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Agilent technologies acetonitrile acn
Acetonitrile Acn, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acetonitrile acn/product/Agilent technologies
Average 92 stars, based on 11 article reviews
Price from $9.99 to $1999.99
acetonitrile acn - by Bioz Stars, 2020-07
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Analysis of Human Blood Plasma Proteome from Ten Healthy Volunteers from Indian Population
Article Snippet: .. b) Prefractionation at peptide level by SCX chromatographic separation Tryptic digest from a total of 320 µg protein was resuspended in 1 ml of buffer A [10 mM KH2PO4, 25% (v/v) acetonitrile (ACN), pH 2.9] and separated on a SCX column (Zorbax 300-SCX, 5 µm, 4.5 mm ID × 100 mm, Agilent Technologies, Santa Clara, CA, USA) on Agilent 1100 series HPLC ( ). .. The conditions for SCX fractionation include: flow rate 700 µl/min and 40 min gradient- 5 min, 0−5% buffer B (buffer A+1 M KCl); 5 min, 5–10%; 5 min, 10–23%; 5 min, 23–50%; 10 min, 50–100%; 10 min, 100% B.

Article Title: Enhanced Delivery of Imatinib into Vaginal Mucosa via a New Positively Charged Nanocrystal-Loaded in Situ Hydrogel Formulation for Treatment of Cervical Cancer
Article Snippet: .. Characterization of NC, NC@PDA, and NC@PDA-NH2 For determination of drug loading (DL) and encapsulation efficiency (EE), lyophilized nanocrystals with a premeasured mass were dissolved in acetonitrile (ACN), filtered with a 0.45-μm syringe filter, diluted if necessary, and submitted to HPLC analysis on an Agilent 1100 HPLC system (Palo Alto, CA, USA) equipped with an Xtimate ® C18 column (25 cm × 4.6 mm, particle size: 5 μm; Welch Technology, Shanghai, China) with a mobile phase 45:55 (v /v ) mixture of ACN/ammonium acetate buffer solution (10 mM, pH 10) at a flow rate 0.7 mL/min and a detection wavelength of 272 nm. ..

Article Title: Constant illumination reduces circulating melatonin and impairs immune function in the cricket Teleogryllus commodus
Article Snippet: .. The dried sample was resuspended in 50 µl of acetonitrile (ACN), vortexed for 15 min, centrifuged at 9,800 g, 4 °C for 10 min, and the supernatant transferred to a High Performance Liquid Chromatography (HPLC) sample vial (Agilent Technologies, Mulgrave, VIC, Australia). .. Pooled biological quality control samples (PBQC) were prepared by pooling the final supernatant of ten haemolymph samples to form a master mix and then re-aliquoted (25 µl) into HPLC sample vials to generate individual PBQC samples.

Hydrophilic Interaction Liquid Chromatography:

Article Title: Lactate Metabolism is Associated with Mammalian Mitochondria
Article Snippet: .. The column was used in hydrophilic interaction (HILIC) mode with the following buffers and gradients: A = 95% water, 5% acetonitrile (ACN), 10 mM ammonium hydroxide (NH4 OH), 10 mM ammonium acetate (NH4 Ac), pH 9.5; B = 95 % ACN, 5% water; 100% B from 0–3 min, 100% to 0% B from 3–37 min and 0% B from 37–40 min. MS detection was carried out on an Agilent 6540 Q-TOF in negative ESI (electrospray ionization) mode with a mass range of 30–1500 Da. ..

Flow Cytometry:

Article Title: Enhanced Delivery of Imatinib into Vaginal Mucosa via a New Positively Charged Nanocrystal-Loaded in Situ Hydrogel Formulation for Treatment of Cervical Cancer
Article Snippet: .. Characterization of NC, NC@PDA, and NC@PDA-NH2 For determination of drug loading (DL) and encapsulation efficiency (EE), lyophilized nanocrystals with a premeasured mass were dissolved in acetonitrile (ACN), filtered with a 0.45-μm syringe filter, diluted if necessary, and submitted to HPLC analysis on an Agilent 1100 HPLC system (Palo Alto, CA, USA) equipped with an Xtimate ® C18 column (25 cm × 4.6 mm, particle size: 5 μm; Welch Technology, Shanghai, China) with a mobile phase 45:55 (v /v ) mixture of ACN/ammonium acetate buffer solution (10 mM, pH 10) at a flow rate 0.7 mL/min and a detection wavelength of 272 nm. ..

Article Title: An Enhanced Mass Spectrometry Approach Reveals Human Embryonic Stem Cell Growth Factors in Culture
Article Snippet: .. For the multi-dimensional protein identification technology (MuD-PIT) analysis, desalted tryptic peptides (solid phase extraction) in 10% formic acid (FA) were loaded on a strong cation exchange column (Bio SCX Series II, 0.8 × 50 mm; Agilent) in 5% acetonitrile (ACN), 0.1% FA, collecting the flow-through as the first fraction. .. Twelve more fractions were created by sequentially injecting 20 μl of the following KCl fractions in 0.1% FA and collecting the flow-through: 7.5, 15, 30, 45, 60, 75, 90, 120, 150, 300, 500 m m KCl in 5% ACN and 500 m m KCl in 30% ACN.

Spectroscopy:

Article Title: Effects of 5-Hydroxymethylcytosine Epigenetic Modification on the Stability and Molecular Recognition of VEGF i-Motif and G-Quadruplex Structures
Article Snippet: .. UV Visual Spectroscopy The ECD thermal melt profiles of the iM oligonucleotides (2–5 μ M) in 30 mM cacodylate buffers at varying pHs (5.4–8.0) with and without various amounts of KCl and acetonitrile (ACN) were validated using absorbance readings at 260 nm collected on Cary 100 UV-Visible Spectrometer (Agilent Technologies, Santa Clara, CA); the pH of each sample was measured in a pH meter immediately before placement in the spectrophotometer. ..

Chromatography:

Article Title: “Direct Dripping”: A High-Temperature, High-Formaldehyde Emission Electronic Cigarette Use Method
Article Snippet: .. Volatile aldehydes were determined by extracting the DNPH-coated cartridges in acetonitrile (ACN) and analyzing the solution by high-performance liquid chromatography-mass spectrometry (LC/MSD Trap XCT, Agilent Technologies, Santa Clara, CA). .. In separate experiments, heater coil temperatures were measured using an infrared camera (ThermaCAM 560, FLIR Systems, Wilsonville, OR) and a home-built reverse-puffing apparatus which allowed the mouthpiece view to remain unobstructed for the infrared camera ( ).

Mass Spectrometry:

Article Title: Lactate Metabolism is Associated with Mammalian Mitochondria
Article Snippet: .. The column was used in hydrophilic interaction (HILIC) mode with the following buffers and gradients: A = 95% water, 5% acetonitrile (ACN), 10 mM ammonium hydroxide (NH4 OH), 10 mM ammonium acetate (NH4 Ac), pH 9.5; B = 95 % ACN, 5% water; 100% B from 0–3 min, 100% to 0% B from 3–37 min and 0% B from 37–40 min. MS detection was carried out on an Agilent 6540 Q-TOF in negative ESI (electrospray ionization) mode with a mass range of 30–1500 Da. ..

Article Title: LKB1 is a central regulator of tumor initiation and pro-growth metabolism in ErbB2-mediated breast cancer
Article Snippet: .. Analysis of metabolites by liquid chromatography and mass spectrometry (LC-MS) Tumor samples (50 mg), or cell lines cultured for 24 hours, were extracted in a solution of 50% acetonitrile (ACN) and injected onto an Agilent 6430 triple quadrupole LC-MS system for targeted metabolite analysis (ATP, ADP, AMP, creatine, glucose, and lactate). .. Chromatography was performed using a 1290 Infinity ultra-performance liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA) consisting of vacuum degasser, auto-sampler and a binary pump.

Cell Culture:

Article Title: LKB1 is a central regulator of tumor initiation and pro-growth metabolism in ErbB2-mediated breast cancer
Article Snippet: .. Analysis of metabolites by liquid chromatography and mass spectrometry (LC-MS) Tumor samples (50 mg), or cell lines cultured for 24 hours, were extracted in a solution of 50% acetonitrile (ACN) and injected onto an Agilent 6430 triple quadrupole LC-MS system for targeted metabolite analysis (ATP, ADP, AMP, creatine, glucose, and lactate). .. Chromatography was performed using a 1290 Infinity ultra-performance liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA) consisting of vacuum degasser, auto-sampler and a binary pump.

Spectrophotometry:

Article Title: Effects of 5-Hydroxymethylcytosine Epigenetic Modification on the Stability and Molecular Recognition of VEGF i-Motif and G-Quadruplex Structures
Article Snippet: .. UV Visual Spectroscopy The ECD thermal melt profiles of the iM oligonucleotides (2–5 μ M) in 30 mM cacodylate buffers at varying pHs (5.4–8.0) with and without various amounts of KCl and acetonitrile (ACN) were validated using absorbance readings at 260 nm collected on Cary 100 UV-Visible Spectrometer (Agilent Technologies, Santa Clara, CA); the pH of each sample was measured in a pH meter immediately before placement in the spectrophotometer. ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: LKB1 is a central regulator of tumor initiation and pro-growth metabolism in ErbB2-mediated breast cancer
Article Snippet: .. Analysis of metabolites by liquid chromatography and mass spectrometry (LC-MS) Tumor samples (50 mg), or cell lines cultured for 24 hours, were extracted in a solution of 50% acetonitrile (ACN) and injected onto an Agilent 6430 triple quadrupole LC-MS system for targeted metabolite analysis (ATP, ADP, AMP, creatine, glucose, and lactate). .. Chromatography was performed using a 1290 Infinity ultra-performance liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA) consisting of vacuum degasser, auto-sampler and a binary pump.

Injection:

Article Title: LKB1 is a central regulator of tumor initiation and pro-growth metabolism in ErbB2-mediated breast cancer
Article Snippet: .. Analysis of metabolites by liquid chromatography and mass spectrometry (LC-MS) Tumor samples (50 mg), or cell lines cultured for 24 hours, were extracted in a solution of 50% acetonitrile (ACN) and injected onto an Agilent 6430 triple quadrupole LC-MS system for targeted metabolite analysis (ATP, ADP, AMP, creatine, glucose, and lactate). .. Chromatography was performed using a 1290 Infinity ultra-performance liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA) consisting of vacuum degasser, auto-sampler and a binary pump.

Liquid Chromatography:

Article Title: LKB1 is a central regulator of tumor initiation and pro-growth metabolism in ErbB2-mediated breast cancer
Article Snippet: .. Analysis of metabolites by liquid chromatography and mass spectrometry (LC-MS) Tumor samples (50 mg), or cell lines cultured for 24 hours, were extracted in a solution of 50% acetonitrile (ACN) and injected onto an Agilent 6430 triple quadrupole LC-MS system for targeted metabolite analysis (ATP, ADP, AMP, creatine, glucose, and lactate). .. Chromatography was performed using a 1290 Infinity ultra-performance liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA) consisting of vacuum degasser, auto-sampler and a binary pump.

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  • 92
    Agilent technologies acetonitrile acn
    Acetonitrile Acn, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile acn/product/Agilent technologies
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    acetonitrile acn - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Agilent technologies buffer a
    Buffer A, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer a/product/Agilent technologies
    Average 93 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    buffer a - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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