adv5 peptivator  (Miltenyi Biotec)

Journal: Journal of Medical Virology

Article Title: Adenovirus‐Specific T Cells in Adults Are Frequent, Cross‐Reactive to Common Childhood Adenovirus Infections and Boosted by Adenovirus‐Vectored Vaccines

doi: 10.1002/jmv.70222

Figure Lengend Snippet: Proteins and peptide pools used for T cell stimulation.

Article Snippet: PepTivator AdV5 Hexon (130‐093‐495) , Miltenyi , C: 5 , 2 μg/mL/peptide.

Techniques: Concentration Assay

Journal: Journal of Medical Virology

Article Title: Adenovirus‐Specific T Cells in Adults Are Frequent, Cross‐Reactive to Common Childhood Adenovirus Infections and Boosted by Adenovirus‐Vectored Vaccines

doi: 10.1002/jmv.70222

Figure Lengend Snippet: Analysis of AdV specific IFNγ and IL2 FluoroSpot responses to AdV peptide pools (A) IFNγ FluoroSpot responses to AdV peptide pools covering: an experimentally‐validated set of 50 AdV C2 and 5 peptides (AdSelect), peptide pools covering the entire hexons of human adenoviruses A12, B3, C5, D26, F41 and the hexon of chimpanzee adenovirus Y25 (vector backbone of SARS‐CoV‐2 vaccine ChAdOx1), as well as a polyclonal anti‐CD3/CD28 antibody T cell stimulation as a positive control of PBMC from healthy blood donors, calculated as spot‐forming units (SFU) per 10 6 PBMC (background corrected). (B) IL2 FluoroSpot responses to AdV peptide pools covering: an experimentally‐validated set of 50 AdV C2 and 5 peptides (AdSelect), pools covering the entire hexons of human adenoviruses A12, B3, C5, D26, F41, the hexon of chimpanzee adenovirus Y25 (vector backbone of SARS‐CoV‐2 vaccine ChAdOx1), as well as a polyclonal anti‐CD3/CD28 antibody T cell stimulation as a positive control of PBMC from healthy blood donors, calculated as spot‐forming units (SFU) per 10 6 PBMC (background corrected). Significance determined by two‐way ANOVA, corrected for multiple testing. Key: * p < 0.05. The dotted lines indicate the boundary between a positive and a negative response.

Article Snippet: PepTivator AdV5 Hexon (130‐093‐495) , Miltenyi , C: 5 , 2 μg/mL/peptide.

Techniques: Plasmid Preparation, Cell Stimulation, Positive Control

Journal: Journal of Medical Virology

Article Title: Adenovirus‐Specific T Cells in Adults Are Frequent, Cross‐Reactive to Common Childhood Adenovirus Infections and Boosted by Adenovirus‐Vectored Vaccines

doi: 10.1002/jmv.70222

Figure Lengend Snippet: Frequency of IFNγ (A) and IL2 (B) responses to adenovirus peptide pools covering the hexon proteins of Y25 and C5 in ChAdOx1 vaccine recipients and controls (mRNA vaccine or no vaccine), expressed as spot‐forming units per 10 6 PBMC (background corrected). IFNγ responses are shown as filled bars. IL‐2 responses are shown as bars with horizontal stripes. Mean individual responses to ChAdV‐Y25 are shown as triangles. Mean individual responses to HAdV‐C5 are shown as circles. Control recipients are shown in purple, ChAdOx1 recipients in blue. P values were calculated with a Mann–Whitney U test.

Article Snippet: PepTivator AdV5 Hexon (130‐093‐495) , Miltenyi , C: 5 , 2 μg/mL/peptide.

Techniques: Control, MANN-WHITNEY

Journal: Journal of Medical Virology

Article Title: Adenovirus‐Specific T Cells in Adults Are Frequent, Cross‐Reactive to Common Childhood Adenovirus Infections and Boosted by Adenovirus‐Vectored Vaccines

doi: 10.1002/jmv.70222

Figure Lengend Snippet: Plots showing the correlation between the frequency of IFNγ responses for pairs of hexons for ten healthy donors. Each axis shows the number of SFUs per 10 6 PBMC produced by each donor in response to hexon peptide pool stimulation. Spearman's r correlation coefficients and two‐tailed p values are shown for statistically significant correlations.

Article Snippet: PepTivator AdV5 Hexon (130‐093‐495) , Miltenyi , C: 5 , 2 μg/mL/peptide.

Techniques: Produced, Two Tailed Test

Journal: Journal of Medical Virology

Article Title: Adenovirus‐Specific T Cells in Adults Are Frequent, Cross‐Reactive to Common Childhood Adenovirus Infections and Boosted by Adenovirus‐Vectored Vaccines

doi: 10.1002/jmv.70222

Figure Lengend Snippet: (A) Diagram showing the location of the adenovirus A12 hexon in genomic context, and the composition of the conserved and variable peptide pools derived from this protein. (B) Frequency of PBMC IFNγ responses to the HAdV‐A12 hexon, divided into two conserved and one variable epitope pool. Points show the mean ( n = 3) and SEM for each donor and pool. The dotted line indicates a positive IFNγ response was defined as greater than 32.5 SFU/10 6 PBMC (background corrected). (C) Frequency of PBMC IL2 responses to the conserved and variable epitope pools. The dotted line indicates a positive IL2 response was defined as greater than 17 SFU/10 6 PBMC (background corrected).

Article Snippet: PepTivator AdV5 Hexon (130‐093‐495) , Miltenyi , C: 5 , 2 μg/mL/peptide.

Techniques: Derivative Assay

Journal: Journal of Medical Virology

Article Title: Adenovirus‐Specific T Cells in Adults Are Frequent, Cross‐Reactive to Common Childhood Adenovirus Infections and Boosted by Adenovirus‐Vectored Vaccines

doi: 10.1002/jmv.70222

Figure Lengend Snippet: Proteins and peptide pools used for T cell stimulation.

Article Snippet: PepTivator AdV5 Penton (130‐096‐777) , Miltenyi , C: 5 , 2 μg/mL/peptide.

Techniques: Concentration Assay

Journal: Journal of Medical Virology

Article Title: Adenovirus‐Specific T Cells in Adults Are Frequent, Cross‐Reactive to Common Childhood Adenovirus Infections and Boosted by Adenovirus‐Vectored Vaccines

doi: 10.1002/jmv.70222

Figure Lengend Snippet: Analysis of AdV specific IFNγ and IL2 FluoroSpot responses to AdV peptide pools (A) IFNγ FluoroSpot responses to AdV peptide pools covering: the pentons of human adenoviruses C5 and D26, and the penton of chimpanzee adenovirus Y25 (vector backbone ChAdOx1) as well as a polyclonal anti‐CD3/CD28 antibody T cell stimulation as a positive control of PBMC from healthy blood donors, calculated as spot‐forming units (SFU) per 10 6 PBMC (background corrected). (B) IL2 FluoroSpot responses to AdV peptide pools covering: the pentons of human adenoviruses C5 and D26, and the penton of chimpanzee adenovirus Y25 (vector backbone ChAdOx1) as well as a polyclonal anti‐CD3/CD28 antibody T cell stimulation as a positive control of PBMC from healthy blood donors, calculated as spot‐forming units (SFU) per 10 6 PBMC (background corrected).

Article Snippet: PepTivator AdV5 Penton (130‐096‐777) , Miltenyi , C: 5 , 2 μg/mL/peptide.

Techniques: Plasmid Preparation, Cell Stimulation, Positive Control

Journal: Vaccines

Article Title: Effect of Prior ChAdOx1 COVID-19 Immunisation on T-Cell Responses to ChAdOx1-HBV †

doi: 10.3390/vaccines12060644

Figure Lengend Snippet: Gamma-interferon ELISpot responses to the AdV5 Hexon peptide pool. ( A , B ): Line graphs represent AdV5 Hexon responses (SFU/10 6 PBMC) for each participant across the study for PC and PM cohorts, respectively. Red arrow indicates ChAdOx1-HBV administration. ( C , D ): Correlation of baseline AdV5 Hexon responses to the sum of HBV-specific peptide response (Core, Pol1+2, Pol3+4, and PreS1/S2+S) on day 14 and day 28, respectively (SFU/10 6 PBMC). Correlations were assessed using Spearman tests.

Article Snippet: The plates were washed and blocked with Complete Media for 2 h. The plates were then loaded with 2 μg/peptide/mL of 15-mer peptides (overlapping by 11 amino acids) covering the HBV core, polymerase (Pol), and surface HBV antigen sequences (Mimotopes), arranged into four pools (Core, Polymerase 1+2, Polymerase 3+4, Pre-S1+S2 and Surface) or AdV5 Hexon (PepTivator ® , Miltenyi Biotec, Bisley, UK), representing a structural protein in the ChAdOx viral coat and an immunodominant T-cell target among capsid proteins, containing multiple epitopes conserved among serotypes.

Techniques: Enzyme-linked Immunospot