Journal: Cell Genomics

Article Title: Single-cell transcriptomics provides insights into the stress-immune interplay and informs disease risk

doi: 10.1016/j.xgen.2026.101330

Figure Lengend Snippet:

Article Snippet: In situ hybridization was performed using ACD Bio’s RNAScope technology per the kit instructions (Multiplex Fluorescent Detection Kit, ACD Bio, #323110).

Techniques: Recombinant, Electron Microscopy, Selection, cDNA Synthesis, RNAscope, Multiplex Assay, Single Cell, Software

Journal: Nature Communications

Article Title: Cellular and subcellular heterogeneity of astrocytic Na⁺ homeostasis tuning astrocytes into functionally distinct subgroups in the mouse brain

doi: 10.1038/s41467-026-73435-z

Figure Lengend Snippet: a RNAscope for ATP1b1 and ATP1b2. Left, upper and middle panel: Double-labeling of mRNA for subunits NKA β1 or β2 with immunohistochemical labeling for GFAP and merge of the two channels. Left, lower panel: Same tissue section, showing double-labeling of mRNA for β1 and β2 and merge. Right: Composite image of tissue section on the left, showing labeling for ATP1b1, ATP1b2, GFAP and DAPI. Please note that in all stainings, only a small proportion of cells are positive for one of the NKA subunit markers, while the majority of labeled cells are positive for both. Similar results were independently obtained at least 3 times for each experimental condition. b Subsequential immunohistochemical double-labeling for the NKA subunits β1 and β2. Note the different levels of perineuronal/neuronal labeling of both subunits in the stratum pyramidale . Similar results were independently obtained at least 3 times for each experimental condition. a , b single-colored arrowheads point out cells exhibiting only one label, double-colored arrowheads show cells labeled by both labels used. StrP: stratum pyramidale , StrR: stratum radiatum . a , b All scale bars: 80 µm.

Article Snippet: To quantify mRNA expression, Advanced Cell Diagnostics (ACD) RNAscope® Multiplex Fluorescent V2 Assay was performed in combination with immunohistochemistry (IHC) according to the manufacturers protocol .

Techniques: RNAscope, Labeling, Immunohistochemical staining