Journal: EMBO Reports
Article Title: Histone modifications and Sp1 promote GPR160 expression in bone cancer pain within rodent models
doi: 10.1038/s44319-024-00292-6
Figure Lengend Snippet: Reagents and tools table
Article Snippet: Afterwards, they were subjected to overnight incubation at a temperature of 4 °C with the following primary antibodies: rabbit anti-GPR160 (1:200, Alomone Labs, AGR-090), mouse anti-NeuN (1:500, Novus, NBP1-92693), mouse anti-glutamine synthetase (GS, 1:400, Abcam, ab64613), mouse anti-CGRP (1:300, Sigma-Aldrich, C7113), isolectin B4 (IB4, 1:300, Sigma-Aldrich, L2140), mouse anti-NF200 (1:500, Sigma-Aldrich, N5389), mouse anti-H3K27me3 (1:300, Abcam, ab6002), mouse anti-H3K27ac (1:300, Thermo Scientific, MA5-42545), rabbit anti-Sp1 (1:200; Proteintech, 66508-1-Ig).
Techniques: Cell Culture, Recombinant, Sequencing, Blocking Assay, Reporter Assay, Software, RNAscope, shRNA, Chromatin Immunoprecipitation