Journal: The Journal of Headache and Pain
Article Title: ROS/TRPA1/CGRP signaling mediates cortical spreading depression
doi: 10.1186/s10194-019-0978-z
Figure Lengend Snippet: Representative images showing immunohistochemistry detection of TRPA1 expression in neurons and astrocytes of rat cerebral cortex. Double immune-labelling showed TRPA1 expressed in cerebral cortical neurons (upper panel, white arrows) and astrocytes (lower panel, white arrows). DAPI staining indicates nucleus is shown in blue; TRPA1 in red, NeuN indicates neurons or GFAP indicates glial cells shown in green
Article Snippet: Double staining was performed with anti-TRPA1 antibody (1:200, Alomone Labs, ACC-0037) and either anti-NeuN antibody (1:1000, MAB377) indicating neurons or anti-glial fibrillary acidic protein (GFAP) antibody (1:200, CST3670) indicating glial cells.
Techniques: Immunohistochemistry, Expressing, Staining