accuprime pfx dna polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher accuprime pfx dna polymerase
    Accuprime Pfx Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accuprime pfx dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    accuprime pfx dna polymerase - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: .. Cloning and mutagenesis ATX3‐WT, C14A and UIM* variants cDNAs were amplified by AccuPrime Pfx DNA polymerase (Invitrogen) using Veriti 96‐well thermal cycler (Applied Biosystems). .. Plasmids pFLAG‐6A‐ATX3Q_22 (WT), C14A and UIM* from Addgene were used as templates to amplify the cDNAs.

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: Paragraph title: Cloning and mutagenesis ... ATX3 variants were generated by site‐directed mutagenesis using AccuPrime Pfx DNA polymerase (Invitrogen) according to the manufacturer's protocol and confirmed by DNA sequencing (Source Biosciences).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: PCR amplifications were performed using AccuPrime Pfx DNA polymerase (Thermo Fisher Scientific). .. PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations.

    Article Title: The olfactory secretome varies according to season in female sheep and goat
    Article Snippet: PCR products were cloned into pCR4®-TOPO vector (TOPO™-TA cloning™ kit, Invitrogen), then amplified into Escherichia coli One Shot™ Top10 chemically competent cells (Invitrogen). .. PCR amplification was performed from 5 μL of RT template with AccuPrime™ Pfx DNA Polymerase and Platinum™ Taq DNA Polymerase High Fidelity at a Tm of 65 °C, according to manufacturer’s protocol (Invitrogen).

    Article Title: CpG DNA activation and plasma-cell differentiation of CD27− naive human B cells
    Article Snippet: Rearranged VH3 gene segments were amplified from approximately 2000 cells with AccuPrime Pfx DNA polymerase (Invitrogen). .. Gel-purified polymerase chain reaction (PCR) products were cloned using the Zero Blunt TOPO PCR Cloning Kit (Invitrogen), and positive colonies were sequenced.

    Amplification:

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA). .. MG191 and MG 309 loci were amplified using MgPa-1/MgPa-3 and MG309-STR-F/MG309-STR-R primer sets, respectively.

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: .. Cloning and mutagenesis ATX3‐WT, C14A and UIM* variants cDNAs were amplified by AccuPrime Pfx DNA polymerase (Invitrogen) using Veriti 96‐well thermal cycler (Applied Biosystems). .. Plasmids pFLAG‐6A‐ATX3Q_22 (WT), C14A and UIM* from Addgene were used as templates to amplify the cDNAs.

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: Cloning and mutagenesis ATX3‐WT, C14A and UIM* variants cDNAs were amplified by AccuPrime Pfx DNA polymerase (Invitrogen) using Veriti 96‐well thermal cycler (Applied Biosystems). .. ATX3 variants were generated by site‐directed mutagenesis using AccuPrime Pfx DNA polymerase (Invitrogen) according to the manufacturer's protocol and confirmed by DNA sequencing (Source Biosciences).

    Article Title: The olfactory secretome varies according to season in female sheep and goat
    Article Snippet: .. PCR amplification was performed from 5 μL of RT template with AccuPrime™ Pfx DNA Polymerase and Platinum™ Taq DNA Polymerase High Fidelity at a Tm of 65 °C, according to manufacturer’s protocol (Invitrogen). .. PCR products were cloned either into pCR®-Blunt II-TOPO or into pCR4®-TOPO vectors, respectively (Invitrogen).

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA). .. MG191 and MG 309 loci were amplified using MgPa-1/MgPa-3 and MG309-STR-F/MG309-STR-R primer sets, respectively.

    Article Title: CpG DNA activation and plasma-cell differentiation of CD27− naive human B cells
    Article Snippet: .. Rearranged VH3 gene segments were amplified from approximately 2000 cells with AccuPrime Pfx DNA polymerase (Invitrogen). .. For the amplification, a VH3 primer mix (5′-GAGGTGCAGCTGGTGGAGTCTGG-3′ and 5′-GAGGTGCAGCTGTTGGAGTCTGG-3′ at a 7:3 ratio) and a JH primer mix were used (30 seconds at 95°C and 60 seconds at 68°C for 35 cycles).

    Agarose Gel Electrophoresis:

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: Nested PCR was performed if first amplicons were not detected by agarose gel. .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA).

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: Nested PCR was performed if first amplicons were not detected by agarose gel. .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA).

    Ligation:

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: To exclude 2S rRNA from sequencing libraries, 10 pmol 2S blocker oligo was added before 5′ adaptor ligation ( ). .. 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024).

    Mutagenesis:

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: Amplicons (wild type or mutant) were sequenced using the same PCR primers to verify mutations. .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA).

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: .. Cloning and mutagenesis ATX3‐WT, C14A and UIM* variants cDNAs were amplified by AccuPrime Pfx DNA polymerase (Invitrogen) using Veriti 96‐well thermal cycler (Applied Biosystems). .. Plasmids pFLAG‐6A‐ATX3Q_22 (WT), C14A and UIM* from Addgene were used as templates to amplify the cDNAs.

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: .. ATX3 variants were generated by site‐directed mutagenesis using AccuPrime Pfx DNA polymerase (Invitrogen) according to the manufacturer's protocol and confirmed by DNA sequencing (Source Biosciences). ..

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: 3′ adaptor was ligated using truncated, K227Q mutant T4 RNA Ligase 2 (made in lab) at 25°C for ≥16 h, sRNAs precipitated, and size selected as described in ( ). .. 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024).

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: Amplicons (wild type or mutant) were sequenced using the same PCR primers to verify mutations. .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA).

    Isolation:

    Article Title: Yeast Display of Engineered Antibody Domains
    Article Snippet: AccuPrime™ Pfx DNA Polymerase (Invitrogen). .. Plasmid isolation kit (Qiagen).

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: In summary, total RNA (50 μg) was extracted using mirVana miRNA Isolation Kit (Life Technologies, #AM1561) and purified by 15% urea polyacrylamide gel electrophoresis (PAGE), selecting for 18–30 nt small RNAs. .. 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024).

    RNA Sequencing Assay:

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024). .. Small RNA-seq libraries for three independent biological replicates were sequenced using a NextSeq500 (Illumina) to obtain 75 nt single-end reads. sRNA-seq analysis was performed with piPipes using default options (v1.4; ).

    Purification:

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: Half of the purified sRNAs were oxidized with NaIO4 was used to deplete miRNAs and enrich for siRNAs and piRNAs ( ). .. 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: PCR amplifications were performed using AccuPrime Pfx DNA polymerase (Thermo Fisher Scientific). .. PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations.

    Article Title: The olfactory secretome varies according to season in female sheep and goat
    Article Snippet: Recombinant plasmids were purified with QIAprep Spin Miniprep kit (Qiagen) and sequenced in both senses (Eurofins Genomics). .. PCR amplification was performed from 5 μL of RT template with AccuPrime™ Pfx DNA Polymerase and Platinum™ Taq DNA Polymerase High Fidelity at a Tm of 65 °C, according to manufacturer’s protocol (Invitrogen).

    Plasmid Preparation:

    Article Title: Yeast Display of Engineered Antibody Domains
    Article Snippet: Paragraph title: 2.2. Preparation of DNA Inserts and Vector for Library Construction ... AccuPrime™ Pfx DNA Polymerase (Invitrogen).

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: Cloning and mutagenesis ATX3‐WT, C14A and UIM* variants cDNAs were amplified by AccuPrime Pfx DNA polymerase (Invitrogen) using Veriti 96‐well thermal cycler (Applied Biosystems). .. The cDNAs were cloned in pCDNA5‐FRT/TO‐nEGFP vector using BamH1 and Xho1 restriction endonucleases (NEW ENGLAND BioLabs) and confirmed by DNA sequencing (Source Biosciences).

    Article Title: The olfactory secretome varies according to season in female sheep and goat
    Article Snippet: PCR products were cloned into pCR4®-TOPO vector (TOPO™-TA cloning™ kit, Invitrogen), then amplified into Escherichia coli One Shot™ Top10 chemically competent cells (Invitrogen). .. PCR amplification was performed from 5 μL of RT template with AccuPrime™ Pfx DNA Polymerase and Platinum™ Taq DNA Polymerase High Fidelity at a Tm of 65 °C, according to manufacturer’s protocol (Invitrogen).

    Nested PCR:

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: Nested PCR was performed if first amplicons were not detected by agarose gel. .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA).

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: Nested PCR was performed if first amplicons were not detected by agarose gel. .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA).

    Generated:

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: Cloning and mutagenesis ATX3‐WT, C14A and UIM* variants cDNAs were amplified by AccuPrime Pfx DNA polymerase (Invitrogen) using Veriti 96‐well thermal cycler (Applied Biosystems). .. ATX3 variants were generated by site‐directed mutagenesis using AccuPrime Pfx DNA polymerase (Invitrogen) according to the manufacturer's protocol and confirmed by DNA sequencing (Source Biosciences).

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: .. ATX3 variants were generated by site‐directed mutagenesis using AccuPrime Pfx DNA polymerase (Invitrogen) according to the manufacturer's protocol and confirmed by DNA sequencing (Source Biosciences). ..

    Polymerase Chain Reaction:

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA). ..

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: .. 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024). .. Small RNA-seq libraries for three independent biological replicates were sequenced using a NextSeq500 (Illumina) to obtain 75 nt single-end reads. sRNA-seq analysis was performed with piPipes using default options (v1.4; ).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: .. PCR amplifications were performed using AccuPrime Pfx DNA polymerase (Thermo Fisher Scientific). ..

    Article Title: The olfactory secretome varies according to season in female sheep and goat
    Article Snippet: .. PCR amplification was performed from 5 μL of RT template with AccuPrime™ Pfx DNA Polymerase and Platinum™ Taq DNA Polymerase High Fidelity at a Tm of 65 °C, according to manufacturer’s protocol (Invitrogen). .. PCR products were cloned either into pCR®-Blunt II-TOPO or into pCR4®-TOPO vectors, respectively (Invitrogen).

    Article Title: Mycoplasma genitalium Infections with Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
    Article Snippet: .. PCR was carried out with a 25 μL PCR reaction volume containing 0.4 μmol/L of each primer, 2.5 μL of 10X AccuPrime Pfx reaction mix and 0.5 U of AccuPrime Pfx DNA polymerase (Thermo Fisher, CA). ..

    Article Title: High throughput, efficacious gene editing genome surveillance in Chinese hamster ovary cells
    Article Snippet: .. PCR PCR reactions in this study utilized AccuPrime Pfx DNA Polymerase (Invitrogen, Cat: 12344024) and an ABI Veriti thermocycler. ..

    Article Title: Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
    Article Snippet: .. Alternatively, the PCR can be performed using AccuPrime Pfx DNA Polymerase: 5 μl 10× AccuPrime Pfx Reaction Mix (Invitrogen) 1 μl 10 μM PCR Primer 2 (one of the twelve to provide the barcode) 1 μl AccuPrime Pfx DNA Polymerase (2.5 U/μl) 11 μl water. .. After the incubation, pause the PCR machine, and then add 1 μl 10 μM PCR Primer 1.

    Article Title: CpG DNA activation and plasma-cell differentiation of CD27− naive human B cells
    Article Snippet: Rearranged VH3 gene segments were amplified from approximately 2000 cells with AccuPrime Pfx DNA polymerase (Invitrogen). .. Gel-purified polymerase chain reaction (PCR) products were cloned using the Zero Blunt TOPO PCR Cloning Kit (Invitrogen), and positive colonies were sequenced.

    DNA Sequencing:

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: Cloning and mutagenesis ATX3‐WT, C14A and UIM* variants cDNAs were amplified by AccuPrime Pfx DNA polymerase (Invitrogen) using Veriti 96‐well thermal cycler (Applied Biosystems). .. The cDNAs were cloned in pCDNA5‐FRT/TO‐nEGFP vector using BamH1 and Xho1 restriction endonucleases (NEW ENGLAND BioLabs) and confirmed by DNA sequencing (Source Biosciences).

    Article Title: The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8
    Article Snippet: .. ATX3 variants were generated by site‐directed mutagenesis using AccuPrime Pfx DNA polymerase (Invitrogen) according to the manufacturer's protocol and confirmed by DNA sequencing (Source Biosciences). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: In summary, total RNA (50 μg) was extracted using mirVana miRNA Isolation Kit (Life Technologies, #AM1561) and purified by 15% urea polyacrylamide gel electrophoresis (PAGE), selecting for 18–30 nt small RNAs. .. 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024).

    Touchdown PCR:

    Article Title: The olfactory secretome varies according to season in female sheep and goat
    Article Snippet: Amplifications were performed from 5 μL of RT template using Platinum™ Taq DNA Polymerase High Fidelity (Invitrogen) by touchdown PCR as follow: 94 °C for 2 min, followed by 5 cycles of 15 s at 94 °C and 30 s at 72 °C; 5 cycles of 15 s at 94 °C and 30 s at 70 °C; 25 cycles of 15 s at 94 °C, 30 s at 65 °C and 1 min at 68 °C. .. PCR amplification was performed from 5 μL of RT template with AccuPrime™ Pfx DNA Polymerase and Platinum™ Taq DNA Polymerase High Fidelity at a Tm of 65 °C, according to manufacturer’s protocol (Invitrogen).

    Sequencing:

    Article Title: Maelstrom Represses Canonical Polymerase II Transcription within Bi-Directional piRNA Clusters in Drosophila melanogaster
    Article Snippet: To exclude 2S rRNA from sequencing libraries, 10 pmol 2S blocker oligo was added before 5′ adaptor ligation ( ). .. 5′ adaptor was added using T4 RNA ligase (Life Technologies, #AM2141) at 25°C for 2.5 h, followed by reverse-transcription using AMV reverse transcriptase (New England Biolabs, #M0277L) and PCR using AccuPrime Pfx DNA Polymerase (Invitrogen, #12344-024).

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: Paragraph title: RNA editing analysis by Sanger sequencing ... PCR amplifications were performed using AccuPrime Pfx DNA polymerase (Thermo Fisher Scientific).

    Article Title: CpG DNA activation and plasma-cell differentiation of CD27− naive human B cells
    Article Snippet: Paragraph title: Germ-line sequence examination of CD138+ cells ... Rearranged VH3 gene segments were amplified from approximately 2000 cells with AccuPrime Pfx DNA polymerase (Invitrogen).

    Gel Extraction:

    Article Title: Apobec1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver
    Article Snippet: PCR amplifications were performed using AccuPrime Pfx DNA polymerase (Thermo Fisher Scientific). .. PCR products were then gel purified using Qiaquick Gel Extraction kit (Qiagen) and cloned using Zero Blunt TOPO PCR Cloning kit (Invitrogen) following manufacturer's recommendations.

    Recombinant:

    Article Title: The olfactory secretome varies according to season in female sheep and goat
    Article Snippet: Recombinant plasmids were purified with QIAprep Spin Miniprep kit (Qiagen) and sequenced in both senses (Eurofins Genomics). .. PCR amplification was performed from 5 μL of RT template with AccuPrime™ Pfx DNA Polymerase and Platinum™ Taq DNA Polymerase High Fidelity at a Tm of 65 °C, according to manufacturer’s protocol (Invitrogen).

    Derivative Assay:

    Article Title: CpG DNA activation and plasma-cell differentiation of CD27− naive human B cells
    Article Snippet: Magnetic bead immunoaffinity columns were used to remove dead cells and enrich CD138+ cells following phase III of the culture system for cells derived from CD27+ and CD27− precursors. .. Rearranged VH3 gene segments were amplified from approximately 2000 cells with AccuPrime Pfx DNA polymerase (Invitrogen).

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  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher accuprime pfx dna polymerase
    Accuprime Pfx Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accuprime pfx dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 391 article reviews
    Price from $9.99 to $1999.99
    accuprime pfx dna polymerase - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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