active center specific monoclonal antibody  (TaKaRa)

 
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    Structured Review

    TaKaRa active center specific monoclonal antibody
    Active Center Specific Monoclonal Antibody, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/active center specific monoclonal antibody/product/TaKaRa
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    active center specific monoclonal antibody - by Bioz Stars, 2019-10
    91/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Real-time Polymerase Chain Reaction:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Real-time PCR was performed (CFX96 thermal cycler, Bio-Rad Laboratories, USA) and tests were manually set-up. .. Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Article Title: miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
    Article Snippet: Paragraph title: Real-time PCR ... Total volume of each reaction was 30 μL and encompassed 3 μL of cDNA, 1x PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP (Biosan, Russia), 1 % BSA, 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Article Title: miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
    Article Snippet: Reaction mix contained 3 μL of RNA preparation, 16.7 % trehalose, 1x RT-PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP, 1 % BSA, 100U M-MLV reverse transcriptase (Vector-Best, Russia), 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 μM of each primer and 0.25 μM of dual-labeled probe. .. Reaction mix contained 3 μL of RNA preparation, 16.7 % trehalose, 1x RT-PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP, 1 % BSA, 100U M-MLV reverse transcriptase (Vector-Best, Russia), 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Amplification:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Article Title: miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
    Article Snippet: Total volume of each reaction was 30 μL and encompassed 3 μL of cDNA, 1x PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP (Biosan, Russia), 1 % BSA, 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best), 0.5 μM of each primer and 0.25 μM of dual-labeled probe. .. U6 snRNA served as an internal normalization control because it is most commonly used for this purpose in studies on the expression of miRNA [ ].

    Polymerase Chain Reaction:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Real-time PCR was performed (CFX96 thermal cycler, Bio-Rad Laboratories, USA) and tests were manually set-up. .. Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe. .. PCR cycling conditions were: 2 min incubation at 50 °C; 2 min pre-denaturation step at 94 °C; 50 denaturation cycles (94 °C for 10 s) alternated with annealing and elongation (60 °С for 20 s).

    Article Title: miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
    Article Snippet: Total volume of each reaction was 30 μL and encompassed 3 μL of cDNA, 1x PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP (Biosan, Russia), 1 % BSA, 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best), 0.5 μM of each primer and 0.25 μM of dual-labeled probe. .. U6 snRNA served as an internal normalization control because it is most commonly used for this purpose in studies on the expression of miRNA [ ].

    Produced:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Oligonucleotide sequences were designed (PrimerQuest online software, Integrated DNA Technologies, USA) and produced (Vector-Best, Novosibirsk, Russia). .. Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
    Article Snippet: Detection was performed using Real-time PCR combined with reverse transcription reaction in a single tube. .. Reaction mix contained 3 μL of RNA preparation, 16.7 % trehalose, 1x RT-PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP, 1 % BSA, 100U M-MLV reverse transcriptase (Vector-Best, Russia), 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 μM of each primer and 0.25 μM of dual-labeled probe. .. RT-PCR protocol: incubation at 45 °С - 30 min., heating at 95 °С - 2 min., 50 cycles of denaturation at 94 °С - 10 s, annealing and elongation: 60 °С - 20 s. Sequences of primers and the probe are listed in Additional file .

    Expressing:

    Article Title: miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
    Article Snippet: Total volume of each reaction was 30 μL and encompassed 3 μL of cDNA, 1x PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP (Biosan, Russia), 1 % BSA, 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best), 0.5 μM of each primer and 0.25 μM of dual-labeled probe. .. PCR cycling conditions were as follows: 2 min incubation at 50 °C, pre-denaturation step at 94 °C – 2 min, followed by 50 cycles of denaturation (94 °C for 10 s), annealing and elongation (60 °С for 20 s).

    Sequencing:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Translocation Assay:

    Article Title: miRNA profiling, detection of BRAF V600E mutation and RET-PTC1 translocation in patients from Novosibirsk oblast (Russia) with different types of thyroid tumors
    Article Snippet: Paragraph title: Detection of RET-PTC1 translocation ... Reaction mix contained 3 μL of RNA preparation, 16.7 % trehalose, 1x RT-PCR buffer (Vector-Best, Russia), 0.4 mM of each dNTP, 1 % BSA, 100U M-MLV reverse transcriptase (Vector-Best, Russia), 1U Taq polymerase (Vector-Best, Russia) pre-mixed with active center-specific monoclonal antibody (Clontech, USA), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Plasmid Preparation:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe. .. Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

    Software:

    Article Title: Dog survey in Russian veterinary hospitals: tick identification and molecular detection of tick-borne pathogens
    Article Snippet: Oligonucleotide sequences were designed (PrimerQuest online software, Integrated DNA Technologies, USA) and produced (Vector-Best, Novosibirsk, Russia). .. Each reaction required 50 μl total volume and included 1× PCR buffer (Vector-Best, Novosibirsk, Russia), 0.4 mM of dNTP (Biosan, Russia), 1% BSA and 1U Taq polymerase (Vector-Best, Novosibirsk, Russia) pre-mixed with active center-specific monoclonal antibody (Takara Bio, Mountain View, CA, USA), 0.5 units of uracil-DNA glycosylase (Vector-Best, Novosibirsk, Russia), 0.5 μM of each primer and 0.25 μM of dual-labeled probe.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    TaKaRa active center specific monoclonal antibody
    Active Center Specific Monoclonal Antibody, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/active center specific monoclonal antibody/product/TaKaRa
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    active center specific monoclonal antibody - by Bioz Stars, 2019-10
    91/100 stars
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