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alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab  (Bio-Rad)

 
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    Bio-Rad alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab
    Complex formation between Xk and Vps13a in WR19L cells. ( A ) BN-PAGE analysis of Xk and Vps13a in WR19L cells. The solubilized crude membrane fractions (4.2 µg of protein) and the cytosolic fractions (21 µg of protein) from DKO , DKO <t>-P2X7,</t> Xk −/− DKO -P2X7 ( Xk −/− ), and Vps13a −/− DKO -P2X7 ( Vps13a −/− ) were separated by BN-PAGE or SDS-PAGE and analyzed by Western blotting with anti-Xk ( Left ) or anti-Vps13a Ab ( Right ). Each membrane was stained with CBB and shown in the Lower panels. The positions of Mr. standard proteins are shown with their Mr. ( B ) The cellular localization of Xk. Xk −/− DKO -P2X7 ( Xk −/− ) and Xk −/− Vps13a −/− DKO -P2X7 ( Xk −/− Vps13a −/− ) cells were transformed with Xk–EGFP and observed by confocal microscopy in the presence of 5 µg/mL Hoechst 33342. EGFP and Hoechst signals are shown in green and blue. (Scale bars, 10 µm.)
    Alexa 647 Conjugated Rat Anti Mouse P2x7 Monoclonal Ab Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab - by Bioz Stars, 2024-10
    93/100 stars

    Images

    1) Product Images from "Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells"

    Article Title: Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2119286119

    Complex formation between Xk and Vps13a in WR19L cells. ( A ) BN-PAGE analysis of Xk and Vps13a in WR19L cells. The solubilized crude membrane fractions (4.2 µg of protein) and the cytosolic fractions (21 µg of protein) from DKO , DKO -P2X7, Xk −/− DKO -P2X7 ( Xk −/− ), and Vps13a −/− DKO -P2X7 ( Vps13a −/− ) were separated by BN-PAGE or SDS-PAGE and analyzed by Western blotting with anti-Xk ( Left ) or anti-Vps13a Ab ( Right ). Each membrane was stained with CBB and shown in the Lower panels. The positions of Mr. standard proteins are shown with their Mr. ( B ) The cellular localization of Xk. Xk −/− DKO -P2X7 ( Xk −/− ) and Xk −/− Vps13a −/− DKO -P2X7 ( Xk −/− Vps13a −/− ) cells were transformed with Xk–EGFP and observed by confocal microscopy in the presence of 5 µg/mL Hoechst 33342. EGFP and Hoechst signals are shown in green and blue. (Scale bars, 10 µm.)
    Figure Legend Snippet: Complex formation between Xk and Vps13a in WR19L cells. ( A ) BN-PAGE analysis of Xk and Vps13a in WR19L cells. The solubilized crude membrane fractions (4.2 µg of protein) and the cytosolic fractions (21 µg of protein) from DKO , DKO -P2X7, Xk −/− DKO -P2X7 ( Xk −/− ), and Vps13a −/− DKO -P2X7 ( Vps13a −/− ) were separated by BN-PAGE or SDS-PAGE and analyzed by Western blotting with anti-Xk ( Left ) or anti-Vps13a Ab ( Right ). Each membrane was stained with CBB and shown in the Lower panels. The positions of Mr. standard proteins are shown with their Mr. ( B ) The cellular localization of Xk. Xk −/− DKO -P2X7 ( Xk −/− ) and Xk −/− Vps13a −/− DKO -P2X7 ( Xk −/− Vps13a −/− ) cells were transformed with Xk–EGFP and observed by confocal microscopy in the presence of 5 µg/mL Hoechst 33342. EGFP and Hoechst signals are shown in green and blue. (Scale bars, 10 µm.)

    Techniques Used: SDS Page, Western Blot, Staining, Transformation Assay, Confocal Microscopy



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    alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab  (Bio-Rad)
    93
    Bio-Rad alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab
    Complex formation between Xk and Vps13a in WR19L cells. ( A ) BN-PAGE analysis of Xk and Vps13a in WR19L cells. The solubilized crude membrane fractions (4.2 µg of protein) and the cytosolic fractions (21 µg of protein) from DKO , DKO <t>-P2X7,</t> Xk −/− DKO -P2X7 ( Xk −/− ), and Vps13a −/− DKO -P2X7 ( Vps13a −/− ) were separated by BN-PAGE or SDS-PAGE and analyzed by Western blotting with anti-Xk ( Left ) or anti-Vps13a Ab ( Right ). Each membrane was stained with CBB and shown in the Lower panels. The positions of Mr. standard proteins are shown with their Mr. ( B ) The cellular localization of Xk. Xk −/− DKO -P2X7 ( Xk −/− ) and Xk −/− Vps13a −/− DKO -P2X7 ( Xk −/− Vps13a −/− ) cells were transformed with Xk–EGFP and observed by confocal microscopy in the presence of 5 µg/mL Hoechst 33342. EGFP and Hoechst signals are shown in green and blue. (Scale bars, 10 µm.)
    Alexa 647 Conjugated Rat Anti Mouse P2x7 Monoclonal Ab Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa 647 conjugated rat anti mouse p2x7 monoclonal ab mab - by Bioz Stars, 2024-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Complex formation between Xk and Vps13a in WR19L cells. ( A ) BN-PAGE analysis of Xk and Vps13a in WR19L cells. The solubilized crude membrane fractions (4.2 µg of protein) and the cytosolic fractions (21 µg of protein) from DKO , DKO -P2X7, Xk −/− DKO -P2X7 ( Xk −/− ), and Vps13a −/− DKO -P2X7 ( Vps13a −/− ) were separated by BN-PAGE or SDS-PAGE and analyzed by Western blotting with anti-Xk ( Left ) or anti-Vps13a Ab ( Right ). Each membrane was stained with CBB and shown in the Lower panels. The positions of Mr. standard proteins are shown with their Mr. ( B ) The cellular localization of Xk. Xk −/− DKO -P2X7 ( Xk −/− ) and Xk −/− Vps13a −/− DKO -P2X7 ( Xk −/− Vps13a −/− ) cells were transformed with Xk–EGFP and observed by confocal microscopy in the presence of 5 µg/mL Hoechst 33342. EGFP and Hoechst signals are shown in green and blue. (Scale bars, 10 µm.)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells

    doi: 10.1073/pnas.2119286119

    Figure Lengend Snippet: Complex formation between Xk and Vps13a in WR19L cells. ( A ) BN-PAGE analysis of Xk and Vps13a in WR19L cells. The solubilized crude membrane fractions (4.2 µg of protein) and the cytosolic fractions (21 µg of protein) from DKO , DKO -P2X7, Xk −/− DKO -P2X7 ( Xk −/− ), and Vps13a −/− DKO -P2X7 ( Vps13a −/− ) were separated by BN-PAGE or SDS-PAGE and analyzed by Western blotting with anti-Xk ( Left ) or anti-Vps13a Ab ( Right ). Each membrane was stained with CBB and shown in the Lower panels. The positions of Mr. standard proteins are shown with their Mr. ( B ) The cellular localization of Xk. Xk −/− DKO -P2X7 ( Xk −/− ) and Xk −/− Vps13a −/− DKO -P2X7 ( Xk −/− Vps13a −/− ) cells were transformed with Xk–EGFP and observed by confocal microscopy in the presence of 5 µg/mL Hoechst 33342. EGFP and Hoechst signals are shown in green and blue. (Scale bars, 10 µm.)

    Article Snippet: Alexa 647-conjugated rat anti-mouse P2X7 monoclonal Ab (mAb) (clone Hano43) was from Bio-Rad Laboratories.

    Techniques: SDS Page, Western Blot, Staining, Transformation Assay, Confocal Microscopy