Structured Review

TaKaRa center specific monoclonal antibody
Center Specific Monoclonal Antibody, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/center specific monoclonal antibody/product/TaKaRa
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
center specific monoclonal antibody - by Bioz Stars, 2020-02
90/100 stars

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Related Articles

Synthesized:

Article Title: A Barley Efflux Transporter Operates in a Na+-Dependent Manner, as Revealed by a Multidisciplinary Platform [OPEN]
Article Snippet: Aliquots of nondigested and TEV protease-treated liposomes were detected by immunoblot analyses using a mouse IgG2a isotype anti 6xHis monoclonal antibody (Clontech) and a crude serum raised against the Bot1 peptide specified above. .. The crude serum with Bot1 antibodies was prepared by immunization in rabbits (Institute for Medical and Veterinary Medicine) using a peptide synthesized by Mimotopes.

Article Title: Coactivator functions in a stoichiometric complex with anaphase-promoting complex/cyclosome to mediate substrate recognition
Article Snippet: Substrates and coactivators were synthesized using the TNT T7 Quick coupled in vitro transcription/translation kit (Promega, Madison, WI, USA) at 30°C for 90 min. Redivue L -[35 S]methionine (Amersham, Little Chalfont, Buckinghamshire, UK) was used for labelling proteins. .. Next, 2 μl of 6His monoclonal antibody (Clontech, Mountain View, CA, USA) or 2 μl of antibody buffer (50% glycerol) was added for a total volume of 14 μl.

Construct:

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain. .. Secondary antibodies: rabbit anti-goat IgG-HRP (Jackson ImmunoResearch; 1:2500), goat anti-rabbit IgG-HRP (Jackson ImmunoResearch; 1:2500), goat anti-mouse IgG-HRP (Jackson ImmunoResearch; 1:2500).

Electrophoresis:

Article Title: Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
Article Snippet: After electrophoresis and blotting GFP was detected by a 1∶4,000 dilution of the Living Colors A.v. .. Monoclonal Antibody (Clontech Laboratories) and a 1∶1,000 dilution of an anti-mouse-HRP conjugate (dianova, Hamburg, Germany). β-Actin was detected by a 1∶20,000 dilution by a directly HRP linked antibody (dianova).

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: After the electrophoresis the proteins were transferred to PVDF membrane (Thermo) for 30 min at 1 Amp and 25 V. Then the membrane was blocked in TBS containing 0.1% Tween 20 and 1% BSA at 4°C overnight. .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain.

Incubation:

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: Reaction mixtures were incubated at 37°C for 15 to 60 min, and the reactions were stopped by the addition of 2× sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer. .. Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

Article Title: Antigenic cartography of H1N1 influenza viruses using sequence-based antigenic distance calculation
Article Snippet: Plates were washed and incubated with alkaline phosphatase (AP)-conjugated secondary antibody (Southern Biotech 1030–04 or LSBio LS- ) for 2 h at room temperature. .. Mouse monoclonal antibody titers for each HA were derived from a standard curve created using C179 universally HA binding monoclonal antibody (Takara).

Article Title: Coactivator functions in a stoichiometric complex with anaphase-promoting complex/cyclosome to mediate substrate recognition
Article Snippet: Next, 2 μl of 6His monoclonal antibody (Clontech, Mountain View, CA, USA) or 2 μl of antibody buffer (50% glycerol) was added for a total volume of 14 μl. .. The reactions were incubated at room temperature for 15 min and then 1.5 μl of native gel sample buffer (125 mM Tris pH 8.8, 84% glycerol and bromophenol blue) was added.

Article Title: Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis [OPEN]
Article Snippet: For coimmunoprecipitation assays, total protein extracts were prepared in lysis buffer (20 m m Tris-HCl, pH 7.5, 150 m m NaCl, 1 m m Ethylenediaminetetraacetic acid (EDTA), 0.5% Triton X-100, 20 µ m MG132, 1 m m phenylmethylsulfonyl fluoride, and 1% protease inhibitor cocktail [P9599 Sigma]), and incubated with anti-FLAG M2 affinity gel (A2220 Sigma) for 3 h. Immunoprecipitates were washed three times with the lysis buffer and eluted with 2× Laemmli sample buffer followed by immunoblotting. .. Monoclonal Antibody (Clontech) was used in the immunoblotting assays.

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: After blocking the membrane was incubated for 2 h at RT with primary IgG, washed and then incubated for 1 h at RT with HRP-conjugated secondary IgG. .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain.

Activity Assay:

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: The presence of PARP-cleaving activity in the extracts was determined as described elsewhere ( ); 25 to 50 μg of extract was brought up to 10 to 15 μl with EPB and mixed with 106 purified HeLa cell nuclei that had been washed and resuspended in 10 to 15 μl of mitotic dilution buffer (10 mM HEPES [pH 7.0], 40 mM α-glycerophosphate, 50 mM NaCl, 2 mM MgCl2 , 5 mM EGTA, 1 mM DTT, 2 mM ATP, 10 mM creatine phosphate, 50 μg of creatine kinase per ml). .. Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

Article Title: Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3? End of the Viral RNA
Article Snippet: It is possible that the downstream sequences disrupted a proper folding essential for the binding activity of RBD2. .. Equal amounts of the recombinant proteins as determined by Bio-Rad protein assay were subjected to Western blot analysis with 6× His monoclonal antibody (ClonTech) (B) and Northwestern analysis as previously described (

Expressing:

Article Title: A Barley Efflux Transporter Operates in a Na+-Dependent Manner, as Revealed by a Multidisciplinary Platform [OPEN]
Article Snippet: For analyses of protein synthesis and expression ( n = 2), and all transport data ( n = 3), we calculated the values of se of the mean. .. Aliquots of nondigested and TEV protease-treated liposomes were detected by immunoblot analyses using a mouse IgG2a isotype anti 6xHis monoclonal antibody (Clontech) and a crude serum raised against the Bot1 peptide specified above.

Bradford Assay:

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: In each experiment, protein in the extracts was quantitated by the Bradford assay, and equivalent concentrations of protein were added to each reaction. .. Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

Western Blot:

Article Title: DNA damage in germ cells induces immune response triggering systemic stress resistance
Article Snippet: Paragraph title: Western blot ... Monoclonal Antibody (JL-8), Clontech art-no 632381; Mouse Monoclonal anti-alfaTubulin (Clone DM 1A), Sigma-Aldrich art-no T6199; Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody 9101, Cell Signalling, NEB art-no 9101S.

Article Title: A Barley Efflux Transporter Operates in a Na+-Dependent Manner, as Revealed by a Multidisciplinary Platform [OPEN]
Article Snippet: Aliquots of nondigested and TEV protease-treated liposomes were detected by immunoblot analyses using a mouse IgG2a isotype anti 6xHis monoclonal antibody (Clontech) and a crude serum raised against the Bot1 peptide specified above. .. The immunoblots were developed with the Novex ECL HRP chemiluminescent substrate reagent kit (Invitrogen) as described above and scanned.

Article Title: Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3? End of the Viral RNA
Article Snippet: .. Equal amounts of the recombinant proteins as determined by Bio-Rad protein assay were subjected to Western blot analysis with 6× His monoclonal antibody (ClonTech) (B) and Northwestern analysis as previously described ( .. This study investigated the RNA-binding specificity of the HCV NS5B RdRp.

Article Title: Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
Article Snippet: Paragraph title: Western blot analysis ... Monoclonal Antibody (Clontech Laboratories) and a 1∶1,000 dilution of an anti-mouse-HRP conjugate (dianova, Hamburg, Germany). β-Actin was detected by a 1∶20,000 dilution by a directly HRP linked antibody (dianova).

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: Paragraph title: Western Blot ... The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain.

Article Title: Acetylome Profiling Reveals Overlap in the Regulation of Diverse Processes by Sirtuins, Gcn5, and Esa1 *
Article Snippet: Beads were washed with lysis buffer 3×, followed by elution with 40–60 μl of SDS-PAGE sample buffer with 0.1 m DTT at 65 °C for 10 min. Supernatant was boiled prior to sample separation on a 4–20% gradient gel (BioRad) and Western blotting. .. GFP was detected using a monoclonal antibody (JL-8, Clontech) at 1:1000 dilution.

Derivative Assay:

Article Title: Antigenic cartography of H1N1 influenza viruses using sequence-based antigenic distance calculation
Article Snippet: .. Mouse monoclonal antibody titers for each HA were derived from a standard curve created using C179 universally HA binding monoclonal antibody (Takara). ..

Transfection:

Article Title: Interaction of the Hepatitis B Spliced Protein with Cathepsin B Promotes Hepatoma Cell Migration and Invasion
Article Snippet: For the in vivo coimmunoprecipitation (Co-IP) experiments, transiently transfected Huh-7 cells were lysed and the soluble proteins were precleared with 100 μl of a 50% slurry of protein A agarose (Invitrogen). .. The immunoprecipitated complexes were analyzed by 10% SDS-PAGE and immunoblotting using anti-GAL4 DNA-binding domain (GAL4 BD) monoclonal antibody (Clontech) and anti-CTSB antibody (Santa Cruz Biotechnology).

Article Title: Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
Article Snippet: Western blot analysis 48 h after GNOME laser transfection with anti-GFP siRNA all samples and controls were lysed in 40 µl lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris, pH 8.0, Roche cOmplete ultra protease inhibitor) per well. .. Monoclonal Antibody (Clontech Laboratories) and a 1∶1,000 dilution of an anti-mouse-HRP conjugate (dianova, Hamburg, Germany). β-Actin was detected by a 1∶20,000 dilution by a directly HRP linked antibody (dianova).

Protease Inhibitor:

Article Title: Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis [OPEN]
Article Snippet: For coimmunoprecipitation assays, total protein extracts were prepared in lysis buffer (20 m m Tris-HCl, pH 7.5, 150 m m NaCl, 1 m m Ethylenediaminetetraacetic acid (EDTA), 0.5% Triton X-100, 20 µ m MG132, 1 m m phenylmethylsulfonyl fluoride, and 1% protease inhibitor cocktail [P9599 Sigma]), and incubated with anti-FLAG M2 affinity gel (A2220 Sigma) for 3 h. Immunoprecipitates were washed three times with the lysis buffer and eluted with 2× Laemmli sample buffer followed by immunoblotting. .. Monoclonal Antibody (Clontech) was used in the immunoblotting assays.

Article Title: Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
Article Snippet: Western blot analysis 48 h after GNOME laser transfection with anti-GFP siRNA all samples and controls were lysed in 40 µl lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris, pH 8.0, Roche cOmplete ultra protease inhibitor) per well. .. Monoclonal Antibody (Clontech Laboratories) and a 1∶1,000 dilution of an anti-mouse-HRP conjugate (dianova, Hamburg, Germany). β-Actin was detected by a 1∶20,000 dilution by a directly HRP linked antibody (dianova).

other:

Article Title: Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis
Article Snippet: The antibodies and antibiotic product codes used are as follows: anti-CLPB (Aviva, catalog# ARP53790_P050) 0.5 µg/mL, anti-DnaK (Aviva, catalog# OAED00201) 1 µg/mL, anti-TF (Clontech, catalog# M201) 2 µg/mL, anti-groEL (Abcam, catalog# ab82592) 1 µg/mL, and anti-DnaJ (Enzo Life Sciences, catalog# ADI-SPA-410-D) 0.5 µg/mL.

Article Title: Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis
Article Snippet: Antibody and antibiotic product codes The antibodies and antibiotic product codes used are as follows: anti-CLPB (Aviva, catalog# ARP53790_P050) 0.5 µg/mL, anti-DnaK (Aviva, catalog# OAED00201) 1 µg/mL, anti-TF (Clontech, catalog# M201) 2 µg/mL, anti-groEL (Abcam, catalog# ab82592) 1 µg/mL, and anti-DnaJ (Enzo Life Sciences, catalog# ADI-SPA-410-D) 0.5 µg/mL.

Sonication:

Article Title: Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
Article Snippet: Lysates were prepared by sonication, and recombinant proteins were purified from soluble extract with Ni2 + -NTA-agarose resin (Qiagen) ( ). .. Proteins were monitored by SDS-PAGE and Coomassie blue staining, immunoblot analysis with anti-6×His monoclonal antibody (Clontech), and RNA 5′-triphosphatase assay ( ).

Affinity Purification:

Article Title: Molecular Basis of Calpain Cleavage and Inactivation of the Sodium-Calcium Exchanger 1 in Heart Failure *
Article Snippet: Monoclonal antibody (JL-8), 632381) was from Clontech (Mountain View, CA). .. Anti-mouse IgG HRP (NA931V), anti-rabbit IgG HRP (NA934V) affinity-purified polyclonal antibody (both from GE Healthcare), and anti-goat IgG HRP (HAF109, R & D Systems, Minneapolis, MI) were used as secondary antibody.

Recombinant:

Article Title: Antigenic cartography of H1N1 influenza viruses using sequence-based antigenic distance calculation
Article Snippet: Recombinant HA proteins were coated on MaxiSorb 96-well plates (ThermoSci; 439,454) overnight at 4 °C. .. Mouse monoclonal antibody titers for each HA were derived from a standard curve created using C179 universally HA binding monoclonal antibody (Takara).

Article Title: Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3? End of the Viral RNA
Article Snippet: .. Equal amounts of the recombinant proteins as determined by Bio-Rad protein assay were subjected to Western blot analysis with 6× His monoclonal antibody (ClonTech) (B) and Northwestern analysis as previously described ( .. This study investigated the RNA-binding specificity of the HCV NS5B RdRp.

Article Title: Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
Article Snippet: Paragraph title: Recombinant protein production and purification ... Proteins were monitored by SDS-PAGE and Coomassie blue staining, immunoblot analysis with anti-6×His monoclonal antibody (Clontech), and RNA 5′-triphosphatase assay ( ).

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain. .. Secondary antibodies: rabbit anti-goat IgG-HRP (Jackson ImmunoResearch; 1:2500), goat anti-rabbit IgG-HRP (Jackson ImmunoResearch; 1:2500), goat anti-mouse IgG-HRP (Jackson ImmunoResearch; 1:2500).

Molecular Weight:

Article Title: Acetylome Profiling Reveals Overlap in the Regulation of Diverse Processes by Sirtuins, Gcn5, and Esa1 *
Article Snippet: GFP was detected using a monoclonal antibody (JL-8, Clontech) at 1:1000 dilution. .. Molecular weight markers are estimated from the Kaleidoscope Precision Plus protein ladder from BioRad.

Nucleic Acid Electrophoresis:

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: Reaction mixtures were incubated at 37°C for 15 to 60 min, and the reactions were stopped by the addition of 2× sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer. .. Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

In Vivo:

Article Title: Interaction of the Hepatitis B Spliced Protein with Cathepsin B Promotes Hepatoma Cell Migration and Invasion
Article Snippet: For the in vivo coimmunoprecipitation (Co-IP) experiments, transiently transfected Huh-7 cells were lysed and the soluble proteins were precleared with 100 μl of a 50% slurry of protein A agarose (Invitrogen). .. The immunoprecipitated complexes were analyzed by 10% SDS-PAGE and immunoblotting using anti-GAL4 DNA-binding domain (GAL4 BD) monoclonal antibody (Clontech) and anti-CTSB antibody (Santa Cruz Biotechnology).

Nickel Column:

Article Title: Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
Article Snippet: Proteins were monitored by SDS-PAGE and Coomassie blue staining, immunoblot analysis with anti-6×His monoclonal antibody (Clontech), and RNA 5′-triphosphatase assay ( ). .. To generate nonguanylylated Ceg1, nickel column purified protein was dialyzed against buffer B [20 m m MOPS-KOH (pH 7.0), 20 m m potassium acetate, 1 m m MgCl2 , 10% glycerol, 1 m m dithiothreitol, 1 m m EDTA, 1 m m PMSF, and 1 m m sodium pyrophosphate].

Purification:

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: The presence of PARP-cleaving activity in the extracts was determined as described elsewhere ( ); 25 to 50 μg of extract was brought up to 10 to 15 μl with EPB and mixed with 106 purified HeLa cell nuclei that had been washed and resuspended in 10 to 15 μl of mitotic dilution buffer (10 mM HEPES [pH 7.0], 40 mM α-glycerophosphate, 50 mM NaCl, 2 mM MgCl2 , 5 mM EGTA, 1 mM DTT, 2 mM ATP, 10 mM creatine phosphate, 50 μg of creatine kinase per ml). .. Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

Article Title: A Barley Efflux Transporter Operates in a Na+-Dependent Manner, as Revealed by a Multidisciplinary Platform [OPEN]
Article Snippet: Purified Bot1 liposomes were dialyzed for 14 to 18 h at 4°C using 10-kD cutoff Slide-A-Lyser Mini dialysis cups (Thermo Scientific) against 10 mM histidine buffer, adjusted with Bis-Tris propane to pH 7.5, and stored on crushed ice, where they remained stable for up to 5 d. The diameters of proteoliposomes were determined with a NICOMP 380 particle sizing system operating in a vesicle mode. .. Aliquots of nondigested and TEV protease-treated liposomes were detected by immunoblot analyses using a mouse IgG2a isotype anti 6xHis monoclonal antibody (Clontech) and a crude serum raised against the Bot1 peptide specified above.

Article Title: Coactivator functions in a stoichiometric complex with anaphase-promoting complex/cyclosome to mediate substrate recognition
Article Snippet: For binding reactions , 2 μl of IVT-produced coactivator, 2 μl of IVT-produced substrate, 2 μl of purified APC/C (∼50 ng), 0.7 μl of 100 mM CaCl2 and 5.3 μl of binding buffer (10 mM Tris pH 8, 150 mM NaCl, 3 mM dithiothreitol, 1 mM magnesium acetate and 2 mM EGTA) were mixed. .. Next, 2 μl of 6His monoclonal antibody (Clontech, Mountain View, CA, USA) or 2 μl of antibody buffer (50% glycerol) was added for a total volume of 14 μl.

Article Title: Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3? End of the Viral RNA
Article Snippet: Partially purified full-length NS5A and NS5B and NS5B deletion mutants as indicated were analyzed by sodium dodecyl sulfate–15% polyacrylamide gel electrophoresis in Tricine-Tris buffer and stained with Coomassie blue (A). .. Equal amounts of the recombinant proteins as determined by Bio-Rad protein assay were subjected to Western blot analysis with 6× His monoclonal antibody (ClonTech) (B) and Northwestern analysis as previously described (

Article Title: Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
Article Snippet: Paragraph title: Recombinant protein production and purification ... Proteins were monitored by SDS-PAGE and Coomassie blue staining, immunoblot analysis with anti-6×His monoclonal antibody (Clontech), and RNA 5′-triphosphatase assay ( ).

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain. .. Secondary antibodies: rabbit anti-goat IgG-HRP (Jackson ImmunoResearch; 1:2500), goat anti-rabbit IgG-HRP (Jackson ImmunoResearch; 1:2500), goat anti-mouse IgG-HRP (Jackson ImmunoResearch; 1:2500).

Protein Extraction:

Article Title: Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis [OPEN]
Article Snippet: Paragraph title: Plant Protein Extraction, Coimmunoprecipitation Assay, and Immunoblotting ... Monoclonal Antibody (Clontech) was used in the immunoblotting assays.

Fast Protein Liquid Chromatography:

Article Title: Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
Article Snippet: Proteins were monitored by SDS-PAGE and Coomassie blue staining, immunoblot analysis with anti-6×His monoclonal antibody (Clontech), and RNA 5′-triphosphatase assay ( ). .. Deguanylylated Ceg1 was further purified by MonoS(HR5/5) FPLC-chromatography.

Polyacrylamide Gel Electrophoresis:

Article Title: Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3? End of the Viral RNA
Article Snippet: Partially purified full-length NS5A and NS5B and NS5B deletion mutants as indicated were analyzed by sodium dodecyl sulfate–15% polyacrylamide gel electrophoresis in Tricine-Tris buffer and stained with Coomassie blue (A). .. Equal amounts of the recombinant proteins as determined by Bio-Rad protein assay were subjected to Western blot analysis with 6× His monoclonal antibody (ClonTech) (B) and Northwestern analysis as previously described (

Staining:

Article Title: Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3? End of the Viral RNA
Article Snippet: Partially purified full-length NS5A and NS5B and NS5B deletion mutants as indicated were analyzed by sodium dodecyl sulfate–15% polyacrylamide gel electrophoresis in Tricine-Tris buffer and stained with Coomassie blue (A). .. Equal amounts of the recombinant proteins as determined by Bio-Rad protein assay were subjected to Western blot analysis with 6× His monoclonal antibody (ClonTech) (B) and Northwestern analysis as previously described (

Article Title: Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
Article Snippet: .. Proteins were monitored by SDS-PAGE and Coomassie blue staining, immunoblot analysis with anti-6×His monoclonal antibody (Clontech), and RNA 5′-triphosphatase assay ( ). .. Polyhistidine tagged recombinant guanylyltransferase subunit of S. cerevisiae -capping enzyme (his7 -Ceg1) and RNA triphosphatase domain of C. elegans -capping enzyme [his7 -Cel-1(1-236)] were expressed and purified as described ( ; ).

SDS Page:

Article Title: Interaction of the Hepatitis B Spliced Protein with Cathepsin B Promotes Hepatoma Cell Migration and Invasion
Article Snippet: .. The immunoprecipitated complexes were analyzed by 10% SDS-PAGE and immunoblotting using anti-GAL4 DNA-binding domain (GAL4 BD) monoclonal antibody (Clontech) and anti-CTSB antibody (Santa Cruz Biotechnology). .. A mammalian two-hybrid assay was performed using the CheckMate mammalian two-hybrid system (Promega) according to the manufacturer's protocol.

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: Reaction mixtures were incubated at 37°C for 15 to 60 min, and the reactions were stopped by the addition of 2× sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer. .. Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

Article Title: Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
Article Snippet: .. Proteins were monitored by SDS-PAGE and Coomassie blue staining, immunoblot analysis with anti-6×His monoclonal antibody (Clontech), and RNA 5′-triphosphatase assay ( ). .. Polyhistidine tagged recombinant guanylyltransferase subunit of S. cerevisiae -capping enzyme (his7 -Ceg1) and RNA triphosphatase domain of C. elegans -capping enzyme [his7 -Cel-1(1-236)] were expressed and purified as described ( ; ).

Article Title: Acetylome Profiling Reveals Overlap in the Regulation of Diverse Processes by Sirtuins, Gcn5, and Esa1 *
Article Snippet: Beads were washed with lysis buffer 3×, followed by elution with 40–60 μl of SDS-PAGE sample buffer with 0.1 m DTT at 65 °C for 10 min. Supernatant was boiled prior to sample separation on a 4–20% gradient gel (BioRad) and Western blotting. .. GFP was detected using a monoclonal antibody (JL-8, Clontech) at 1:1000 dilution.

Software:

Article Title: A Barley Efflux Transporter Operates in a Na+-Dependent Manner, as Revealed by a Multidisciplinary Platform [OPEN]
Article Snippet: In transport data we expressed these values as a percentage of standard deviations using GraphPad Prism 6 software. .. Aliquots of nondigested and TEV protease-treated liposomes were detected by immunoblot analyses using a mouse IgG2a isotype anti 6xHis monoclonal antibody (Clontech) and a crude serum raised against the Bot1 peptide specified above.

Article Title: Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis [OPEN]
Article Snippet: Monoclonal Antibody (Clontech) was used in the immunoblotting assays. .. Blots were imaged using a Fuji LAS-4000 imager and signal quantified using TotalLab Quant software.

SDS-Gel:

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain. .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain.

Co-Immunoprecipitation Assay:

Article Title: Interaction of the Hepatitis B Spliced Protein with Cathepsin B Promotes Hepatoma Cell Migration and Invasion
Article Snippet: Paragraph title: Co-IP assay. ... The immunoprecipitated complexes were analyzed by 10% SDS-PAGE and immunoblotting using anti-GAL4 DNA-binding domain (GAL4 BD) monoclonal antibody (Clontech) and anti-CTSB antibody (Santa Cruz Biotechnology).

Article Title: Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis [OPEN]
Article Snippet: Paragraph title: Plant Protein Extraction, Coimmunoprecipitation Assay, and Immunoblotting ... Monoclonal Antibody (Clontech) was used in the immunoblotting assays.

Binding Assay:

Article Title: Antigenic cartography of H1N1 influenza viruses using sequence-based antigenic distance calculation
Article Snippet: .. Mouse monoclonal antibody titers for each HA were derived from a standard curve created using C179 universally HA binding monoclonal antibody (Takara). ..

Article Title: Coactivator functions in a stoichiometric complex with anaphase-promoting complex/cyclosome to mediate substrate recognition
Article Snippet: For binding reactions , 2 μl of IVT-produced coactivator, 2 μl of IVT-produced substrate, 2 μl of purified APC/C (∼50 ng), 0.7 μl of 100 mM CaCl2 and 5.3 μl of binding buffer (10 mM Tris pH 8, 150 mM NaCl, 3 mM dithiothreitol, 1 mM magnesium acetate and 2 mM EGTA) were mixed. .. Next, 2 μl of 6His monoclonal antibody (Clontech, Mountain View, CA, USA) or 2 μl of antibody buffer (50% glycerol) was added for a total volume of 14 μl.

Article Title: Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3? End of the Viral RNA
Article Snippet: Paragraph title: Mapping of the binding domains of NS5B to the 3′CNU RNA. ... Equal amounts of the recombinant proteins as determined by Bio-Rad protein assay were subjected to Western blot analysis with 6× His monoclonal antibody (ClonTech) (B) and Northwestern analysis as previously described (

In Vitro:

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: Paragraph title: In vitro apoptosis assay. ... Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

Article Title: Coactivator functions in a stoichiometric complex with anaphase-promoting complex/cyclosome to mediate substrate recognition
Article Snippet: Substrates and coactivators were synthesized using the TNT T7 Quick coupled in vitro transcription/translation kit (Promega, Madison, WI, USA) at 30°C for 90 min. Redivue L -[35 S]methionine (Amersham, Little Chalfont, Buckinghamshire, UK) was used for labelling proteins. .. Next, 2 μl of 6His monoclonal antibody (Clontech, Mountain View, CA, USA) or 2 μl of antibody buffer (50% glycerol) was added for a total volume of 14 μl.

Apoptosis Assay:

Article Title: Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
Article Snippet: Paragraph title: In vitro apoptosis assay. ... Proteins in the samples were resolved on SDS–10% polyacrylamide gels, transferred to nitrocellulose by the use of a semidry blotter (Fisher), and detected with a monoclonal antibody (C-2-10; Clontech) that recognizes human PARP; this was followed by addition of horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (Southern Biotechnology Associates, Inc.) and enhanced chemiluminescence (ECL) analysis (Amersham).

Immunoprecipitation:

Article Title: Interaction of the Hepatitis B Spliced Protein with Cathepsin B Promotes Hepatoma Cell Migration and Invasion
Article Snippet: .. The immunoprecipitated complexes were analyzed by 10% SDS-PAGE and immunoblotting using anti-GAL4 DNA-binding domain (GAL4 BD) monoclonal antibody (Clontech) and anti-CTSB antibody (Santa Cruz Biotechnology). .. A mammalian two-hybrid assay was performed using the CheckMate mammalian two-hybrid system (Promega) according to the manufacturer's protocol.

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain. .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain.

Article Title: Acetylome Profiling Reveals Overlap in the Regulation of Diverse Processes by Sirtuins, Gcn5, and Esa1 *
Article Snippet: Immunoprecipitation was with Ab290 (AbCam, Cambridge, MA) against GFP for 2 h, with 25 μl protein-A conjugated beads (Life Technologies, Carlsbad, CA) being added for an addition hour. .. GFP was detected using a monoclonal antibody (JL-8, Clontech) at 1:1000 dilution.

Lysis:

Article Title: Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis [OPEN]
Article Snippet: For coimmunoprecipitation assays, total protein extracts were prepared in lysis buffer (20 m m Tris-HCl, pH 7.5, 150 m m NaCl, 1 m m Ethylenediaminetetraacetic acid (EDTA), 0.5% Triton X-100, 20 µ m MG132, 1 m m phenylmethylsulfonyl fluoride, and 1% protease inhibitor cocktail [P9599 Sigma]), and incubated with anti-FLAG M2 affinity gel (A2220 Sigma) for 3 h. Immunoprecipitates were washed three times with the lysis buffer and eluted with 2× Laemmli sample buffer followed by immunoblotting. .. Monoclonal Antibody (Clontech) was used in the immunoblotting assays.

Article Title: Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
Article Snippet: Western blot analysis 48 h after GNOME laser transfection with anti-GFP siRNA all samples and controls were lysed in 40 µl lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris, pH 8.0, Roche cOmplete ultra protease inhibitor) per well. .. Monoclonal Antibody (Clontech Laboratories) and a 1∶1,000 dilution of an anti-mouse-HRP conjugate (dianova, Hamburg, Germany). β-Actin was detected by a 1∶20,000 dilution by a directly HRP linked antibody (dianova).

Article Title: Acetylome Profiling Reveals Overlap in the Regulation of Diverse Processes by Sirtuins, Gcn5, and Esa1 *
Article Snippet: Beads were washed with lysis buffer 3×, followed by elution with 40–60 μl of SDS-PAGE sample buffer with 0.1 m DTT at 65 °C for 10 min. Supernatant was boiled prior to sample separation on a 4–20% gradient gel (BioRad) and Western blotting. .. GFP was detected using a monoclonal antibody (JL-8, Clontech) at 1:1000 dilution.

Blocking Assay:

Article Title: Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin
Article Snippet: After blocking the membrane was incubated for 2 h at RT with primary IgG, washed and then incubated for 1 h at RT with HRP-conjugated secondary IgG. .. The following primary antibodies were used: anti-human paxillin (C-18) goat polyclonal antibody (Santa Cruz Biotechnology; 1:400), anti-human FAK (A-17) rabbit polyclonal antibody (Santa Cruz Biotechnology; 1:400), mouse anti 6xHis monoclonal antibody (Clontech; 1:500) for detection of MBP-LD His-tagged constructs after competition IP with recombinant purified FAT domain.

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    TaKaRa center specific monoclonal antibody
    Center Specific Monoclonal Antibody, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    center specific monoclonal antibody - by Bioz Stars, 2020-02
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