Review



glua3  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Alomone Labs glua3
    Nutritional state differentially modulates synaptic transmission in WT and OGT-KO neurons. A , representative trace showing suppression of spontaneous excitatory synaptic events following NBQX application in WT PVN αCaMKII neurons. The red arrow represents a typical excitatory spike. B – D , representative Western blots and quantification of total and surface GluA1 expression in primary cortical neurons. WT values were normalized to 1. OGT-KO total GluA1 = 1.282 ± 0.068; surface GluA1 = 1.629 ± 0.064. E–G , representative Western blots and quantification of total and surface GluA2 expression. WT values were normalized to 1. OGT-KO total GluA2 = 0.7101 ± 0.081; surface GluA2 = 0.5138 ± 0.075. H–J , representative Western blots and quantification of total and surface <t>GluA3</t> expression. WT values were normalized to 1. OGT-KO total GluA3 = 0.6691 ± 0.032; surface GluA3 = 0.3935 ± 0.044. Western blot quantitative analyses were performed using the Wilcoxon signed-rank test. All Western blot data expressed as mean ± SEM. K , representative sEPSC traces in WT neurons under hungry ( top ) and fed ( bottom ) conditions. L , sEPSC frequency in WT neurons comparing hungry vs fed states ( p = 0.021). M , sEPSC amplitude in WT neurons comparing hungry vs fed states (NS, p = 0.851). N , sEPSC decay tau in WT neurons comparing hungry vs fed states (NS, p = 0.142). O , Representative sEPSC traces in OGT-KO neurons under hungry ( top ) and fed ( bottom ) conditions. P , sEPSC frequency in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.095). Q , sEPSC amplitude in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.309). R , sEPSC decay tau in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.547). All sEPSC data ( panels K–R ) were obtained from WT (n = 5) and OGT-KO (n = 5) mice under hungry and fed conditions (n = 5 each condition). Statistical comparisons using Mann-Whitney non-parametric test. Data presented as mean ± SEM. Blue bars: WT neurons ( dark blue = hungry, light blue = fed); yellow bars: OGT-KO neurons ( light yellow = hungry, bright yellow = fed). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, NS, non-significant.
    Glua3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agc-010-gp/pmc12870757-267-37-38?v=Alomone+Labs
    Average 94 stars, based on 2 article reviews
    glua3 - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "O-GlcNAc transferase couples nutrient availability to synaptic plasticity in paraventricular neurons to regulate satiety"

    Article Title: O-GlcNAc transferase couples nutrient availability to synaptic plasticity in paraventricular neurons to regulate satiety

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.111124

    Nutritional state differentially modulates synaptic transmission in WT and OGT-KO neurons. A , representative trace showing suppression of spontaneous excitatory synaptic events following NBQX application in WT PVN αCaMKII neurons. The red arrow represents a typical excitatory spike. B – D , representative Western blots and quantification of total and surface GluA1 expression in primary cortical neurons. WT values were normalized to 1. OGT-KO total GluA1 = 1.282 ± 0.068; surface GluA1 = 1.629 ± 0.064. E–G , representative Western blots and quantification of total and surface GluA2 expression. WT values were normalized to 1. OGT-KO total GluA2 = 0.7101 ± 0.081; surface GluA2 = 0.5138 ± 0.075. H–J , representative Western blots and quantification of total and surface GluA3 expression. WT values were normalized to 1. OGT-KO total GluA3 = 0.6691 ± 0.032; surface GluA3 = 0.3935 ± 0.044. Western blot quantitative analyses were performed using the Wilcoxon signed-rank test. All Western blot data expressed as mean ± SEM. K , representative sEPSC traces in WT neurons under hungry ( top ) and fed ( bottom ) conditions. L , sEPSC frequency in WT neurons comparing hungry vs fed states ( p = 0.021). M , sEPSC amplitude in WT neurons comparing hungry vs fed states (NS, p = 0.851). N , sEPSC decay tau in WT neurons comparing hungry vs fed states (NS, p = 0.142). O , Representative sEPSC traces in OGT-KO neurons under hungry ( top ) and fed ( bottom ) conditions. P , sEPSC frequency in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.095). Q , sEPSC amplitude in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.309). R , sEPSC decay tau in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.547). All sEPSC data ( panels K–R ) were obtained from WT (n = 5) and OGT-KO (n = 5) mice under hungry and fed conditions (n = 5 each condition). Statistical comparisons using Mann-Whitney non-parametric test. Data presented as mean ± SEM. Blue bars: WT neurons ( dark blue = hungry, light blue = fed); yellow bars: OGT-KO neurons ( light yellow = hungry, bright yellow = fed). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, NS, non-significant.
    Figure Legend Snippet: Nutritional state differentially modulates synaptic transmission in WT and OGT-KO neurons. A , representative trace showing suppression of spontaneous excitatory synaptic events following NBQX application in WT PVN αCaMKII neurons. The red arrow represents a typical excitatory spike. B – D , representative Western blots and quantification of total and surface GluA1 expression in primary cortical neurons. WT values were normalized to 1. OGT-KO total GluA1 = 1.282 ± 0.068; surface GluA1 = 1.629 ± 0.064. E–G , representative Western blots and quantification of total and surface GluA2 expression. WT values were normalized to 1. OGT-KO total GluA2 = 0.7101 ± 0.081; surface GluA2 = 0.5138 ± 0.075. H–J , representative Western blots and quantification of total and surface GluA3 expression. WT values were normalized to 1. OGT-KO total GluA3 = 0.6691 ± 0.032; surface GluA3 = 0.3935 ± 0.044. Western blot quantitative analyses were performed using the Wilcoxon signed-rank test. All Western blot data expressed as mean ± SEM. K , representative sEPSC traces in WT neurons under hungry ( top ) and fed ( bottom ) conditions. L , sEPSC frequency in WT neurons comparing hungry vs fed states ( p = 0.021). M , sEPSC amplitude in WT neurons comparing hungry vs fed states (NS, p = 0.851). N , sEPSC decay tau in WT neurons comparing hungry vs fed states (NS, p = 0.142). O , Representative sEPSC traces in OGT-KO neurons under hungry ( top ) and fed ( bottom ) conditions. P , sEPSC frequency in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.095). Q , sEPSC amplitude in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.309). R , sEPSC decay tau in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.547). All sEPSC data ( panels K–R ) were obtained from WT (n = 5) and OGT-KO (n = 5) mice under hungry and fed conditions (n = 5 each condition). Statistical comparisons using Mann-Whitney non-parametric test. Data presented as mean ± SEM. Blue bars: WT neurons ( dark blue = hungry, light blue = fed); yellow bars: OGT-KO neurons ( light yellow = hungry, bright yellow = fed). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, NS, non-significant.

    Techniques Used: Transmission Assay, Western Blot, Expressing, MANN-WHITNEY



    Similar Products

    glua3  (Alomone Labs)
    94
    Alomone Labs glua3
    Nutritional state differentially modulates synaptic transmission in WT and OGT-KO neurons. A , representative trace showing suppression of spontaneous excitatory synaptic events following NBQX application in WT PVN αCaMKII neurons. The red arrow represents a typical excitatory spike. B – D , representative Western blots and quantification of total and surface GluA1 expression in primary cortical neurons. WT values were normalized to 1. OGT-KO total GluA1 = 1.282 ± 0.068; surface GluA1 = 1.629 ± 0.064. E–G , representative Western blots and quantification of total and surface GluA2 expression. WT values were normalized to 1. OGT-KO total GluA2 = 0.7101 ± 0.081; surface GluA2 = 0.5138 ± 0.075. H–J , representative Western blots and quantification of total and surface <t>GluA3</t> expression. WT values were normalized to 1. OGT-KO total GluA3 = 0.6691 ± 0.032; surface GluA3 = 0.3935 ± 0.044. Western blot quantitative analyses were performed using the Wilcoxon signed-rank test. All Western blot data expressed as mean ± SEM. K , representative sEPSC traces in WT neurons under hungry ( top ) and fed ( bottom ) conditions. L , sEPSC frequency in WT neurons comparing hungry vs fed states ( p = 0.021). M , sEPSC amplitude in WT neurons comparing hungry vs fed states (NS, p = 0.851). N , sEPSC decay tau in WT neurons comparing hungry vs fed states (NS, p = 0.142). O , Representative sEPSC traces in OGT-KO neurons under hungry ( top ) and fed ( bottom ) conditions. P , sEPSC frequency in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.095). Q , sEPSC amplitude in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.309). R , sEPSC decay tau in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.547). All sEPSC data ( panels K–R ) were obtained from WT (n = 5) and OGT-KO (n = 5) mice under hungry and fed conditions (n = 5 each condition). Statistical comparisons using Mann-Whitney non-parametric test. Data presented as mean ± SEM. Blue bars: WT neurons ( dark blue = hungry, light blue = fed); yellow bars: OGT-KO neurons ( light yellow = hungry, bright yellow = fed). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, NS, non-significant.
    Glua3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agc-010-gp/pmc12870757-267-37-38?v=Alomone+Labs
    Average 94 stars, based on 1 article reviews
    glua3 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    mglur3 glutamate receptor  (Alomone Labs)
    94
    Alomone Labs mglur3 glutamate receptor
    The white dashed lines in all panels mark the cortex-striatum boundary. a Glutamate (Glu) and DAPI staining. The white arrows highlight Glu-positive signals in the striatum, presenting an increase after MPTP administration. b Glu receptor <t>(mGluR3)</t> and DAPI staining. c Glu and mGluR3 receptor staining. The yellow arrows indicate double-stained areas in the striatum, demonstrating enhanced expression in the MPTP-treated mouse. d GFAP and DAPI staining, with an increased GFAP signal in the MPTP group, reflecting higher astrocytic activation (with DAPI providing background cell body visualization). e A merged image combining the signals from ( a , b , d ). f Coomassie blue staining. The increased staining intensity in the MPTP-treated brain suggests an increase in total protein content.
    Mglur3 Glutamate Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agc-010-gp/pmc12722214-267-10-14?v=Alomone+Labs
    Average 94 stars, based on 1 article reviews
    mglur3 glutamate receptor - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Nutritional state differentially modulates synaptic transmission in WT and OGT-KO neurons. A , representative trace showing suppression of spontaneous excitatory synaptic events following NBQX application in WT PVN αCaMKII neurons. The red arrow represents a typical excitatory spike. B – D , representative Western blots and quantification of total and surface GluA1 expression in primary cortical neurons. WT values were normalized to 1. OGT-KO total GluA1 = 1.282 ± 0.068; surface GluA1 = 1.629 ± 0.064. E–G , representative Western blots and quantification of total and surface GluA2 expression. WT values were normalized to 1. OGT-KO total GluA2 = 0.7101 ± 0.081; surface GluA2 = 0.5138 ± 0.075. H–J , representative Western blots and quantification of total and surface GluA3 expression. WT values were normalized to 1. OGT-KO total GluA3 = 0.6691 ± 0.032; surface GluA3 = 0.3935 ± 0.044. Western blot quantitative analyses were performed using the Wilcoxon signed-rank test. All Western blot data expressed as mean ± SEM. K , representative sEPSC traces in WT neurons under hungry ( top ) and fed ( bottom ) conditions. L , sEPSC frequency in WT neurons comparing hungry vs fed states ( p = 0.021). M , sEPSC amplitude in WT neurons comparing hungry vs fed states (NS, p = 0.851). N , sEPSC decay tau in WT neurons comparing hungry vs fed states (NS, p = 0.142). O , Representative sEPSC traces in OGT-KO neurons under hungry ( top ) and fed ( bottom ) conditions. P , sEPSC frequency in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.095). Q , sEPSC amplitude in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.309). R , sEPSC decay tau in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.547). All sEPSC data ( panels K–R ) were obtained from WT (n = 5) and OGT-KO (n = 5) mice under hungry and fed conditions (n = 5 each condition). Statistical comparisons using Mann-Whitney non-parametric test. Data presented as mean ± SEM. Blue bars: WT neurons ( dark blue = hungry, light blue = fed); yellow bars: OGT-KO neurons ( light yellow = hungry, bright yellow = fed). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, NS, non-significant.

    Journal: The Journal of Biological Chemistry

    Article Title: O-GlcNAc transferase couples nutrient availability to synaptic plasticity in paraventricular neurons to regulate satiety

    doi: 10.1016/j.jbc.2025.111124

    Figure Lengend Snippet: Nutritional state differentially modulates synaptic transmission in WT and OGT-KO neurons. A , representative trace showing suppression of spontaneous excitatory synaptic events following NBQX application in WT PVN αCaMKII neurons. The red arrow represents a typical excitatory spike. B – D , representative Western blots and quantification of total and surface GluA1 expression in primary cortical neurons. WT values were normalized to 1. OGT-KO total GluA1 = 1.282 ± 0.068; surface GluA1 = 1.629 ± 0.064. E–G , representative Western blots and quantification of total and surface GluA2 expression. WT values were normalized to 1. OGT-KO total GluA2 = 0.7101 ± 0.081; surface GluA2 = 0.5138 ± 0.075. H–J , representative Western blots and quantification of total and surface GluA3 expression. WT values were normalized to 1. OGT-KO total GluA3 = 0.6691 ± 0.032; surface GluA3 = 0.3935 ± 0.044. Western blot quantitative analyses were performed using the Wilcoxon signed-rank test. All Western blot data expressed as mean ± SEM. K , representative sEPSC traces in WT neurons under hungry ( top ) and fed ( bottom ) conditions. L , sEPSC frequency in WT neurons comparing hungry vs fed states ( p = 0.021). M , sEPSC amplitude in WT neurons comparing hungry vs fed states (NS, p = 0.851). N , sEPSC decay tau in WT neurons comparing hungry vs fed states (NS, p = 0.142). O , Representative sEPSC traces in OGT-KO neurons under hungry ( top ) and fed ( bottom ) conditions. P , sEPSC frequency in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.095). Q , sEPSC amplitude in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.309). R , sEPSC decay tau in OGT-KO neurons comparing hungry vs fed states (NS, p = 0.547). All sEPSC data ( panels K–R ) were obtained from WT (n = 5) and OGT-KO (n = 5) mice under hungry and fed conditions (n = 5 each condition). Statistical comparisons using Mann-Whitney non-parametric test. Data presented as mean ± SEM. Blue bars: WT neurons ( dark blue = hungry, light blue = fed); yellow bars: OGT-KO neurons ( light yellow = hungry, bright yellow = fed). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, NS, non-significant.

    Article Snippet: Membranes were blocked in 5% non-fat milk prepared in TBS-T and incubated overnight at 4 °C with primary antibodies against OGT (Proteintech, 11576-2-AP; 1:2000), HSP70 (Proteintech, 10995-1-AP; 1:10,000), GluA1 (NeuroMab, N355/1; 5μg/blot), GluA2 (NeuroMab, L21/32; 5μg/blot), or GluA3 (Almone labs, AGC-010-GP; 1:1000).

    Techniques: Transmission Assay, Western Blot, Expressing, MANN-WHITNEY

    The white dashed lines in all panels mark the cortex-striatum boundary. a Glutamate (Glu) and DAPI staining. The white arrows highlight Glu-positive signals in the striatum, presenting an increase after MPTP administration. b Glu receptor (mGluR3) and DAPI staining. c Glu and mGluR3 receptor staining. The yellow arrows indicate double-stained areas in the striatum, demonstrating enhanced expression in the MPTP-treated mouse. d GFAP and DAPI staining, with an increased GFAP signal in the MPTP group, reflecting higher astrocytic activation (with DAPI providing background cell body visualization). e A merged image combining the signals from ( a , b , d ). f Coomassie blue staining. The increased staining intensity in the MPTP-treated brain suggests an increase in total protein content.

    Journal: npj Imaging

    Article Title: Quantitative multi-metabolite imaging of Parkinson’s disease using AI boosted molecular MRI

    doi: 10.1038/s44303-025-00130-x

    Figure Lengend Snippet: The white dashed lines in all panels mark the cortex-striatum boundary. a Glutamate (Glu) and DAPI staining. The white arrows highlight Glu-positive signals in the striatum, presenting an increase after MPTP administration. b Glu receptor (mGluR3) and DAPI staining. c Glu and mGluR3 receptor staining. The yellow arrows indicate double-stained areas in the striatum, demonstrating enhanced expression in the MPTP-treated mouse. d GFAP and DAPI staining, with an increased GFAP signal in the MPTP group, reflecting higher astrocytic activation (with DAPI providing background cell body visualization). e A merged image combining the signals from ( a , b , d ). f Coomassie blue staining. The increased staining intensity in the MPTP-treated brain suggests an increase in total protein content.

    Article Snippet: Antibodies against glutamate (#AB5018, Sigma Aldrich), GFAP (#Ab4674, Abcam), and mGluR3 glutamate receptor (#AGC-010-GP, Alomone labs) were used for IHC tissue staining.

    Techniques: Staining, Expressing, Activation Assay