agei saci  (New England Biolabs)


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    Structured Review

    New England Biolabs agei saci
    Agei Saci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agei saci/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agei saci - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    paav-cb6-pi
    paavsc-cb6-pi plasmids
    ecori/kpni

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    Related Articles

    Clone Assay:

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
    Article Snippet: The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes. .. The pCMV6-XL5-SMN plasmid was digested with NotI enzyme (New England Biolabs, MA, USA) to extract SMN cDNA with its UTRs from the original plasmid to be cloned into the pAAV-CB6-PI.

    Agarose Gel Electrophoresis:

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
    Article Snippet: The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes. .. All of the digested plasmids were run on the 1% agarose gel and 4393 bp of pAAV-CB6-PI, 4152 bp of pAAVsc-CB6-PI, 1629 bp of SMN cDNA with UTRs sequences, and 1037 bp of SMN cDNA without its UTRs were purified from the agarose gel using QIAquick gel extraction kit (Qiagen, Boston, MA, USA).

    Ligation:

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
    Article Snippet: The blunt-end ligation strategy was performed for construction of AAV Cis-plasmids carrying the SMN gene. .. The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes.

    Subcloning:

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
    Article Snippet: Paragraph title: Subcloning of Human SMN gene in pAAV-CB6-PI and pAAVsc-CB6-PI ... The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes.

    Purification:

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
    Article Snippet: The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes. .. All of the digested plasmids were run on the 1% agarose gel and 4393 bp of pAAV-CB6-PI, 4152 bp of pAAVsc-CB6-PI, 1629 bp of SMN cDNA with UTRs sequences, and 1037 bp of SMN cDNA without its UTRs were purified from the agarose gel using QIAquick gel extraction kit (Qiagen, Boston, MA, USA).

    Gel Extraction:

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
    Article Snippet: The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes. .. All of the digested plasmids were run on the 1% agarose gel and 4393 bp of pAAV-CB6-PI, 4152 bp of pAAVsc-CB6-PI, 1629 bp of SMN cDNA with UTRs sequences, and 1037 bp of SMN cDNA without its UTRs were purified from the agarose gel using QIAquick gel extraction kit (Qiagen, Boston, MA, USA).

    Plasmid Preparation:

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
    Article Snippet: The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes. .. The pCMV6-XL5-SMN plasmid was digested with NotI enzyme (New England Biolabs, MA, USA) to extract SMN cDNA with its UTRs from the original plasmid to be cloned into the pAAV-CB6-PI.

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    New England Biolabs 1 025 bp saci agei fragment
    1 025 Bp Saci Agei Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 025 bp saci agei fragment/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1 025 bp saci agei fragment - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    92
    New England Biolabs agei saci
    Agei Saci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agei saci/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agei saci - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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