acetonitrile acn  (Millipore)


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    Structured Review

    Millipore acetonitrile acn
    Acetonitrile Acn, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile acn/product/Millipore
    Average 99 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    acetonitrile acn - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Characterization of the Placental Macrophage Secretome: Implications for Antiviral Activity
    Article Snippet: Unbound proteins were removed by washing with binding buffer and HPLC-grade water. .. A 50% solution of SPA was prepared using a solvent that contains 100% acetonitrile (ACN) (Sigma), 100% isopropanol (Fisher, Pittsburgh, PA), 1% trifluoroacetic acid (TFA, Sigma), and 10% Triton X-100.

    Article Title: Ozone induced dissociation on a traveling wave high resolution mass spectrometer for determination of double bond position in lipids
    Article Snippet: .. HPLC grade methanol, acetonitrile (ACN), and isopropanol (IPA) were purchased from Sigma-Aldrich (St. Louis, MO). .. LC-MS grade ammonium formate and formic acid were provided by Fisher Scientific (Pittsburgh, PA).

    Article Title: Magnetic Carbon Nanocomposites as A MALDI co-Matrix Enhancing MS-Based Glycomics
    Article Snippet: .. HPLC grade ethanol, acetonitrile (ACN), and water were used for sample preparation and were purchased from Sigma-Aldrich (St. Louis, MO). .. CNPs@Fe3 O4 NCs with 2:1 weight ratio of CNPs:Fe3 O4 were synthesized using the modified coprecipitation method [ ].

    Binding Assay:

    Article Title: Characterization of the Placental Macrophage Secretome: Implications for Antiviral Activity
    Article Snippet: Unbound proteins were removed by washing with binding buffer and HPLC-grade water. .. A 50% solution of SPA was prepared using a solvent that contains 100% acetonitrile (ACN) (Sigma), 100% isopropanol (Fisher, Pittsburgh, PA), 1% trifluoroacetic acid (TFA, Sigma), and 10% Triton X-100.

    Sample Prep:

    Article Title: Magnetic Carbon Nanocomposites as A MALDI co-Matrix Enhancing MS-Based Glycomics
    Article Snippet: .. HPLC grade ethanol, acetonitrile (ACN), and water were used for sample preparation and were purchased from Sigma-Aldrich (St. Louis, MO). .. CNPs@Fe3 O4 NCs with 2:1 weight ratio of CNPs:Fe3 O4 were synthesized using the modified coprecipitation method [ ].

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Top-down Mass Spectrometry Analysis of Human Serum Autoantibody Antigen-Binding Fragments
    Article Snippet: .. Materials and Reagents LC/MS CHROMASOLV® grade isopropanol (IPA), acetonitrile (ACN), LC-MS grade water, acetic acid (HAc), and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO). .. Pierce™ Trifluoroacetic Acid (TFA), Bond-Breaker™ TCEP solution, Protein A/agarose beads, and papain were obtained from ThermoFisher Scientific (Hanover Park, IL).

    Article Title: Two-Dimensional Separation Using High-pH and Low-pH Reversed Phase Liquid Chromatography for Top-down Proteomics
    Article Snippet: .. LC/MS CHROMASOLV® grade isopropanol (IPA), acetonitrile (ACN), and water were purchased from Sigma-Aldrich (St. Louis, MO). .. Analytical reagent (AR) grade ammonium formate (AF) and acetic acid (HAc) were also procured from Sigma-Aldrich.

    Article Title: Ozone induced dissociation on a traveling wave high resolution mass spectrometer for determination of double bond position in lipids
    Article Snippet: HPLC grade methanol, acetonitrile (ACN), and isopropanol (IPA) were purchased from Sigma-Aldrich (St. Louis, MO). .. LC-MS grade ammonium formate and formic acid were provided by Fisher Scientific (Pittsburgh, PA).

    Article Title: Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome
    Article Snippet: .. Methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), ammonium formate, and formic acid were of LC-MS quality and obtained from Sigma-Aldrich (Sigma-Aldrich GmbH, Taufkirchen, Germany). .. The water was purged through a Merck Millipore system with a resistance of 18 MΩ and a total organic carbon (TOC) of < 4 ppb.

    Article Title: Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry at negative mode
    Article Snippet: .. The LC-MS grade methanol (MeOH), acetonitrile (ACN), isopropanol alchohol (IPA), formic acid (HCOOH), ammonium formate (HCOONH4 ), ammnonium acetate (CH3 COONH4 ), ammonium bicarbonate (NH4 HCO3 ), ammonium hydroxide (NH3 .H2 O) were obtained from Sigma-Aldrich (St. Louis, MO, USA). .. Ultra-pure water was obtained by using a Milli-Q system (Millipore, Bedford, USA).

    Biomarker Assay:

    Article Title: Characterization of the Placental Macrophage Secretome: Implications for Antiviral Activity
    Article Snippet: A 50% solution of SPA was prepared using a solvent that contains 100% acetonitrile (ACN) (Sigma), 100% isopropanol (Fisher, Pittsburgh, PA), 1% trifluoroacetic acid (TFA, Sigma), and 10% Triton X-100. .. Mass spectra were collected with the Protein Chip system in the range of 5000–20,000 m/z and peaks were analyzed using the Biomarker Wizard provided within the Protein Chip® software 3.2.

    BIA-KA:

    Article Title: Two-Dimensional Separation Using High-pH and Low-pH Reversed Phase Liquid Chromatography for Top-down Proteomics
    Article Snippet: LC/MS CHROMASOLV® grade isopropanol (IPA), acetonitrile (ACN), and water were purchased from Sigma-Aldrich (St. Louis, MO). .. Pierce™ Trifluoroacetic Acid (TFA), formic acid (FA), and the Pierce™ BCA Protein Assay Kit were obtained from Thermo Scientific (Hanover Park, IL).

    Purification:

    Article Title: Monitoring antigenic protein integrity during glycoconjugate vaccine synthesis using capillary electrophoresis-mass spectrometry
    Article Snippet: Ammonium hydroxide (30 % solution), acetic acid, formic acid, isopropanol (IPA), acetonitrile (ACN), polybrene (hexadimethrine bromide, PB; average molecular weight (MW) 15,000), dextran sulfate (DS, average MW > 500,000), benzamidine hydrochloride, sodium tetraborate and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Deionized water was obtained from a Milli-Q purification system (Millipore, Bedford, MA, USA).

    HAC Assay:

    Article Title: Top-down Mass Spectrometry Analysis of Human Serum Autoantibody Antigen-Binding Fragments
    Article Snippet: .. Materials and Reagents LC/MS CHROMASOLV® grade isopropanol (IPA), acetonitrile (ACN), LC-MS grade water, acetic acid (HAc), and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO). .. Pierce™ Trifluoroacetic Acid (TFA), Bond-Breaker™ TCEP solution, Protein A/agarose beads, and papain were obtained from ThermoFisher Scientific (Hanover Park, IL).

    Article Title: Two-Dimensional Separation Using High-pH and Low-pH Reversed Phase Liquid Chromatography for Top-down Proteomics
    Article Snippet: LC/MS CHROMASOLV® grade isopropanol (IPA), acetonitrile (ACN), and water were purchased from Sigma-Aldrich (St. Louis, MO). .. Analytical reagent (AR) grade ammonium formate (AF) and acetic acid (HAc) were also procured from Sigma-Aldrich.

    Indirect Immunoperoxidase Assay:

    Article Title: Top-down Mass Spectrometry Analysis of Human Serum Autoantibody Antigen-Binding Fragments
    Article Snippet: .. Materials and Reagents LC/MS CHROMASOLV® grade isopropanol (IPA), acetonitrile (ACN), LC-MS grade water, acetic acid (HAc), and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO). .. Pierce™ Trifluoroacetic Acid (TFA), Bond-Breaker™ TCEP solution, Protein A/agarose beads, and papain were obtained from ThermoFisher Scientific (Hanover Park, IL).

    Article Title: Two-Dimensional Separation Using High-pH and Low-pH Reversed Phase Liquid Chromatography for Top-down Proteomics
    Article Snippet: .. LC/MS CHROMASOLV® grade isopropanol (IPA), acetonitrile (ACN), and water were purchased from Sigma-Aldrich (St. Louis, MO). .. Analytical reagent (AR) grade ammonium formate (AF) and acetic acid (HAc) were also procured from Sigma-Aldrich.

    Article Title: Ozone induced dissociation on a traveling wave high resolution mass spectrometer for determination of double bond position in lipids
    Article Snippet: .. HPLC grade methanol, acetonitrile (ACN), and isopropanol (IPA) were purchased from Sigma-Aldrich (St. Louis, MO). .. LC-MS grade ammonium formate and formic acid were provided by Fisher Scientific (Pittsburgh, PA).

    Article Title: Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome
    Article Snippet: .. Methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), ammonium formate, and formic acid were of LC-MS quality and obtained from Sigma-Aldrich (Sigma-Aldrich GmbH, Taufkirchen, Germany). .. The water was purged through a Merck Millipore system with a resistance of 18 MΩ and a total organic carbon (TOC) of < 4 ppb.

    Article Title: Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry at negative mode
    Article Snippet: .. The LC-MS grade methanol (MeOH), acetonitrile (ACN), isopropanol alchohol (IPA), formic acid (HCOOH), ammonium formate (HCOONH4 ), ammnonium acetate (CH3 COONH4 ), ammonium bicarbonate (NH4 HCO3 ), ammonium hydroxide (NH3 .H2 O) were obtained from Sigma-Aldrich (St. Louis, MO, USA). .. Ultra-pure water was obtained by using a Milli-Q system (Millipore, Bedford, USA).

    Article Title: Monitoring antigenic protein integrity during glycoconjugate vaccine synthesis using capillary electrophoresis-mass spectrometry
    Article Snippet: .. Ammonium hydroxide (30 % solution), acetic acid, formic acid, isopropanol (IPA), acetonitrile (ACN), polybrene (hexadimethrine bromide, PB; average molecular weight (MW) 15,000), dextran sulfate (DS, average MW > 500,000), benzamidine hydrochloride, sodium tetraborate and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Deionized water was obtained from a Milli-Q purification system (Millipore, Bedford, MA, USA).

    Incubation:

    Article Title: Characterization of the Placental Macrophage Secretome: Implications for Antiviral Activity
    Article Snippet: Equal amounts of protein (0.05 μg) were applied to each spot, and the bio-processor was incubated for 30 min at room temperature with shaking. .. A 50% solution of SPA was prepared using a solvent that contains 100% acetonitrile (ACN) (Sigma), 100% isopropanol (Fisher, Pittsburgh, PA), 1% trifluoroacetic acid (TFA, Sigma), and 10% Triton X-100.

    other:

    Article Title: Helicity propensity and interaction of synthetic peptides from heptad-repeat domains of herpes simplex virus 1 glycoprotein H: a circular dichroism study.
    Article Snippet: Na 2 HPO 4 , NaH 2 PO 4 , Na 2 SO 4 , KCl, NaCl powder were purchased from Carlo Erba Reagenti (Milan, Italy), while 2,2,2 trifluoroethanol (TFE) and acetonitrile (ACN) were from Fluka (Sigma-Aldrich, Milan, Italy).

    Significance Assay:

    Article Title: Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome
    Article Snippet: In order to exclude overfitting, a cross-validation analysis of variance (CV-ANOVA) was applied for each OPLS-DA classification model. Further, the significance of each model (P value) and indicators such as the goodness-of-fit R 2 Y (cum) and the goodness-of-prediction Q 2 (cum) were subsequently reported. .. Methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), ammonium formate, and formic acid were of LC-MS quality and obtained from Sigma-Aldrich (Sigma-Aldrich GmbH, Taufkirchen, Germany).

    Mass Spectrometry:

    Article Title: Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome
    Article Snippet: In order to identify significant features of the metabolomics data set, a lipidomics-MS/MS approach was applied using an Acquity ultrahigh-performance liquid chromatography system (Waters GmbH, Eschborn, Germany) coupled to a Bruker maXis ultrahigh-resolution–time of flight mass spectrometer (UHR-TOF-MS) (Bruker Daltonik GmbH, Bremen, Germany) as previously described in the work of Witting et al. ( ). .. Methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), ammonium formate, and formic acid were of LC-MS quality and obtained from Sigma-Aldrich (Sigma-Aldrich GmbH, Taufkirchen, Germany).

    Tandem Mass Spectroscopy:

    Article Title: Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome
    Article Snippet: Methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), ammonium formate, and formic acid were of LC-MS quality and obtained from Sigma-Aldrich (Sigma-Aldrich GmbH, Taufkirchen, Germany). .. Chromatograms were averaged, made standard dependent, and calibrated using a reference list of standards of the injected calibration standard mix (G1969-85000; Agilent, Waldbronn, Germany), as well as the standards used for the MS/MS experiment with an error of less than 0.5 ppm.

    Injection:

    Article Title: Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome
    Article Snippet: Methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), ammonium formate, and formic acid were of LC-MS quality and obtained from Sigma-Aldrich (Sigma-Aldrich GmbH, Taufkirchen, Germany). .. Chromatograms were averaged, made standard dependent, and calibrated using a reference list of standards of the injected calibration standard mix (G1969-85000; Agilent, Waldbronn, Germany), as well as the standards used for the MS/MS experiment with an error of less than 0.5 ppm.

    Recombinant:

    Article Title: Monitoring antigenic protein integrity during glycoconjugate vaccine synthesis using capillary electrophoresis-mass spectrometry
    Article Snippet: Ammonium hydroxide (30 % solution), acetic acid, formic acid, isopropanol (IPA), acetonitrile (ACN), polybrene (hexadimethrine bromide, PB; average molecular weight (MW) 15,000), dextran sulfate (DS, average MW > 500,000), benzamidine hydrochloride, sodium tetraborate and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. The TB10.4 and Ag85B immunogenic proteins were obtained as recombinant forms in Escherichia coli as reported by Piubelli et al. [ ] and finally collected in 20 mM 3-(N -morpholino)propanesulfonic acid (MOPS) and 0.4 M NaCl, pH 7.0 at different concentrations.

    Chromatin Immunoprecipitation:

    Article Title: Characterization of the Placental Macrophage Secretome: Implications for Antiviral Activity
    Article Snippet: Cationic proteins bind to the CM10 chip while metal (copper)-binding proteins bind to the IMAC30 chip. .. A 50% solution of SPA was prepared using a solvent that contains 100% acetonitrile (ACN) (Sigma), 100% isopropanol (Fisher, Pittsburgh, PA), 1% trifluoroacetic acid (TFA, Sigma), and 10% Triton X-100.

    Liquid Chromatography:

    Article Title: Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome
    Article Snippet: In order to identify significant features of the metabolomics data set, a lipidomics-MS/MS approach was applied using an Acquity ultrahigh-performance liquid chromatography system (Waters GmbH, Eschborn, Germany) coupled to a Bruker maXis ultrahigh-resolution–time of flight mass spectrometer (UHR-TOF-MS) (Bruker Daltonik GmbH, Bremen, Germany) as previously described in the work of Witting et al. ( ). .. Methanol (MeOH), acetonitrile (ACN), isopropanol (IPA), ammonium formate, and formic acid were of LC-MS quality and obtained from Sigma-Aldrich (Sigma-Aldrich GmbH, Taufkirchen, Germany).

    Molecular Weight:

    Article Title: Monitoring antigenic protein integrity during glycoconjugate vaccine synthesis using capillary electrophoresis-mass spectrometry
    Article Snippet: .. Ammonium hydroxide (30 % solution), acetic acid, formic acid, isopropanol (IPA), acetonitrile (ACN), polybrene (hexadimethrine bromide, PB; average molecular weight (MW) 15,000), dextran sulfate (DS, average MW > 500,000), benzamidine hydrochloride, sodium tetraborate and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Deionized water was obtained from a Milli-Q purification system (Millipore, Bedford, MA, USA).

    Software:

    Article Title: Characterization of the Placental Macrophage Secretome: Implications for Antiviral Activity
    Article Snippet: A 50% solution of SPA was prepared using a solvent that contains 100% acetonitrile (ACN) (Sigma), 100% isopropanol (Fisher, Pittsburgh, PA), 1% trifluoroacetic acid (TFA, Sigma), and 10% Triton X-100. .. Mass spectra were collected with the Protein Chip system in the range of 5000–20,000 m/z and peaks were analyzed using the Biomarker Wizard provided within the Protein Chip® software 3.2.

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  • News
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  • Team
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  • Bioz Stars
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  • 99
    Millipore acetonitrile acn
    Acetonitrile Acn, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile acn/product/Millipore
    Average 99 stars, based on 165 article reviews
    Price from $9.99 to $1999.99
    acetonitrile acn - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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