acetonitrile water  (Millipore)


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    Structured Review

    Millipore acetonitrile water
    Acetonitrile Water, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile water/product/Millipore
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    acetonitrile water - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations
    Article Snippet: Analytical LC-MS was performed using a Waters 2695 Separations Module and a Waters 2996 Photodiode Array Detector equipped with a Varian Microsorb C18 column (150 × 2 mm) or a Waters X-Bridge C8 column (150 × 2.1 mm) or a Varian Microsorb C8 column (150 × 2 mm) at a flow rate of 0.2 mL/ min. Preparative HPLC was performed using a Rainin HPXL solvent delivery system equipped with a Rainin UV-1 detector using either a Varian Microsorb 100-5 C18 column (250 × 21.4 mm) or a Waters X-Bridge C8 column (150 × 19 mm) at a flow rate of 16 mL/min. .. Buffer solutions were diluted with acetonitrile/water (1∶4, 0.05% trifluoroacetic acid) to 4 mL total volume in a Millipore centrifugal filter tube.

    Article Title: Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B1 Carry Over in Milk
    Article Snippet: The extract was dried under nitrogen, redissolved in 1 ml acetonitrile∶water (25∶75) solution and filtered (Millipore Corporation; HV 0.45 µm). .. HPLC analysis was performed with a Perkin Elmer LC (Perkin Elmer, Norwalk, CT, USA) equipped with an LC-200 pump and a Jasco FP-1520 fluorescence detector (Jasco, Tokyo, Japan) set at 365 nm (excitation) and 440 nm (emission).

    Article Title: Poly(3-Hydroxybutyrate) Degradation in Ralstonia eutropha H16 Is Mediated Stereoselectively to (S)-3-Hydroxybutyryl Coenzyme A (CoA) via Crotonyl-CoA
    Article Snippet: A Shimadzu HPLC system (Shimadzu, Duisburg, Germany), equipped with Nucleocel DELTA-RP S (5 μm, 250 mm in length; Macherey-Nagel, Düren, Germany), was used for the identification of the stereoisomers. .. Samples of 2 μl were injected into the column, an isocratic flow rate of acetonitrile-water (40/60, vol/vol) at 0.3 ml/min and 17°C for 70 min was performed, and the fluorescent detection occurred at 532 nm using an excitation wavelength of 485 nm. ( R )-3HB and ( S )-3HB (Sigma-Aldrich, Seelze, Germany) were used as standards and processed like the collected 3HB-CoA, starting from the evaporation step in the 40°C vacuum furnace.

    Article Title: In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation
    Article Snippet: The labeled glycans were separated on a normal phase analytical HPLC column (GlykoSepN, ProZyme) by using 50 mM ammonium formate (pH 4.4) (solvent A) and acetonitrile (solvent B) as eluents. .. The peaks were detected by using a fluorescence detector (λex = 330 nm and λem = 420 nm), collected, and characterized with MALDI-MS by using a matrix composed of 2,5-dihydrobenzoic acid in acetonitrile/water with Nafion perfluorinated resin (Aldrich) and trifluoroacetic acid as additives.

    Article Title: The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression
    Article Snippet: .. Specifically, 2 volumes of the SCFA extracts were mixed with 1 volume of 200 mM 3-nitrophenylhydrazine hydrochloride in acetonitrile-water (1:1, v/v) and 1 volume of 120 mM N-(3-dimethylaminopropyl)-N”-ethylcarbodiimide hydrochloride (Sigma # 03449 HPLC) in acetonitrile-water (1:1, v/v) containing 6% pyridine (Sigma # 270407). .. The reactions were incubated at 40C for 30 min and then diluted to 1 mL with acetonitrile.

    Article Title: Regulation of Fumonisin Biosynthesis in Fusarium verticillioides by a Zinc Binuclear Cluster-Type Gene, ZFR1 †
    Article Snippet: .. The fumonisins were eluted with 2 ml of acetonitrile-water (7:3, vol/vol), derivatized with o -phthaldehyde (Sigma Chemical Co.), and analyzed with an HPLC apparatus (Shimadzu Scientific Instruments, Inc., Kyoto, Japan) equipped with an analytical C18 column (150 by 4.6 mm) and a variable-wavelength spectrofluorometric detector (excitation wavelength, 335 nm; emission wavelength, 440 nm). .. FB1 was quantified by comparison with an FB1 standard (Sigma Chemical Co.).

    Flow Cytometry:

    Article Title: Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations
    Article Snippet: Analytical LC-MS was performed using a Waters 2695 Separations Module and a Waters 2996 Photodiode Array Detector equipped with a Varian Microsorb C18 column (150 × 2 mm) or a Waters X-Bridge C8 column (150 × 2.1 mm) or a Varian Microsorb C8 column (150 × 2 mm) at a flow rate of 0.2 mL/ min. Preparative HPLC was performed using a Rainin HPXL solvent delivery system equipped with a Rainin UV-1 detector using either a Varian Microsorb 100-5 C18 column (250 × 21.4 mm) or a Waters X-Bridge C8 column (150 × 19 mm) at a flow rate of 16 mL/min. .. Buffer solutions were diluted with acetonitrile/water (1∶4, 0.05% trifluoroacetic acid) to 4 mL total volume in a Millipore centrifugal filter tube.

    Article Title: Poly(3-Hydroxybutyrate) Degradation in Ralstonia eutropha H16 Is Mediated Stereoselectively to (S)-3-Hydroxybutyryl Coenzyme A (CoA) via Crotonyl-CoA
    Article Snippet: .. Samples of 2 μl were injected into the column, an isocratic flow rate of acetonitrile-water (40/60, vol/vol) at 0.3 ml/min and 17°C for 70 min was performed, and the fluorescent detection occurred at 532 nm using an excitation wavelength of 485 nm. ( R )-3HB and ( S )-3HB (Sigma-Aldrich, Seelze, Germany) were used as standards and processed like the collected 3HB-CoA, starting from the evaporation step in the 40°C vacuum furnace. .. An enzyme assay was performed to analyze the influence of the PHB depolymerase PhaZ1 of R. eutropha on PHB degradation in the presence of different phasins of this bacterium.

    Article Title: In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation
    Article Snippet: A gradient of 20–52% solvent A over 80 min was used at a flow rate of 0.4 ml/min. .. The peaks were detected by using a fluorescence detector (λex = 330 nm and λem = 420 nm), collected, and characterized with MALDI-MS by using a matrix composed of 2,5-dihydrobenzoic acid in acetonitrile/water with Nafion perfluorinated resin (Aldrich) and trifluoroacetic acid as additives.

    Sample Prep:

    Article Title: Poly(3-Hydroxybutyrate) Degradation in Ralstonia eutropha H16 Is Mediated Stereoselectively to (S)-3-Hydroxybutyryl Coenzyme A (CoA) via Crotonyl-CoA
    Article Snippet: Paragraph title: Sample preparation of 3HB-CoA and identification of stereoisomers. ... Samples of 2 μl were injected into the column, an isocratic flow rate of acetonitrile-water (40/60, vol/vol) at 0.3 ml/min and 17°C for 70 min was performed, and the fluorescent detection occurred at 532 nm using an excitation wavelength of 485 nm. ( R )-3HB and ( S )-3HB (Sigma-Aldrich, Seelze, Germany) were used as standards and processed like the collected 3HB-CoA, starting from the evaporation step in the 40°C vacuum furnace.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations
    Article Snippet: Analytical LC-MS was performed using a Waters 2695 Separations Module and a Waters 2996 Photodiode Array Detector equipped with a Varian Microsorb C18 column (150 × 2 mm) or a Waters X-Bridge C8 column (150 × 2.1 mm) or a Varian Microsorb C8 column (150 × 2 mm) at a flow rate of 0.2 mL/ min. Preparative HPLC was performed using a Rainin HPXL solvent delivery system equipped with a Rainin UV-1 detector using either a Varian Microsorb 100-5 C18 column (250 × 21.4 mm) or a Waters X-Bridge C8 column (150 × 19 mm) at a flow rate of 16 mL/min. .. Buffer solutions were diluted with acetonitrile/water (1∶4, 0.05% trifluoroacetic acid) to 4 mL total volume in a Millipore centrifugal filter tube.

    Fluorescence:

    Article Title: Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B1 Carry Over in Milk
    Article Snippet: The extract was dried under nitrogen, redissolved in 1 ml acetonitrile∶water (25∶75) solution and filtered (Millipore Corporation; HV 0.45 µm). .. HPLC analysis was performed with a Perkin Elmer LC (Perkin Elmer, Norwalk, CT, USA) equipped with an LC-200 pump and a Jasco FP-1520 fluorescence detector (Jasco, Tokyo, Japan) set at 365 nm (excitation) and 440 nm (emission).

    Article Title: In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation
    Article Snippet: .. The peaks were detected by using a fluorescence detector (λex = 330 nm and λem = 420 nm), collected, and characterized with MALDI-MS by using a matrix composed of 2,5-dihydrobenzoic acid in acetonitrile/water with Nafion perfluorinated resin (Aldrich) and trifluoroacetic acid as additives. .. PglA, PglH, PglI, and PglJ were overexpressed, purified by using Ni2+ affinity chromatography, and confirmed by SDS/PAGE (Coomassie-stained) and Western blot analysis ( ).

    Ligation:

    Article Title: Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations
    Article Snippet: Buffer solutions were diluted with acetonitrile/water (1∶4, 0.05% trifluoroacetic acid) to 4 mL total volume in a Millipore centrifugal filter tube. .. Native chemical ligation buffer was prepared by dissolving guanidine•HCl (1.146 g, 6M) in water.

    Isolation:

    Article Title: Rapid Lead Discovery Through Iterative Screening of One Bead One Compound Libraries
    Article Snippet: .. These beads were collected and stripped of bound protein by incubating in 50% acetonitrile/water (3 × 5 mL) for 30 min, followed by incubation in acetonitrile for 60 min. Each bead was then isolated into a single well of a 96-well filter plate (Millipore) and washed with water. ..

    Magnetic Beads:

    Article Title: Rapid Lead Discovery Through Iterative Screening of One Bead One Compound Libraries
    Article Snippet: After incubation, beads were gently washed with TBS and incubated again with 1:500 rabbit anti-MMP-14 antibody in TBS for 2 h. The beads were washed again and incubated with 1 mg/mL magnetic beads coated with goat antirabbit secondary antibody. .. These beads were collected and stripped of bound protein by incubating in 50% acetonitrile/water (3 × 5 mL) for 30 min, followed by incubation in acetonitrile for 60 min. Each bead was then isolated into a single well of a 96-well filter plate (Millipore) and washed with water.

    Labeling:

    Article Title: In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation
    Article Snippet: The labeled glycans were separated on a normal phase analytical HPLC column (GlykoSepN, ProZyme) by using 50 mM ammonium formate (pH 4.4) (solvent A) and acetonitrile (solvent B) as eluents. .. The peaks were detected by using a fluorescence detector (λex = 330 nm and λem = 420 nm), collected, and characterized with MALDI-MS by using a matrix composed of 2,5-dihydrobenzoic acid in acetonitrile/water with Nafion perfluorinated resin (Aldrich) and trifluoroacetic acid as additives.

    Evaporation:

    Article Title: Poly(3-Hydroxybutyrate) Degradation in Ralstonia eutropha H16 Is Mediated Stereoselectively to (S)-3-Hydroxybutyryl Coenzyme A (CoA) via Crotonyl-CoA
    Article Snippet: .. Samples of 2 μl were injected into the column, an isocratic flow rate of acetonitrile-water (40/60, vol/vol) at 0.3 ml/min and 17°C for 70 min was performed, and the fluorescent detection occurred at 532 nm using an excitation wavelength of 485 nm. ( R )-3HB and ( S )-3HB (Sigma-Aldrich, Seelze, Germany) were used as standards and processed like the collected 3HB-CoA, starting from the evaporation step in the 40°C vacuum furnace. .. An enzyme assay was performed to analyze the influence of the PHB depolymerase PhaZ1 of R. eutropha on PHB degradation in the presence of different phasins of this bacterium.

    Sequencing:

    Article Title: Aberrant Glycosylation in the Human Trabecular Meshwork
    Article Snippet: To identify the proteins and perform quantification, protein bands separated on 10% SDS-PAGE were excised, the gel slices were destained with 50% acetonitrile/water and were suspended in 0.5 M triethylammoniumbicarbonate (TEAB; number 17902 Sigma Chemical Co., St. Louis, MO) pH 8.5 and reduced with 10 mM Tris-(2-carboxyethyl) phosphine (TCEP; Sigma Chemical Co., St. Louis). .. The proteins were subsequently alkylated in the dark using 55 mM solution of iodoacetamide (Catalog No-RPN6302V; GE Healthcare Inc., Buckinghamshire, England) and in-gel digested with sequencing grade-modified trypsin (catalog number V5113, Promega Corporation, Madison, WI 0.1 μg/15 μl in 15 mM N-ethylmorpholin) overnight at 37°C.

    Article Title: Decreased carbohydrate metabolism enzyme activities in the glaucomatous trabecular meshwork
    Article Snippet: Protein bands thus separated were excised, destained with 50% acetonitrile /water, and suspended in 0.5 M triethylammoniumbicarbonate (TEAB; number 17902; Sigma Chemical Co.) pH 8.5 and reduced with 10 mM Tris-(2-Carboxyethyl) phosphine (TCEP; Sigma Chemical Co.). .. The proteins were subsequently alkylated in the dark using 55 mM solution of iodoacetamide (Catalog No-RPN6302V; GE Healthcare Inc., Buckinghamshire, England) and in-gel digested with sequencing grade modified trypsin (0.1 µg/15 µl in 15 mM N-Ethylmorpholin; catalog number V5113; Promega Corporation, Madison, WI) overnight at 37 °C.

    Incubation:

    Article Title: Poly(3-Hydroxybutyrate) Degradation in Ralstonia eutropha H16 Is Mediated Stereoselectively to (S)-3-Hydroxybutyryl Coenzyme A (CoA) via Crotonyl-CoA
    Article Snippet: After incubation for 4 h at 30°C, the reaction was stopped by acidification with 60 μl of 0.5% (wt/vol) TCA. .. Samples of 2 μl were injected into the column, an isocratic flow rate of acetonitrile-water (40/60, vol/vol) at 0.3 ml/min and 17°C for 70 min was performed, and the fluorescent detection occurred at 532 nm using an excitation wavelength of 485 nm. ( R )-3HB and ( S )-3HB (Sigma-Aldrich, Seelze, Germany) were used as standards and processed like the collected 3HB-CoA, starting from the evaporation step in the 40°C vacuum furnace.

    Article Title: The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression
    Article Snippet: Specifically, 2 volumes of the SCFA extracts were mixed with 1 volume of 200 mM 3-nitrophenylhydrazine hydrochloride in acetonitrile-water (1:1, v/v) and 1 volume of 120 mM N-(3-dimethylaminopropyl)-N”-ethylcarbodiimide hydrochloride (Sigma # 03449 HPLC) in acetonitrile-water (1:1, v/v) containing 6% pyridine (Sigma # 270407). .. The reactions were incubated at 40C for 30 min and then diluted to 1 mL with acetonitrile.

    Article Title: Rapid Lead Discovery Through Iterative Screening of One Bead One Compound Libraries
    Article Snippet: .. These beads were collected and stripped of bound protein by incubating in 50% acetonitrile/water (3 × 5 mL) for 30 min, followed by incubation in acetonitrile for 60 min. Each bead was then isolated into a single well of a 96-well filter plate (Millipore) and washed with water. ..

    Article Title: Aberrant Glycosylation in the Human Trabecular Meshwork
    Article Snippet: To identify the proteins and perform quantification, protein bands separated on 10% SDS-PAGE were excised, the gel slices were destained with 50% acetonitrile/water and were suspended in 0.5 M triethylammoniumbicarbonate (TEAB; number 17902 Sigma Chemical Co., St. Louis, MO) pH 8.5 and reduced with 10 mM Tris-(2-carboxyethyl) phosphine (TCEP; Sigma Chemical Co., St. Louis). .. The extracted peptides were incubated with 8 plex iTRAQ reagents in 0.5 M TEAB containing 60% v/v isopropanol.

    Article Title: Decreased carbohydrate metabolism enzyme activities in the glaucomatous trabecular meshwork
    Article Snippet: Protein bands thus separated were excised, destained with 50% acetonitrile /water, and suspended in 0.5 M triethylammoniumbicarbonate (TEAB; number 17902; Sigma Chemical Co.) pH 8.5 and reduced with 10 mM Tris-(2-Carboxyethyl) phosphine (TCEP; Sigma Chemical Co.). .. The extracted peptides were incubated with the iTRAQ Rg-8-Plex Assay kit reagents (Applied Biosystems, Foster City, CA).

    Blocking Assay:

    Article Title: Rapid Lead Discovery Through Iterative Screening of One Bead One Compound Libraries
    Article Snippet: Screening with First Library (Figure B) After prescreening, all beads were gently washed with TBST, a solution containing 100 nM MMP-14 protein in Starting Block was incubated with all the beads under mild shaking for overnight at 4 °C. .. These beads were collected and stripped of bound protein by incubating in 50% acetonitrile/water (3 × 5 mL) for 30 min, followed by incubation in acetonitrile for 60 min. Each bead was then isolated into a single well of a 96-well filter plate (Millipore) and washed with water.

    Mass Spectrometry:

    Article Title: Decreased carbohydrate metabolism enzyme activities in the glaucomatous trabecular meshwork
    Article Snippet: Paragraph title: Mass spectrometry ... Protein bands thus separated were excised, destained with 50% acetonitrile /water, and suspended in 0.5 M triethylammoniumbicarbonate (TEAB; number 17902; Sigma Chemical Co.) pH 8.5 and reduced with 10 mM Tris-(2-Carboxyethyl) phosphine (TCEP; Sigma Chemical Co.).

    Modification:

    Article Title: Decreased carbohydrate metabolism enzyme activities in the glaucomatous trabecular meshwork
    Article Snippet: Protein bands thus separated were excised, destained with 50% acetonitrile /water, and suspended in 0.5 M triethylammoniumbicarbonate (TEAB; number 17902; Sigma Chemical Co.) pH 8.5 and reduced with 10 mM Tris-(2-Carboxyethyl) phosphine (TCEP; Sigma Chemical Co.). .. The proteins were subsequently alkylated in the dark using 55 mM solution of iodoacetamide (Catalog No-RPN6302V; GE Healthcare Inc., Buckinghamshire, England) and in-gel digested with sequencing grade modified trypsin (0.1 µg/15 µl in 15 mM N-Ethylmorpholin; catalog number V5113; Promega Corporation, Madison, WI) overnight at 37 °C.

    Injection:

    Article Title: Poly(3-Hydroxybutyrate) Degradation in Ralstonia eutropha H16 Is Mediated Stereoselectively to (S)-3-Hydroxybutyryl Coenzyme A (CoA) via Crotonyl-CoA
    Article Snippet: .. Samples of 2 μl were injected into the column, an isocratic flow rate of acetonitrile-water (40/60, vol/vol) at 0.3 ml/min and 17°C for 70 min was performed, and the fluorescent detection occurred at 532 nm using an excitation wavelength of 485 nm. ( R )-3HB and ( S )-3HB (Sigma-Aldrich, Seelze, Germany) were used as standards and processed like the collected 3HB-CoA, starting from the evaporation step in the 40°C vacuum furnace. .. An enzyme assay was performed to analyze the influence of the PHB depolymerase PhaZ1 of R. eutropha on PHB degradation in the presence of different phasins of this bacterium.

    SDS Page:

    Article Title: Aberrant Glycosylation in the Human Trabecular Meshwork
    Article Snippet: .. To identify the proteins and perform quantification, protein bands separated on 10% SDS-PAGE were excised, the gel slices were destained with 50% acetonitrile/water and were suspended in 0.5 M triethylammoniumbicarbonate (TEAB; number 17902 Sigma Chemical Co., St. Louis, MO) pH 8.5 and reduced with 10 mM Tris-(2-carboxyethyl) phosphine (TCEP; Sigma Chemical Co., St. Louis). .. The proteins were subsequently alkylated in the dark using 55 mM solution of iodoacetamide (Catalog No-RPN6302V; GE Healthcare Inc., Buckinghamshire, England) and in-gel digested with sequencing grade-modified trypsin (catalog number V5113, Promega Corporation, Madison, WI 0.1 μg/15 μl in 15 mM N-ethylmorpholin) overnight at 37°C.

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    Millipore milli q water
    Milli Q Water, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/milli q water/product/Millipore
    Average 99 stars, based on 88 article reviews
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    Millipore acetonitrile water
    Acetonitrile Water, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile water/product/Millipore
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
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    Millipore acetonitrile water 50 50 elution
    Acetonitrile Water 50 50 Elution, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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