agr030  (Alomone Labs)

Journal: Veterinary Sciences

Article Title: Expression of GnRH, Kisspeptin, and Their Specific Receptors in the Ovary and Uterus in Deslorelin-Treated Late-Prepubertal Bitches †

doi: 10.3390/vetsci11120591

Figure Lengend Snippet: List of antibodies and dilutions used in immunohistochemical staining.

Article Snippet: GnRHR , AGR030 (Alomone Labs, Jerusalem, Israel) , Rabbit polyclonal , 1:100.

Techniques: Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Gonadotropin-Releasing Hormone in Regulation of Thymic Development in Rats: Profile of Thymic Cytokines

doi: 10.3390/ijms20164033

Figure Lengend Snippet: Ontogenetic pattern of the gonadotropin-releasing hormone (GnRH) receptor expression in the developing thymus. ( A ) mRNA of GnRH receptor revealed by RT-PCR in the rat thymus on embryonic days (ED) 16, 17, 18, 19, 21 and postnatal day 3 (PND3). ( B ) Western blot assay of GnRH receptor in the rat thymus on ED17, ED18, ED19, ED20, ED21, and PND3. The anterior pituitary (PND3) was used as a positive control (+). ( C ) Protein expression of GnRH receptor in thymocytes and thymic stromal elements on ED18. Plots represent the optic density of the corresponding bands. Bars indicate the means ± SEM of three independent experiments; * p < 0.05 using one-way ANOVA test.

Article Snippet: Separated proteins were transferred to a nitrocellulose membrane in transfer buffer (25 mM Tris-HCl, pH 7.5, 192 mM glycine, 20% ethanol) and blots were incubated overnight at 4 °C with antibodies to GnRH receptor (1:1000, Alomone Labs, Jerusalem, Israel) or actin (1:10,000, Sigma, St. Louis, Mo, USA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Gonadotropin-Releasing Hormone in Regulation of Thymic Development in Rats: Profile of Thymic Cytokines

doi: 10.3390/ijms20164033

Figure Lengend Snippet: Schematic representation of GnRH effects on the thymus development in fetal and early postnatal rats. During prenatal development up to ED20–21, before the establishment of the HPG endocrine regulations, hypothalamic GnRH can be released to general circulation and provide a direct effect on the morphogenesis of thymus. Since the development of blood–brain barrier and the establishment of the HPG axis in early postnatal life GnRH is involved in the bidirectional programming of both neuroendocrine and immune functions via gonadotropins and sex steroids. In addition to the neuroendocrine regulation, the effects of GnRH synthesized in thymus can be realized by autocrine/paracrine mechanisms. The developmental pattern of the GnRH receptor expression in the thymus is in favor of this assumption. ED—embryonic day, PND—postnatal day, HP—hypophysis.

Article Snippet: Separated proteins were transferred to a nitrocellulose membrane in transfer buffer (25 mM Tris-HCl, pH 7.5, 192 mM glycine, 20% ethanol) and blots were incubated overnight at 4 °C with antibodies to GnRH receptor (1:1000, Alomone Labs, Jerusalem, Israel) or actin (1:10,000, Sigma, St. Louis, Mo, USA).

Techniques: Synthesized, Expressing