ace2 gfp  (Sino Biological)


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    Name:
    ACE2 cDNA ORF Clone Human C GFPSpark tag
    Description:
    Full length Clone DNA of Human angiotensin I converting enzyme peptidyl dipeptidase A 2 with C terminal GFPSpark tag
    Catalog Number:
    HG10108-ACG
    Price:
    245.0
    Category:
    cDNA Clone
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Product Aliases:
    ACEH cDNA ORF Clone Human
    Molecule Name:
    ACE2,2010305L05Rik,
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    Structured Review

    Sino Biological ace2 gfp
    ACE2 cDNA ORF Clone Human C GFPSpark tag
    Full length Clone DNA of Human angiotensin I converting enzyme peptidyl dipeptidase A 2 with C terminal GFPSpark tag
    https://www.bioz.com/result/ace2 gfp/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ace2 gfp - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells. .. Single cell cloning was done on these cells to generate cell lines with equivalent expression of ACE2-GFP. .. To detect inhibition of spike binding to ACE2, 2.5 μg/ml S1+S2 ECD-his tagged (Sino Biological, Catalog #40589-V08B1) was incubated with serum collected from vaccinated animals at indicated time points and dilutions on ice for 60 min.

    Article Title: Virus-free and live-cell visualizing SARS-CoV-2 cell entry for studies of neutralizing antibodies and compound inhibitors
    Article Snippet: For lentiviral vectors, the pLVEF1αIHRB-ACE2hR and pLVEF1αmNG vectors were constructed on the pLV-EF1α-MCS-IRES-Bsd vector (Youbio, VT8179). .. The ACE2 cDNA fragment (Sino Biological, HG10108-ACG) linking with an IRES-H2BmRuby3-P2A-BsR DNA fragment (synthesized by Generalbiol, Anhui, China) was cloned in-frame into the XbaI/SalI sites of pLV-EF1α-MCS-IRES-Bsd to obtain the pLVEF1αIHRB-ACE2hR vector. .. Recombinant proteinsRecombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133).

    Expressing:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells. .. Single cell cloning was done on these cells to generate cell lines with equivalent expression of ACE2-GFP. .. To detect inhibition of spike binding to ACE2, 2.5 μg/ml S1+S2 ECD-his tagged (Sino Biological, Catalog #40589-V08B1) was incubated with serum collected from vaccinated animals at indicated time points and dilutions on ice for 60 min.

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: .. Flow cytometry-based ACE2 receptor binding inhibition assay HEK-293T cells stably expressing ACE2-GFP were generated using retroviral transduction. .. Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells.

    Cytometry:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: .. Flow cytometry-based ACE2 receptor binding inhibition assay HEK-293T cells stably expressing ACE2-GFP were generated using retroviral transduction. .. Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells.

    Binding Assay:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: .. Flow cytometry-based ACE2 receptor binding inhibition assay HEK-293T cells stably expressing ACE2-GFP were generated using retroviral transduction. .. Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells.

    Inhibition:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: .. Flow cytometry-based ACE2 receptor binding inhibition assay HEK-293T cells stably expressing ACE2-GFP were generated using retroviral transduction. .. Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells.

    Stable Transfection:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: .. Flow cytometry-based ACE2 receptor binding inhibition assay HEK-293T cells stably expressing ACE2-GFP were generated using retroviral transduction. .. Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells.

    Generated:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: .. Flow cytometry-based ACE2 receptor binding inhibition assay HEK-293T cells stably expressing ACE2-GFP were generated using retroviral transduction. .. Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells.

    Transduction:

    Article Title: Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    Article Snippet: .. Flow cytometry-based ACE2 receptor binding inhibition assay HEK-293T cells stably expressing ACE2-GFP were generated using retroviral transduction. .. Following transduction, the cells were flow sorted based on GFP expression to isolate GFP positive cells.

    Synthesized:

    Article Title: Virus-free and live-cell visualizing SARS-CoV-2 cell entry for studies of neutralizing antibodies and compound inhibitors
    Article Snippet: For lentiviral vectors, the pLVEF1αIHRB-ACE2hR and pLVEF1αmNG vectors were constructed on the pLV-EF1α-MCS-IRES-Bsd vector (Youbio, VT8179). .. The ACE2 cDNA fragment (Sino Biological, HG10108-ACG) linking with an IRES-H2BmRuby3-P2A-BsR DNA fragment (synthesized by Generalbiol, Anhui, China) was cloned in-frame into the XbaI/SalI sites of pLV-EF1α-MCS-IRES-Bsd to obtain the pLVEF1αIHRB-ACE2hR vector. .. Recombinant proteinsRecombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133).

    Plasmid Preparation:

    Article Title: Virus-free and live-cell visualizing SARS-CoV-2 cell entry for studies of neutralizing antibodies and compound inhibitors
    Article Snippet: For lentiviral vectors, the pLVEF1αIHRB-ACE2hR and pLVEF1αmNG vectors were constructed on the pLV-EF1α-MCS-IRES-Bsd vector (Youbio, VT8179). .. The ACE2 cDNA fragment (Sino Biological, HG10108-ACG) linking with an IRES-H2BmRuby3-P2A-BsR DNA fragment (synthesized by Generalbiol, Anhui, China) was cloned in-frame into the XbaI/SalI sites of pLV-EF1α-MCS-IRES-Bsd to obtain the pLVEF1αIHRB-ACE2hR vector. .. Recombinant proteinsRecombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133).

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  • 94
    Sino Biological ace2 gfp
    Ace2 Gfp, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ace2 gfp/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ace2 gfp - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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