Journal: Nature Communications
Article Title: Interleukin-11 causes alveolar type 2 cell dysfunction and prevents alveolar regeneration
doi: 10.1038/s41467-024-52810-8
Figure Lengend Snippet: a Experimental design for the 2D culture of primary human pulmonary alveolar epithelial cells (HPAEpiC) treated with IL11 (5 ng/ml) or TGFβ1 (5 ng/ml) in the presence of anti-IL11 (X203) or IgG control antibodies (2 µg/ml). b – e Immunostaining and quantification of Fibronectin, Collagen I or KRT8 expression in HPAEpiC. n = 3/group. FC fold change. f Experimental design for the 2D culture of primary human small airway epithelial cells (HSAEC) treated with IL11 (5 ng/ml) or TGFβ1 (5 ng/ml) with anti-IL11 (X203) or IgG control antibodies (2 µg/ml). g , h Immunostaining of Fibronectin or Collagen I expression in HSAEC. n = 2/group. Quantification data were shown in Supplementary Fig. . i Experimental design and j immunostaining of Collagen I expression in Sftpc-tdT + AT2 cells treated with IL11 (5 ng/ml), TGFβ1 (5 ng/ml), X203 or IgG control antibodies (2 µg/ml). k Experimental design and l immunostaining images of Collagen I in Sftpc-tdT + AT2 cells treated with IL11 (5 ng/ml) and MEK inhibitor U1026 (10 µM). Quantification of Collagen I expression are shown in Supplementary Fig. . m Experimental design for the in vitro differentiation of Sftpc-tdT + AT2 cells to AT1 cells in the presence of IL11 (5 ng/ml), TGFβ1 (5 ng/ml), X203 or IgG control antibodies (2 µg/ml). n Immunostaining of KRT8 and PDPN in Sftpc-tdT + cells at day 5. Individual cells are highlighted within dotted lines in split channels. o Violin plots of cell size and immunostaining quantification of KRT8 or PDPN expression in tdT + cells at day 5; One representative dataset of two independent experiments are shown ( n > 50 cells/group). Data were representative of three independent experiments in ( b – d ) or two independent experiments in ( g , h , j , l , n ). Scale bars: 100 µm ( b – d , g , h ), 25 µm ( j , l ), 50 µm ( o ). Data in ( e ) are mean ± s.d. and P values determined by one-way ANOVA with Tukey’s multiple comparison test.
Article Snippet: Human small airway epithelial cells (HSAEC) (Lonza Bioscience, CC-2547) were cultured in SAGM TM small airway epithelial cell growth medium kit (Lonza Bioscience, CC-3118) and used for experiments at passage 3.
Techniques: Control, Immunostaining, Expressing, In Vitro, Comparison
