acsf buffer  (Worthington Biochemical)


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    Worthington Biochemical acsf buffer
    Acsf Buffer, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acsf buffer/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acsf buffer - by Bioz Stars, 2021-03
    86/100 stars

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    Incubation:

    Article Title: Pumilio-2 regulates translation of Nav1.6 to mediate homeostasis of membrane excitability
    Article Snippet: All experiments were conducted in accordance with the UK Animals Scientific Procedures Act (1986) and institutional regulations. .. The visual cortex was removed and placed in ice-cold ACSF buffer (126mM NaCl, 3mM KCl, 2mM MgSO4 .7H2 0, 1mM NaH2 PO4 .2H2 0, 25mM NaHCO3 , 2mM CaCl2 , and 14mM dextrose, pH 7.4), sliced into 500μm thick sections and incubated in 20 U/ml Papain (Worthington Biochemical Corp., Lakewood, NJ, USA) in Earle’s Balanced Salt Solution (1.8mM calcium EBSS supplemented with 10mM dextrose) for 90 minutes at 37°C in a 5% CO2 humidified incubator. .. After digestion, tissue was re-suspended in weak protease inhibitor (1mg/ml of both BSA and trypsin inhibitor in EBSS) and an equal amount of strong inhibitor solution (10mg/ml of both BSA and trypsin inhibitor in EBSS) was added.

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    Worthington Biochemical dnase i
    Dnase I, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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