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rabbit anti gaba a α2r  (Alomone Labs)


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    Alomone Labs rabbit anti gaba a α2r
    Effect of EA on immunoactivity of SP and <t>GABA</t> <t>A</t> <t>α2R</t> in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.
    Rabbit Anti Gaba A α2r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aga-002/pmc06342219-65-5-11?v=Alomone+Labs
    Average 93 stars, based on 13 article reviews
    rabbit anti gaba a α2r - by Bioz Stars, 2026-07
    93/100 stars

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    1) Product Images from "Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats"

    Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

    Journal: Journal of Pain Research

    doi: 10.2147/JPR.S180165

    Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.
    Figure Legend Snippet: Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.

    Techniques Used: Immunofluorescence

    Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.
    Figure Legend Snippet: Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.

    Techniques Used: Expressing



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    Image Search Results


    Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.

    Journal: Journal of Pain Research

    Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

    doi: 10.2147/JPR.S180165

    Figure Lengend Snippet: Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.

    Article Snippet: The membrane was incubated with rabbit anti-GABA A α2R (1:1,000, AGA-002; Alomone Labs) and rabbit anti-GAB-A B R1 (1:1,000, ab90883; Abcam).

    Techniques: Immunofluorescence

    Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.

    Journal: Journal of Pain Research

    Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

    doi: 10.2147/JPR.S180165

    Figure Lengend Snippet: Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.

    Article Snippet: The membrane was incubated with rabbit anti-GABA A α2R (1:1,000, AGA-002; Alomone Labs) and rabbit anti-GAB-A B R1 (1:1,000, ab90883; Abcam).

    Techniques: Expressing

    Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.

    Journal: Journal of Pain Research

    Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

    doi: 10.2147/JPR.S180165

    Figure Lengend Snippet: Effect of EA on immunoactivity of SP and GABA A α2R in the cervical C 3–6 DRGs in neck incisional pain rats. Notes: ( A ) Representative images of SP (green) and GABA A α2R (red) expressions in the C3–6 DRGs. GABA A α2R was expressed on SP-positive neurons. ( B ) Average immunofluorescence intensity of SP and GABA A α2R in C3–6 DRGs. The immunoactivity of SP and GABA A α2R was increased and decreased, respectively, after incisional operation compared with normal rats, and after EA at LI18 and LI4–PC6, the immunoactivity of SP was downregulated and of GABA A α2R upregulated compared with the model group (n=5 per group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. ΔΔΔ P <0.001 vs the normal group, *** P <0.001 vs the model group, ▲▲▲ P <0.001 vs the LI18 group, # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid; SP, substance P.

    Article Snippet: Sections were blocked with 5% donkey serum for 30 minutes and incubated with GABA A α2R (AGA-002; Alomone Labs, Jerusalem, Israel) and SP (sc58591; Santa Cruz Biotechnology Inc., Dallas, TX, USA), or GABA B R1 (ab90883; Abcam, Cambridge, MA, USA) and GFAP (a marker for SGCs activities, 3670S; Cell Signaling) at 4°C overnight, washed three times with 0.1 M PBS, and then incubated in donkey anti-mouse IgG conjugated Alexa Fluor488 (Thermo Fisher Scientific) and donkey anti-rabbit IgG conjugated Alexa Fluor594 (Thermo Fisher Scientific) for 2 hours under room temperature.

    Techniques: Immunofluorescence

    Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.

    Journal: Journal of Pain Research

    Article Title: Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats

    doi: 10.2147/JPR.S180165

    Figure Lengend Snippet: Effect of EA on expression of GABA A α2R mRNA and protein in C3–6 DRGs of incisional neck pain rats. Notes: ( A ) The mRNA expression of GABA A α2R and ( B ) the protein expression of GABA A α2R. Expression of GABA A α2R mRNA and protein was decreased in the model group in comparison with the normal group. EA at LI18 and LI4–PC6 could upregulate the expression of GABA A α2R mRNA and protein in C3–6 DRGs compared with the model group (n=5 in each group). Data were analyzed using one-way ANOVA and expressed as mean±SD of the mean. Δ P <0.05, ΔΔΔ P <0.001 vs the normal group; * P <0.05, *** P <0.001 vs the model group; ▲ P <0.05 vs the LI18 group; # P <0.05 vs the LI4–PC6 group. Abbreviations: DRG, dorsal root ganglion; EA, electroacupuncture; GABA, γ-aminobutyric acid.

    Article Snippet: Sections were blocked with 5% donkey serum for 30 minutes and incubated with GABA A α2R (AGA-002; Alomone Labs, Jerusalem, Israel) and SP (sc58591; Santa Cruz Biotechnology Inc., Dallas, TX, USA), or GABA B R1 (ab90883; Abcam, Cambridge, MA, USA) and GFAP (a marker for SGCs activities, 3670S; Cell Signaling) at 4°C overnight, washed three times with 0.1 M PBS, and then incubated in donkey anti-mouse IgG conjugated Alexa Fluor488 (Thermo Fisher Scientific) and donkey anti-rabbit IgG conjugated Alexa Fluor594 (Thermo Fisher Scientific) for 2 hours under room temperature.

    Techniques: Expressing