acclaim pepmap100 c18  (Thermo Fisher)


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    Name:
    Acclaim PepMap 100 C18 LC Columns
    Description:
    Generate high resolution analyses of tryptic natural and synthetic peptides using Thermo Scientific Acclaim PepMap 100 C18 LC Columns These columns are often used for LC MS MS peptide mapping for protein identification biomarker discovery and systems biology Due to their high loading capacity Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples Most of these C18 columns are pre assembled with Thermo Scientific nanoViper Fingertight Fittings for easy installation Classic columns are available as well
    Catalog Number:
    160321
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Chromatography Columns Resins Spin Filters
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    Structured Review

    Thermo Fisher acclaim pepmap100 c18
    Generate high resolution analyses of tryptic natural and synthetic peptides using Thermo Scientific Acclaim PepMap 100 C18 LC Columns These columns are often used for LC MS MS peptide mapping for protein identification biomarker discovery and systems biology Due to their high loading capacity Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples Most of these C18 columns are pre assembled with Thermo Scientific nanoViper Fingertight Fittings for easy installation Classic columns are available as well
    https://www.bioz.com/result/acclaim pepmap100 c18/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acclaim pepmap100 c18 - by Bioz Stars, 2020-02
    90/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes
    Article Snippet: TiO2 beads were packed by centrifugation in empty spin columns (Gygen Corporation; Cat. TT2EMT) previously equilibrated using glycolic acid, 50% ACN and ammonium bicarbonate buffer (20 mM NH4 HCO3 (pH: 6.8) in 50% ACN). .. Phosphopeptides were loaded in a μ-pre-column (Acclaim™ PepMap™ 100 C18 LC; Cat 160454, Thermo Fisher Scientific, Waltham, MA, USA) and separated in an analytical column (Acclaim™ PepMap™ 100 C18 LC; Cat. 164569, Thermo Fisher Scientific, Waltham, MA, USA) using a gradient that runs from 3% to 23% B over 120 min.

    Injection:

    Article Title: A cytoplasmic COMPASS is necessary for cell survival and triple-negative breast cancer pathogenesis by regulating metabolism
    Article Snippet: Three micrograms of each fraction was loaded onto an autosampler with a Thermo Easy nLC 1000 UPLC pump onto a vented Acclaim Pepmap 100 (75 µm × 2 cm) nanoViper trap column coupled to a 3-µm nanoViper analytical column at 100 Å, C18, 0.075 mm, and 500 mm (Thermo, 164570) with a stainless steel emitter tip assembled on the Nanospray Flex ion source with a spray voltage of 2000 V. Buffer A contained 94.785% H2 O with 5% ACN and 0.125% FA, and buffer B contained 99.875% ACN with 0.125% FA. .. Detector type was set to Orbitrap, with 60-K resolution, wide quad isolation, mass range normal, scan range 300–1500 (m/z), maximum injection time 50 msec, AGC target 200,000, 1 microscan, S-lens RF level 60, without source fragmentation, and centroid data type.

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: The isolation window was set to 1.6 m/z and the AGC target to 1.0e4 with maximum injection time of 100 ms. Ions were injected for all available parallelizable time. .. Samples for HCT116 PL1 labeling were analyzed on a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A ) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Q Exactive Plus mass spectrometer (Thermo Fisher Scientific).

    Article Title: DYRK1A Overexpression Alters Cognition and Neural-Related Proteomic Pathways in the Hippocampus That Are Rescued by Green Tea Extract and/or Environmental Enrichment
    Article Snippet: Liquid Chromatography-Tandem Mass Spectrometry For each sample, 1 μg of tryptic peptides from digested hippocampal tissue and phospho-enriched peptides from 100 μg of the same tissue were injected in an LTQ-Orbitrap Velos Pro mass spectrometer (Thermo Fisher Scientific) coupled to a nano-LC (EASY-nLC, Proxeon). .. Nano-LC was equipped with a reversed-phase chromatography column of 25 cm with an inner diameter of 75 μm, packed with 3 μm C18 particles (Nikkyo Technos, NTCC-360/75-3-25L), and a Nano Trap Column Acclaim PepMap100 100 μm × 2 cm C18, 5 μm, 100 A (Thermo, 164199).

    Article Title: C7orf59/LAMTOR4 phosphorylation and structural flexibility modulate Ragulator assembly
    Article Snippet: Peptides were separated running a linear gradient from 2 to 30% mobile phase B on a Acclaim™ PepMap™ 100 C18 LC Columns (Thermo Scientific), 3 µm, 150 mm length analytical column kept at 40 °C with 0.3 μL·min−1 flow over 120 min. MS data were analyzed in the Orbitrap in the range 400–2000 m/z at resolution of 120 000 (at m/z 400). .. The maximum injection time was set to 50 ms with automatic gain control (AGC) 106 .

    Article Title: Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44
    Article Snippet: The peptide samples were loaded on a nano viper trap column (C18, 5 µm, 100 Å, 100 µm × 2 cm; PN: 164562, Thermo Scientific) and resolved on a 50 cm analytical column (2 µm, 100 Å, 75 µm × 50 cm. .. Both the TMT-labeled phosphoproteome and total proteome fractions from PS1 and PS2 (phosphoproteome and total proteome) were injected as technical replicates.

    Mass Spectrometry:

    Article Title: The DUF1669 domain of FAM83 family proteins anchor Casein Kinase 1 isoforms
    Article Snippet: Paragraph title: Mass Spectrometry ... Peptides were trapped on a nanoViper Trap column, 2 cm x 100 μm C18 5 μm 100 Å (Thermo, 164564) then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: A cytoplasmic COMPASS is necessary for cell survival and triple-negative breast cancer pathogenesis by regulating metabolism
    Article Snippet: Paragraph title: Mass spectrometry (MS) ... Three micrograms of each fraction was loaded onto an autosampler with a Thermo Easy nLC 1000 UPLC pump onto a vented Acclaim Pepmap 100 (75 µm × 2 cm) nanoViper trap column coupled to a 3-µm nanoViper analytical column at 100 Å, C18, 0.075 mm, and 500 mm (Thermo, 164570) with a stainless steel emitter tip assembled on the Nanospray Flex ion source with a spray voltage of 2000 V. Buffer A contained 94.785% H2 O with 5% ACN and 0.125% FA, and buffer B contained 99.875% ACN with 0.125% FA.

    Article Title: Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes
    Article Snippet: Phosphopetide pellets were resuspended in 9 µL of reconstitution buffer (20 fmol/µL enolase in 3% ACN, 0.1% TFA) and 5.0 µL were loaded onto an LC-MS/MS system consisting of a Dionex UltiMate 3000 RSLC directly coupled to an Orbitrap Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Phosphopeptides were loaded in a μ-pre-column (Acclaim™ PepMap™ 100 C18 LC; Cat 160454, Thermo Fisher Scientific, Waltham, MA, USA) and separated in an analytical column (Acclaim™ PepMap™ 100 C18 LC; Cat. 164569, Thermo Fisher Scientific, Waltham, MA, USA) using a gradient that runs from 3% to 23% B over 120 min.

    Article Title: Mechanisms underpinning the permanent muscle damage induced by snake venom metalloprotease
    Article Snippet: Paragraph title: Mass spectrometry ... Samples were then trapped on an Acclaim PepMap 100 C18 LC column, 5um, 100A 300um i.d. x 5mm (Dionex, USA), then further separated in Nano Series Standard Columns 75μm i.d. x 15 cm.

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Paragraph title: Mass spectrometry ... Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
    Article Snippet: Paragraph title: Mass spectrometry ... Peptides were trapped on a nanoViper Trap column, 2 cm × 100 μm C18 5 μm 100 Å (Thermo, 164564), then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B [Solvent A: 2% Acetonitrile, 0.1% formic acid, 3% DMSO in H2 O; Solvent B: 80% acetonitrile, 0.08% formic acid, 3% DMSO in H2 O].

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: .. Samples for HCT116 PL1 labeling were analyzed on a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A ) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Q Exactive Plus mass spectrometer (Thermo Fisher Scientific). ..

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: .. LC-MS/MS Analysis of Proteomics Samples Samples were analyzed via LC-MS/MS using a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Thermo Fisher LTQ Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). .. Samples were loaded onto the trap column at a flow rate of 5 μL/min with aqueous 0.1% trifluoracetic acid (TFA) and then transferred onto the separation column at 0.3 μL/min.

    Article Title: DYRK1A Overexpression Alters Cognition and Neural-Related Proteomic Pathways in the Hippocampus That Are Rescued by Green Tea Extract and/or Environmental Enrichment
    Article Snippet: Paragraph title: Liquid Chromatography-Tandem Mass Spectrometry ... Nano-LC was equipped with a reversed-phase chromatography column of 25 cm with an inner diameter of 75 μm, packed with 3 μm C18 particles (Nikkyo Technos, NTCC-360/75-3-25L), and a Nano Trap Column Acclaim PepMap100 100 μm × 2 cm C18, 5 μm, 100 A (Thermo, 164199).

    Article Title: The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development
    Article Snippet: Paragraph title: Identification of proteins and phosphorylation sites by tandem mass spectrometry ... 1 to 6 μl of peptide containing sample solution were trapped and washed with 100% solvent A (98% water, 2% acetonitrile, 0.07% trifluoroacetic acid) on an Acclaim PepMap 100 pre-column (#164564, 100 μm x 2 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) with a flow rate of 25 μl/min for 6 min. An Acclaim PepMap RSLC column (#164540, 75 μm or 50 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) was used for analytical peptide separation by reverse phase chromatography.

    Article Title: Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia
    Article Snippet: Paragraph title: Mass spectrometry analysis ... Peptides were trapped on a NanoViper trap column, 2 cm × 100 mm C18 5 mm 100 Å (Thermo, 164564) and then separated on a 15-cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% solvent B [solvent A: 2% acetonitrile, 0.1% formic acid and 3% DMSO in H2 O; solvent B: 80% acetonitrile, 0.08% formic acid and 3% DMSO in H2 O].

    Article Title: C7orf59/LAMTOR4 phosphorylation and structural flexibility modulate Ragulator assembly
    Article Snippet: .. Peptides were separated running a linear gradient from 2 to 30% mobile phase B on a Acclaim™ PepMap™ 100 C18 LC Columns (Thermo Scientific), 3 µm, 150 mm length analytical column kept at 40 °C with 0.3 μL·min−1 flow over 120 min. MS data were analyzed in the Orbitrap in the range 400–2000 m/z at resolution of 120 000 (at m/z 400). ..

    Article Title: Corona Composition Can Affect the Mechanisms Cells Use to Internalize Nanoparticles
    Article Snippet: .. The supernatant containing the peptides was collected and dried using a speed vacuum for about 2 h. Afterward, samples were resuspended in 75 μL of 0.1% formic acid in water, and 2 μL samples were loaded into a Q Exactive Plus hybrid quadrupole–orbitrap mass spectrometer (ThermoFisher) using Acclaim PepMap 100 C18 LC columns (ThermoFisher). .. Samples were analyzed using the software PEAKS Studio 8.5 (Bioinformatics Solutions Inc.), and proteins identified using the manually reviewed UniProtKB/Swiss-Prot database.

    Article Title: Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44
    Article Snippet: LC–MS/MS analysis Both the PS1 and PS2 phopshoproteome and total proteome samples are analyzed on an Orbitrap Fusion Tribrid Mass spectrometer (Thermo Fisher Scientific, San Jose, U.S.A.) that was in line with the Dionex 3000 RSLC nano-liquid chromatography system, similar to previously published work [ – ]. .. The peptide samples were loaded on a nano viper trap column (C18, 5 µm, 100 Å, 100 µm × 2 cm; PN: 164562, Thermo Scientific) and resolved on a 50 cm analytical column (2 µm, 100 Å, 75 µm × 50 cm.

    Incubation:

    Article Title: Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes
    Article Snippet: For phosphopeptide elution, beads were incubated for 1 min at room temperature with 50 µL of 5% NH4 OH in 50% ACN and centrifuged. .. Phosphopeptides were loaded in a μ-pre-column (Acclaim™ PepMap™ 100 C18 LC; Cat 160454, Thermo Fisher Scientific, Waltham, MA, USA) and separated in an analytical column (Acclaim™ PepMap™ 100 C18 LC; Cat. 164569, Thermo Fisher Scientific, Waltham, MA, USA) using a gradient that runs from 3% to 23% B over 120 min.

    Article Title: Mechanisms underpinning the permanent muscle damage induced by snake venom metalloprotease
    Article Snippet: Trypsin gold (Promega, UK) was subsequently added and the samples were incubated overnight at 37°C. .. Samples were then trapped on an Acclaim PepMap 100 C18 LC column, 5um, 100A 300um i.d. x 5mm (Dionex, USA), then further separated in Nano Series Standard Columns 75μm i.d. x 15 cm.

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Mass spectrometry 10.5 mg of protein in soluble cell extract from HaCaT cells was pre-cleared by incubation with Protein G-Sepharose for 30 min at 4°C, then incubated with GFP-Trap beads (ChromoTek, gta-10) for 4 h at 4°C. .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia
    Article Snippet: Reactions were stopped at the time shown and incubated at 95°C in 1× LDS (Invitrogen) 5 mM DTT for 1 min. Alkylation occurred at room temperature for 30 min by the addition of 20 mM iodoacetamide (20 mM) to the samples. .. Peptides were trapped on a NanoViper trap column, 2 cm × 100 mm C18 5 mm 100 Å (Thermo, 164564) and then separated on a 15-cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% solvent B [solvent A: 2% acetonitrile, 0.1% formic acid and 3% DMSO in H2 O; solvent B: 80% acetonitrile, 0.08% formic acid and 3% DMSO in H2 O].

    Expressing:

    Article Title: The DUF1669 domain of FAM83 family proteins anchor Casein Kinase 1 isoforms
    Article Snippet: The expression of GFP-tagged FAM83 proteins in stable Flp-In T-Rex HEK 293 and U2OS cells was induced with 20 ng/ml doxycycline for 24 h prior to lysis. .. Peptides were trapped on a nanoViper Trap column, 2 cm x 100 μm C18 5 μm 100 Å (Thermo, 164564) then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    High Performance Liquid Chromatography:

    Article Title: The DUF1669 domain of FAM83 family proteins anchor Casein Kinase 1 isoforms
    Article Snippet: The peptides were subjected to mass spectrometric analysis performed by LC-MS-MS on a Linear ion trap-orbitrap hybrid mass spectrometer (Orbitrap-VelosPro, Thermo) coupled to a U3000 RSLC Hplc (Thermo). .. Peptides were trapped on a nanoViper Trap column, 2 cm x 100 μm C18 5 μm 100 Å (Thermo, 164564) then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: Mechanisms underpinning the permanent muscle damage induced by snake venom metalloprotease
    Article Snippet: The digested peptides were concentrated and separated using an Ultimate 3000 HPLC series (Dionex, USA). .. Samples were then trapped on an Acclaim PepMap 100 C18 LC column, 5um, 100A 300um i.d. x 5mm (Dionex, USA), then further separated in Nano Series Standard Columns 75μm i.d. x 15 cm.

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Mass spectrometric analysis was performed by LC-MS-MS (Liquid Chromatography-tandem Mass Spectrometry) on a Linear ion trap-orbitrap hybrid mass spectrometer (Orbitrap-VelosPro, Thermo) coupled to a U3000 RSLC HPLC (Rapid Separation/High-Performance Liquid Chromatography; Thermo). .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
    Article Snippet: The tryptic peptides were subjected to mass spectrometric analysis performed by LC‐MS‐MS on a Linear ion trap‐orbitrap hybrid mass spectrometer (Orbitrap‐VelosPro, Thermo) coupled to a U3000 RSLC HPLC (Thermo). .. Peptides were trapped on a nanoViper Trap column, 2 cm × 100 μm C18 5 μm 100 Å (Thermo, 164564), then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B [Solvent A: 2% Acetonitrile, 0.1% formic acid, 3% DMSO in H2 O; Solvent B: 80% acetonitrile, 0.08% formic acid, 3% DMSO in H2 O].

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: .. Samples for HCT116 PL1 labeling were analyzed on a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A ) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Q Exactive Plus mass spectrometer (Thermo Fisher Scientific). ..

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: .. LC-MS/MS Analysis of Proteomics Samples Samples were analyzed via LC-MS/MS using a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Thermo Fisher LTQ Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). .. Samples were loaded onto the trap column at a flow rate of 5 μL/min with aqueous 0.1% trifluoracetic acid (TFA) and then transferred onto the separation column at 0.3 μL/min.

    Article Title: Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia
    Article Snippet: Mass spectrometric analysis was performed by LC-MS-MS on a linear ion trap–orbitrap hybrid mass spectrometer (Orbitrap-VelosPro; Thermo) coupled to a U3000 RSLC HPLC (Thermo). .. Peptides were trapped on a NanoViper trap column, 2 cm × 100 mm C18 5 mm 100 Å (Thermo, 164564) and then separated on a 15-cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% solvent B [solvent A: 2% acetonitrile, 0.1% formic acid and 3% DMSO in H2 O; solvent B: 80% acetonitrile, 0.08% formic acid and 3% DMSO in H2 O].

    Flow Cytometry:

    Article Title: The DUF1669 domain of FAM83 family proteins anchor Casein Kinase 1 isoforms
    Article Snippet: .. Peptides were trapped on a nanoViper Trap column, 2 cm x 100 μm C18 5 μm 100 Å (Thermo, 164564) then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B. ..

    Article Title: Heatstroke-induced hepatocyte exosomes promote liver injury by activating the NOD-like receptor signaling pathway in mice
    Article Snippet: .. The column was equilibrated with 100% A solution (0.1% aqueous solution of FA), and the sample was loaded automatically and separated with an analytical column (Thermo Fisher Scientific, Acclaim PepMap RSLC 50 µm ×15 cm, nano viper, P/N 164943) at a flow rate of 300 nL/min. ..

    Article Title: Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes
    Article Snippet: Phosphopeptides were loaded in a μ-pre-column (Acclaim™ PepMap™ 100 C18 LC; Cat 160454, Thermo Fisher Scientific, Waltham, MA, USA) and separated in an analytical column (Acclaim™ PepMap™ 100 C18 LC; Cat. 164569, Thermo Fisher Scientific, Waltham, MA, USA) using a gradient that runs from 3% to 23% B over 120 min. .. The UPLC system delivered a flow of 2 µL/min (loading) and 300 nL/min (gradient elution).

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B. ..

    Article Title: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
    Article Snippet: .. Peptides were trapped on a nanoViper Trap column, 2 cm × 100 μm C18 5 μm 100 Å (Thermo, 164564), then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B [Solvent A: 2% Acetonitrile, 0.1% formic acid, 3% DMSO in H2 O; Solvent B: 80% acetonitrile, 0.08% formic acid, 3% DMSO in H2 O]. ..

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: LC-MS/MS Analysis of Proteomics Samples Samples were analyzed via LC-MS/MS using a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Thermo Fisher LTQ Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). .. Samples were loaded onto the trap column at a flow rate of 5 μL/min with aqueous 0.1% trifluoracetic acid (TFA) and then transferred onto the separation column at 0.3 μL/min.

    Article Title: DYRK1A Overexpression Alters Cognition and Neural-Related Proteomic Pathways in the Hippocampus That Are Rescued by Green Tea Extract and/or Environmental Enrichment
    Article Snippet: Nano-LC was equipped with a reversed-phase chromatography column of 25 cm with an inner diameter of 75 μm, packed with 3 μm C18 particles (Nikkyo Technos, NTCC-360/75-3-25L), and a Nano Trap Column Acclaim PepMap100 100 μm × 2 cm C18, 5 μm, 100 A (Thermo, 164199). .. Chromatographic gradients started at 93% of buffer A and 7% of buffer B with a flow rate of 250 nl/min during 5 min and linearly changed to 65% buffer A and 35% buffer B after 240 min. After each analysis, the column was washed for 16 min with 90% buffer A and 10% buffer B (buffer A: 0.1% formic acid in water; buffer B: 0.1% formic acid in acetonitrile).

    Article Title: The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development
    Article Snippet: .. 1 to 6 μl of peptide containing sample solution were trapped and washed with 100% solvent A (98% water, 2% acetonitrile, 0.07% trifluoroacetic acid) on an Acclaim PepMap 100 pre-column (#164564, 100 μm x 2 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) with a flow rate of 25 μl/min for 6 min. An Acclaim PepMap RSLC column (#164540, 75 μm or 50 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) was used for analytical peptide separation by reverse phase chromatography. .. This was performed by typically applying a gradient from 98% solvent A (water, 0.1% formic acid) and 2% solvent B (80% acetonitrile, 20% water, 0.1% formic acid) to 42% solvent B within 95 min and to 65% solvent B within the next 26 min at a flow rate of 300 nl/min (solvents and acids from Thermo Scientific GmbH ).

    Article Title: Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia
    Article Snippet: .. Peptides were trapped on a NanoViper trap column, 2 cm × 100 mm C18 5 mm 100 Å (Thermo, 164564) and then separated on a 15-cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% solvent B [solvent A: 2% acetonitrile, 0.1% formic acid and 3% DMSO in H2 O; solvent B: 80% acetonitrile, 0.08% formic acid and 3% DMSO in H2 O]. ..

    Article Title: C7orf59/LAMTOR4 phosphorylation and structural flexibility modulate Ragulator assembly
    Article Snippet: .. Peptides were separated running a linear gradient from 2 to 30% mobile phase B on a Acclaim™ PepMap™ 100 C18 LC Columns (Thermo Scientific), 3 µm, 150 mm length analytical column kept at 40 °C with 0.3 μL·min−1 flow over 120 min. MS data were analyzed in the Orbitrap in the range 400–2000 m/z at resolution of 120 000 (at m/z 400). ..

    Chromatography:

    Article Title: Heatstroke-induced hepatocyte exosomes promote liver injury by activating the NOD-like receptor signaling pathway in mice
    Article Snippet: Paragraph title: Easy n liquid chromatography (nLC ) chromatography ... The column was equilibrated with 100% A solution (0.1% aqueous solution of FA), and the sample was loaded automatically and separated with an analytical column (Thermo Fisher Scientific, Acclaim PepMap RSLC 50 µm ×15 cm, nano viper, P/N 164943) at a flow rate of 300 nL/min.

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Mass spectrometric analysis was performed by LC-MS-MS (Liquid Chromatography-tandem Mass Spectrometry) on a Linear ion trap-orbitrap hybrid mass spectrometer (Orbitrap-VelosPro, Thermo) coupled to a U3000 RSLC HPLC (Rapid Separation/High-Performance Liquid Chromatography; Thermo). .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: DYRK1A Overexpression Alters Cognition and Neural-Related Proteomic Pathways in the Hippocampus That Are Rescued by Green Tea Extract and/or Environmental Enrichment
    Article Snippet: Paragraph title: Liquid Chromatography-Tandem Mass Spectrometry ... Nano-LC was equipped with a reversed-phase chromatography column of 25 cm with an inner diameter of 75 μm, packed with 3 μm C18 particles (Nikkyo Technos, NTCC-360/75-3-25L), and a Nano Trap Column Acclaim PepMap100 100 μm × 2 cm C18, 5 μm, 100 A (Thermo, 164199).

    Article Title: Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44
    Article Snippet: LC–MS/MS analysis Both the PS1 and PS2 phopshoproteome and total proteome samples are analyzed on an Orbitrap Fusion Tribrid Mass spectrometer (Thermo Fisher Scientific, San Jose, U.S.A.) that was in line with the Dionex 3000 RSLC nano-liquid chromatography system, similar to previously published work [ – ]. .. The peptide samples were loaded on a nano viper trap column (C18, 5 µm, 100 Å, 100 µm × 2 cm; PN: 164562, Thermo Scientific) and resolved on a 50 cm analytical column (2 µm, 100 Å, 75 µm × 50 cm.

    SDS Page:

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Samples were filtered through a Spin-X centrifuge tube filters (Sigma, CLS8161), resolved by 4–12% gradient SDS–PAGE (Thermo Fisher Scientific, NP0323), and stained with colloidal Coomassie blue. .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
    Article Snippet: Purified proteins were resolved on 4–12% gradient gels by SDS–PAGE, and gels were subsequently stained with InstantBlue™ (Expedeon). .. Peptides were trapped on a nanoViper Trap column, 2 cm × 100 μm C18 5 μm 100 Å (Thermo, 164564), then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B [Solvent A: 2% Acetonitrile, 0.1% formic acid, 3% DMSO in H2 O; Solvent B: 80% acetonitrile, 0.08% formic acid, 3% DMSO in H2 O].

    Tandem Mass Spectroscopy:

    Article Title: Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes
    Article Snippet: Phosphopeptides were loaded in a μ-pre-column (Acclaim™ PepMap™ 100 C18 LC; Cat 160454, Thermo Fisher Scientific, Waltham, MA, USA) and separated in an analytical column (Acclaim™ PepMap™ 100 C18 LC; Cat. 164569, Thermo Fisher Scientific, Waltham, MA, USA) using a gradient that runs from 3% to 23% B over 120 min. .. The Q-Exactive Plus acquired full scan survey spectra (m/z 375–1500) with a 70,000 FWHM resolution followed by data-dependent acquisition in which the 20 most intense ions were selected for HCD (higher energy collisional dissociation) and MS/MS scanning (200–2000 m/z) with a resolution of 17,500 FWHM.

    Article Title: Mechanisms underpinning the permanent muscle damage induced by snake venom metalloprotease
    Article Snippet: Samples were then trapped on an Acclaim PepMap 100 C18 LC column, 5um, 100A 300um i.d. x 5mm (Dionex, USA), then further separated in Nano Series Standard Columns 75μm i.d. x 15 cm. .. The MS and MS/MS data were then searched against Uniprot using Sequest algorithm and the partial sequence was then compared to the other similar protein sequences available in the protein database.

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B. .. The instrument was operated with the “lock mass” option to improve the mass accuracy of precursor ions and data were acquired in the data-dependent mode, automatically switching between MS and MS-MS acquisition.

    Article Title: Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia
    Article Snippet: Peptides were trapped on a NanoViper trap column, 2 cm × 100 mm C18 5 mm 100 Å (Thermo, 164564) and then separated on a 15-cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% solvent B [solvent A: 2% acetonitrile, 0.1% formic acid and 3% DMSO in H2 O; solvent B: 80% acetonitrile, 0.08% formic acid and 3% DMSO in H2 O]. .. The instrument was operated with the “lock mass” option to improve the mass accuracy of precursor ions, and data were acquired in the data-dependent mode, automatically switching between MS and MS-MS acquisition.

    Sequencing:

    Article Title: Mechanisms underpinning the permanent muscle damage induced by snake venom metalloprotease
    Article Snippet: Samples were then trapped on an Acclaim PepMap 100 C18 LC column, 5um, 100A 300um i.d. x 5mm (Dionex, USA), then further separated in Nano Series Standard Columns 75μm i.d. x 15 cm. .. The MS and MS/MS data were then searched against Uniprot using Sequest algorithm and the partial sequence was then compared to the other similar protein sequences available in the protein database.

    Affinity Purification:

    Article Title: The DUF1669 domain of FAM83 family proteins anchor Casein Kinase 1 isoforms
    Article Snippet: Proteins were affinity purified from clarified extracts by GFP-TRAP A beads (ChromoTek) as described above. .. Peptides were trapped on a nanoViper Trap column, 2 cm x 100 μm C18 5 μm 100 Å (Thermo, 164564) then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
    Article Snippet: Mass spectrometry Proteins were affinity purified from clarified extracts by GFP TRAP beads (ChromoTek) as described above. .. Peptides were trapped on a nanoViper Trap column, 2 cm × 100 μm C18 5 μm 100 Å (Thermo, 164564), then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B [Solvent A: 2% Acetonitrile, 0.1% formic acid, 3% DMSO in H2 O; Solvent B: 80% acetonitrile, 0.08% formic acid, 3% DMSO in H2 O].

    Molecular Weight:

    Article Title: Corona Composition Can Affect the Mechanisms Cells Use to Internalize Nanoparticles
    Article Snippet: The supernatant containing the peptides was collected and dried using a speed vacuum for about 2 h. Afterward, samples were resuspended in 75 μL of 0.1% formic acid in water, and 2 μL samples were loaded into a Q Exactive Plus hybrid quadrupole–orbitrap mass spectrometer (ThermoFisher) using Acclaim PepMap 100 C18 LC columns (ThermoFisher). .. Spectral counts were normalized by the molecular weight of the identified protein and the total normalized spectral counts used to calculate the relative abundance of each protein in the corona (% of total).

    Isolation:

    Article Title: A cytoplasmic COMPASS is necessary for cell survival and triple-negative breast cancer pathogenesis by regulating metabolism
    Article Snippet: Three micrograms of each fraction was loaded onto an autosampler with a Thermo Easy nLC 1000 UPLC pump onto a vented Acclaim Pepmap 100 (75 µm × 2 cm) nanoViper trap column coupled to a 3-µm nanoViper analytical column at 100 Å, C18, 0.075 mm, and 500 mm (Thermo, 164570) with a stainless steel emitter tip assembled on the Nanospray Flex ion source with a spray voltage of 2000 V. Buffer A contained 94.785% H2 O with 5% ACN and 0.125% FA, and buffer B contained 99.875% ACN with 0.125% FA. .. Detector type was set to Orbitrap, with 60-K resolution, wide quad isolation, mass range normal, scan range 300–1500 (m/z), maximum injection time 50 msec, AGC target 200,000, 1 microscan, S-lens RF level 60, without source fragmentation, and centroid data type.

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: The isolation window was set to 1.6 m/z and the AGC target to 1.0e4 with maximum injection time of 100 ms. Ions were injected for all available parallelizable time. .. Samples for HCT116 PL1 labeling were analyzed on a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A ) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Q Exactive Plus mass spectrometer (Thermo Fisher Scientific).

    Article Title: C7orf59/LAMTOR4 phosphorylation and structural flexibility modulate Ragulator assembly
    Article Snippet: Peptides were separated running a linear gradient from 2 to 30% mobile phase B on a Acclaim™ PepMap™ 100 C18 LC Columns (Thermo Scientific), 3 µm, 150 mm length analytical column kept at 40 °C with 0.3 μL·min−1 flow over 120 min. MS data were analyzed in the Orbitrap in the range 400–2000 m/z at resolution of 120 000 (at m/z 400). .. The quadrupole was used for precursor ion isolation with window of 1.5 m/z.

    Size-exclusion Chromatography:

    Article Title: A cytoplasmic COMPASS is necessary for cell survival and triple-negative breast cancer pathogenesis by regulating metabolism
    Article Snippet: Three micrograms of each fraction was loaded onto an autosampler with a Thermo Easy nLC 1000 UPLC pump onto a vented Acclaim Pepmap 100 (75 µm × 2 cm) nanoViper trap column coupled to a 3-µm nanoViper analytical column at 100 Å, C18, 0.075 mm, and 500 mm (Thermo, 164570) with a stainless steel emitter tip assembled on the Nanospray Flex ion source with a spray voltage of 2000 V. Buffer A contained 94.785% H2 O with 5% ACN and 0.125% FA, and buffer B contained 99.875% ACN with 0.125% FA. .. The chromatographic run was for 2 h in total with the following profile: 0%–7% for 3 min, 10% for 3 min, 25% for 80 min, 33% for 20 min, 50% for 3 min, 95% for 3 min, and again 95% for 8 min. Additional MS parameters were as follows: ion transfer tube temperature 300°C, Easy-IC internal mass calibration, default charge state 2, and cycle time 3 sec.

    Labeling:

    Article Title: Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5′-Phosphate-Binding Proteome
    Article Snippet: .. Samples for HCT116 PL1 labeling were analyzed on a UltiMate 3000 nano HPLC system (Thermo Fisher Scientific) equipped with an Acclaim C18 PepMap100 75 μm ID x 2 cm trap (Thermo Fisher Scientific, Cat# ES803A ) and an Acclaim PepMap RSLC C18 separation column (75 μm ID x 50 cm, Thermo Fisher Scientific, Cat# 164535) coupled to an EASY-source equipped Q Exactive Plus mass spectrometer (Thermo Fisher Scientific). ..

    Purification:

    Article Title: A cytoplasmic COMPASS is necessary for cell survival and triple-negative breast cancer pathogenesis by regulating metabolism
    Article Snippet: For Orbitrap Fusion Tribrid MS analysis, the tryptic peptides were purified with Pierce C18 spin columns (Thermo Scientific). .. Three micrograms of each fraction was loaded onto an autosampler with a Thermo Easy nLC 1000 UPLC pump onto a vented Acclaim Pepmap 100 (75 µm × 2 cm) nanoViper trap column coupled to a 3-µm nanoViper analytical column at 100 Å, C18, 0.075 mm, and 500 mm (Thermo, 164570) with a stainless steel emitter tip assembled on the Nanospray Flex ion source with a spray voltage of 2000 V. Buffer A contained 94.785% H2 O with 5% ACN and 0.125% FA, and buffer B contained 99.875% ACN with 0.125% FA.

    Article Title: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
    Article Snippet: Purified proteins were resolved on 4–12% gradient gels by SDS–PAGE, and gels were subsequently stained with InstantBlue™ (Expedeon). .. Peptides were trapped on a nanoViper Trap column, 2 cm × 100 μm C18 5 μm 100 Å (Thermo, 164564), then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B [Solvent A: 2% Acetonitrile, 0.1% formic acid, 3% DMSO in H2 O; Solvent B: 80% acetonitrile, 0.08% formic acid, 3% DMSO in H2 O].

    Staining:

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Gels were destained in Milli-Q H2 O until background staining was minimal. .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
    Article Snippet: Purified proteins were resolved on 4–12% gradient gels by SDS–PAGE, and gels were subsequently stained with InstantBlue™ (Expedeon). .. Peptides were trapped on a nanoViper Trap column, 2 cm × 100 μm C18 5 μm 100 Å (Thermo, 164564), then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B [Solvent A: 2% Acetonitrile, 0.1% formic acid, 3% DMSO in H2 O; Solvent B: 80% acetonitrile, 0.08% formic acid, 3% DMSO in H2 O].

    Liquid Chromatography:

    Article Title: Heatstroke-induced hepatocyte exosomes promote liver injury by activating the NOD-like receptor signaling pathway in mice
    Article Snippet: Paragraph title: Easy n liquid chromatography (nLC ) chromatography ... The column was equilibrated with 100% A solution (0.1% aqueous solution of FA), and the sample was loaded automatically and separated with an analytical column (Thermo Fisher Scientific, Acclaim PepMap RSLC 50 µm ×15 cm, nano viper, P/N 164943) at a flow rate of 300 nL/min.

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Mass spectrometric analysis was performed by LC-MS-MS (Liquid Chromatography-tandem Mass Spectrometry) on a Linear ion trap-orbitrap hybrid mass spectrometer (Orbitrap-VelosPro, Thermo) coupled to a U3000 RSLC HPLC (Rapid Separation/High-Performance Liquid Chromatography; Thermo). .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development
    Article Snippet: Identification of proteins and phosphorylation sites by tandem mass spectrometry Liquid chromatography coupled either to a LCQ DecaXP mass spectrometer or an Orbitrap Velos Pro™ Hybrid Ion Trap-Orbitrap mass spectrometer (Thermo Scientific GmbH ) was employed for protein and phospho peptide identification, respectively. .. 1 to 6 μl of peptide containing sample solution were trapped and washed with 100% solvent A (98% water, 2% acetonitrile, 0.07% trifluoroacetic acid) on an Acclaim PepMap 100 pre-column (#164564, 100 μm x 2 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) with a flow rate of 25 μl/min for 6 min. An Acclaim PepMap RSLC column (#164540, 75 μm or 50 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) was used for analytical peptide separation by reverse phase chromatography.

    Software:

    Article Title: Corona Composition Can Affect the Mechanisms Cells Use to Internalize Nanoparticles
    Article Snippet: The supernatant containing the peptides was collected and dried using a speed vacuum for about 2 h. Afterward, samples were resuspended in 75 μL of 0.1% formic acid in water, and 2 μL samples were loaded into a Q Exactive Plus hybrid quadrupole–orbitrap mass spectrometer (ThermoFisher) using Acclaim PepMap 100 C18 LC columns (ThermoFisher). .. Samples were analyzed using the software PEAKS Studio 8.5 (Bioinformatics Solutions Inc.), and proteins identified using the manually reviewed UniProtKB/Swiss-Prot database.

    Reversed-phase Chromatography:

    Article Title: DYRK1A Overexpression Alters Cognition and Neural-Related Proteomic Pathways in the Hippocampus That Are Rescued by Green Tea Extract and/or Environmental Enrichment
    Article Snippet: .. Nano-LC was equipped with a reversed-phase chromatography column of 25 cm with an inner diameter of 75 μm, packed with 3 μm C18 particles (Nikkyo Technos, NTCC-360/75-3-25L), and a Nano Trap Column Acclaim PepMap100 100 μm × 2 cm C18, 5 μm, 100 A (Thermo, 164199). .. Chromatographic gradients started at 93% of buffer A and 7% of buffer B with a flow rate of 250 nl/min during 5 min and linearly changed to 65% buffer A and 35% buffer B after 240 min. After each analysis, the column was washed for 16 min with 90% buffer A and 10% buffer B (buffer A: 0.1% formic acid in water; buffer B: 0.1% formic acid in acetonitrile).

    Article Title: The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development
    Article Snippet: .. 1 to 6 μl of peptide containing sample solution were trapped and washed with 100% solvent A (98% water, 2% acetonitrile, 0.07% trifluoroacetic acid) on an Acclaim PepMap 100 pre-column (#164564, 100 μm x 2 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) with a flow rate of 25 μl/min for 6 min. An Acclaim PepMap RSLC column (#164540, 75 μm or 50 cm, C18, 3 μm, 100 Å, Thermo Scientific GmbH ) was used for analytical peptide separation by reverse phase chromatography. .. This was performed by typically applying a gradient from 98% solvent A (water, 0.1% formic acid) and 2% solvent B (80% acetonitrile, 20% water, 0.1% formic acid) to 42% solvent B within 95 min and to 65% solvent B within the next 26 min at a flow rate of 300 nl/min (solvents and acids from Thermo Scientific GmbH ).

    Concentration Assay:

    Article Title: Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia
    Article Snippet: To quench the reaction, DTT was added to a final concentration of 20 mM, and the sample was then processed on precast 4–12% gradient gel (Invitrogen). .. Peptides were trapped on a NanoViper trap column, 2 cm × 100 mm C18 5 mm 100 Å (Thermo, 164564) and then separated on a 15-cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% solvent B [solvent A: 2% acetonitrile, 0.1% formic acid and 3% DMSO in H2 O; solvent B: 80% acetonitrile, 0.08% formic acid and 3% DMSO in H2 O].

    Lysis:

    Article Title: The DUF1669 domain of FAM83 family proteins anchor Casein Kinase 1 isoforms
    Article Snippet: The expression of GFP-tagged FAM83 proteins in stable Flp-In T-Rex HEK 293 and U2OS cells was induced with 20 ng/ml doxycycline for 24 h prior to lysis. .. Peptides were trapped on a nanoViper Trap column, 2 cm x 100 μm C18 5 μm 100 Å (Thermo, 164564) then separated on a 15 cm Thermo EasySpray column (ES800) equilibrated with a flow of 300 nl/min of 3% Solvent B.

    Article Title: PathogenicFAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
    Article Snippet: Beads were washed 5 times with lysis buffer, then denatured in LDS sample buffer (Thermo Fisher Scientific, NP0007) supplemented with 2% β-mercaptoethanol. .. Peptides were trapped on a nanoViper Trap column, 2cm × 100µm C18 5µm 100Å (Thermo, 164564) then separated on a 50cm Thermo EasySpray column (ES803) equilibrated with a flow of 300 nl/min of 3% Solvent B.

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    Thermo Fisher c18 acclaim pepmap100
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    Thermo Fisher reverse phase trap column
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