agincourt ampure xp beads  (Beckman Coulter)


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    Beckman Coulter agincourt ampure xp beads
    Agincourt Ampure Xp Beads, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agincourt ampure xp beads/product/Beckman Coulter
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    agincourt ampure xp beads - by Bioz Stars, 2020-02
    91/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    Article Snippet: Restriction digest products ranging from 5,616 to 6,117 bp were isolated by agarose gel electrophoresis and purified using a QIAquick gel extraction kit (Qiagen). .. The repaired DNA was recovered using Agincourt AMPure XP beads (A63880, Beckman Coulter), washed twice in 200 μl 70% ethanol and eluted in 46 μl dH2 O. DNA was then dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs) according to the manufacturer's instructions and recovered using Agincourt AMPure XP beads as before, eluting in 30 μl dH2 O.

    Amplification:

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis. .. Suitable cDNA fragments were selected as templates for PCR amplification using KAPA Library Amplification Primer Mix and KAPA HiFi HotStart Ready Mix.

    RNA Sequencing Assay:

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: Paragraph title: 4.8. RNA Sequencing, Annotation and Analysis ... Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis.

    Synthesized:

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: Double-stranded cDNAs were synthesized using fragmented-primed mRNA, KAPA 1st Strand Synthesis Buffer, and KAPA Script, followed by KAPA 2nd Strand Marking Buffer and KAPA 2nd Strand Synthesis Enzyme Mix. .. Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis.

    Isolation:

    Article Title: Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    Article Snippet: Restriction digest products ranging from 5,616 to 6,117 bp were isolated by agarose gel electrophoresis and purified using a QIAquick gel extraction kit (Qiagen). .. The repaired DNA was recovered using Agincourt AMPure XP beads (A63880, Beckman Coulter), washed twice in 200 μl 70% ethanol and eluted in 46 μl dH2 O. DNA was then dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs) according to the manufacturer's instructions and recovered using Agincourt AMPure XP beads as before, eluting in 30 μl dH2 O.

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: Briefly, for total RNA, mRNA isolation, fragmentation and priming were performed using a KAPA mRNA capture system (Box1). .. Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis.

    Spectrophotometry:

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: The concentration and purity of the total RNA were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis.

    Purification:

    Article Title: Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    Article Snippet: Restriction digest products ranging from 2,062 to 2,229, 2,549 to 2,716 and 1,885 to 2,052 bp for the pcbC , pclA and penDE assemblies, respectively, were purified. .. The repaired DNA was recovered using Agincourt AMPure XP beads (A63880, Beckman Coulter), washed twice in 200 μl 70% ethanol and eluted in 46 μl dH2 O. DNA was then dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs) according to the manufacturer's instructions and recovered using Agincourt AMPure XP beads as before, eluting in 30 μl dH2 O.

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: .. Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis. .. After A-tailing, Illumina adapter oligonucleotides were ligated to cDNA fragments, and 1× SPRI clean-up was performed.

    Concentration Assay:

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: The concentration and purity of the total RNA were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    Article Snippet: The four samples were combined to a give an equimolar mix (assuming a molecular weight for each assembly based on the mean promoter length) with a total DNA content of 2.6 μg in 45 μl dH2 O. DNA underwent end repair using NEBNext FFPE DNA Repair Mix (M6630, New England Biolabs) according to the manufacturer's instructions. .. The repaired DNA was recovered using Agincourt AMPure XP beads (A63880, Beckman Coulter), washed twice in 200 μl 70% ethanol and eluted in 46 μl dH2 O. DNA was then dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs) according to the manufacturer's instructions and recovered using Agincourt AMPure XP beads as before, eluting in 30 μl dH2 O.

    Polymerase Chain Reaction:

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis. .. Suitable cDNA fragments were selected as templates for PCR amplification using KAPA Library Amplification Primer Mix and KAPA HiFi HotStart Ready Mix.

    Gel Extraction:

    Article Title: Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    Article Snippet: Restriction digest products ranging from 5,616 to 6,117 bp were isolated by agarose gel electrophoresis and purified using a QIAquick gel extraction kit (Qiagen). .. The repaired DNA was recovered using Agincourt AMPure XP beads (A63880, Beckman Coulter), washed twice in 200 μl 70% ethanol and eluted in 46 μl dH2 O. DNA was then dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs) according to the manufacturer's instructions and recovered using Agincourt AMPure XP beads as before, eluting in 30 μl dH2 O.

    Chromatin Immunoprecipitation:

    Article Title: Morphological and Transcriptomic Analysis of the Inhibitory Effects of Lactobacillus plantarum on Aspergillus flavus Growth and Aflatoxin Production
    Article Snippet: Products were purified with 1.8× Agincourt AMPure XP beads (Beckman Coulter, Beverly, Brea, FL, USA) followed by 2nd strand synthesis. .. Products were purified using the AMPure XP bead system and quantified using a High Sensitivity Chip Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

    Molecular Weight:

    Article Title: Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    Article Snippet: The four samples were combined to a give an equimolar mix (assuming a molecular weight for each assembly based on the mean promoter length) with a total DNA content of 2.6 μg in 45 μl dH2 O. DNA underwent end repair using NEBNext FFPE DNA Repair Mix (M6630, New England Biolabs) according to the manufacturer's instructions. .. The repaired DNA was recovered using Agincourt AMPure XP beads (A63880, Beckman Coulter), washed twice in 200 μl 70% ethanol and eluted in 46 μl dH2 O. DNA was then dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs) according to the manufacturer's instructions and recovered using Agincourt AMPure XP beads as before, eluting in 30 μl dH2 O.

    Nanopore Sequencing:

    Article Title: Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    Article Snippet: Paragraph title: Nanopore sequencing of library construction ... The repaired DNA was recovered using Agincourt AMPure XP beads (A63880, Beckman Coulter), washed twice in 200 μl 70% ethanol and eluted in 46 μl dH2 O. DNA was then dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs) according to the manufacturer's instructions and recovered using Agincourt AMPure XP beads as before, eluting in 30 μl dH2 O.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
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  • 91
    Beckman Coulter agincourt ampure xp beads
    Agincourt Ampure Xp Beads, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 91/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agincourt ampure xp beads/product/Beckman Coulter
    Average 91 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    agincourt ampure xp beads - by Bioz Stars, 2020-02
    91/100 stars
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