each comprising 10–12 amino acids from DENV-2 domain III was synthesized and purified by HPLC.

    Cytometry:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: Paragraph title: Flow cytometry ... To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA).

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: Cells were then stained with Hoechst 33342 for 30 min. For analysis of labeled mitosis, the Click-iT EdU Pacific Blue Flow Cytometry Assay kit (Invitrogen) was used in combination with an M-phase marker, phospho-histone H3. .. Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice.

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. After fixation with 1 % paraformaldehyde in PBS, yeast cells were analysed on a FACS Scan flow cytometer (Becton Dickinson) using FlowJo software (Tree Star).

    Article Title: Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
    Article Snippet: .. Flow cytometer analysis Surface expression of EGFR in human hepatocytes, PC3, SKBR3, SK-MES-1, Hut125, Caski, HT29, H2170, LS174T, HepG2, SW480, MDA-MB-231, MDA-MB-468 and BT-20 cells was determined by staining the cells with monoclonal mouse IgG anti-human EGFR (Santa Cruz Biotechnology, 5 μg ml−1 in staining buffer (PBS containing 0.1% bovine serum albumin) for 30 min at 4 °C followed by Alexa Fluor 647-conjugated goat Ig anti-mouse IgG antibody (Thermo Fisher Scientific, 5 μg ml−1 ). .. Unbound antibodies were removed by washing with cold PBS twice and the surface fluorescence of 104 viable cells was measured by FACScaliber flow cytometer (Becton Dickinson) and analysed with Flowjo (Tree Star Inc.).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. Random mutant libraries of E2 were generated from genotype 1a (strain H77) and genotype 2a (strain J6) genes by error-prone PCR using a GeneMorph II random-mutagenesis kit (Stratagene).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes). .. After fixation with 1% paraformaldehyde in PBS, yeast cells were analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software.

    Construct:

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Expression constructs were transformed into Saccharomyces cerevisiae strain EBY100 ( ) using the S . c . .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: These constructs were transformed into Saccharomyces cerevisiae strain EBY100 , using S.c. EasyComp Transformation Kit (Invitrogen) to generate yeast that expressed DENV-1 DI-DII or DIII. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    Incubation:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: Single cell samples were incubated with live/dead green dye ( , Life Technologies, USA) and the indicated antibodies or isotype controls in staining buffer (PBS containing 2% FBS) for 30 mins at 4°C and then fixed in 1% paraformaldehyde. .. To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA).

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: .. Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice. .. After staining, all samples were washed in PBS.

    Article Title: Epigenetic Regulation Alters Biofilm Architecture and Composition in Multiple Clinical Isolates of Nontypeable Haemophilus influenzae
    Article Snippet: .. After 16 h, biofilms were rinsed twice with DPBS to remove nonadherent bacteria and then incubated with anti-NTHI HU polyclonal rabbit sera (1:200) for 30 min or commercially available murine antibody against dsDNA (AbCam) (1:200) for 1 h. Biofilms were then rinsed and incubated for 15 min with goat anti-rabbit or goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Thermo Fisher, Waltham, MA). .. Biofilms were rinsed with DPBS and imaged with a Zeiss 700 Meta-laser scanning confocal microscope.

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: .. After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. After fixation with 1 % paraformaldehyde in PBS, yeast cells were analysed on a FACS Scan flow cytometer (Becton Dickinson) using FlowJo software (Tree Star).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. Random mutant libraries of E2 were generated from genotype 1a (strain H77) and genotype 2a (strain J6) genes by error-prone PCR using a GeneMorph II random-mutagenesis kit (Stratagene).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes). .. After fixation with 1% paraformaldehyde in PBS, yeast cells were analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software.

    Expressing:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: .. To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA). .. For p-Akt determination, intracellular staining was performed with FITC-conjugated anti-p-Akt (s473) (eBioscience, USA).

    Article Title: Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
    Article Snippet: .. Flow cytometer analysis Surface expression of EGFR in human hepatocytes, PC3, SKBR3, SK-MES-1, Hut125, Caski, HT29, H2170, LS174T, HepG2, SW480, MDA-MB-231, MDA-MB-468 and BT-20 cells was determined by staining the cells with monoclonal mouse IgG anti-human EGFR (Santa Cruz Biotechnology, 5 μg ml−1 in staining buffer (PBS containing 0.1% bovine serum albumin) for 30 min at 4 °C followed by Alexa Fluor 647-conjugated goat Ig anti-mouse IgG antibody (Thermo Fisher Scientific, 5 μg ml−1 ). .. Unbound antibodies were removed by washing with cold PBS twice and the surface fluorescence of 104 viable cells was measured by FACScaliber flow cytometer (Becton Dickinson) and analysed with Flowjo (Tree Star Inc.).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Protein expression was induced by cultivating yeast for an additional 48 to 72 h in tryptophan-free medium supplemented with 2% galactose at 20°C. .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: Fusion protein expression was induced on the surface by growing yeast for additional 48 hrs in tryptophan-free media containing 2% galactose at 20°C. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    Transformation Assay:

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: EasyComp transformation Kit (Invitrogen). .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: These constructs were transformed into Saccharomyces cerevisiae strain EBY100 , using S.c. EasyComp Transformation Kit (Invitrogen) to generate yeast that expressed DENV-1 DI-DII or DIII. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    High Performance Liquid Chromatography:

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. A set of 11 overlapping peptides, each comprising 10–12 amino acids from DENV-2 domain III was synthesized and purified by HPLC.

    Flow Cytometry:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: Paragraph title: Flow cytometry ... To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA).

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: Paragraph title: Flow-cytometric analysis ... Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice.

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. After fixation with 1 % paraformaldehyde in PBS, yeast cells were analysed on a FACS Scan flow cytometer (Becton Dickinson) using FlowJo software (Tree Star).

    Article Title: Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
    Article Snippet: .. Flow cytometer analysis Surface expression of EGFR in human hepatocytes, PC3, SKBR3, SK-MES-1, Hut125, Caski, HT29, H2170, LS174T, HepG2, SW480, MDA-MB-231, MDA-MB-468 and BT-20 cells was determined by staining the cells with monoclonal mouse IgG anti-human EGFR (Santa Cruz Biotechnology, 5 μg ml−1 in staining buffer (PBS containing 0.1% bovine serum albumin) for 30 min at 4 °C followed by Alexa Fluor 647-conjugated goat Ig anti-mouse IgG antibody (Thermo Fisher Scientific, 5 μg ml−1 ). .. Unbound antibodies were removed by washing with cold PBS twice and the surface fluorescence of 104 viable cells was measured by FACScaliber flow cytometer (Becton Dickinson) and analysed with Flowjo (Tree Star Inc.).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. Random mutant libraries of E2 were generated from genotype 1a (strain H77) and genotype 2a (strain J6) genes by error-prone PCR using a GeneMorph II random-mutagenesis kit (Stratagene).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes). .. After fixation with 1% paraformaldehyde in PBS, yeast cells were analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software.

    Mutagenesis:

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: Wild-type or mutant DENV-2 domain III displayed on yeast were harvested, washed with PBS supplemented with BSA (1 mg ml−1 ) and stained with 50 μl diluted mAbs (9F12, 3H5-1). .. After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. Random mutant libraries of E2 were generated from genotype 1a (strain H77) and genotype 2a (strain J6) genes by error-prone PCR using a GeneMorph II random-mutagenesis kit (Stratagene).

    Generated:

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: DENV-2 domain III mutants that were generated as part of a random library by error-prone mutagenesis in the pYD1 vector were expressed on the surface of yeast as described previously ( ). .. After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. Random mutant libraries of E2 were generated from genotype 1a (strain H77) and genotype 2a (strain J6) genes by error-prone PCR using a GeneMorph II random-mutagenesis kit (Stratagene).

    Polymerase Chain Reaction:

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. Random mutant libraries of E2 were generated from genotype 1a (strain H77) and genotype 2a (strain J6) genes by error-prone PCR using a GeneMorph II random-mutagenesis kit (Stratagene).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: The PCR products were cloned as downstream fusions to Aga2 and Xpress epitope tag genes in the yeast surface display vector pYD1 (Invitrogen), under the control of an upstream GAL1 promoter. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    Fluorescence:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: Fluorescence data were collected on a FACS machine (FACS Canto II, Becton-Dickinson, USA). .. To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA).

    Article Title: Epigenetic Regulation Alters Biofilm Architecture and Composition in Multiple Clinical Isolates of Nontypeable Haemophilus influenzae
    Article Snippet: After 16 h, biofilms were rinsed twice with DPBS to remove nonadherent bacteria and then incubated with anti-NTHI HU polyclonal rabbit sera (1:200) for 30 min or commercially available murine antibody against dsDNA (AbCam) (1:200) for 1 h. Biofilms were then rinsed and incubated for 15 min with goat anti-rabbit or goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Thermo Fisher, Waltham, MA). .. Relative HU and eDNA abundance was calculated as total fluorescence in the Alexa Fluor 647 channel divided by total biomass in the GFP channel, multiplied by 100.

    Article Title: Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
    Article Snippet: Flow cytometer analysis Surface expression of EGFR in human hepatocytes, PC3, SKBR3, SK-MES-1, Hut125, Caski, HT29, H2170, LS174T, HepG2, SW480, MDA-MB-231, MDA-MB-468 and BT-20 cells was determined by staining the cells with monoclonal mouse IgG anti-human EGFR (Santa Cruz Biotechnology, 5 μg ml−1 in staining buffer (PBS containing 0.1% bovine serum albumin) for 30 min at 4 °C followed by Alexa Fluor 647-conjugated goat Ig anti-mouse IgG antibody (Thermo Fisher Scientific, 5 μg ml−1 ). .. Unbound antibodies were removed by washing with cold PBS twice and the surface fluorescence of 104 viable cells was measured by FACScaliber flow cytometer (Becton Dickinson) and analysed with Flowjo (Tree Star Inc.).

    Multiple Displacement Amplification:

    Article Title: Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
    Article Snippet: .. Flow cytometer analysis Surface expression of EGFR in human hepatocytes, PC3, SKBR3, SK-MES-1, Hut125, Caski, HT29, H2170, LS174T, HepG2, SW480, MDA-MB-231, MDA-MB-468 and BT-20 cells was determined by staining the cells with monoclonal mouse IgG anti-human EGFR (Santa Cruz Biotechnology, 5 μg ml−1 in staining buffer (PBS containing 0.1% bovine serum albumin) for 30 min at 4 °C followed by Alexa Fluor 647-conjugated goat Ig anti-mouse IgG antibody (Thermo Fisher Scientific, 5 μg ml−1 ). .. Unbound antibodies were removed by washing with cold PBS twice and the surface fluorescence of 104 viable cells was measured by FACScaliber flow cytometer (Becton Dickinson) and analysed with Flowjo (Tree Star Inc.).

    Labeling:

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: Cells were then stained with Hoechst 33342 for 30 min. For analysis of labeled mitosis, the Click-iT EdU Pacific Blue Flow Cytometry Assay kit (Invitrogen) was used in combination with an M-phase marker, phospho-histone H3. .. Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice.

    Article Title: Receptor-stimulated Transamidation Induces Activation of Rac1 and Cdc42 and the Regulation of Dendritic Spines
    Article Snippet: Primary neurons were double-labeled with Alexa Fluor® 568 Phalloidin (Life Technologies, Grand Island, NY) for labeling F-actin, antibodies against microtubule-associated protein 2 (Map2) clone HM-2 (Sigma-Aldrich, St. Louis, MO) for labeling dendrites, antibodies against 5-HT2A receptors , and antibodies against PSD95 (6G6-1C9) (Life Technologies, Grand Island, NY). .. Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) antibody and Alexa Fluor® 647 conjugated goat anti-mouse IgG (H+L) secondary antibody (Life Technologies, Grand Island, NY) were used to target primary antibodies.

    Purification:

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. A set of 11 overlapping peptides, each comprising 10–12 amino acids from DENV-2 domain III was synthesized and purified by HPLC.

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Yeast cells were washed with PBS containing 1 mg/ml bovine serum albumin (PBS-BSA) and incubated with 40 μl of MAb (neat supernatant or 20 μg/ml purified and diluted in PBS) for 30 min on ice. .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: Yeast cells were washed with PBS supplemented with BSA (1 mg/ml) and incubated with 50 µl of neat culture supernatant or purified MAbs at a concentration of 25 µg/ml. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    Microscopy:

    Article Title: Epigenetic Regulation Alters Biofilm Architecture and Composition in Multiple Clinical Isolates of Nontypeable Haemophilus influenzae
    Article Snippet: After 16 h, biofilms were rinsed twice with DPBS to remove nonadherent bacteria and then incubated with anti-NTHI HU polyclonal rabbit sera (1:200) for 30 min or commercially available murine antibody against dsDNA (AbCam) (1:200) for 1 h. Biofilms were then rinsed and incubated for 15 min with goat anti-rabbit or goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Thermo Fisher, Waltham, MA). .. Biofilms were rinsed with DPBS and imaged with a Zeiss 700 Meta-laser scanning confocal microscope.

    FACS:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: Fluorescence data were collected on a FACS machine (FACS Canto II, Becton-Dickinson, USA). .. To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA).

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice. .. Each sample was analyzed on a FACS CantoII cytometer (Becton Dickinson, Franklin Lakes, NJ) using the FlowJo software (Tree Star, Ashland, OR, USA).

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. After fixation with 1 % paraformaldehyde in PBS, yeast cells were analysed on a FACS Scan flow cytometer (Becton Dickinson) using FlowJo software (Tree Star).

    Plasmid Preparation:

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: DENV-2 domain III mutants that were generated as part of a random library by error-prone mutagenesis in the pYD1 vector were expressed on the surface of yeast as described previously ( ). .. After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. The libraries were ligated into the pYD1 vector and transformed into XL2-Blue ultracompetent cells (Stratagene) with ∼5.7 × 105 and 5.5 × 105 transformants for genotypes 1a and 2a, respectively.

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: The PCR products were cloned as downstream fusions to Aga2 and Xpress epitope tag genes in the yeast surface display vector pYD1 (Invitrogen), under the control of an upstream GAL1 promoter. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    Software:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: Data analysis was performed using FlowJo 10 software (Ashland, OR). .. To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA).

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice. .. Each sample was analyzed on a FACS CantoII cytometer (Becton Dickinson, Franklin Lakes, NJ) using the FlowJo software (Tree Star, Ashland, OR, USA).

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. After fixation with 1 % paraformaldehyde in PBS, yeast cells were analysed on a FACS Scan flow cytometer (Becton Dickinson) using FlowJo software (Tree Star).

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star). .. Random mutant libraries of E2 were generated from genotype 1a (strain H77) and genotype 2a (strain J6) genes by error-prone PCR using a GeneMorph II random-mutagenesis kit (Stratagene).

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes). .. After fixation with 1% paraformaldehyde in PBS, yeast cells were analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software.

    Irradiation:

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: Briefly, cells were incubated in growth medium containing 10 μM EdU (Invitrogen) for 20 min immediately after irradiation, and then washed in PBS. .. Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice.

    Selection:

    Article Title: Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step ▿ †
    Article Snippet: Individual yeast colonies were grown to logarithmic phase at 30°C in tryptophan-free yeast selection medium containing 2% glucose. .. Yeasts were washed in PBS-BSA, incubated with goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes) for 30 min on ice, washed, and analyzed on a FACSArray flow cytometer (Becton-Dickinson) using FloJo software (Tree Star).

    Concentration Assay:

    Article Title: The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1
    Article Snippet: Yeast cells were washed with PBS supplemented with BSA (1 mg/ml) and incubated with 50 µl of neat culture supernatant or purified MAbs at a concentration of 25 µg/ml. .. After a 30 minute incubation on ice, yeast were washed in PBS with BSA and then incubated with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes).

    Marker:

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: Cells were then stained with Hoechst 33342 for 30 min. For analysis of labeled mitosis, the Click-iT EdU Pacific Blue Flow Cytometry Assay kit (Invitrogen) was used in combination with an M-phase marker, phospho-histone H3. .. Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice.

    Staining:

    Article Title: Akt inhibition at the initial stage of CAR-T preparation enhances the CAR-positive expression rate, memory phenotype and in vivo efficacy
    Article Snippet: .. To determine CAR expression at the end of CAR-T cell expansion (Day 10), 2 × 105 T cells were stained, first with a mouse anti-c-myc tag monoclonal antibody (Sigma-Aldrich, USA) and, then, with an Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, USA). .. For p-Akt determination, intracellular staining was performed with FITC-conjugated anti-p-Akt (s473) (eBioscience, USA).

    Article Title: Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population
    Article Snippet: After the indicated time, cells were fixed in 4% paraformaldehyde in PBS for 15 min, and then stained using azide conjugated with Pacific Blue. .. Following extensive washing, cells were incubated in goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (1:500; Invitrogen) for 30 min on ice.

    Article Title: On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
    Article Snippet: .. After 30 min incubation on ice, yeast were washed in PBS with BSA and then stained with a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 647 (Molecular Probes, Invitrogen). .. After fixation with 1 % paraformaldehyde in PBS, yeast cells were analysed on a FACS Scan flow cytometer (Becton Dickinson) using FlowJo software (Tree Star).

    Article Title: Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
    Article Snippet: .. Flow cytometer analysis Surface expression of EGFR in human hepatocytes, PC3, SKBR3, SK-MES-1, Hut125, Caski, HT29, H2170, LS174T, HepG2, SW480, MDA-MB-231, MDA-MB-468 and BT-20 cells was determined by staining the cells with monoclonal mouse IgG anti-human EGFR (Santa Cruz Biotechnology, 5 μg ml−1 in staining buffer (PBS containing 0.1% bovine serum albumin) for 30 min at 4 °C followed by Alexa Fluor 647-conjugated goat Ig anti-mouse IgG antibody (Thermo Fisher Scientific, 5 μg ml−1 ). .. Unbound antibodies were removed by washing with cold PBS twice and the surface fluorescence of 104 viable cells was measured by FACScaliber flow cytometer (Becton Dickinson) and analysed with Flowjo (Tree Star Inc.).

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    Thermo Fisher alexa fluor 647 conjugated f
    Alexa Fluor 647 Conjugated F, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher alexa fluor conjugated secondary antibody
    Alexa Fluor Conjugated Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor conjugated secondary antibody/product/Thermo Fisher
    Average 90 stars, based on 214 article reviews
    Price from $9.99 to $1999.99
    alexa fluor conjugated secondary antibody - by Bioz Stars, 2020-01
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