accuprime taq dna polymerase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    AccuPrime Taq DNA Polymerase System
    Description:
    The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody mediated hot start protocol for improved PCR specificity over other hot start DNA polymerases see figure Platinum anti Taq DNA polymerase antibodies inhibit polymerase activity providing an automatic hot start while a thermostable accessory protein enhances specific primer template hybridization during every cycle of PCR This combination improves the fidelity of Taq DNA Polymerase by two fold and is ideal for high throughput screening and multiplex PCR AccuPrime Taq DNA Polymerase broadens primer annealing temperatures giving you optimal performance between 55°C and 65°C Advantages of AccuPrime Taq DNA Polymerase • Specificity control mispriming at every cycle to get single band PCR products• Ease of use minimize reaction optimization and primer set redesign• Convenience conveniently assemble reactions at room temperatureAccuPrime buffersThe 10X AccuPrime buffers contain thermostable AccuPrime protein Mg and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR Two individual buffer systems 10X AccuPrime PCR Buffer I and II are provided for amplification of specific types of templates 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons 200bp cDNA or plasmids 10X AccuPrime PCR Buffer II is designed for genomic DNA 200 bp 4 kb ApplicationsAccuPrime Taq DNA Polymerase can be used for a wide variety of applications including multiplex PCR TOPO TA Cloning and allele specific amplifications
    Catalog Number:
    12339016
    Price:
    None
    Applications:
    Amplification of Bisulfite-Treated DNA|Hot Start PCR|Multiplex PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes|RNAi, Epigenetics & Non-Coding RNA Research|Routine PCR|Methylation Analysis
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher accuprime taq dna polymerase
    The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody mediated hot start protocol for improved PCR specificity over other hot start DNA polymerases see figure Platinum anti Taq DNA polymerase antibodies inhibit polymerase activity providing an automatic hot start while a thermostable accessory protein enhances specific primer template hybridization during every cycle of PCR This combination improves the fidelity of Taq DNA Polymerase by two fold and is ideal for high throughput screening and multiplex PCR AccuPrime Taq DNA Polymerase broadens primer annealing temperatures giving you optimal performance between 55°C and 65°C Advantages of AccuPrime Taq DNA Polymerase • Specificity control mispriming at every cycle to get single band PCR products• Ease of use minimize reaction optimization and primer set redesign• Convenience conveniently assemble reactions at room temperatureAccuPrime buffersThe 10X AccuPrime buffers contain thermostable AccuPrime protein Mg and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR Two individual buffer systems 10X AccuPrime PCR Buffer I and II are provided for amplification of specific types of templates 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons 200bp cDNA or plasmids 10X AccuPrime PCR Buffer II is designed for genomic DNA 200 bp 4 kb ApplicationsAccuPrime Taq DNA Polymerase can be used for a wide variety of applications including multiplex PCR TOPO TA Cloning and allele specific amplifications
    https://www.bioz.com/result/accuprime taq dna polymerase/product/Thermo Fisher
    Average 97 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    accuprime taq dna polymerase - by Bioz Stars, 2020-08
    97/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Crosstalk between desmoglein-2/desmocollin-2/Src kinase and coxsackie and adenovirus receptor/ZO-1 protein complexes regulates blood-testis barrier dynamics
    Article Snippet: .. In short, a cDNA fragment of rat desmoglein-2 was amplified by PCR with specific primers containing cloning sites ( ) using AccuPrime™ Taq DNA polymerase (Invitrogen) and was cloned in-frame into the pET-41 Ek/LIC Vector (Novagen). .. Recombinant desmoglein-2 protein containing an N-terminal His6 tag was expressed in E. coli BL21 (DE3) cells after induction with IPTG and was purified with a nickel-chelated column (Pierce).

    Amplification:

    Article Title: Crosstalk between desmoglein-2/desmocollin-2/Src kinase and coxsackie and adenovirus receptor/ZO-1 protein complexes regulates blood-testis barrier dynamics
    Article Snippet: .. In short, a cDNA fragment of rat desmoglein-2 was amplified by PCR with specific primers containing cloning sites ( ) using AccuPrime™ Taq DNA polymerase (Invitrogen) and was cloned in-frame into the pET-41 Ek/LIC Vector (Novagen). .. Recombinant desmoglein-2 protein containing an N-terminal His6 tag was expressed in E. coli BL21 (DE3) cells after induction with IPTG and was purified with a nickel-chelated column (Pierce).

    Article Title: Characterization and tissue-specific expression patterns of the Plasmodium chabaudi cir multigene family
    Article Snippet: .. The amplification of these sequences was performed with the AccuPrime™ Taq DNA Polymerase (Invitrogen) with an initial denaturation for 30 s at 94°C, 40 cycles of 10 s at 94°C, 1 min at 45°C and 2 min at 72°C followed by a final extension for 10 min at 72°C. .. The PCR products of cDNA and genomic DNA were cloned, sequenced and aligned for verification of complete exon-intron structure of the cir genes.

    Article Title: CAG Repeat Variants in the POLG1 Gene Encoding mtDNA Polymerase-Gamma and Risk of Breast Cancer in African-American Women
    Article Snippet: .. The amplification was carried out in a 25 ul reaction containing approximately 50 ng of template DNA, 0.6 uM of both forward and reverse primers, and 0.5 ul AccuPrime™ Taq DNA polymerase (Invitrogen, USA) using a TECHNE TC-412 (96×0.2 ml) thermocycler (MIDSCI, USA). .. The PCR conditions were as follows: initial denaturation at 94°C for 4 min in 26 amplification cycles at 94°C for 10 sec, annealing at 61°C for 30 sec and 68°C for 40 sec, followed by a final extension step at 68°C for 5 min.

    Article Title: High throughput, high resolution selection of polymorphic microsatellite loci for multiplex analysis
    Article Snippet: .. PCR amplification of pooled DNA utilising unlabelled primer pairs specific to single microsatellite loci [ ] were performed incorporating 5 ng template DNA with AccuPrime Taq DNA Polymerase in supermix I, using half recommended volumes (Invitrogen Ltd). .. The thermal cycling protocol was 96°C for 2 min; 35 cycles of 96°C for 30 s, 51°C or 46°C for 30 s dependent on primer annealing characteristics, 72°C for 2 min; followed by 72°C for 10 min, in a MJ Research PTC-100 thermal cycler (Genetic Research Instrumentation Ltd).

    Irradiation:

    Article Title: New York City House Mice ( Mus musculus) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
    Article Snippet: .. PCR master mix was prepared by adding 11.35 µl DNA-free PCR water (Qiagen), 2 µl PCR buffer II (Thermo Fisher Scientific), 0.15 µl (0.75 U) of AccuPrime Taq DNA polymerase (Thermo Fisher Scientific), and 0.5 µl Sau3AI (2.5 U) (New England Biolabs, Ipswich, MA) and then UV irradiated and aliquoted into 96-well PCR plates. .. Primers that include unique 12-nt barcode sequences ( ) were added to each well prior to incubation at 37°C for 30 min to allow Sau3AI to digest contaminating double-stranded DNA (dsDNA).

    Polymerase Chain Reaction:

    Article Title: Crosstalk between desmoglein-2/desmocollin-2/Src kinase and coxsackie and adenovirus receptor/ZO-1 protein complexes regulates blood-testis barrier dynamics
    Article Snippet: .. In short, a cDNA fragment of rat desmoglein-2 was amplified by PCR with specific primers containing cloning sites ( ) using AccuPrime™ Taq DNA polymerase (Invitrogen) and was cloned in-frame into the pET-41 Ek/LIC Vector (Novagen). .. Recombinant desmoglein-2 protein containing an N-terminal His6 tag was expressed in E. coli BL21 (DE3) cells after induction with IPTG and was purified with a nickel-chelated column (Pierce).

    Article Title: The MOV10 Helicase Inhibits LINE-1 Mobility
    Article Snippet: .. The PCR was performed with the AccuPrime Taq DNA polymerase (Invitrogen) for 25–38 cycles under the following conditions: 94 °C for 50 s, 60 °C for 50 s, and 68 °C for 1 min. .. The DNA products were separated by electrophoresis in 1% agarose gels and visualized with ethidium bromide staining.

    Article Title: High throughput, high resolution selection of polymorphic microsatellite loci for multiplex analysis
    Article Snippet: .. PCR amplification of pooled DNA utilising unlabelled primer pairs specific to single microsatellite loci [ ] were performed incorporating 5 ng template DNA with AccuPrime Taq DNA Polymerase in supermix I, using half recommended volumes (Invitrogen Ltd). .. The thermal cycling protocol was 96°C for 2 min; 35 cycles of 96°C for 30 s, 51°C or 46°C for 30 s dependent on primer annealing characteristics, 72°C for 2 min; followed by 72°C for 10 min, in a MJ Research PTC-100 thermal cycler (Genetic Research Instrumentation Ltd).

    Article Title: Replication Fork Stability Is Essential for the Maintenance of Centromere Integrity in the Absence of Heterochromatin
    Article Snippet: .. All PCR was performed using Accuprime System (Invitrogen, 12339-016) using the primers described in . .. The PCR products were separated by 1× TAE, 2% agarose.

    Article Title: New York City House Mice ( Mus musculus) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants
    Article Snippet: .. PCR master mix was prepared by adding 11.35 µl DNA-free PCR water (Qiagen), 2 µl PCR buffer II (Thermo Fisher Scientific), 0.15 µl (0.75 U) of AccuPrime Taq DNA polymerase (Thermo Fisher Scientific), and 0.5 µl Sau3AI (2.5 U) (New England Biolabs, Ipswich, MA) and then UV irradiated and aliquoted into 96-well PCR plates. .. Primers that include unique 12-nt barcode sequences ( ) were added to each well prior to incubation at 37°C for 30 min to allow Sau3AI to digest contaminating double-stranded DNA (dsDNA).

    Positron Emission Tomography:

    Article Title: Crosstalk between desmoglein-2/desmocollin-2/Src kinase and coxsackie and adenovirus receptor/ZO-1 protein complexes regulates blood-testis barrier dynamics
    Article Snippet: .. In short, a cDNA fragment of rat desmoglein-2 was amplified by PCR with specific primers containing cloning sites ( ) using AccuPrime™ Taq DNA polymerase (Invitrogen) and was cloned in-frame into the pET-41 Ek/LIC Vector (Novagen). .. Recombinant desmoglein-2 protein containing an N-terminal His6 tag was expressed in E. coli BL21 (DE3) cells after induction with IPTG and was purified with a nickel-chelated column (Pierce).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Akt-Induced Phosphorylation of N-CoR at Serine 1450 Contributes to Its Misfolded Conformational Dependent Loss (MCDL) in Acute Myeloid Leukemia of the M5 Subtype
    Article Snippet: .. Converted cDNA was subjected to RT-PCR analysis using Accuprime Taq polymerase system (Invitrogen, Carlsbad, CA, USA). .. Dual Luciferase Reporter Assay 293T was co-transfected with 1 µg of full-length promoter/firefly luciferase reporter plasmid or promoter-less pGL3-basic vector, 5 ng of CMV/renilla luciferase plasmid and various dosages of pAct-Flag/N-CoR (WT) or S1450E, using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).

    Plasmid Preparation:

    Article Title: Crosstalk between desmoglein-2/desmocollin-2/Src kinase and coxsackie and adenovirus receptor/ZO-1 protein complexes regulates blood-testis barrier dynamics
    Article Snippet: .. In short, a cDNA fragment of rat desmoglein-2 was amplified by PCR with specific primers containing cloning sites ( ) using AccuPrime™ Taq DNA polymerase (Invitrogen) and was cloned in-frame into the pET-41 Ek/LIC Vector (Novagen). .. Recombinant desmoglein-2 protein containing an N-terminal His6 tag was expressed in E. coli BL21 (DE3) cells after induction with IPTG and was purified with a nickel-chelated column (Pierce).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher accuprime taq dna polymerase high fidelity
    Accuprime Taq Dna Polymerase High Fidelity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accuprime taq dna polymerase high fidelity/product/Thermo Fisher
    Average 99 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    accuprime taq dna polymerase high fidelity - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher accuprime taq dna polymerase
    Accuprime Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accuprime taq dna polymerase/product/Thermo Fisher
    Average 97 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    accuprime taq dna polymerase - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

    Image Search Results