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Compatibility of <t>Alhydrogel™</t> (AH)-adjuvanted recombinant t-NRRV antigens (P[4], P[6], P[8]) with t-sIPV antigens (types 1, 2, and 3; no adjuvant) in a mock bivalent combination vaccine formulation (t-NRRV + t-sIPV). Panel ( A ) is schematic representation of the simple mixing together of AH-adsorbed t-NRRV antigens with t-sIPV (no adjuvant) antigens to prepare a bivalent vaccine formulation. Alum adsorption and antibody binding (relative antigen concentration normalized to 100% for each antigen) results are shown for ( B , C ) AH-adjuvanted t-NRRV, ( D , E ) a mock t-sIPV formulation, and ( F , G ) bivalent vaccine formulation. Panel ( H ) is schematic representation of a mitigation strategy (using sIPV bulks dialyzed in PBS to lower the phosphate buffer concentration) showing the simple mixing together of AH-adsorbed t-NRRV antigens with t-sIPV (no adjuvant) antigens to prepare a bivalent vaccine formulation. Alum adsorption and antibody binding results are shown for ( I , J ) AH-adjuvanted t-NRRV, ( K , L ) a mock t-sIPV formulation prepared using dialyzed viral bulks, and ( M , N ) bivalent vaccine formulation in PBS. Antibody binding and AH-adsorption for all antigens were measured by the competitive ELISA assays described in . (*) no adsorption since AH is not present in the mock t-sIPV formulation. Red dashed line represents 100% adsorption, or 100% reference/relative antigen concentration. Data are presented as the mean ± range (n = 2) for AH adsorption and mean ± SD (n = 4) for antibody binding. When antigen adsorption to alum values was 100%, a range of ±10% was assigned based on the estimated LOQ of assay.
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Compatibility of Alhydrogel™ (AH)-adjuvanted recombinant t-NRRV antigens (P[4], P[6], P[8]) with t-sIPV antigens (types 1, 2, and 3; no adjuvant) in a mock bivalent combination vaccine formulation (t-NRRV + t-sIPV). Panel ( A ) is schematic representation of the simple mixing together of AH-adsorbed t-NRRV antigens with t-sIPV (no adjuvant) antigens to prepare a bivalent vaccine formulation. Alum adsorption and antibody binding (relative antigen concentration normalized to 100% for each antigen) results are shown for ( B , C ) AH-adjuvanted t-NRRV, ( D , E ) a mock t-sIPV formulation, and ( F , G ) bivalent vaccine formulation. Panel ( H ) is schematic representation of a mitigation strategy (using sIPV bulks dialyzed in PBS to lower the phosphate buffer concentration) showing the simple mixing together of AH-adsorbed t-NRRV antigens with t-sIPV (no adjuvant) antigens to prepare a bivalent vaccine formulation. Alum adsorption and antibody binding results are shown for ( I , J ) AH-adjuvanted t-NRRV, ( K , L ) a mock t-sIPV formulation prepared using dialyzed viral bulks, and ( M , N ) bivalent vaccine formulation in PBS. Antibody binding and AH-adsorption for all antigens were measured by the competitive ELISA assays described in . (*) no adsorption since AH is not present in the mock t-sIPV formulation. Red dashed line represents 100% adsorption, or 100% reference/relative antigen concentration. Data are presented as the mean ± range (n = 2) for AH adsorption and mean ± SD (n = 4) for antibody binding. When antigen adsorption to alum values was 100%, a range of ±10% was assigned based on the estimated LOQ of assay.

Journal: Vaccines

Article Title: Evaluating the Compatibility of Three Aluminum Salt-Adjuvanted Recombinant Protein Antigens (Trivalent NRRV) Combined with a Mock Trivalent Sabin-IPV Vaccine: Analytical and Formulation Challenges

doi: 10.3390/vaccines12101102

Figure Lengend Snippet: Compatibility of Alhydrogel™ (AH)-adjuvanted recombinant t-NRRV antigens (P[4], P[6], P[8]) with t-sIPV antigens (types 1, 2, and 3; no adjuvant) in a mock bivalent combination vaccine formulation (t-NRRV + t-sIPV). Panel ( A ) is schematic representation of the simple mixing together of AH-adsorbed t-NRRV antigens with t-sIPV (no adjuvant) antigens to prepare a bivalent vaccine formulation. Alum adsorption and antibody binding (relative antigen concentration normalized to 100% for each antigen) results are shown for ( B , C ) AH-adjuvanted t-NRRV, ( D , E ) a mock t-sIPV formulation, and ( F , G ) bivalent vaccine formulation. Panel ( H ) is schematic representation of a mitigation strategy (using sIPV bulks dialyzed in PBS to lower the phosphate buffer concentration) showing the simple mixing together of AH-adsorbed t-NRRV antigens with t-sIPV (no adjuvant) antigens to prepare a bivalent vaccine formulation. Alum adsorption and antibody binding results are shown for ( I , J ) AH-adjuvanted t-NRRV, ( K , L ) a mock t-sIPV formulation prepared using dialyzed viral bulks, and ( M , N ) bivalent vaccine formulation in PBS. Antibody binding and AH-adsorption for all antigens were measured by the competitive ELISA assays described in . (*) no adsorption since AH is not present in the mock t-sIPV formulation. Red dashed line represents 100% adsorption, or 100% reference/relative antigen concentration. Data are presented as the mean ± range (n = 2) for AH adsorption and mean ± SD (n = 4) for antibody binding. When antigen adsorption to alum values was 100%, a range of ±10% was assigned based on the estimated LOQ of assay.

Article Snippet: Alhydrogel ® (AH) adjuvant was purchased from InvivoGen (San Diego, CA, USA).

Techniques: Recombinant, Adjuvant, Formulation, Adsorption, Binding Assay, Concentration Assay, Competitive ELISA