age1 restriction enzymes  (New England Biolabs)


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    Structured Review

    New England Biolabs age1 restriction enzymes
    Age1 Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/age1 restriction enzymes/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    age1 restriction enzymes - by Bioz Stars, 2019-10
    91/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs). .. Obtained transformants were analyzed by PCR and automated sequencing.

    Amplification:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs). .. Obtained transformants were analyzed by PCR and automated sequencing.

    In Vitro:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs). .. Obtained transformants were analyzed by PCR and automated sequencing.

    TA Cloning:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs).

    Construct:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs).

    Purification:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs). .. Obtained transformants were analyzed by PCR and automated sequencing.

    Polymerase Chain Reaction:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs). .. Obtained transformants were analyzed by PCR and automated sequencing.

    DNA Sequencing:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: After PCR analysis of approximately 200 obtained transformants, six clones (A, H, S, U, W, Y) were selected based upon PCR amplicon size/abundance (ranging between 300 and 2100 base pairs approximately), and analyzed by automated sequencing to confirm insert size, sequence and orientation (UAMS DNA Sequencing Core Facility). .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs).

    Expressing:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs).

    Sequencing:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs).

    Transformation Assay:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs).

    Hemagglutination Assay:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs).

    Plasmid Preparation:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All constructs were designed, based upon clone sequence homology, so as to insert: 1) HA peptide coding sequence between the 24th and 25th base pairs (bp) of rat Mesothelin consensus coding sequence (NCBI Nucleotide ID: NM_031658.1 [ – ]), resulting in a 9-amino acid insertion between the 8th and 9th amino acids of rat Mesothelin protein sequence (NCBI Protein ID: NP_113846 [ – ]); and 2) Xba1 and Age1 restriction sites at both 5’- (before ATG start codon, NM_031658.1 [ ]) and 3’-ends (after TGA stop codon, NM_031658.1 [625]) of rat Mesothelin coding sequence. .. All PCR amplicons were purified and used for: 1) adaptor sequences addition by PCR amplification, followed by in vitro translation reactions using the cell-free PURExpress® In Vitro Protein Synthesis Kit (New England BioLabs); and 2) directional cloning into the pcDNA™ 3.3 TOPO® TA vector using a Gibson Assembly kit (New England BioLabs), and Xba1 and Age1 restriction enzymes (New England BioLabs). .. Obtained transformants were analyzed by PCR and automated sequencing.

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    New England Biolabs agei hf
    Agei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Age1 Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/age1 restriction enzymes/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    New England Biolabs restriction enzymes age1
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    https://www.bioz.com/result/restriction enzymes age1/product/New England Biolabs
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