acclaim pepmap100 c 18 column  (Thermo Fisher)


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    Name:
    Acclaim PepMap 100 C18 LC Columns
    Description:
    Generate high resolution analyses of tryptic natural and synthetic peptides using Thermo Scientific Acclaim PepMap 100 C18 LC Columns These columns are often used for LC MS MS peptide mapping for protein identification biomarker discovery and systems biology Due to their high loading capacity Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples Most of these C18 columns are pre assembled with Thermo Scientific nanoViper Fingertight Fittings for easy installation Classic columns are available as well
    Catalog Number:
    160321
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Chromatography Columns Resins Spin Filters
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    Structured Review

    Thermo Fisher acclaim pepmap100 c 18 column
    Generate high resolution analyses of tryptic natural and synthetic peptides using Thermo Scientific Acclaim PepMap 100 C18 LC Columns These columns are often used for LC MS MS peptide mapping for protein identification biomarker discovery and systems biology Due to their high loading capacity Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples Most of these C18 columns are pre assembled with Thermo Scientific nanoViper Fingertight Fittings for easy installation Classic columns are available as well
    https://www.bioz.com/result/acclaim pepmap100 c 18 column/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    acclaim pepmap100 c 18 column - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters
    Article Snippet: .. Eluted peptides were immediately subjected to reverse phase (RP) chromatography on a C18 analytical column (Acclaim PepMap 100 C18, 75 μm id × 25 cm, Thermo Scientific) for 150 min at a flow rate of 300 nl/min using a gradient of ACN. .. The iTRAQ-labeled peptides were analyzed by electrospray ionization (ESI) TripleTOF 5600+ System (AB Sciex).

    Article Title: Novel insights into SLC25A46-related pathologies in a genetic mouse modelBovine and murine models highlight novel roles for SLC25A46 in mitochondrial dynamics and metabolism, with implications for human and animal health
    Article Snippet: .. Ultra high pressure nanoLC 2.5 μg peptides were pre-concentrated with a flow of 3 μl/min for 10 min using a C18 trap column (Acclaim PepMap100, 100μmx2cm, Thermo Scientific) and then loaded onto a 50 cm C18 column (75 μm ID, particle size 2 μm, 100Å, Acclaim PepMap RSLC, Thermo Scientific). .. The binary pumps of the HPLC (RSLCnano, Thermo Scientific) consisted of solution A (2% v/v ACN in 0.1% v/v formic acid) and solution B (80% ACN in 0.1% formic acid).

    Purification:

    Article Title: LC-MS/MS based Serum Proteomics for Identification of Candidate Biomarkers for Hepatocellular Carcinoma
    Article Snippet: .. The purified samples were then separated using Acclaim PepMap100 C18 capillary column (75 μm id × 150 mm, 2 μm, 100Å, Dionex). .. The separation of the digests was achieved at 350 nl/min flow rate, using the following gradient: 0–10 min sustaining 5% solvent B (98% ACN with 0.1%FA), 10–65 min ramping solvent B 5–20%, 65–90 min ramping solvent B 20–30%, 90–105 min ramping solvent B 30–50%, 105–106 min ramping solvent B 50–80%, 106–110 min maintaining solvent B at 80%, 110–111 min decreasing solvent B 80–5%, and 111–120 min maintaining solvent B at 5%.

    Liquid Chromatography:

    Article Title: High-Confidence Glycosome Proteome for Procyclic Form Trypanosoma brucei by Epitope-Tag Organelle Enrichment and SILAC Proteomics
    Article Snippet: .. For experiments 1 and 2, liquid chromatography was performed on a fully automated Ultimate U3000 Nano LC System (Dionex) fitted with a 300 μm id × 5 mm Acclaim PepMap 100 C18 trap column and a 75 μm × 15 cm Acclaim PepMap C18 nanocolumn (Thermo Scientific). ..

    Mass Spectrometry:

    Article Title: Quantitative Protein Profiling of Chlamydia trachomatis Growth Forms Reveals Defense Strategies Against Tryptophan Starvation *
    Article Snippet: .. LC-MS/MS Analysis Peptides were loaded onto an Acclaim PepMap C18 precolumn (300 μm inner diameter, 5 mm long, 5 μm particle size, Dionex, Hvidovre, Denmark), and separated using an Acclaim PepMap100 C18 analytical column (75 μm inner diameter, 150 mm long, 3 μm particle size, Dionex) by a 90-min gradient controlled by a Dionex Ultimate 3000 nano-LC system connected to an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA) equipped with a nano-electrospray source (Proxeon, Odense, Denmark). .. The flow rate was 200 nL/minute; the mobile phases consisted of solvent A (2% (v/v) acetonitrile, 0.1% (v/v) formic acid) and solvent B (95% (v/v) acetonitrile, 0.1% (v/v) formic acid).

    Chromatography:

    Article Title: Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters
    Article Snippet: .. Eluted peptides were immediately subjected to reverse phase (RP) chromatography on a C18 analytical column (Acclaim PepMap 100 C18, 75 μm id × 25 cm, Thermo Scientific) for 150 min at a flow rate of 300 nl/min using a gradient of ACN. .. The iTRAQ-labeled peptides were analyzed by electrospray ionization (ESI) TripleTOF 5600+ System (AB Sciex).

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