acetonitrile acn trifluoroacetic acid  (Millipore)


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    Name:
    Acetonitrile with 0 1 trifluoroacetic acid
    Description:

    Catalog Number:
    l081000
    Price:
    None
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    Structured Review

    Millipore acetonitrile acn trifluoroacetic acid

    https://www.bioz.com/result/acetonitrile acn trifluoroacetic acid/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acetonitrile acn trifluoroacetic acid - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: LncRNA HOXA-AS3 confers cisplatin resistance by interacting with HOXA3 in non-small-cell lung carcinoma cells
    Article Snippet: Mass spectrometry Protein (0.5 μL) obtained by centrifugation was uniformly coated on the 96-well target plate and dried at room temperature. .. Each well was then overlaid with 0.5 μL α-cyano-4-hydroxycinnamic acid (Fluka) saturated with a 50:47.5:2.5 mixture of acetonitrile:water:trifluoroacetic acid (Sigma, Ameican).

    Cytotoxicity Assay:

    Article Title: Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components
    Article Snippet: Materials RPMI 1640, l -glutamine, streptomycin, penicillin, glutaraldehyde, lipopolysaccharide (LPS), Phorbol 12-myristate 13-acetate (PMA), Tris(2-Carboxyethyl)phosphine hydrochloride solution (TCEP), iodoacetamide (IAA), Bradford reagent, BSA, acetonitrile (ACN) trifluoroacetic acid (≥99.5 %, TFA), and chitosan (with > 75 % deacetylation, #417963), were purchased from Sigma-Aldrich (Sigma, St. Louis, MI, USA). .. While trypsin, the LDH detection kit CytoTox 96® Non-Radioactive Cytotoxicity Assay, and the CellTiterBlue® (CTB) Cell Viability Assay were purchased from Promega (Madison, WI, USA).

    Electrophoresis:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: Paragraph title: Preparative isolation of nuclear protein/32 P-hFIX-AIE RNA complex by solution-phase isoelectric focusing (IEF) and two dimensional electrophoresis (2DE) ... Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

    Stripping Membranes:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: Its IEF (the first dimension) was performed by using an IPG Runner system with a pre-casted ZOOM strip of an appropriate pH range gradient, followed by SDS-PAGE using ZOOM gels. .. Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

    GPC Assay:

    Article Title: Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro
    Article Snippet: The hydrolysate at a concentration of 2 mg/mL in acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) was filtered with a syringe filter (0.45 μm, Millipore) prior to injection. .. First, 50 μL of the filtrate was loaded into a Shimadzu gel permeation chromatography (GPC) system (Japan) equipped with an LC-20AD pump (Shimadzu, Japan), and a diode array UV detector (SPD-M20A, Shimadzu, Japan).

    Mass Spectrometry:

    Article Title: LncRNA HOXA-AS3 confers cisplatin resistance by interacting with HOXA3 in non-small-cell lung carcinoma cells
    Article Snippet: Paragraph title: Mass spectrometry ... Each well was then overlaid with 0.5 μL α-cyano-4-hydroxycinnamic acid (Fluka) saturated with a 50:47.5:2.5 mixture of acetonitrile:water:trifluoroacetic acid (Sigma, Ameican).

    Article Title: Histology-directed and imaging mass spectrometry: an emerging technology in ectopic calcification
    Article Snippet: Paragraph title: 2.5.1 Tissue profiling by MALDI MS ... On a 8 μm-cryosection mounted on ITO conductive glass slide, 2 different areas of interest, papillary and reticular dermis (calcified and non calcified), were targeted for repeated deposition of matrix made of Sinapinic acid (20 mg/mL) in 1:1 acetonitrile/trifluoroacetic-acid (Sigma Aldrich, St. Louis, MO, USA) at 0.1% (v/v) aq.) that was deposited over a series of 6 iterations at 10 droplets (120nL) per iteration.

    Electron Microscopy:

    Article Title: Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components
    Article Snippet: Materials RPMI 1640, l -glutamine, streptomycin, penicillin, glutaraldehyde, lipopolysaccharide (LPS), Phorbol 12-myristate 13-acetate (PMA), Tris(2-Carboxyethyl)phosphine hydrochloride solution (TCEP), iodoacetamide (IAA), Bradford reagent, BSA, acetonitrile (ACN) trifluoroacetic acid (≥99.5 %, TFA), and chitosan (with > 75 % deacetylation, #417963), were purchased from Sigma-Aldrich (Sigma, St. Louis, MI, USA). .. With the following materials purchased elsewhere: HEPES and FCS (PAA, Pasching, Austria), SYBR Green Supermix (Bio-Rad), RevertAid H Minus M-MulV reverse transcriptase (MBI Fermentas, St. Leon-Roth, Germany), TRIzol reagent (Invitrogen), CCL2, TNF-α and IL-6 ELISA kits (PeproTech), IL-1-β ELISA (R & D Systems), caspase-1 inhibitor Ac-YVAD-CMK (Calbiochem), epon (Agar Scientific), osmium tetroxide (Electron Microscopy Sciences, Hatfield, UK), triethylammonium bicarbonate buffer (TEAB) (Fluka, Buchs, Switzerland).

    Flow Cytometry:

    Article Title: Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro
    Article Snippet: The hydrolysate at a concentration of 2 mg/mL in acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) was filtered with a syringe filter (0.45 μm, Millipore) prior to injection. .. The analytical column (Superdex Peptide 10/300 GL, 10×300 mm, 13 mm; GE Healthcare, USA) was eluted with acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) at a flow rate of 0.5 mL/min under the maximum operating pressure of 18 kgf/cm2 and monitored at 214 nm.

    Peptide Mass Fingerprinting:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore). .. PMF analysis was done with a MASCOT server version 2.1 (Matrix Science, London, U.K.) taking the UniProtKB mouse protein database (taxonomy: mus musculus).

    SYBR Green Assay:

    Article Title: Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components
    Article Snippet: Materials RPMI 1640, l -glutamine, streptomycin, penicillin, glutaraldehyde, lipopolysaccharide (LPS), Phorbol 12-myristate 13-acetate (PMA), Tris(2-Carboxyethyl)phosphine hydrochloride solution (TCEP), iodoacetamide (IAA), Bradford reagent, BSA, acetonitrile (ACN) trifluoroacetic acid (≥99.5 %, TFA), and chitosan (with > 75 % deacetylation, #417963), were purchased from Sigma-Aldrich (Sigma, St. Louis, MI, USA). .. With the following materials purchased elsewhere: HEPES and FCS (PAA, Pasching, Austria), SYBR Green Supermix (Bio-Rad), RevertAid H Minus M-MulV reverse transcriptase (MBI Fermentas, St. Leon-Roth, Germany), TRIzol reagent (Invitrogen), CCL2, TNF-α and IL-6 ELISA kits (PeproTech), IL-1-β ELISA (R & D Systems), caspase-1 inhibitor Ac-YVAD-CMK (Calbiochem), epon (Agar Scientific), osmium tetroxide (Electron Microscopy Sciences, Hatfield, UK), triethylammonium bicarbonate buffer (TEAB) (Fluka, Buchs, Switzerland).

    Viability Assay:

    Article Title: Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components
    Article Snippet: Materials RPMI 1640, l -glutamine, streptomycin, penicillin, glutaraldehyde, lipopolysaccharide (LPS), Phorbol 12-myristate 13-acetate (PMA), Tris(2-Carboxyethyl)phosphine hydrochloride solution (TCEP), iodoacetamide (IAA), Bradford reagent, BSA, acetonitrile (ACN) trifluoroacetic acid (≥99.5 %, TFA), and chitosan (with > 75 % deacetylation, #417963), were purchased from Sigma-Aldrich (Sigma, St. Louis, MI, USA). .. While trypsin, the LDH detection kit CytoTox 96® Non-Radioactive Cytotoxicity Assay, and the CellTiterBlue® (CTB) Cell Viability Assay were purchased from Promega (Madison, WI, USA).

    Generated:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: .. Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore). ..

    Imaging:

    Article Title: Visualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry
    Article Snippet: Single slices were thaw-mounted onto an indium tin oxide (ITO) glass slide (conductive glass slides for MALDI imaging, Bruker Daltonics, Bremen, Germany) with a thin layer of ethanol (2 µL, Merck, Germany). .. The MALDI matrix was α-cyano-4-hydroxycinnamic acid (CHCA) (Sigma-Aldrich, Germany) and was prepared at a 7 mg/ml concentration in 50:50 (v/v) acetonitrile:trifluoroacetic acid (0.2%) (Sigma-Aldrich, St. Louis, MO, USA).

    Isolation:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: Paragraph title: Preparative isolation of nuclear protein/32 P-hFIX-AIE RNA complex by solution-phase isoelectric focusing (IEF) and two dimensional electrophoresis (2DE) ... Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

    Injection:

    Article Title: Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro
    Article Snippet: .. The hydrolysate at a concentration of 2 mg/mL in acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) was filtered with a syringe filter (0.45 μm, Millipore) prior to injection. .. First, 50 μL of the filtrate was loaded into a Shimadzu gel permeation chromatography (GPC) system (Japan) equipped with an LC-20AD pump (Shimadzu, Japan), and a diode array UV detector (SPD-M20A, Shimadzu, Japan).

    Molecular Weight:

    Article Title: Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro
    Article Snippet: Paragraph title: Determination of the molecular weight (MW) of the hydrolysate ... The hydrolysate at a concentration of 2 mg/mL in acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) was filtered with a syringe filter (0.45 μm, Millipore) prior to injection.

    Radioactivity:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: The gel was then silver stained and exposed to an X-ray film at 4°C for 1 to 6 days depending on the radioactivity strength. .. Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

    CtB Assay:

    Article Title: Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components
    Article Snippet: Materials RPMI 1640, l -glutamine, streptomycin, penicillin, glutaraldehyde, lipopolysaccharide (LPS), Phorbol 12-myristate 13-acetate (PMA), Tris(2-Carboxyethyl)phosphine hydrochloride solution (TCEP), iodoacetamide (IAA), Bradford reagent, BSA, acetonitrile (ACN) trifluoroacetic acid (≥99.5 %, TFA), and chitosan (with > 75 % deacetylation, #417963), were purchased from Sigma-Aldrich (Sigma, St. Louis, MI, USA). .. While trypsin, the LDH detection kit CytoTox 96® Non-Radioactive Cytotoxicity Assay, and the CellTiterBlue® (CTB) Cell Viability Assay were purchased from Promega (Madison, WI, USA).

    Size-exclusion Chromatography:

    Article Title: Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro
    Article Snippet: Determination of the molecular weight (MW) of the hydrolysate The molecular weights of splenic hydrolysates were determined by gel size exclusion chromatography. .. The hydrolysate at a concentration of 2 mg/mL in acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) was filtered with a syringe filter (0.45 μm, Millipore) prior to injection.

    SDS Page:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: Its IEF (the first dimension) was performed by using an IPG Runner system with a pre-casted ZOOM strip of an appropriate pH range gradient, followed by SDS-PAGE using ZOOM gels. .. Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components
    Article Snippet: Materials RPMI 1640, l -glutamine, streptomycin, penicillin, glutaraldehyde, lipopolysaccharide (LPS), Phorbol 12-myristate 13-acetate (PMA), Tris(2-Carboxyethyl)phosphine hydrochloride solution (TCEP), iodoacetamide (IAA), Bradford reagent, BSA, acetonitrile (ACN) trifluoroacetic acid (≥99.5 %, TFA), and chitosan (with > 75 % deacetylation, #417963), were purchased from Sigma-Aldrich (Sigma, St. Louis, MI, USA). .. With the following materials purchased elsewhere: HEPES and FCS (PAA, Pasching, Austria), SYBR Green Supermix (Bio-Rad), RevertAid H Minus M-MulV reverse transcriptase (MBI Fermentas, St. Leon-Roth, Germany), TRIzol reagent (Invitrogen), CCL2, TNF-α and IL-6 ELISA kits (PeproTech), IL-1-β ELISA (R & D Systems), caspase-1 inhibitor Ac-YVAD-CMK (Calbiochem), epon (Agar Scientific), osmium tetroxide (Electron Microscopy Sciences, Hatfield, UK), triethylammonium bicarbonate buffer (TEAB) (Fluka, Buchs, Switzerland).

    Gel Permeation Chromatography:

    Article Title: Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro
    Article Snippet: The hydrolysate at a concentration of 2 mg/mL in acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) was filtered with a syringe filter (0.45 μm, Millipore) prior to injection. .. First, 50 μL of the filtrate was loaded into a Shimadzu gel permeation chromatography (GPC) system (Japan) equipped with an LC-20AD pump (Shimadzu, Japan), and a diode array UV detector (SPD-M20A, Shimadzu, Japan).

    Concentration Assay:

    Article Title: Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro
    Article Snippet: .. The hydrolysate at a concentration of 2 mg/mL in acetonitrile/water/trifluoroacetic acid (30/70/0.1, v/v/v) was filtered with a syringe filter (0.45 μm, Millipore) prior to injection. .. First, 50 μL of the filtrate was loaded into a Shimadzu gel permeation chromatography (GPC) system (Japan) equipped with an LC-20AD pump (Shimadzu, Japan), and a diode array UV detector (SPD-M20A, Shimadzu, Japan).

    Article Title: Visualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry
    Article Snippet: .. The MALDI matrix was α-cyano-4-hydroxycinnamic acid (CHCA) (Sigma-Aldrich, Germany) and was prepared at a 7 mg/ml concentration in 50:50 (v/v) acetonitrile:trifluoroacetic acid (0.2%) (Sigma-Aldrich, St. Louis, MO, USA). ..

    Two-Dimensional Gel Electrophoresis:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: Paragraph title: Preparative isolation of nuclear protein/32 P-hFIX-AIE RNA complex by solution-phase isoelectric focusing (IEF) and two dimensional electrophoresis (2DE) ... Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

    Staining:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: The gel was then silver stained and exposed to an X-ray film at 4°C for 1 to 6 days depending on the radioactivity strength. .. Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

    Electrofocusing:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein A3 Is the Liver Nuclear Protein Binding to Age Related Increase Element RNA of the Factor IX Gene
    Article Snippet: Paragraph title: Preparative isolation of nuclear protein/32 P-hFIX-AIE RNA complex by solution-phase isoelectric focusing (IEF) and two dimensional electrophoresis (2DE) ... Peptides generated were then recovered by extracting with a solution of acetonitrile:water:trifluoroacetic acid (66:33:0.1 by volume), vacuum-concentrated and cleaned with a Zip Tip C18 (Millipore).

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  • 99
    Millipore trifluoroacetic acid acn tfa
    Trifluoroacetic Acid Acn Tfa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trifluoroacetic acid acn tfa/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trifluoroacetic acid acn tfa - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore acn
    Acn, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acn/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    acn - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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