rabbit anti pan neurofascin  (Alomone Labs)

Journal: The Journal of Cell Biology

Article Title: Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments

doi: 10.1083/jcb.200109026

Figure Lengend Snippet: Na v 1.6, ankyrin-G, β IV spectrin, neurofascin, and NrCAM are targeted to axon initial segments of cerebellar Purkinje neurons. Sections of adult rat cerebellum were triple labeled with antibodies against calbindin (blue), ankyrin-G (green), and either Na v 1.6 (B), βIV spectrin (E), neurofascin (H), or NrCAM (K) (red). Composite images are shown in C, F, I, and L. Arrowheads indicate Purkinje cell initial segments. Bars, 10 μm.

Article Snippet: Antibodies used include a mouse monoclonal antibody against the ankyrin-G spectrin–binding domain ( ); affinity-purified rabbit polyclonal antibodies against neurofascin , NrCAM ( ) and the peptide CIANHTGVDIHRNGDFQKNG corresponding to residues 1042–1061 of mouse or rat Na v 1.6 (Alomone Labs); a goat polyclonal antibody against calbindin (Santa Cruz Biotech), and a chicken polyclonal antibody against the βIV spectrin unique domain that has been adsorbed against brain lysate from a βIV spectrin knockout mouse (gift of Dr. M. Komada, Tokyo Institute of Technology, Tokyo, Japan).

Techniques: Labeling

Journal: The Journal of Cell Biology

Article Title: Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments

doi: 10.1083/jcb.200109026

Figure Lengend Snippet: Targeting of β IV spectrin, Na v 1.6, NrCAM, and neurofascin to Purkinje neuron initial segments is disrupted in ankyrin-G cerebellum- specific knockout mice. Cerebellar sections from wild-type (A, D, G, J, and M) or cerebellar ankyrin-G knockout mice (B, C, E, F, H, I, K, L, N, and O) were double labeled with antibodies against calbindin (red in A–F; green in G–L) and either ankyrin-G (green in A–C), βIV spectrin (green in D–F), Na v 1.6 (red in G–I), NrCAM (red in J–L), or neurofascin (M–O). All images except M–O are composites. Bars: (A, B, and E) 5 μm; (C, D, F, G, I, and J–O) 10 μm; and (H) 25 μm.

Article Snippet: Antibodies used include a mouse monoclonal antibody against the ankyrin-G spectrin–binding domain ( ); affinity-purified rabbit polyclonal antibodies against neurofascin , NrCAM ( ) and the peptide CIANHTGVDIHRNGDFQKNG corresponding to residues 1042–1061 of mouse or rat Na v 1.6 (Alomone Labs); a goat polyclonal antibody against calbindin (Santa Cruz Biotech), and a chicken polyclonal antibody against the βIV spectrin unique domain that has been adsorbed against brain lysate from a βIV spectrin knockout mouse (gift of Dr. M. Komada, Tokyo Institute of Technology, Tokyo, Japan).

Techniques: Knock-Out, Labeling

Journal: The Journal of Cell Biology

Article Title: Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments

doi: 10.1083/jcb.200109026

Figure Lengend Snippet: Targeting of ankyrin-G and β IV spectrin to Purkinje neuron initial segments precedes Na v 1.6 and neurofascin during development. Sections of P2 rat cerebellum were triple labeled with the same antibodies as described in the legend to . D-I show initial segments containing punctate Na v 1.6 (E) or neurofascin (H). D′–I′ show initial segments that do not contain Na v 1.6 (E′) or neurofascin (H′). Arrowheads mark Purkinje neuron axon initial segments. Bars, 10 μm.

Article Snippet: Antibodies used include a mouse monoclonal antibody against the ankyrin-G spectrin–binding domain ( ); affinity-purified rabbit polyclonal antibodies against neurofascin , NrCAM ( ) and the peptide CIANHTGVDIHRNGDFQKNG corresponding to residues 1042–1061 of mouse or rat Na v 1.6 (Alomone Labs); a goat polyclonal antibody against calbindin (Santa Cruz Biotech), and a chicken polyclonal antibody against the βIV spectrin unique domain that has been adsorbed against brain lysate from a βIV spectrin knockout mouse (gift of Dr. M. Komada, Tokyo Institute of Technology, Tokyo, Japan).

Techniques: Labeling

Journal: The Journal of Cell Biology

Article Title: Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments

doi: 10.1083/jcb.200109026

Figure Lengend Snippet: Na v 1.6 and neurofascin are clustered throughout the entire axon initial segment of Purkinje neurons by postnatal day 9. Sections of P9 rat cerebellum were triple labeled with the same antibodies as described in the legend to . Arrowheads indicate Purkinje cell initial segments. Bars, 10 μm.

Article Snippet: Antibodies used include a mouse monoclonal antibody against the ankyrin-G spectrin–binding domain ( ); affinity-purified rabbit polyclonal antibodies against neurofascin , NrCAM ( ) and the peptide CIANHTGVDIHRNGDFQKNG corresponding to residues 1042–1061 of mouse or rat Na v 1.6 (Alomone Labs); a goat polyclonal antibody against calbindin (Santa Cruz Biotech), and a chicken polyclonal antibody against the βIV spectrin unique domain that has been adsorbed against brain lysate from a βIV spectrin knockout mouse (gift of Dr. M. Komada, Tokyo Institute of Technology, Tokyo, Japan).

Techniques: Labeling