agei bspei  (New England Biolabs)


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    Structured Review

    New England Biolabs agei bspei
    Agei Bspei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agei bspei/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agei bspei - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains
    Article Snippet: Plasmid construction Full-length NXF1 and Y14 sequences were PCR amplified and cloned into peYFP-C1 (CLONTECH Laboratories) using EcoRI–BamHI (New England BioLabs, Inc.) restriction sites. .. These fragments were PCR amplified and inserted using AgeI–XhoI or AgeI–BspEI (New England BioLabs, Inc.) restriction enzymes to generate fusion proteins with the NH2 - or COOH-terminal parts of YFP (YN-NXF1, YN-Y14, YC-NXF1, and YC-Y14, respectively).

    Transfection:

    Article Title: In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains
    Article Snippet: These fragments were PCR amplified and inserted using AgeI–XhoI or AgeI–BspEI (New England BioLabs, Inc.) restriction enzymes to generate fusion proteins with the NH2 - or COOH-terminal parts of YFP (YN-NXF1, YN-Y14, YC-NXF1, and YC-Y14, respectively). .. Single transfection of the plasmids used for BiFC (YN-x and YC-x) did not produce fluorescence, indicating that the vectors did not code for full-length YFP.

    Amplification:

    Article Title: In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains
    Article Snippet: .. These fragments were PCR amplified and inserted using AgeI–XhoI or AgeI–BspEI (New England BioLabs, Inc.) restriction enzymes to generate fusion proteins with the NH2 - or COOH-terminal parts of YFP (YN-NXF1, YN-Y14, YC-NXF1, and YC-Y14, respectively). .. NXF1 deletion mutants (YN-NXF1 Δ1–60 and YN-NXF1 Δ1–371) were linked to full-length YFP or to the NH2 -terminal part of YFP using EcoRI–BamHI.

    Fluorescence:

    Article Title: In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains
    Article Snippet: These fragments were PCR amplified and inserted using AgeI–XhoI or AgeI–BspEI (New England BioLabs, Inc.) restriction enzymes to generate fusion proteins with the NH2 - or COOH-terminal parts of YFP (YN-NXF1, YN-Y14, YC-NXF1, and YC-Y14, respectively). .. Single transfection of the plasmids used for BiFC (YN-x and YC-x) did not produce fluorescence, indicating that the vectors did not code for full-length YFP.

    Bimolecular Fluorescence Complementation Assay:

    Article Title: In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains
    Article Snippet: These fragments were PCR amplified and inserted using AgeI–XhoI or AgeI–BspEI (New England BioLabs, Inc.) restriction enzymes to generate fusion proteins with the NH2 - or COOH-terminal parts of YFP (YN-NXF1, YN-Y14, YC-NXF1, and YC-Y14, respectively). .. Single transfection of the plasmids used for BiFC (YN-x and YC-x) did not produce fluorescence, indicating that the vectors did not code for full-length YFP.

    Polymerase Chain Reaction:

    Article Title: In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains
    Article Snippet: .. These fragments were PCR amplified and inserted using AgeI–XhoI or AgeI–BspEI (New England BioLabs, Inc.) restriction enzymes to generate fusion proteins with the NH2 - or COOH-terminal parts of YFP (YN-NXF1, YN-Y14, YC-NXF1, and YC-Y14, respectively). .. NXF1 deletion mutants (YN-NXF1 Δ1–60 and YN-NXF1 Δ1–371) were linked to full-length YFP or to the NH2 -terminal part of YFP using EcoRI–BamHI.

    Plasmid Preparation:

    Article Title: In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains
    Article Snippet: Paragraph title: Plasmid construction ... These fragments were PCR amplified and inserted using AgeI–XhoI or AgeI–BspEI (New England BioLabs, Inc.) restriction enzymes to generate fusion proteins with the NH2 - or COOH-terminal parts of YFP (YN-NXF1, YN-Y14, YC-NXF1, and YC-Y14, respectively).

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  • News
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  • Team
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  • 86
    New England Biolabs agei bspei
    Agei Bspei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agei bspei/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agei bspei - by Bioz Stars, 2020-04
    86/100 stars
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