anti-mglur8 antibody  (Alomone Labs)

Journal: Bioengineering

Article Title: Targeting Ion Channels: Blockers Suppress Calcium Signals and Induce Cytotoxicity Across Medulloblastoma Cell Models

doi: 10.3390/bioengineering12030268

Figure Lengend Snippet: Relative ion channel gene expression in MB primary tumors and cultures. The heatmap illustrates the relative expression levels of ion channel genes identified in the literature as dysregulated in MB. Red denotes higher levels of gene expression, and blue represents lower levels. Tumor types are indicated along the top x-axis, with blue labels corresponding to primary medulloblastoma (MB) tumors and green labels representing cultures derived from primary MB tissue. Both primary MB tumors and their derived cultures exhibit upregulated expression of potassium channel genes, including KCNB2, KCNA5, KCNA1, KCNJ2, GRK4, and KCNB1 (along the right y-axis). In contrast, astrocytoma, ependymoma, and their associated cultures show relatively lower expression levels of these ion channels.

Article Snippet: Primary ion channel antibodies were from Alomone Labs (Jerusalem, Israel): mGluR8 (rabbit polyclonal, extracellular, Cat# AGC-028), Kv1.1 (rabbit polyclonal, extracellular, Cat# APC-161), Kv1.5 (rabbit polyclonal, intracellular, Cat# APC-004), Kv2.1 (rabbit polyclonal, intracellular, Cat# APC-012), Kv2.2 (rabbit polyclonal, intracellular, Cat# APC-120), Kir2.1 (rabbit polyclonal, intracellular, Cat# APC-026), and sodium channels (Pan-NaV) (rabbit polyclonal, intracellular, Cat# ASC-003).

Techniques: Gene Expression, Expressing, Derivative Assay

Journal: Bioengineering

Article Title: Targeting Ion Channels: Blockers Suppress Calcium Signals and Induce Cytotoxicity Across Medulloblastoma Cell Models

doi: 10.3390/bioengineering12030268

Figure Lengend Snippet: Ion channel expression in medulloblastoma (MB) primary culture. ( A ). Representative immunostaining images showing the expression of channel proteins, including mGluR8 (a,b), Kv1.5 (c,d), Kv2.2 (e,f), Kir2.1 (g,h), and Pan-NaV (i,j). Channel proteins are visualized in red and counterstained with DAPI (blue) for nuclear staining. Overlays of phase-contrast images (a,c,e,g,i) demonstrate the spindly morphology of MB cells in culture. White arrows in e and f indicate Kv2.2 localization at sites where neurite-like processes originate. Scale bar, 100 um. ( B ). Flow cytometry analysis histograms of cells derived from a patient with MB depicting positive expression for specific channel proteins: mGluR8 (a), Kv1.5 (b), Kv2.2 (c), Kir2.1 (d) and Pan-NaV (e). Percentages indicate the proportion of cells in the positive gate, with gating thresholds established using secondary antibody-only controls (no primary antibody staining).

Article Snippet: Primary ion channel antibodies were from Alomone Labs (Jerusalem, Israel): mGluR8 (rabbit polyclonal, extracellular, Cat# AGC-028), Kv1.1 (rabbit polyclonal, extracellular, Cat# APC-161), Kv1.5 (rabbit polyclonal, intracellular, Cat# APC-004), Kv2.1 (rabbit polyclonal, intracellular, Cat# APC-012), Kv2.2 (rabbit polyclonal, intracellular, Cat# APC-120), Kir2.1 (rabbit polyclonal, intracellular, Cat# APC-026), and sodium channels (Pan-NaV) (rabbit polyclonal, intracellular, Cat# ASC-003).

Techniques: Expressing, Immunostaining, Staining, Flow Cytometry, Derivative Assay

Journal: FEBS Open Bio

Article Title: Hippocampal tau‐induced GRIN3A deficiency in Alzheimer's disease

doi: 10.1002/2211-5463.13904

Figure Lengend Snippet: Experimental validation of tau‐induced NR3A deficiency by using human iPSC‐derived brain organoids. (A) Representative images of paired helical filament (PHF)‐tau‐treated brain organoids. The organoids were labeled for MAP2 (green), PHF‐tau (magenta), and DAPI (blue). Scale bar = 10 μm. (B) Representative 3D view images of IMARIS 3D rendering to compare fractions of NR3A or PSD95 overlapped with each other. The organoids were labeled for MAP2 (green), NR3A (red), PSD95 (magenta), and DAPI (blue). White arrows show the positions colocalized between PSD95 and NR3A. The dotted circles show the magnified positions. n = 4 slides for each group. Scale bar = 10 or 3 μm. (C) Representative 3D view images of imaris 3D rendering to compare fractions of mGluR8 or PSD95 that overlapped with each other. The organoids were labeled for MAP2 (green), mGluR8 (red), PSD95 (magenta), and DAPI (blue). White arrows show the positions colocalized between PSD95 and mGluR8. The dotted circles show the magnified positions. n = 4 slides for each group. Scale bar = 10 or 3 μm. (D) Quantification data of (B) and (C). P ‐values by Mann–Whitney test (* P < 0.05). n = 4 slides for each group. (E) Western blot analysis to compare the total levels of NR3A between PHF‐tau‐treated organoids and vehicles. P ‐values by Welch's t ‐test ( # P = 0.1).

Article Snippet: Primary antibodies used in this study are anti‐NMDAR3A (rabbit polyclonal, Alomone, AGC‐030, Jerusalem, Israel), anti‐mGluR8 (rabbit polyclonal, Alomone, AGC‐028), anti‐PSD95 (mouse monoclonal, Invitrogen, Carlsbad, CA, USA, MA1‐045), anti‐GAPDH (rabbit polyclonal, Abcam, ab9485, Cambridge, UK), anti‐MAP2 (goat polyclonal, Phosphosolutions, 1099‐MAP2t, Aurora, CO, USA), anti‐Phospho‐Tau (Thr181) (mouse monoclonal, ThermoFisher, MN1050, Waltham, MA, USA) and DAPI (Sigma‐Aldrich, D9542, Saint Louis, MO, USA), Alexa Fluor™‐647/568/405 labeled secondary antibodies were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Derivative Assay, Labeling, MANN-WHITNEY, Western Blot