Structured Review

Fisher Scientific acn v v
Acn V V, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acn v v/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
acn v v - by Bioz Stars, 2021-07
86/100 stars

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Related Articles

Concentration Assay:

Article Title: Rapid MALDI mass spectrometry imaging for surgical pathology
Article Snippet: Conductive indium tin oxide (ITO) coated glass slides were obtained from Bruker Daltonics (Billerica, MA). .. Generation of matrix pre-coated slides To prepare pre-coated slides, THAP was dissolved in 9:1 ACN: water (v/v) at a concentration of 40 mg/mL and sonicated for 10 min in a sonicator bath (Fisher Scientific, Pittsburgh, PA). .. The matrix solution was then applied to an ITO slide using a TM Sprayer (HTX Technologies, Chapel Hill, NC) at 30 °C, flow rate of 0.05 mL/min, velocity of 1200 mm/min and track spacing of 1.5 mm.

Sonication:

Article Title: Rapid MALDI mass spectrometry imaging for surgical pathology
Article Snippet: Conductive indium tin oxide (ITO) coated glass slides were obtained from Bruker Daltonics (Billerica, MA). .. Generation of matrix pre-coated slides To prepare pre-coated slides, THAP was dissolved in 9:1 ACN: water (v/v) at a concentration of 40 mg/mL and sonicated for 10 min in a sonicator bath (Fisher Scientific, Pittsburgh, PA). .. The matrix solution was then applied to an ITO slide using a TM Sprayer (HTX Technologies, Chapel Hill, NC) at 30 °C, flow rate of 0.05 mL/min, velocity of 1200 mm/min and track spacing of 1.5 mm.

High Performance Liquid Chromatography:

Article Title: N-Linked Glycan Profiling of Mature Human Milk by High Performance Microfluidic Chip Liquid Chromatography Time-of-Flight Tandem Mass Spectrometry
Article Snippet: The extraction was performed with an automated Gilson GX-274 ASPEC liquid handling robot controlled by Trilution software (Middleton, WI). .. The extraction method was programmed as follows: Cartridges were first washed with 6 ml nanopure water, then conditioned with 6 ml 80% acetonitrile (ACN) (Fisher Scientific, HPLC grade), 0.1% trifluoroacetic acid (TFA) (Fisher, reagent grade) in water (v/v) and then washed again with 12 ml of nanopure water to remove the ACN. .. After conditioning, the N-glycan samples were loaded onto each cartridge and washed with 12 ml of nanopure water at a flow rate of approximately 1 ml/min to remove excess salts.

Article Title: Method development for the certification of a ginsenoside calibration solution via liquid chromatography with absorbance and mass spectrometric detection
Article Snippet: .. HPLC grade acetonitrile (ACN), methanol (MeOH), water (H2 O), and 0.1 % (v/v) formic acid (FA) in H2 O were purchased from Fisher Scientific (Pittsburgh, PA, USA). ..

Lysis:

Article Title: Anti-Inflammatory Impact of Minocycline in a Mouse Model of Tauopathy
Article Snippet: .. Cytokine antibody arrays Frozen cortical tissue was homogenized in lysis buffer (50 mM TBS [pH 7.4], 2 mM ethylene glycol tetraacetic acid (EGTA), 1 mM phenylmethylsulphonylfluoride (PMSF), 1 mM sodium orthovanadate, 1 mM sodium fluoride, and protease inhibitor cocktail) using a mechanical homogenizer (Polytron; Fisher Scientific Ltd., Loughborough, UK) at 100 mg/ml (w/v). ..

Protease Inhibitor:

Article Title: Anti-Inflammatory Impact of Minocycline in a Mouse Model of Tauopathy
Article Snippet: .. Cytokine antibody arrays Frozen cortical tissue was homogenized in lysis buffer (50 mM TBS [pH 7.4], 2 mM ethylene glycol tetraacetic acid (EGTA), 1 mM phenylmethylsulphonylfluoride (PMSF), 1 mM sodium orthovanadate, 1 mM sodium fluoride, and protease inhibitor cocktail) using a mechanical homogenizer (Polytron; Fisher Scientific Ltd., Loughborough, UK) at 100 mg/ml (w/v). ..

Low Protein Binding:

Article Title: Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains
Article Snippet: Samples were added and passed through the resin, followed by a rinse with 1 mL 0.1% v/v TFA/5% v/v acetonitrile (ACN, Sigma) in water. .. Peptides were eluted from SPE columns into clean 1.5 mL low-protein binding tubes (Fisher Scientific) using 1 mL 0.1% v/v TFA/80% v/v ACN in water. .. ACN was removed and samples brought to ~100 μL using a Vacufuge Plus (Eppendorf).

Electrophoresis:

Article Title: Real-time fluorescence assays to monitor duplex unwinding and ATPase activities of helicase proteins
Article Snippet: Phenol/choloroform/isoamyl alcohol (25:24:1) (Ambion, cat. no. 15593031). .. Spermidine (Sigma-Aldrich, cat. no. S2626) ATP (Sigma-Aldrich, cat. no. A7699) CTP (Sigma-Aldrich, cat. no. C1506) GTP (Sigma-Aldrich, cat. no. G8877) UTP (Sigma-Aldrich, cat. no. U6875) Urea Electrophoresis Grade (Sigma-Aldrich, cat. no. U6504) Acrylamide/Bis-acrylamide (40% w/v; 29:1 ratio) (Fisher Scientific, cat. no. BP14081) Ammonium persulfate (Bio-Rad, cat. no. 161-0700) TEMED (Sigma-Aldrich, cat. no. T9281). .. Boric acid (Fisher Scientific, cat. no. A74-1) EDTA (Sigma-Aldrich, cat. no. EDS-500G).

Staining:

Article Title: Threonine 89 Is an Important Residue of Profilin-1 That Is Phosphorylatable by Protein Kinase A
Article Snippet: PKA treated His-tagged Pfn1 was first subjected to SDS-PAGE. .. The gels were washed twice with MilliQ water for 5 minutes each and then stained with Coomassie blue [0.1% (w/v) R250 (Fisher Scientific, Waltham, MA, USA), 40% (v/v) ethanol (Decon, King of Prussia, PA, USA), 10% (v/v) Acetic Acid (Fisher Scientific, Waltham, MA, USA)]. .. After destaining gels with a destaining solution [10% (v/v) ethanol, 7.5% (v/v) acetic acid], His-tagged Pfn1 gel band was excised, washed with HPLC water and destained with 50% acetonitrile (ACN)/25mM ammonium bicarbonate until staining was no longer visible.

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    Fisher Scientific acn v v
    Acn V V, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acn v v/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acn v v - by Bioz Stars, 2021-07
    86/100 stars
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