accela 1250 hplc system  (Thermo Fisher)


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  • 95
    Name:
    Purification HPLC 1 µmol
    Description:
    Use this tool to design a custom DNA oligonucleotide with any of a variety of 3 and 5 modifications at scales ranging from 25 nmole to 10 µmole Select desired purification method normal or reversed phase chromatography HPLC or PAGE
    Catalog Number:
    10915015
    Price:
    None
    Category:
    Oligos Primers Probes Nucleotides
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    Structured Review

    Thermo Fisher accela 1250 hplc system
    Use this tool to design a custom DNA oligonucleotide with any of a variety of 3 and 5 modifications at scales ranging from 25 nmole to 10 µmole Select desired purification method normal or reversed phase chromatography HPLC or PAGE
    https://www.bioz.com/result/accela 1250 hplc system/product/Thermo Fisher
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    accela 1250 hplc system - by Bioz Stars, 2021-01
    95/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
    Article Snippet: .. DMT-off DNA is collected at 10 min. After HPLC purification, the DMT-off DNA samples are further processed by sequential dialysis in glass beakers sequentially with water, low salt (10 mM NaCl), high salt (150 mM NaCl), and water ( see Notes 11–13 ). ..

    Article Title: Cobaltacarborane-phthalocyanine conjugates: Syntheses and photophysical properties
    Article Snippet: .. HPLC purification was carried out on a Dionex system equipped with a P680 pump and a UVD340U detector with a multi-step gradient elution; A: H2 O, B: acetonitrile; 85% B (0 min)–95% B (10 min)–100% B (20 min)–100% B (37 min) 85% B (40 min). .. A semi-preparative column Luna C18 100 Å, 5 μm, 10 × 250 mm from Phenomenex, USA, was used.

    Article Title: Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectroscopy
    Article Snippet: .. After HPLC purification samples were EtOH precipitated, and resuspended in ddH2 O. DNA concentrations were determined using a NanoDrop 2000C (Thermo Scientific). .. DNA annealing reactions The biotinylated, Cy5-labeled and Cy3-labeled strands were annealed to the Tel24 abasic, 15R60/T8 hairpin abasic, or polyT abasic sequence by heating to 95°C for 4 min, followed by slow cooling to room temperature (over several hours) in the presence of a buffer (50 mM Tris–HCl, pH 8) containing either 100 mM NaCl or 100 mM KCl.

    Article Title: A Prebiotic Synthesis of Canonical Pyrimidine and Purine Ribonucleotides
    Article Snippet: .. Preparative HPLC purification was achieved with an ion exchange column (22 mm i.d., 250 mm length, 5 μm; DNAPac PA-100; Thermo Fisher Scientific) on a Waters Delta 600 module. ..

    Article Title: Immunogenicity and Efficacy of Zika Virus Envelope Domain III in DNA, Protein, and ChAdOx1 Adenoviral-Vectored Vaccines
    Article Snippet: .. The oligonucleotide consisted of 77 bases of ZIKV RNA targeted by the assay, using the GenBank accession AY632535.2 synthesized to a scale of 250 nmol through HPLC purification. ..

    Article Title: Selective AKR1C3 inhibitors do not recapitulate the anti-leukaemic activities of the pan-AKR1C inhibitor medroxyprogesterone acetate
    Article Snippet: .. HPLC purification of tetracycline breakdown product 4-MDDT 4-MDDT was purified at room temperature using a Dionex DX-600 HPLC system (Thermo Scientific, Hemel Hempstead, UK), Prevail Select C18 5 μ (250 mm × 4.6 mm i.d.) .. HPLC column.

    Article Title: Oxidation of the FAD cofactor to the 8-formyl-derivative in human electron-transferring flavoprotein
    Article Snippet: .. HPLC purification was done on a Dionex UltiMate 3000 HPLC (Thermo Fisher Scientific) equipped with an Atlantis® dC18 column (5 μm, 4.6 × 250 mm, Waters, Milford, MA) equilibrated with H2 O, 0.1% TFA, 7% acetonitrile, and a diode array detector for UV-visible monitoring (λ = 280, 370, 450, and 460 nm). ..

    Article Title: Absolute Configuration Determination of Retroflexanone Using the Advanced Mosher Method and Application of HPLC-NMR
    Article Snippet: .. HPLC Purification of Retroflexanone Semi-preparative HPLC was carried out on a Dionex P680 (solvent delivery module) equipped with a Dionex UVD340U PDA detector and a Foxy Jr. automated fraction collector. .. An isocratic HPLC method (85% CH3 CN/H2 O) was employed using a Phenomenex Luna (2) 100 Å C18 250 × 10 mm (5 µm) HPLC column.

    Purification:

    Article Title: NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
    Article Snippet: .. DMT-off DNA is collected at 10 min. After HPLC purification, the DMT-off DNA samples are further processed by sequential dialysis in glass beakers sequentially with water, low salt (10 mM NaCl), high salt (150 mM NaCl), and water ( see Notes 11–13 ). ..

    Article Title: Cobaltacarborane-phthalocyanine conjugates: Syntheses and photophysical properties
    Article Snippet: .. HPLC purification was carried out on a Dionex system equipped with a P680 pump and a UVD340U detector with a multi-step gradient elution; A: H2 O, B: acetonitrile; 85% B (0 min)–95% B (10 min)–100% B (20 min)–100% B (37 min) 85% B (40 min). .. A semi-preparative column Luna C18 100 Å, 5 μm, 10 × 250 mm from Phenomenex, USA, was used.

    Article Title: Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectroscopy
    Article Snippet: .. After HPLC purification samples were EtOH precipitated, and resuspended in ddH2 O. DNA concentrations were determined using a NanoDrop 2000C (Thermo Scientific). .. DNA annealing reactions The biotinylated, Cy5-labeled and Cy3-labeled strands were annealed to the Tel24 abasic, 15R60/T8 hairpin abasic, or polyT abasic sequence by heating to 95°C for 4 min, followed by slow cooling to room temperature (over several hours) in the presence of a buffer (50 mM Tris–HCl, pH 8) containing either 100 mM NaCl or 100 mM KCl.

    Article Title: A Prebiotic Synthesis of Canonical Pyrimidine and Purine Ribonucleotides
    Article Snippet: .. Preparative HPLC purification was achieved with an ion exchange column (22 mm i.d., 250 mm length, 5 μm; DNAPac PA-100; Thermo Fisher Scientific) on a Waters Delta 600 module. ..

    Article Title: Immunogenicity and Efficacy of Zika Virus Envelope Domain III in DNA, Protein, and ChAdOx1 Adenoviral-Vectored Vaccines
    Article Snippet: .. The oligonucleotide consisted of 77 bases of ZIKV RNA targeted by the assay, using the GenBank accession AY632535.2 synthesized to a scale of 250 nmol through HPLC purification. ..

    Article Title: Selective AKR1C3 inhibitors do not recapitulate the anti-leukaemic activities of the pan-AKR1C inhibitor medroxyprogesterone acetate
    Article Snippet: .. HPLC purification of tetracycline breakdown product 4-MDDT 4-MDDT was purified at room temperature using a Dionex DX-600 HPLC system (Thermo Scientific, Hemel Hempstead, UK), Prevail Select C18 5 μ (250 mm × 4.6 mm i.d.) .. HPLC column.

    Article Title: Oxidation of the FAD cofactor to the 8-formyl-derivative in human electron-transferring flavoprotein
    Article Snippet: .. HPLC purification was done on a Dionex UltiMate 3000 HPLC (Thermo Fisher Scientific) equipped with an Atlantis® dC18 column (5 μm, 4.6 × 250 mm, Waters, Milford, MA) equilibrated with H2 O, 0.1% TFA, 7% acetonitrile, and a diode array detector for UV-visible monitoring (λ = 280, 370, 450, and 460 nm). ..

    Article Title: Absolute Configuration Determination of Retroflexanone Using the Advanced Mosher Method and Application of HPLC-NMR
    Article Snippet: .. HPLC Purification of Retroflexanone Semi-preparative HPLC was carried out on a Dionex P680 (solvent delivery module) equipped with a Dionex UVD340U PDA detector and a Foxy Jr. automated fraction collector. .. An isocratic HPLC method (85% CH3 CN/H2 O) was employed using a Phenomenex Luna (2) 100 Å C18 250 × 10 mm (5 µm) HPLC column.

    Synthesized:

    Article Title: Immunogenicity and Efficacy of Zika Virus Envelope Domain III in DNA, Protein, and ChAdOx1 Adenoviral-Vectored Vaccines
    Article Snippet: .. The oligonucleotide consisted of 77 bases of ZIKV RNA targeted by the assay, using the GenBank accession AY632535.2 synthesized to a scale of 250 nmol through HPLC purification. ..

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